FlyBase Allele Report: Dmel\Pde4[UAS.cCa]

General Information

Symbol

Dmel\Pde4^UAS.cCa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Cheung

FlyBase ID

FBal0098748

Feature type

allele

Associated gene

Dmel\Pde4

Associated Insertion(s)

Carried in Construct

P{UAS-Pde4.C}

Also Known As

UAS-dnc, UAS-dunce, UAS-dnc⁺

Key Links

Transgenic product class

cDNA

(Cheung et al., 1999)

wild_type

(Cheung et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Cheung et al., 1999)

wild_type

(Cheung et al., 1999)

Mutagen

in vitro construct

(Cheung et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-Pde4.C}

Cytology

Description

UASt regulatory sequences drive expression of a dnc cDNA.

(Cheung et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Pde4

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal circadian rhythm, with Scer\GAL4^P0.5.Pdf

(Dahdal et al., 2010)

abnormal locomotor rhythm, with Scer\GAL4^tim.PE

(Zhang and Emery, 2013)

abnormal neuroanatomy, with Scer\GAL4^Toll-6-D42

(Shayan and Atwood, 2000)

abnormal neuroanatomy | larval stage, with Scer\GAL4^Toll-6-D42

(Cheung et al., 1999)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^Tdc2.PC

(Koon and Budnik, 2012)

abnormal neurophysiology | larval stage, with Scer\GAL4^Toll-6-D42

(Cheung et al., 1999)

abnormal smell perception, with Scer\GAL4^131a

(Gomez-Diaz et al., 2004)

abnormal smell perception, with Scer\GAL4^148a

(Gomez-Diaz et al., 2004)

abnormal smell perception, with Scer\GAL4^179a

(Gomez-Diaz et al., 2004)

abnormal smell perception, with Scer\GAL4²⁵⁰

(Gomez-Diaz et al., 2004)

abnormal smell perception, with Scer\GAL4⁵⁵⁵

(Gomez-Diaz et al., 2004)

increased occurrence of cell division | adult stage, with Scer\GAL4^rk.pan

(Scopelliti et al., 2014)

Phenotype Manifest In

adult midgut, with Scer\GAL4^rk.pan

(Scopelliti et al., 2014)

dense body, with Scer\GAL4^Toll-6-D42

(Shayan and Atwood, 2000)

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^Tdc2.PC

(Koon and Budnik, 2012)

intestinal stem cell | adult stage, with Scer\GAL4^rk.pan

(Scopelliti et al., 2014)

neuromuscular junction, with Scer\GAL4^Toll-6-D42

(Shayan and Atwood, 2000)

neuromuscular junction & abdominal 4 ventral longitudinal muscle 3 & larva, with Scer\GAL4^Toll-6-D42

(Cheung et al., 1999)

neuromuscular junction & abdominal 4 ventral longitudinal muscle 4 & larva, with Scer\GAL4^Toll-6-D42

(Cheung et al., 1999)

neuromuscular junction & abdominal ventral longitudinal muscle 1 & larva, with Scer\GAL4^Toll-6-D42

(Cheung et al., 1999)

neuromuscular junction & abdominal ventral longitudinal muscle 2 & larva, with Scer\GAL4^Toll-6-D42

(Cheung et al., 1999)

neuromuscular junction | larval stage, with Scer\GAL4^Toll-6-D42

(Cheung et al., 1999)

synapse, with Scer\GAL4^Toll-6-D42

(Shayan and Atwood, 2000)

type II bouton | third instar larval stage, with Scer\GAL4^Tdc2.PC

(Koon and Budnik, 2012)

Detailed Description

Statement

Reference

Expression of dnc^Scer\UAS.cCa under the control of Scer\GAL4^rk.pan results in intestinal stem cell hyperproliferation.

(Scopelliti et al., 2014)

Flies expressing dnc^Scer\UAS.cCa under the control of Scer\GAL4^tim.PE are arrhythmic for locomotor activity under constant darkness conditions. Under 12 hour light/12 hour dark conditions the phase of the evening peak of activity is advanced and morning anticipation is reduced in these flies.

(Zhang and Emery, 2013)

Third instar larvae expressing dnc^Scer\UAS.cCa under the control of Scer\GAL4^Tdc2.PC show a significant decrease in the number of natural synaptopods (motile filopodia-like extensions) and number of type II boutons at the type II neuromuscular junction compared to wild type.

