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General Information
Symbol
Dmel\Pak6
Species
D. melanogaster
Name
FlyBase ID
FBal0098342
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
dpak6
Mutagen
    Nature of the Allele
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    point mutation
    Nucleotide change:

    C6356454T

    Amino acid change:

    R113term | Pak-PA; R113term | Pak-PB; R113term | Pak-PC; R113term | Pak-PE; R113term | Pak-PF; R113term | Pak-PG; R113term | Pak-PH; R113term | Pak-PI; R113term | Pak-PJ

    Reported amino acid change:

    R113term

    Comment:

    Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference

    Mutation is in the CRIB domain.

    Amino acid replacement: R113term.

    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    Pak6/+ flies do not exhibit a significantly increased pacing-induced heart failure rate or arrhythmia, do not have significantly different diastolic or systolic intervals, nor any significant disruption in cellular architecture of cardiomyocytes as compared to controls.

    In Pak4/Pak6 mutants, the antennal lobes are smaller than those of wild type and the boundaries surrounding individual glomeruli are frequently missing.

    Pak6/Df(3R)WIN11 mutants exhibit a decrease in synaptic quantal size compared to Df(3R)WIN11/+. There is no difference in quantal content between Pak6/Df(3R)WIN11 and Df(3R)WIN11/+ mutants.

    Pak6/Pak10 mutant animals have abnormally thick axon bundles in the medulla neuropil in the developing eye and axons terminate in poorly differentiated growth cones.

    In Pak11/Pak6 adults the antennal lobes are small and mis-shapen compared to wild-type, and have amorphous neuropil. Antennal glomeruli DM2 and DM3 are severely mis-shapen or split into smaller structures that are scattered randomly around the antennal lobe. The integrity and position of VA11m is unaffected, but it is enlarged and extends into the domains of surrounding glomeruli, in most cases completely engulfing the adjacent VA1d. Projection neurons and glial cells differentiate normally in antennal lobes of the mutants, but dendritic arborization of the projection neurons is more diffuse than in wild-type, and the number of glial processes is somewhat reduced. The development of neurons within the antennae appears normal. In Pak6/Pak4 homozygous pupae 30 hours after puparium formation (hAPF) the pattern of olfactory neurons projections from the antennae is normal. However, once in the antennal lobe, the axonal trajectories are clearly abnormal: instead of forming characteristic tracks, the fibers interweave to form a dense mat.

    Motor axons in a Pak6/Pak11 mutant background exhibit guidance defects: segmental nerve (SN)b/d and SNa motor axons often (21 and 22%, respectively) overextend and bypass their target muscles.

    The subsynaptic reticulum at the NMJ between muscles 6 and 7 is reduced in size in Pak11/Pak6 third instar larvae compared to wild type.

    In Pak6/Pak11 embryos, either the entire Bolwig's Nerve, or a subset of its axons project to ectopic positions. These defects have over a 90% penetrance. About 5% of heterozygotes exhibit some Bolwig's nerve targeting defects.

    Adult escapers are uncoordinated and have crumpled wings but are otherwise wild type in appearance. R cell axons extend into the brain normally in Pak6/Pak11 flies. However these fibres do not spread evenly within the lamina and medulla. As a result some regions are hyperinnervated while others lack innervation. In the medulla neuropil, R cell axons fail to find their proper targets but instead terminate as abnormally thick, blunt ended fascicles. A small fraction of the R2-R5 neurons project through the lamina and into the medulla, indicating a modest disruption in ganglion target specificity. Some R1 to R6 axons fail to stop in the lamina, resulting in gaps in the lamina plexus. In addition, R cell axons form abnormally thick bundles between the lamina and medulla. Pak6/Pak11 mutant ommatidia are largely indistinguishable from wild-type, of 898 ommatidia counted in four Pak mutant eyes, only nine ommatidia lacked a single R cell. R cell morphology is normal, as is lamina neuron and glial cell differentiation.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    NOT Enhancer of
    Statement
    Reference

