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General Information
Symbol
Dmel\dncUAS.cCa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Cheung
FlyBase ID
FBal0098748
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-dnc, UAS-dunce, UAS-dnc+
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a dnc cDNA.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of dncScer\UAS.cCa under the control of Scer\GAL4rk.pan results in intestinal stem cell hyperproliferation.

Flies expressing dncScer\UAS.cCa under the control of Scer\GAL4tim.PE are arrhythmic for locomotor activity under constant darkness conditions. Under 12 hour light/12 hour dark conditions the phase of the evening peak of activity is advanced and morning anticipation is reduced in these flies.

Third instar larvae expressing dncScer\UAS.cCa under the control of Scer\GAL4Tdc2.PC show a significant decrease in the number of natural synaptopods (motile filopodia-like extensions) and number of type II boutons at the type II neuromuscular junction compared to wild type.

Flies expressing dncScer\UAS.cCa under the control of two copies of Scer\GAL4P0.5.Pdf in constant darkness exhibit much longer periods than control flies (26.6hr versus 24.0 hr, respectively). Those with only one copy of Scer\GAL4P0.5.Pdf exhibit slightly shorter periods of 25.5hrs.

Expression of dncScer\UAS.cCa under the control of Scer\GAL4131a results in adults which show abnormal responses to acetone and ethanol and normal responses to ethyl acetate in an olfactory preference test. Expression of dncScer\UAS.cCa under the control of Scer\GAL4272 results in adults which show normal responses to ethyl acetate, acetone and acetic acid in an olfactory preference test. Expression of dncScer\UAS.cCa under the control of Scer\GAL4345 results in adults which show normal responses to ethyl acetate, acetone and ethanol in an olfactory preference test. Expression of dncScer\UAS.cCa under the control of Scer\GAL4148a, Scer\GAL4179a or Scer\GAL4250 results in adults which show abnormal responses to ethanol and normal responses to ethyl acetate and acetone in an olfactory preference test. Expression of dncScer\UAS.cCa under the control of Scer\GAL4555 results in adults which show abnormal responses to acetone and normal responses to ethyl acetate and ethanol in an olfactory preference test. Expression of dncScer\UAS.cCa under the control of Scer\GAL4588 results in adults which show normal responses to ethyl acetate, acetone and propionaldehyde in an olfactory preference test.

Expression of dncScer\UAS.cCa under the control of Scer\GAL4elav-C155 has no effect on synapse size (bouton number) or strength (EJC amplitude) at the larval neuromuscular junction.

Larvae expressing dncScer\UAS.cCa under the control of Scer\GAL4D42 have 40-50% fewer type I varicosities at the neuromuscular junction of muscles 6 and 7 than normal. The mean synaptic area for synapses in individual type Ib and Is varicosities is significantly increased in animals expressing dncScer\UAS.cCa under the control of Scer\GAL4D42 compared to controls. The number of dense bodies per synapse is modestly increased in type Ib but not in type Is varicosities.

Expression of dncScer\UAS.cCa under the control of Scer\GAL4D42 results in 40-50% fewer type Ib and type Is varicosities at the neuromuscular junction of muscles 6 and 7 of abdominal segment 4 in larvae. There is also a striking reduction (to approximately 40% of wild type) in the length of type II terminals and the number of varicosities at the neuromuscular junction of muscles 12 and 13. There is a reduction in excitatory junction potential (EJP) amplitude at muscles 6 and 7 compared to wild type. The frequency of miniature EJPs (mEJPs) at muscle 6 is reduced.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
Enhancer of
Suppressor of
NOT Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Enhancer of
Statement
Reference
Suppressor of
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

The reduction in the number of natural synaptopods (motile filopodia-like extensions) and number of type II boutons at the type II neuromuscular junction which is seen in third instar larvae expressing dncScer\UAS.cCa under the control of Scer\GAL4Tdc2.PC is not altered if the larvae are also homozygous for oa21.

The arrhythmicity caused by expression of G-sα60AQ215L.Scer\UAS in LN[[v]] neurons under the control of Scer\GAL4P0.5.Pdf in constant darkness is completely suppressed by co-expression of dncScer\UAS.cCa. These flies also exhibit ~2hr shorter periods than flies expressing dncScer\UAS.cCa alone.

Co-expression of dncScer\UAS.cCa with G-oα47AGTP.Scer\UAS in LN[[v]] neurons under the control of Scer\GAL4P0.5.Pdf results in low power short-period rhythms.

Homozygous Ent2P124 early third instar larvae expressing dncScer\UAS.cCa under the control of Scer\GAL4elav.Switch.PO upon induction by RU486 (mifepristone) display more severe synaptic defects than do Ent2P124 mutants without dncScer\UAS.cCa-overexpression.