(Koon and Budnik, 2012)

Flies expressing dnc^Scer\UAS.cCa under the control of two copies of Scer\GAL4^P0.5.Pdf in constant darkness exhibit much longer periods than control flies (26.6hr versus 24.0 hr, respectively). Those with only one copy of Scer\GAL4^P0.5.Pdf exhibit slightly shorter periods of 25.5hrs.

(Dahdal et al., 2010)

Expression of dnc^Scer\UAS.cCa under the control of Scer\GAL4^131a results in adults which show abnormal responses to acetone and ethanol and normal responses to ethyl acetate in an olfactory preference test. Expression of dnc^Scer\UAS.cCa under the control of Scer\GAL4²⁷² results in adults which show normal responses to ethyl acetate, acetone and acetic acid in an olfactory preference test. Expression of dnc^Scer\UAS.cCa under the control of Scer\GAL4³⁴⁵ results in adults which show normal responses to ethyl acetate, acetone and ethanol in an olfactory preference test. Expression of dnc^Scer\UAS.cCa under the control of Scer\GAL4^148a, Scer\GAL4^179a or Scer\GAL4²⁵⁰ results in adults which show abnormal responses to ethanol and normal responses to ethyl acetate and acetone in an olfactory preference test. Expression of dnc^Scer\UAS.cCa under the control of Scer\GAL4⁵⁵⁵ results in adults which show abnormal responses to acetone and normal responses to ethyl acetate and ethanol in an olfactory preference test. Expression of dnc^Scer\UAS.cCa under the control of Scer\GAL4⁵⁸⁸ results in adults which show normal responses to ethyl acetate, acetone and propionaldehyde in an olfactory preference test.

(Gomez-Diaz et al., 2004)

Expression of dnc^Scer\UAS.cCa under the control of Scer\GAL4^elav-C155 has no effect on synapse size (bouton number) or strength (EJC amplitude) at the larval neuromuscular junction.

(Sanyal et al., 2002)

Larvae expressing dnc^Scer\UAS.cCa under the control of Scer\GAL4^D42 have 40-50% fewer type I varicosities at the neuromuscular junction of muscles 6 and 7 than normal. The mean synaptic area for synapses in individual type Ib and Is varicosities is significantly increased in animals expressing dnc^Scer\UAS.cCa under the control of Scer\GAL4^D42 compared to controls. The number of dense bodies per synapse is modestly increased in type Ib but not in type Is varicosities.

(Shayan and Atwood, 2000)

Expression of dnc^Scer\UAS.cCa under the control of Scer\GAL4^D42 results in 40-50% fewer type Ib and type Is varicosities at the neuromuscular junction of muscles 6 and 7 of abdominal segment 4 in larvae. There is also a striking reduction (to approximately 40% of wild type) in the length of type II terminals and the number of varicosities at the neuromuscular junction of muscles 12 and 13. There is a reduction in excitatory junction potential (EJP) amplitude at muscles 6 and 7 compared to wild type. The frequency of miniature EJPs (mEJPs) at muscle 6 is reduced.

(Cheung et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Pde4^UAS.cCa, Scer\GAL4^P0.5.Pdf has abnormal circadian rhythm phenotype, enhanceable by Bper\ptxA^act.UAS, Scer\GAL4^P0.5.Pdf

(Dahdal et al., 2010)

Suppressed by

Statement

Reference

Pde4^UAS.cCa, Scer\GAL4^P0.5.Pdf has abnormal circadian rhythm phenotype, suppressible by Pde4^UAS.cCa, Scer\GAL4^P0.5.Pdf

(Dahdal et al., 2010)

Enhancer of

Statement

Reference

Pde4^UAS.cCa, Scer\GAL4^P0.5.Pdf is an enhancer of abnormal circadian rhythm phenotype of Bper\ptxA^act.UAS, Scer\GAL4^P0.5.Pdf

(Dahdal et al., 2010)

Scer\GAL4^{elav.Switch.PO}/Pde4^UAS.cCa is an enhancer of abnormal neurophysiology | third instar larval stage | recessive phenotype of Ent2^P124

(Knight et al., 2010)

Suppressor of

Statement

Reference

Pde4^UAS.cCa, Scer\GAL4^P0.5.Pdf is a suppressor of abnormal circadian rhythm phenotype of Pde4^UAS.cCa, Scer\GAL4^P0.5.Pdf

(Dahdal et al., 2010)

Pde4^UAS.cCa, Scer\GAL4^P0.5.Pdf is a suppressor of abnormal circadian rhythm phenotype of Gαs^Q215L.UAS, Scer\GAL4^P0.5.Pdf