    Pak[+]/Pak6 is a non-enhancer of neuroanatomy defective phenotype of PsGEFΔ21

    Suppressor of
    Statement
    Reference

    Pak[+]/Pak6 is a suppressor | partially of lethal | larval stage phenotype of Df(3L)1226/kst2

    Pak[+]/Pak6 is a suppressor | partially of lethal | larval stage phenotype of kstB1-14.1/kst2

    NOT Suppressor of
    Statement
    Reference
    Other
    Phenotype Manifest In
    Enhanced by
    Statement
    Reference

    Pak6 has Bolwig nerve phenotype, enhanceable by Dscam105518

    Enhancer of
    Statement
    Reference

    Pak6 is an enhancer of Bolwig nerve phenotype of Dscam105518

    NOT Enhancer of
    Statement
    Reference

    Pak[+]/Pak6 is a non-enhancer of mushroom body alpha-lobe phenotype of PsGEFΔ21

    Pak6 is a non-enhancer of photoreceptor cell | precursor & nucleus phenotype of Scer\GAL4unspecified, msnDN.UAS

    Pak6 is a non-enhancer of amnioserosa phenotype of Rac1V12.UAS, Scer\GAL4hs.PB

    Suppressor of
    NOT Suppressor of
    Statement
    Reference
    Other
    Additional Comments
    Genetic Interactions
    Statement
    Reference

    Pak6/+ fully suppresses the increased satellite bouton phenotype seen in dbohenji-1/dbohenji-8 mutant larval neuromuscular junctions.

    More kst2/Df(3L)1226 mutant larvae that are also heterozygous for Pak6 survive into pupal stages and beyond than kst2/Df(3L)1226 controls.

    More kst2/kstB1-14.1 mutant larvae that are also heterozygous for Pak6 survive into pupal stages and beyond than kst2/Df(3L)1226 controls.

    Cdc423/+; Pak6/+ double heterozygotes display severe cardiomyocyte organization abnormalities and contractile abnormalities as compared with either single heterozygote, but no differences in heart beat defects and overall rhythmicity.

    Heterozygosity for Pak6 has no effect on the phenotype in the alpha lobes of the mushroom bodies that is seen in PsGEFΔ21 animals.

    0% of animals expressing msnDN.Scer\UAS in the developing eye, combined with Pak6/+ exhibit a R-cell nuclear migration phenotype.

    Pak6/+ ; GluRIIASP16/+ mutants do not exhibit a statistically significant decrease in synaptic quantal size compared to wild-type. GluRIIASP16; Pak6/+ mutants exhibit a statistically significant decrease in synaptic quantal size compared to wild-type. GluRIIASP16; Pak6/+ mutants exhibit a significant increase in quantal content compared to controls, indicating homeostatic compensation. GluRIIASP16; Pak6/Pak3 mutants exhibit a statistically significant decrease in synaptic quantal size compared to wild-type. This is a statistically significant decrease compared to GluRIIASP16. GluRIIASP16; Pak6/Pak3 mutants exhibit a significant increase in quantal content compared to controls, indicating homeostatic compensation.

    Pak6; bnl00857 double heterozygotes have a range of tracheal phenotypes not seen in single heterozygotes for either of these alleles: The mildest phenotype is a misrouting of the dorsal branches toward the anteroposterior direction; more severely effected embryos also exhibit truncations of the dorsal trunk.

    The axon guidance defects seen in Cdc42V12.Scer\UAS.T:Hsap\MYC; Scer\GAL4elav.PLu embryos are unaffected by the background Pak6/Pak11.

    About 38% of Dscam05518/Pak6 embryos exhibit some Bolwig's nerve targeting defects.

    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Rescued by
    Partially rescued by
    Not rescued by
    Comments

    Scer\GAL4SG18.1; PakScer\UAS.T:Hsap\MYC (gives expression in olfactory neurons, but not in the brain) rescues the development of most glomeruli in Pak11/Pak6 flies.

    Images (0)
    Mutant
    Wild-type
    Stocks (1)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (6)
    References (26)