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General Information
Symbol
Dmel\MoeG0323
Species
D. melanogaster
Name
FlyBase ID
FBal0098111
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
DmoeG0323
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Insertion components
P{lacW}MoeG0323
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

R2-R5 axons do not terminate in the lamina in hemizygous third instar larvae, but instead project into the medulla. This phenotype is 100% penetrant. The morphology of the R8 growth cones is abnormal.

Homozygous R8 axon MARCM clones show severe targeting defects and have unusually large growth cones with many fine processes. Homozygous R2-R5 axon MARCM clones show appropriate targeting to the lamina region.

Imaginal discs from hemizygous MoeG0323 mutant animals are much smaller than those of wild-type animals. The number of apoptotic cells is increased in the mutant imaginal discs compared with wild-type.

Mutant animals develop more slowly than wild-type, reach pupal stages later and die as pupae.

Hemizygous males show late larval lethality. Cells in MoeG0323 imaginal discs show depletion of F-actin from the apical region (where it normally accumulates in wild-type cells) and accumulation of F-actin at ectopic sites within the cell. Cells lacking apical-basal polarity accumulate basal to the epithelial layer in MoeG0323 imaginal discs. Cells marked by Avic\GFP expression in the MoeG0323 wing disc appear to disperse from their normal position and can invade adjacent regions of the disc. The epithelial ultrastructure is disrupted in MoeG0323 wing discs; large abnormal protrusions replace the microvillar projections seen in wild-type cells.

28% of embryos from MoeEP1652/MoeG0323 mothers lack germ cells. Only 1% of embryos from MoeGT193/MoeG0323 mothers lack germ cells.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference

MoeG0323 has lethal | recessive | larval stage phenotype, suppressible by Rho172R

MoeG0323 has lethal | recessive | larval stage phenotype, suppressible by Rok1

MoeG0323 has lethal | recessive | larval stage phenotype, suppressible by Rok2

MoeG0323 has lethal | recessive | larval stage phenotype, suppressible by zip2

MoeG0323 has lethal | recessive | larval stage phenotype, suppressible by zipEbr

Enhancer of
Statement
Reference
Suppressor of
Statement
Reference

MoeG0323/Moe[+] is a suppressor of visible phenotype of dsh1

Phenotype Manifest In
Suppressed by
Statement
Reference

MoeG0323 has imaginal disc phenotype, suppressible by Rho172R

Enhancer of
Statement
Reference

MoeG0323/Moe[+] is an enhancer of medulla | third instar larval stage phenotype of wgne00637

Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Heterozygosity for MoeG0323 dramatically rescues the Syx7brd1615 terminal tracheal cell defects. The number of cysts is reduced, and terminal branching is greatly restored. The early endocytosis defects of Syx7brd1615 are not rescued by heterozygous MoeG0323.

MoeG0323/+ increases the penetrance of the axonal mistargeting defects seen in the lamina and medulla of hemizygous wgne00637 third instar larvae from 70% to 100%.

Both hemizygous MoeG0323; Rho172R/+ and homozygous MoeG0323; Rho172R/+ adult retinas have normal photoreceptors.

Halving the maternal and zygotic dose of Rho1+ (using Rho172R) strongly suppresses the lethality of MoeG0323 animals and also suppresses the MoeG0323 disc epithelium organisation and actin localisation phenotypes.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of MoeK.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4sca-109-68 rescues the R2-R5 axon targeting defects seen in MoeG0323 hemizygotes in 38% of cases.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

P{lacW} not verified as causing the lethality.

External Crossreferences and Linkouts ( 1 )
Crossreferences
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
Synonyms and Secondary IDs (5)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (15)