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General Information
Symbol
Avic\GFPUAS.S65T.I167T.Tag:MT(btau)
Species
A. victoria
Name
FlyBase ID
FBal0098532
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Tau-GFP, tau-GFP
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of the GFP(S65T.I167T) fluorescent protein tagged with Tag:MT(btau).

Allele components
Product class / Tool use(s)
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Avic\GFPScer\UAS.S65T.I167T.T:Btau\MAPT under the control of Scer\GAL4109(2)80 in dendritic arborization (DA) neurons causes severe dendrite degeneration.

Expression of Scer\GAL4ppk.PG>Avic\GFPScer\UAS.S65T.I167T.T:Btau\MAPT engenders microtubule structure defects in dendrites.

Expression of Avic\GFPScer\UAS.S65T.I167T.T:Btau\MAPT in class IV dendritic arborizing (DA) neurons under the control of Scer\GAL4ppk.PG induces spheroid inclusion-like structures. These inclusions form preferentially at distal dendrites, although some are also present at proximal segments, causing local swelling.

Expression of Avic\GFPScer\UAS.S65T.I167T.T:Btau\MAPT under the control of Scer\GAL4smid-C161 results in defects in the central axon projections in the prothoracic neuromere in adults. Asymmetry in the projections is seen. There are four broad categories of defects in the prothoracic neuromere; reduction or loss of whole axon projections, coalescence of axons to form abnormal bundles, under-elaboration of terminal arborisations and the formation of abnormal swellings and varicosities. The medial projection of the femoral chordotonal organ (FCO) is defective in most cases, either being completely absent or massively reduced. The cell bodies of the FCO neurons are intact and appear indistinguishable from those in controls. Abnormal bundling of the sensory axons is sometimes seen in the anterior projection in the prothoracic neuromere and in the posterior projection in the metathoracic neuromere. The medial projection of the FCO is completely normal in pupae 72 hours after puparium formation in animals expressing Avic\GFPScer\UAS.S65T.I167T.T:Btau\MAPT under the control of Scer\GAL4smid-C161. Expression of Avic\GFPScer\UAS.S65T.I167T.T:Btau\MAPT under the control of Scer\GAL4en-e16E results in defects in the projections in the prothoracic neuromere in adults. Defects include loss of axons, abnormal axon bundling and beading. Expression of Avic\GFPScer\UAS.S65T.I167T.T:Btau\MAPT under the control of Scer\GAL4C42 results in axon defects identical to those caused by expression of Avic\GFPScer\UAS.S65T.I167T.T:Btau\MAPT under the control of Scer\GAL4smid-C161.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Suppressed by
Enhancer of
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

Co-expression of Hsap\LRRK2G2019S.Scer\UAS.cLa.T:Zzzz\FLAG strongly enhances the dendrite defects caused by Scer\GAL4109(2)80>Avic\GFPScer\UAS.S65T.I167T.T:Btau\MAPT. In severe cases, only six of the eight dendritic arborization (DA) neuron somas are present in the dorsal field, an indication of neuronal loss that is not detected when Hsap\LRRK2G2019S.Scer\UAS.cLa.T:Zzzz\FLAG or Avic\GFPScer\UAS.S65T.I167T.T:Btau\MAPT is expressed alone.

Co-expression of Avic\GFPScer\UAS.S65T.I167T.T:Btau\MAPT and Hsap\LRRK2G2019S.Scer\UAS.cLa.T:Zzzz\FLAG under the control of Scer\GAL4ppk.PG causes severe microtubule destruction in dendrites.

Co-expression of Hsap\LRRK2G2019S.Scer\UAS.cLa.T:Zzzz\FLAG enhances the formation of spheroid inclusions caused by Scer\GAL4ppk.PG>Avic\GFPScer\UAS.S65T.I167T.T:Btau\MAPT-expression. The spheroid inclusions appear much more frequent in both distal and proximal dendrites when Hsap\LRRK2G2019S.Scer\UAS.cLa.T:Zzzz\FLAG is also expressed.

Lrrke03680/+ partially suppresses the dendrite degeneration phenotype resulting from the expression of Scer\GAL4109(2)80>Avic\GFPScer\UAS.S65T.I167T.T:Btau\MAPT. Dendrites of the Avic\GFPScer\UAS.S65T.I167T.T:Btau\MAPT-expressing neurons are longer and the number of dendritic ends is increased. The suppression by Lrrke03680/+ is also observed in inclusion formation and microtubule fragmentation.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Avic\GFPScer\UAS.S65T.I167T.T:Btau\MAPT
Avic\GFPUAS.S65T.I167T.Tag:MT(btau)
Btau\MAPTScer\UAS.T:Avic\GFP-S65T.I167T
Name Synonyms
Secondary FlyBase IDs
    References (9)