FlyBase Allele Report: Dmel\ovo[UAS.cPa]

General Information

Symbol

Dmel\ovo^UAS.cPa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Payre

FlyBase ID

FBal0098674

Feature type

allele

Associated gene

Dmel\ovo

Associated Insertion(s)

Carried in Construct

P{UAS-ovo.P}

Key Links

Transgenic product class

cDNA

(Payre et al., 1999)

wild_type

(Payre et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Payre et al., 1999)

wild_type

(Payre et al., 1999)

Mutagen

in vitro construct

(Payre et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-ovo.P}

Cytology

Description

The ovo embryonic "svb" cDNA under the control of UASt regulatory sequences.

(Payre et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

ovo

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

denticle | ectopic, with Scer\GAL4^en-e16E

(Payre et al., 1999)

denticle | ectopic, with Scer\GAL4^ptc-559.1

(Payre et al., 1999)

denticle | ectopic, with Scer\GAL4^wg.PM

(Payre et al., 1999)

embryonic epidermis, with Scer\GAL4^en-e16E

(Payre et al., 1999)

embryonic epidermis, with Scer\GAL4^ptc-559.1

(Payre et al., 1999)

embryonic epidermis, with Scer\GAL4^wg.PM

(Payre et al., 1999)

filamentous actin & embryonic/first instar larval cuticle | ectopic, with Scer\GAL4^ptc-559.1

(Walters et al., 2006)

filamentous actin & embryonic/first instar larval cuticle | ectopic | somatic clone, with Scer\GAL4^Act5C.PI

(Walters et al., 2006)

ventral denticle belt, with Scer\GAL4^en-e16E

(Payre et al., 1999)

ventral denticle belt, with Scer\GAL4^ptc-559.1

(Payre et al., 1999)

ventral denticle belt, with Scer\GAL4^wg.PM

(Payre et al., 1999)

Detailed Description

Statement

Reference

When ovo^Scer\UAS.cPa is expressed in the prospective smooth cells of embryos, by induction of clones under the control of Scer\GAL4^Act5C.PI, ectopic actin-based protrusions are formed within these cells. However, unlike in denticle cells where these protrusions normally occur, ectopic protrusions are in smooth cells are not preferentially localized to the posterior edge of cells. This phenotype is also seen when ovo^Scer\UAS.cPa is expressed under the control of Scer\GAL4^ptc-559.1.

(Walters et al., 2006)

ovo^Scer\UAS.cPa driven by Scer\GAL4^en-e16E induces the formation of an extra row of denticles in anterior en expressing cells. The morphology of denticles normally present in the posterior en expressing cells is unaffected by ovo overexpression. Expression of ovo^Scer\UAS.cPa driven by Scer\GAL4^ptc-559.1 in a ovo^svb-2 background also produces ectopic denticles. Expression of ovo^Scer\UAS.cPa by Scer\GAL4^wg.PM produces denticles in wg expressing cells.

(Payre et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

ovo^UAS.cPa, Scer\GAL4^e22c is a suppressor of embryonic epidermis phenotype of Egfr^DN.UAS, Scer\GAL4^e22c

(Payre et al., 1999)

ovo^UAS.cPa, Scer\GAL4^e22c is a suppressor of ventral denticle belt phenotype of Egfr^DN.UAS, Scer\GAL4^e22c

(Payre et al., 1999)

ovo^UAS.cPa, Scer\GAL4^69B is a suppressor of embryonic epidermis phenotype of Egfr^DN.UAS, Scer\GAL4^69B

(Payre et al., 1999)

ovo^UAS.cPa, Scer\GAL4^69B is a suppressor of ventral denticle belt phenotype of Egfr^DN.UAS, Scer\GAL4^69B

(Payre et al., 1999)

ovo^UAS.cPa/Scer\GAL4^e22c is a suppressor of embryonic epidermis phenotype of Scer\GAL4^e22c, arm^{S10.UAS.Tag:MYC}

(Payre et al., 1999)

ovo^UAS.cPa/Scer\GAL4^e22c is a suppressor of ventral denticle belt phenotype of Scer\GAL4^e22c, arm^{S10.UAS.Tag:MYC}

(Payre et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of ovo^Scer\UAS.cPa in naked cuticle driven by Scer\GAL4^ptc-559.1 produces ectopic denticles, but does not affect the normal denticle rows. Uniform expression of ovo^Scer\UAS.cPa in a arm^{S10.Scer\UAS.T:Hsap\MYC}, Scer\GAL4^e22c background restores denticle formation, suppressing the naked cuticle phenotype caused by ectopic arm^{S10.Scer\UAS.T:Hsap\MYC} expression. Co-expression of Egfr^DN.Scer\UAS and ovo^D1.Scer\UAS driven by Scer\GAL4^e22c or Scer\GAL4^69B suppresses the loss of denticle belt phenotype seen by ubiquitous expression of Egfr^DN.Scer\UAS alone.

(Payre et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

ovo^Scer\UAS.cPa

ovo^UAS.cPa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Payre

(Payre et al., 1999)

Secondary FlyBase IDs

References (2)

Report Sections

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