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General Information
Symbol
Dmel\Pka-C1UAS.cKa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Kiger
FlyBase ID
FBal0098328
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-PKAc
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UASt regulatory sequences drive expression of wild type Pka-C1.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
ameliorates  obesity
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
The class IV dendritic arborization neurons in flies expressing Pka-C1Scer\UAS.cKa under the control of Scer\GAL4ppk.PG show a significant decrease in the complexity of dendritic arbors in respect to the total dendritic length. The total number of dendritic branching points is also decreased.
Flies expressing Pka-C1Scer\UAS.cKa under the control of Scer\GAL4fat have a reduced fat content compared to controls.
1.2% of egg chambers have at least one binucleate nurse cell in females expressing Pka-C1Scer\UAS.cKa under the control of Scer\GAL4nos.PG.
When Pka-C1Scer\UAS.cKa is driven by Scer\GAL4GMR.PF, the eye is discoloured and swelled up with wrinkles. The boundaries of all ommatidia and photoreceptor cells disappear.
Expression of Pka-C1Scer\UAS.cKa under the control of Scer\GAL430A results in a collapsed wing phenotype. The flies have melanotic growths in the head and show a developmental delay of about 2 days compared to controls.
When Pka-C1Scer\UAS.cKa is driven by Scer\GAL430A, wings are variably unfolded or unfolded and blistered, or collapsed. All patterns elements of the wing (veins bristles and hairs) are normal. Also melanotic tumours are often seen within the head capsule. Melanotic tumours are also found in the wing or other parts of the body. Occasionally dead or moribund flies are found that have become entirely melanized after partially opening their wings. When Pka-C1Scer\UAS.cKa is driven by Scer\GAL4684 or Scer\GAL471B, severe blistering of then wing blade is seen, accompanied by enlarged veins and a disrupted venation pattern.
Expression of insertion P{UAS-Pka-C1.K}15.3 in backgrounds Scer\GAL4RK5 and Scer\GAL4KO5 allows partial survival; 35 with a sibling class of 47, and 24 with a sibling class of 129 respectively. In the Scer\GAL4PP3 background 15.3 shows complete lethality. Expression of insertion P{UAS-Pka-C1.K}13.1 in the Scer\GAL4RK5 background allows partial survival; 3 with a sibling class of 46. In the Scer\GAL4PP3 and Scer\GAL4KO5 backgrounds 13.1 shows complete lethality. Animals weakly expressing Pka-C1Scer\UAS.cKa under the control of Scer\GAL4RK5 sometimes die as blackened pharate adults, but adults that eclose and have a variety of overlapping phenotypes are also produced. These phenotypes include warped or collapsed wings, flecks on the surface of the eye and bristles glued to the thorax. Flies that are as small as half the normal size are also seen. Some show a combination of these defects, others were normal. In the Scer\GAL4PP3 and Scer\GAL4KO5 backgrounds P{UAS-Pka-C1.K}16.5 show complete lethality. Animals weakly expressing Pka-C1Scer\UAS.cKa under the control of Scer\GAL4KO5 sometimes give rise to adults that eclose but have collapsed wings. Expression of insertions of P{UAS-Pka-C1.K} show complete lethality in the Scer\GAL4KO5, Scer\GAL4PP3, and Scer\GAL4RK5 backgrounds. Expression of insertions of P{UAS-Pka-C1.K} in a Scer\GAL4e22c background that show the strongest phenotype usually fail to hatch, and the majority develop normal larval denticle belts.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
NOT Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Additional Comments
Genetic Interactions
Statement
Reference
Expression of Pka-C1Scer\UAS.cKa under the control of Scer\GAL4fat suppresses the increased fat content seen in Lsd-11 flies.
The collapsed wing phenotype caused by expression of Pka-C1Scer\UAS.cKa under the control of Scer\GAL430A is suppressed by the presence of the transcriptionally active P-element in the P{}cactBQ insertion. The collapsed wing phenotype caused by expression of Pka-C1Scer\UAS.cKa under the control of Scer\GAL430A is suppressed by the presence of the P-Repressor encoded by P{P-Sal}89D. Mosaic analysis using the P-Repressor encoded by P{P-Sal}89D to repress expression of Pka-C1Scer\UAS.cKa under the control of Scer\GAL430A indicates that Pka-C1Scer\UAS.cKa must be expressed for the lethal interaction between bae1 and Pka-C1Scer\UAS.cKa expressed under the control of Scer\GAL430A to take place.
The addition of CrebB-17ACbz.Scer\UAS or CrebB-17AS162 has little effect on the Pka-C1Scer\UAS.cKa, Scer\GAL430A phenotypes. The addition of Stat92E06346 or Stat92Ej6C8 has little effect on the Pka-C1Scer\UAS.cKa, Scer\GAL430A phenotypes. The addition of Scer\GAL430A, strongly suppresses the wing phenotype caused by Pka-C1Scer\UAS.cKa, Scer\GAL430A alone. Fewer survivors are seen than expected, though - and those flies are weak and are trapped easily in medium. The addition of bae1 to Pka-C1Scer\UAS.cKa, Scer\GAL430A flies is lethal.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Expression of Pka-C1Scer\UAS.cKa under the control of either Scer\GAL4Mef2.247 or Scer\GAL4c309 almost completely rescues the temperature preference behavior defects seen in Pka-C1MB581/Pka-C1X4 mutant.
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Pka-C1Scer\UAS.cKa
Pka-C1UAS.cKa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Kiger
Secondary FlyBase IDs
    References (10)