Pre-synaptic expression of dncScer\UAS.cCa (under the control of Scer\GAL4elav-C155 in a sei2 background severely reduces the number of type M, but not type B, satellites. There is also a milder decrease in primary bouton and branch numbers.

Pre-synaptic expression of dncScer\UAS.cCa (under the control of Scer\GAL4elav-C155 in a slo1 background does not influence satellite bouton number.

Post-synaptic expression of dncScer\UAS.cCa (under the control of Scer\GAL4C164 in a slo1 background suppresses the number of both type M and type B satellites. However, the number of mature boutons remains higher in these mutants.

Expression of dncScer\UAS.cCa, under the control of Scer\GAL4eve.RN2O, in aCC/RP2 neurons with an Acej50 background suppresses the reduction of inward Na+ current and the reduction in excitability seen in Acej50 alone.

Expression of dncScer\UAS.cCa under the control of Scer\GAL4elav-C155 does not change the effect of co-expression of kayScer\UAS.cEa and JraScer\UAS.cEa (under the control of Scer\GAL4elav-C155) on bouton number, but significantly inhibits the effect of co-expression of kayScer\UAS.cEa and JraScer\UAS.cEa (under the control of Scer\GAL4elav-C155) on EJC amplitude (synaptic strength); the triple mutant flies have an EJC amplitude 83% that of control flies.

Xenogenetic Interactions
Statement
Reference

Flies expressing dncScer\UAS.cCa and Bper\ptxAact.Scer\UAS in LN[[v]]s under the control of Scer\GAL4P0.5.Pdf have an average period of 28.4 hours, significantly longer than either single transgene alone.

Complementation and Rescue Data
Comments

Expression of dncScer\UAS.cCa under the control of Scer\GAL4elav.PU rescues the defective short term memory performance of dnc1/dnc1 mutants, whether expressed throughout development or with expression restricted to adult stage with the use of a Gal80[ts] transgene.

Expression of dncScer\UAS.cCa under the control of both Scer\GAL4GH298 and Scer\GAL4ey-OK107, both Scer\GAL4GH298 and Scer\GAL417d, both Scer\GAL4GH298 and Scer\GAL4NP1131, both Scer\GAL4GH298 and Scer\GAL4c320, both Scer\GAL4GH298 and Scer\GAL4c305a, both Scer\GAL4GH298 and Scer\GAL4Mef2.247, or both Scer\GAL4NP1227 and Scer\GAL4Mef2.247 (but not under the control of any one of Scer\GAL4GH298, Scer\GAL4NP1227, Scer\GAL4Orco.PU, Scer\GAL4Mef2.247, or Scer\GAL4NP0225 alone) rescues the defective short term memory performance of dnc1/dnc1 mutants.

Expression of dncScer\UAS.cCa under the control of both Scer\GAL4GH298 and Scer\GAL417d, both Scer\GAL4GH298 and Scer\GAL4NP1131, both Scer\GAL4GH298 and Scer\GAL4c305a, or both Scer\GAL4GH298 and Scer\GAL4Mef2.247 (but not under the control of one of Scer\GAL4Mef2.247 or Scer\GAL4GH298 alone) rescues the defective anaesthesia-resistant memory performance of dnc1/dnc1 mutants.

Expression of dncScer\UAS.cCa exclusively in octopaminergic neurons under the control of Scer\GAL4Tdc2.PC rescues the increase in synaptopods at neuromuscular junction type II endings seen in dncM14 or dncML homozygous and dncM14/dncML heterozygous mutants.

Expression of dncScer\UAS.cCa using Scer\GAL4rafl-1 or Scer\GAL4c309 rescues the aberrant brain responses to novelty seen in dnc1 flies. Use of Scer\GAL4val-1 results in partial rescue of this phenotype.

Expression of dncScer\UAS.cCa using Scer\GAL4toi-1 does not rescue the aberrant brain responses to novelty seen in dnc1 flies.

Expression of dncScer\UAS.cCa using Scer\GAL4c309, but not Scer\GAL4val-1 or Scer\GAL4toi-1, rescues the defective optomotor behaviour of dnc1 flies.

Expression of dncScer\UAS.cCa induced using Scer\GAL4elav.Switch.PO at a late pupal stage is sufficient to rescue the aberrant brain responses to novelty seen in dnc1 flies.

Expression of dncScer\UAS.cCa under the control of Scer\GAL4elav.Switch.PO (in the presence of RU486 to activate the Scer\GAL4elav.Switch.PO driver) in dnc1 flies decreases the optomotor responsiveness to wild-type levels and increases brain responsiveness to novelty to wild-type levels.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
dncScer\UAS.cCa
dncUAS.cCa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Cheung
Secondary FlyBase IDs
    References (17)