(Dahdal et al., 2010)

Pde4^UAS.cCa/Scer\GAL4^C164 is a suppressor of abnormal neuroanatomy phenotype of slo¹

(Lee and Wu, 2010)

Scer\GAL4^elav-C155/Pde4^UAS.cCa is a suppressor of abnormal neuroanatomy phenotype of sei²

(Lee and Wu, 2010)

Pde4^UAS.cCa/Scer\GAL4^eve.RN2O is a suppressor of abnormal neurophysiology | dominant phenotype of Ace^j50

(Baines, 2003)

Pde4^UAS.cCa, Scer\GAL4^elav-C155 is a suppressor | partially of abnormal neurophysiology phenotype of Jra^UAS.cEa, Scer\GAL4^elav-C155, kay^UAS.cEa

(Sanyal et al., 2002)

NOT Suppressor of

Statement

Reference

Scer\GAL4^elav-C155/Pde4^UAS.cCa is a non-suppressor of abnormal neuroanatomy phenotype of slo¹

(Lee and Wu, 2010)

Other

Statement

Reference

Gαo^GTP.UAS, Pde4^UAS.cCa, Scer\GAL4^P0.5.Pdf has abnormal circadian rhythm phenotype

(Dahdal et al., 2010)

Phenotype Manifest In

Enhancer of

Statement

Reference

Scer\GAL4^{elav.Switch.PO}/Pde4^UAS.cCa is an enhancer of synapse phenotype of Ent2^P124

(Knight et al., 2010)

Suppressor of

Statement

Reference

Pde4^UAS.cCa/Scer\GAL4^C164 is a suppressor of NMJ bouton | increased number phenotype of slo¹

(Lee and Wu, 2010)

Pde4^UAS.cCa/Scer\GAL4^C164 is a suppressor of neuromuscular junction phenotype of slo¹

(Lee and Wu, 2010)

Scer\GAL4^elav-C155/Pde4^UAS.cCa is a suppressor of NMJ bouton | increased number phenotype of sei²

(Lee and Wu, 2010)

Scer\GAL4^elav-C155/Pde4^UAS.cCa is a suppressor of neuromuscular junction phenotype of sei²

(Lee and Wu, 2010)

Pde4^UAS.cCa, Scer\GAL4^elav-C155 is a suppressor | partially of neuromuscular junction | larval stage phenotype of Jra^UAS.cEa, Scer\GAL4^elav-C155, kay^UAS.cEa

(Sanyal et al., 2002)

NOT Suppressor of

Statement

Reference

Scer\GAL4^elav-C155/Pde4^UAS.cCa is a non-suppressor of NMJ bouton | increased number phenotype of slo¹

(Lee and Wu, 2010)

Scer\GAL4^elav-C155/Pde4^UAS.cCa is a non-suppressor of neuromuscular junction phenotype of slo¹

(Lee and Wu, 2010)

Pde4^UAS.cCa, Scer\GAL4^elav-C155 is a non-suppressor of bouton | increased number phenotype of Jra^UAS.cEa, Scer\GAL4^elav-C155, kay^UAS.cEa

(Sanyal et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

The reduction in the number of natural synaptopods (motile filopodia-like extensions) and number of type II boutons at the type II neuromuscular junction which is seen in third instar larvae expressing dnc^Scer\UAS.cCa under the control of Scer\GAL4^Tdc2.PC is not altered if the larvae are also homozygous for oa2¹.

(Koon and Budnik, 2012)

The arrhythmicity caused by expression of G-sα60A^{Q215L.Scer\UAS} in LN[[v]] neurons under the control of Scer\GAL4^P0.5.Pdf in constant darkness is completely suppressed by co-expression of dnc^Scer\UAS.cCa. These flies also exhibit ~2hr shorter periods than flies expressing dnc^Scer\UAS.cCa alone.

Co-expression of dnc^Scer\UAS.cCa with G-oα47A^GTP.Scer\UAS in LN[[v]] neurons under the control of Scer\GAL4^P0.5.Pdf results in low power short-period rhythms.

(Dahdal et al., 2010)

Homozygous Ent2^P124 early third instar larvae expressing dnc^Scer\UAS.cCa under the control of Scer\GAL4^{elav.Switch.PO} upon induction by RU486 (mifepristone) display more severe synaptic defects than do Ent2^P124 mutants without dnc^Scer\UAS.cCa-overexpression.

