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General Information
Symbol
Dmel\pckG0012
Species
D. melanogaster
Name
FlyBase ID
FBal0098226
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
megaG0012
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Insertion of P{lacW} into the 5' untranslated region of pck, 23bp upstream of the putative translation start site, in reverse orientation.

Insertion components
P{lacW}pckG0012
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Mutant embryos have elongated trachea.

pckG0012 mutant embryos are characterised by an elongated and tortuous dorsal trunk, the main trachea, and by irregular and stretched dorsal trunk cells.

The dorsal trunk of pckG0012 homozygous embryos exhibits an elongated and convoluted lumen. The tracheal pericellular-diffusion barrier is compromised in pckG0012 mutants.

In pckG0012 mutants, the lumenal chitin in the developing dorsal trunk of the tracheal system fails to form a filamentous cable running along the tube length, as in wild-type, but instead is amorphous and fills the lumen.

Elongated and tortuous tracheal branches, most prominantly in the dorsal trunk. Pleated septate junctions are absent from tracheal cells; intercellular cleft is instead filled with unstructured electron-dense material. Germline clone analysis revealed that embryos lacking maternal pck function form a normal tracheal system and develop into fertile adults. Zygotic mutant phenotype is indistinguishable from that of embryos lacking both maternal and zygotic component pck. Transepithelial barrier function is affected - rhodamine labelled dextran diffuses across the epithelial sheet.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The tracheal length defects of kuneC309 pckG0012 sinunwu7 triple mutant embryos are no more severe than those of single mutants.

Xenogenetic Interactions
Statement
Reference

Co-expression of Zzzz\NIaScer\UAS.cHa under the control of Scer\GAL4btl.PS produces tracheal defects in flies expressing pckT:Zzzz\TEV.CS in a pckG0012 mutant background, phenocopying the pckG0012 mutant phenotype.

Complementation and Rescue Data
Rescued by
Comments

Expression of pckT:Zzzz\TEV.CS rescues the tracheal phenotypes seen in pckG0012 mutant embryos.

Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments

P{lacW} not verified as causing the lethality.

Mobilisation of the P{lacW} causes reversion of the mutant phenotype.

External Crossreferences and Linkouts ( 1 )
Crossreferences
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
Synonyms and Secondary IDs (5)
References (13)