Allele Dmel\spi^hs.PG

General Information
Symbol	Dmel\spi^hs.PG	Species	D. melanogaster
Name	heat shock construct of Guichard	FlyBase ID	FBal0098650
Feature type	allele	Associated gene	Dmel\spi

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Guichard et al., 1999)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Full length spi cDNA under the control of a heat shock promoter. (Guichard et al., 1999)
Carried in construct	P{hs-spi.m} (Guichard et al., 1999)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference Heat shock induction of spi^hs.PG throughout larval life and pupariation has no detectable phenotype in the wing. (Guichard et al., 1999)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference spi^hs.PG is an enhancer of visible phenotype of S^hs.PK, rho^hs.PSt (Guichard et al., 1999)
NOT Enhancer of
Statement Reference spi^hs.PG is a non-enhancer of visible phenotype of S^Scer\UAS.cGb, Scer\GAL4^Bx-MS1096 (Guichard et al., 1999)
NOT Suppressor of
Statement Reference spi^hs.PG is a non-suppressor of visible phenotype of S^Scer\UAS.cGb, Scer\GAL4^Bx-MS1096 (Guichard et al., 1999)
Phenotype Manifest In
NOT Enhanced by
Statement Reference spi^hs.PG has phenotype, non-enhanceable by S^hs.PK (Guichard et al., 1999)
Enhancer of
Statement Reference spi^hs.PG is an enhancer of wing phenotype of S^hs.PK, rho^hs.PSt (Guichard et al., 1999)
NOT Enhancer of
Statement Reference spi^hs.PG is a non-enhancer of phenotype of rho^hs.PSt (Guichard et al., 1999) spi^hs.PG is a non-enhancer of phenotype of S^hs.PK (Guichard et al., 1999)
NOT Suppressor of
Statement Reference spi^hs.PG is a non-suppressor of phenotype of rho^hs.PSt (Guichard et al., 1999)
Other
Statement Reference S^hs.PK, rho^hs.PSt, spi^hs.PG has eye \| anterior phenotype (Guichard et al., 1999, Guichard et al., 1999, Guichard et al., 1999) S^hs.PK, rho^hs.PSt, spi^hs.PG has wing vein phenotype (Guichard et al., 1999, Guichard et al., 1999, Guichard et al., 1999)
Additional Comments
Genetic Interactions
Statement Reference When rho^hs.PSt; spi^hs.PG flies are exposed to heat shock, no effect of elevated spi^hs.PG expression is seen beyond that attributable to rho^hs.PSt. When spi^hs.PG; S^hs.PK flies are exposed to heatshock during larval or early pupal stages no phenotype is seen. Expression of S^Scer\UAS.cGb driven by Scer\GAL4^Bx-MS1096, in a spi^hs.PG background exposed to heatshock, has no observable effect. When S^hs.PK; rho^hs.PSt; spi^hs.PG are exposed to heatshock during early pupariation (mostly between 6 and 12 hours APF) a phenotype is seen that is stronger than S^hs.PK; rho^hs.PSt alone. Also, in contrast to the diffuse ectopic veins induced by S^hs.PK; rho^hs.PSt, a sharp pattern of well defined forked longitudinal vein is observed. A phenotype can also be seen in the eye, in which cell death is localised at the anterior part of the eye, when heat shock is provided between 0 and 6 hours APF. (Guichard et al., 1999)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescues	spi^hs.PG partially rescues spi¹/spi³ (Guichard et al., 1999)
Comments	Heat shock expression of spi^hs.PG in spi¹/spi³ mutants can partially rescue lethality and reduces the severity of the spi¹/spi³ mutant phenotype in remaining dead embryos. (Guichard et al., 1999)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	spi^hs.PG
Name Synonym	heat shock construct of Guichard (Guichard et al., 1999)
Secondary FlyBase IDs

References ( 1 )
Research paper	Guichard et al., 1999, Development 126(12): 2663--2676 rhomboid and Star interact synergistically to promote EGFR/MAPK signaling during Drosophila wing vein development. [FBrf0108778]

Allele Dmel\spihs.PG

Allele Dmel\spi^hs.PG