FlyBase Allele Report: Dmel\Pka-C1[UAS.Tag:FLAG]

General Information

Symbol

Dmel\Pka-C1^UAS.Tag:FLAG

Species

D. melanogaster

Name

FlyBase ID

FBal0098329

Feature type

allele

Associated gene

Dmel\Pka-C1

Associated Insertion(s)

Carried in Construct

P{UAS-Pka-C1.FLAG}

Also Known As

UAS-PKA-C1, UAS-PKAcF, UAS-DC0, UAS-DC0⁺, UAS-PKA, UAS-PKA-C1-Flag, UAS-PKA-C

Key Links

Transgenic product class

genomic_DNA

(Kiger et al., 1999)

wild_type

(Kiger et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

genomic_DNA

(Kiger et al., 1999)

wild_type

(Kiger et al., 1999)

Mutagen

in vitro construct

(Kiger et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-Pka-C1.FLAG}

Cytology

Description

UASt regulatory sequences drive expression of wild type genomic Pka-C1. Also tagged with Tag:FLAG at C terminus.

(Kiger et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Pka-C1

Tagged with

Tag:FLAG

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Alzheimer's disease

modeled by Hsap\APP^{695.UAS.Tag:MYC}

(Shaw et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal memory, with Scer\GAL4^c309

(Horiuchi et al., 2008)

abnormal memory | adult stage, with Scer\GAL4^c747

(Yamazaki et al., 2014)

lethal, with Scer\GAL4^71B

(Kiger and Ho, 2001)

lethal, with Scer\GAL4^JW1

(Kiger et al., 1999)

lethal, with Scer\GAL4^KO5

(Kiger et al., 1999)

lethal, with Scer\GAL4^PP3

(Kiger et al., 1999)

partially lethal, with Scer\GAL4^RK5

(Kiger et al., 1999)

partially lethal, with Scer\GAL4^RZ4

(Kiger et al., 1999)

visible, with Scer\GAL4^RK5

(Kiger et al., 1999)

visible, with Scer\GAL4^RZ4

(Kiger et al., 1999)

Phenotype Manifest In

adult, with Scer\GAL4^RK5

(Kiger et al., 1999)

eye, with Scer\GAL4^RK5

(Kiger et al., 1999)

macrochaeta, with Scer\GAL4^RK5

(Kiger et al., 1999)

wing, with Scer\GAL4^RK5

(Kiger et al., 1999)

wing, with Scer\GAL4^RZ4

(Kiger et al., 1999)

wing margin bristle, with Scer\GAL4^RZ4

(Kiger et al., 1999)

Detailed Description

Statement

Reference

Larval fat body clones expressing Pka-C1^UAS.Tag:FLAG under the control of Scer\GAL4^Act.PU do not show significant changes in autophagosome induction in response to starvation.

(Tang et al., 2018)

Overexpressing Pka-C1^{Scer\UAS.T:Zzzz\FLAG} using Scer\GAL4^c747 disrupts 1hr memory in young flies.

(Yamazaki et al., 2014)

Expression of Pka-C1^{Scer\UAS.T:Zzzz\FLAG} using a mushroom body driver (Scer\GAL4^c309) abolishes 24-h memory after massed training in both wild-type and a Pka-C1^B3/+ heterozygous background.

Expression of Pka-C1^{Scer\UAS.T:Zzzz\FLAG} driven by Scer\GAL4^Tab2-201Y has does not effect anesthesia-resistant memory nor long-term memory in a wild-type background.

(Horiuchi et al., 2008)

Overexpression of Scer\GAL4^c819 driven by Scer\GAL4^c819 does not boost memory.

(Wang et al., 2008)

Expression of insertion P{UAS-Pka-C1.FLAG}5.5 in Scer\GAL4^RK5 background allows partial survival; 10 with a sibling class of 81. Expression of insertion P{UAS-Pka-C1.FLAG}5.9 in Scer\GAL4^RK5 background allows complete survival; 52 with a sibling class of 48. Those that survive to adulthood show a range of phenotypes, including small size, warped or collapsed wings, flecks on the surface of the eye, bristles glued to the thorax. Some show a combination of these defects, others were normal. Both P{UAS-Pka-C1.FLAG}5.5 and 5.9 show complete lethality in backgrounds Scer\GAL4^JW1, Scer\GAL4^PP3, Scer\GAL4^KO5. Insertions P{UAS-Pka-C1.FLAG}1.1, 1.3, 5.2 show complete lethality in all of the backgrounds Scer\GAL4^JW1, Scer\GAL4^PP3, Scer\GAL4^KO5, and Scer\GAL4^RK5. Expression of insertion P{UAS-Pka-C1.FLAG}5.9 in Scer\GAL4^RZ4 background produces adults 51% of which have notched wing margins.

