R2-R5 axons do not terminate in the lamina in hemizygous third instar larvae, but instead project into the medulla. This phenotype is 100% penetrant. The morphology of the R8 growth cones is abnormal.
Homozygous R8 axon MARCM clones show severe targeting defects and have unusually large growth cones with many fine processes. Homozygous R2-R5 axon MARCM clones show appropriate targeting to the lamina region.
Imaginal discs from hemizygous MoeG0323 mutant animals are much smaller than those of wild-type animals. The number of apoptotic cells is increased in the mutant imaginal discs compared with wild-type.
Mutant animals develop more slowly than wild-type, reach pupal stages later and die as pupae.
Hemizygous males show late larval lethality. Cells in MoeG0323 imaginal discs show depletion of F-actin from the apical region (where it normally accumulates in wild-type cells) and accumulation of F-actin at ectopic sites within the cell. Cells lacking apical-basal polarity accumulate basal to the epithelial layer in MoeG0323 imaginal discs. Cells marked by Avic\GFP expression in the MoeG0323 wing disc appear to disperse from their normal position and can invade adjacent regions of the disc. The epithelial ultrastructure is disrupted in MoeG0323 wing discs; large abnormal protrusions replace the microvillar projections seen in wild-type cells.