(Knight et al., 2010)

Pre-synaptic expression of dnc^Scer\UAS.cCa (under the control of Scer\GAL4^elav-C155 in a sei² background severely reduces the number of type M, but not type B, satellites. There is also a milder decrease in primary bouton and branch numbers.

Pre-synaptic expression of dnc^Scer\UAS.cCa (under the control of Scer\GAL4^elav-C155 in a slo¹ background does not influence satellite bouton number.

Post-synaptic expression of dnc^Scer\UAS.cCa (under the control of Scer\GAL4^C164 in a slo¹ background suppresses the number of both type M and type B satellites. However, the number of mature boutons remains higher in these mutants.

(Lee and Wu, 2010)

Expression of dnc^Scer\UAS.cCa, under the control of Scer\GAL4^eve.RN2O, in aCC/RP2 neurons with an Ace^j50 background suppresses the reduction of inward Na⁺ current and the reduction in excitability seen in Ace^j50 alone.

(Baines, 2003)

Expression of dnc^Scer\UAS.cCa under the control of Scer\GAL4^elav-C155 does not change the effect of co-expression of kay^Scer\UAS.cEa and Jra^Scer\UAS.cEa (under the control of Scer\GAL4^elav-C155) on bouton number, but significantly inhibits the effect of co-expression of kay^Scer\UAS.cEa and Jra^Scer\UAS.cEa (under the control of Scer\GAL4^elav-C155) on EJC amplitude (synaptic strength); the triple mutant flies have an EJC amplitude 83% that of control flies.

(Sanyal et al., 2002)

Xenogenetic Interactions

Statement

Reference

Flies expressing dnc^Scer\UAS.cCa and Bper\ptxA^act.Scer\UAS in LN[[v]]s under the control of Scer\GAL4^P0.5.Pdf have an average period of 28.4 hours, significantly longer than either single transgene alone.

(Dahdal et al., 2010)

Complementation and Rescue Data

Rescues

Pde4^UAS.cCa/Scer\GAL4^elav.PU rescues Pde4^dnc-1

(Scheunemann et al., 2012)

Scer\GAL4^GH298, Pde4^UAS.cCa, Scer\GAL4^ey-OK107 rescues Pde4^dnc-1

(Scheunemann et al., 2012)

Scer\GAL4^GH298, Pde4^UAS.cCa, Scer\GAL4^17d rescues Pde4^dnc-1

(Scheunemann et al., 2012)

Scer\GAL4^GH298, Pde4^UAS.cCa, Scer\GAL4^NP1131 rescues Pde4^dnc-1

(Scheunemann et al., 2012)

Scer\GAL4^GH298, Pde4^UAS.cCa, Scer\GAL4^c320 rescues Pde4^dnc-1

(Scheunemann et al., 2012)

Scer\GAL4^GH298, Pde4^UAS.cCa, Scer\GAL4^c305a rescues Pde4^dnc-1

(Scheunemann et al., 2012)

Scer\GAL4^GH298, Pde4^UAS.cCa, Scer\GAL4^Mef2.247 rescues Pde4^dnc-1

(Scheunemann et al., 2012)

Pde4^UAS.cCa/Scer\GAL4^Tdc2.PC rescues Pde4^ML/Pde4^dnc-M14

(Koon et al., 2011)

Pde4^UAS.cCa/Scer\GAL4^Tdc2.PC rescues Pde4^dnc-M14

(Koon et al., 2011)

Pde4^UAS.cCa/Scer\GAL4^Tdc2.PC rescues Pde4^ML

(Koon et al., 2011)

Scer\GAL4^{elav.Switch.PO}/Pde4^UAS.cCa rescues Pde4^dnc-1

(van Swinderen et al., 2009)

Pde4^UAS.cCa/Scer\GAL4^rafl-1 rescues Pde4^dnc-1

(van Swinderen et al., 2009)

Pde4^UAS.cCa/Scer\GAL4^c309 rescues Pde4^dnc-1

(van Swinderen et al., 2009)

Scer\GAL4^{elav.Switch.PO}/Pde4^UAS.cCa rescues Pde4^dnc-1

(van Swinderen, 2007)

Partially rescues

Pde4^UAS.cCa, Scer\GAL4^Mef2.247, Scer\GAL4^NP1227 partially rescues Pde4^dnc-1

(Scheunemann et al., 2012)

Pde4^UAS.cCa/Scer\GAL4^val-1 partially rescues Pde4^dnc-1

(van Swinderen et al., 2009)

Fails to rescue

Scer\GAL4^GH298/Pde4^UAS.cCa fails to rescue Pde4^dnc-1

(Scheunemann et al., 2012)