(Kiger et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Pka-C1^UAS.Tag:FLAG, Scer\GAL4^c747 has abnormal memory | adult stage phenotype, suppressible by Pcb^EP2547/PCB[+]

(Yamazaki et al., 2014)

Pka-C1^UAS.Tag:FLAG, Scer\GAL4^RK5 has lethal phenotype, suppressible by Ocun\PKI^UAS.Tag:FLAG, Scer\GAL4^RK5

(Kiger et al., 1999)

Enhancer of

Statement

Reference

Pka-C1^UAS.Tag:FLAG, Scer\GAL4^GMR.PF is an enhancer of visible phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

Suppressor of

Statement

Reference

Pka-C1^UAS.Tag:FLAG, Scer\GAL4^c739 is a suppressor | partially of abnormal memory | adult stage phenotype of Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^c739

(Shaw et al., 2015)

Pka-C1^UAS.Tag:FLAG, Scer\GAL4^Tab2-201Y is a suppressor of chemical resistant phenotype of Pka-R1^{BDK.UAS.Tag:HA}, Scer\GAL4^Tab2-201Y

(Rodan et al., 2002)

Pka-C1^UAS.Tag:FLAG, Scer\GAL4^RK5 is a suppressor of lethal phenotype of Ocun\PKI^UAS.Tag:FLAG, Scer\GAL4^RK5

(Kiger et al., 1999)

NOT Suppressor of

Statement

Reference

Pka-C1^UAS.Tag:FLAG/Scer\GAL4^elav.PU is a non-suppressor of abnormal copulation | recessive | male phenotype of Pka-C3^d

(Cassar et al., 2018)

Pka-C1^UAS.Tag:FLAG/Scer\GAL4^{VP16.natalisin} is a non-suppressor of abnormal copulation | recessive | male phenotype of Pka-C3^d

(Cassar et al., 2018)

Phenotype Manifest In

Suppressed by

Statement

Reference

Pka-C1^UAS.Tag:FLAG, Scer\GAL4^RZ4 has wing phenotype, suppressible by Ocun\PKI^UAS.Tag:FLAG, Scer\GAL4^RZ4

(Kiger et al., 1999)

Pka-C1^UAS.Tag:FLAG, Scer\GAL4^RZ4 has wing phenotype, suppressible by Pka-R1^BDK.UAS, Scer\GAL4^RZ4

(Kiger et al., 1999)

Enhancer of

Statement

Reference

Pka-C1^UAS.Tag:FLAG, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

Pka-C1^UAS.Tag:FLAG, Scer\GAL4^GMR.PF is an enhancer of ommatidium phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

Suppressor of

Statement

Reference

Pka-C1^UAS.Tag:FLAG, Scer\GAL4^RZ4 is a suppressor of wing phenotype of Pka-R1^BDK.UAS, Scer\GAL4^RZ4

(Kiger et al., 1999)

Pka-C1^UAS.Tag:FLAG, Scer\GAL4^e22c is a suppressor | partially of denticle belt & thoracic segment phenotype of Pka-R1^BDK.UAS, Scer\GAL4^e22c

(Kiger et al., 1999)

Pka-C1^UAS.Tag:FLAG, Scer\GAL4^e22c is a suppressor of denticle belt & abdominal segment phenotype of Pka-R1^BDK.UAS, Scer\GAL4^e22c

(Kiger et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

PCB^EP2547/+ suppresses 1hr memory impairment caused by overexpressing Pka-C1^{Scer\UAS.T:Zzzz\FLAG} using Scer\GAL4^c747.

(Yamazaki et al., 2014)

Coexpression of Pka-R1^{BDK.Scer\UAS.T:Ivir\HA1} and Pka-C1^{Scer\UAS.T:Zzzz\FLAG}, both under the control of Scer\GAL4^Tab2-201Y, suppresses the reduced ethanol sensitivity phenotype of Pka-R1^{BDK.Scer\UAS.T:Ivir\HA1}.

(Rodan et al., 2002)

The P-Repressor encoded by P{P-Sal}89D has been used to suppress the lethality caused by expression of Pka-C1^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^71B. This allows mosaic analysis of the phenotype caused by expression of Pka-C1^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^71B; almost half the mosaic flies have ectopic wing veins located between veins L3 and L4 in otherwise normal wings.

(Kiger and Ho, 2001)

Coexpression of P{UAS-Pka-R1.BDK} and P{UAS-Pka-C1.FLAG} in a Scer\GAL4^e22c background rescues the abdominal denticle belt phenotype of P{UAS-Pka-R1.BDK}, and partially restores thoracic denticle belts.

(Kiger et al., 1999)

Xenogenetic Interactions

Statement

Reference

Co-expression of Pka-C1^{Scer\UAS.T:Zzzz\FLAG} significantly rescues the short term memory defects of flies expressing Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^c739.

(Shaw et al., 2015)

Co-expression of Pka-C1^{Scer\UAS.T:Zzzz\FLAG} strongly enhances the rough eye phenotype caused by expression of Hsap\MAPT^{V337M.Scer\UAS} under the control of Scer\GAL4^elav.PLu.

(Shulman and Feany, 2003)

The lethality caused by Ocun\PKI^{Scer\UAS.T:Zzzz\FLAG} or Pka-C1^{Scer\UAS.T:Zzzz\FLAG} expressed under the control of Scer\GAL4^RK5 is suppressed if they are coexpressed under the control of Scer\GAL4^RK5.

Expression of Ocun\PKI^{Scer\UAS.T:Zzzz\FLAG} suppresses the wing phenotype caused by expression of Pka-C1^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^RZ4.

(Kiger et al., 1999)

Complementation and Rescue Data