Pde4^UAS.cCa/Scer\GAL4^NP1227 fails to rescue Pde4^dnc-1

(Scheunemann et al., 2012)

Pde4^UAS.cCa/Scer\GAL4^Orco.PU fails to rescue Pde4^dnc-1

(Scheunemann et al., 2012)

Pde4^UAS.cCa/Scer\GAL4^Mef2.247 fails to rescue Pde4^dnc-1

(Scheunemann et al., 2012)

Pde4^UAS.cCa/Scer\GAL4^NP0225 fails to rescue Pde4^dnc-1

(Scheunemann et al., 2012)

Pde4^UAS.cCa/Scer\GAL4^toi-1 fails to rescue Pde4^dnc-1

(van Swinderen et al., 2009)

Pde4^UAS.cCa/Scer\GAL4^val-1 fails to rescue Pde4^dnc-1

(van Swinderen et al., 2009)

Comments

Expression of dnc^Scer\UAS.cCa under the control of Scer\GAL4^elav.PU rescues the defective short term memory performance of dnc¹/dnc¹ mutants, whether expressed throughout development or with expression restricted to adult stage with the use of a Gal80[ts] transgene.

Expression of dnc^Scer\UAS.cCa under the control of both Scer\GAL4^GH298 and Scer\GAL4^ey-OK107, both Scer\GAL4^GH298 and Scer\GAL4^17d, both Scer\GAL4^GH298 and Scer\GAL4^NP1131, both Scer\GAL4^GH298 and Scer\GAL4^c320, both Scer\GAL4^GH298 and Scer\GAL4^c305a, both Scer\GAL4^GH298 and Scer\GAL4^Mef2.247, or both Scer\GAL4^NP1227 and Scer\GAL4^Mef2.247 (but not under the control of any one of Scer\GAL4^GH298, Scer\GAL4^NP1227, Scer\GAL4^Orco.PU, Scer\GAL4^Mef2.247, or Scer\GAL4^NP0225 alone) rescues the defective short term memory performance of dnc¹/dnc¹ mutants.

Expression of dnc^Scer\UAS.cCa under the control of both Scer\GAL4^GH298 and Scer\GAL4^17d, both Scer\GAL4^GH298 and Scer\GAL4^NP1131, both Scer\GAL4^GH298 and Scer\GAL4^c305a, or both Scer\GAL4^GH298 and Scer\GAL4^Mef2.247 (but not under the control of one of Scer\GAL4^Mef2.247 or Scer\GAL4^GH298 alone) rescues the defective anaesthesia-resistant memory performance of dnc¹/dnc¹ mutants.

(Scheunemann et al., 2012)

Expression of dnc^Scer\UAS.cCa exclusively in octopaminergic neurons under the control of Scer\GAL4^Tdc2.PC rescues the increase in synaptopods at neuromuscular junction type II endings seen in dnc^M14 or dnc^ML homozygous and dnc^M14/dnc^ML heterozygous mutants.

(Koon et al., 2011)

Expression of dnc^Scer\UAS.cCa using Scer\GAL4^rafl-1 or Scer\GAL4^c309 rescues the aberrant brain responses to novelty seen in dnc¹ flies. Use of Scer\GAL4^val-1 results in partial rescue of this phenotype.

Expression of dnc^Scer\UAS.cCa using Scer\GAL4^toi-1 does not rescue the aberrant brain responses to novelty seen in dnc¹ flies.

Expression of dnc^Scer\UAS.cCa using Scer\GAL4^c309, but not Scer\GAL4^val-1 or Scer\GAL4^toi-1, rescues the defective optomotor behaviour of dnc¹ flies.

Expression of dnc^Scer\UAS.cCa induced using Scer\GAL4^{elav.Switch.PO} at a late pupal stage is sufficient to rescue the aberrant brain responses to novelty seen in dnc¹ flies.

(van Swinderen et al., 2009)

Expression of dnc^Scer\UAS.cCa under the control of Scer\GAL4^{elav.Switch.PO} (in the presence of RU486 to activate the Scer\GAL4^{elav.Switch.PO} driver) in dnc¹ flies decreases the optomotor responsiveness to wild-type levels and increases brain responsiveness to novelty to wild-type levels.

(van Swinderen, 2007)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

78785

w^*; P{UAS-Pde4.C}2/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Pde4^UAS.cCa

dnc^Scer\UAS.cCa

dnc^UAS.cCa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Cheung

(Cheung et al., 1999)

Secondary FlyBase IDs

References (20)

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