Allele Scer\GAL4^GT1

General Information
Symbol	Scer\GAL4^GT1	Species	S. cerevisiae
Name		FlyBase ID	FBal0098824
Feature type	allele	Associated gene	Scer\GAL4

Allele class
Mutagen	in vitro construct
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct (Lukacsovich and Asztalos, 1999.7.15)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AB028139 BAA78208

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\GAL4 coding region with a poly(A) signal sequence but lacking a promoter. The coding sequence is preceeded by an artificial splice acceptor site, which allows the Scer\GAL4 sequence to be transcribed as a fusion mRNA with an upstream exon sequence when the P{GT1} element is inserted into the genome. A "stop-start" sequence is placed between the splice acceptor site and the Scer\GAL4 gene which ensures that the Scer\GAL4 open reading frame is maintained in any integration event. (Lukacsovich et al., 2001) Construct: The Scer\GAL4 wild type coding sequences start at the ATG of the coding sequence, with 5' UTR removed. The coding sequence thus has no promoter. A synthetic oligonucleotide containing a "stop-start" signal has been placed immediately 5' of the coding region. This sequence has consecutive overlapping transcriptional stop and start codons with which otherwise variable reading frames can be set into the proper reading frame for Scer\GAL4. The frameshift sequence was designed to be similar to the functional kozak start site, to allow translation to initiate from the Scer\GAL4 ATG if an untranslated exon is spliced to the Scer\GAL4 mRNA. An artificial splice acceptor site was engineered to be 5' of the "stop-start" signal, and an Hsp70 terminator flanks the 3' end of the Scer\GAL4 coding region. (Lukacsovich and Asztalos, 1999.7.15)
Carried in construct	P{GT1} (Lukacsovich et al., 2001, Lukacsovich and Asztalos, 1999.7.15) PBac{GT1} (Lukacsovich et al., 2008)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 545 )
Bloomington	12404 w^*; P{GT1}BG00081 12399 w¹¹¹⁸ P{GT1}BG00040 12412 w¹¹¹⁸ P{GT1}BG00310 12415 w¹¹¹⁸ P{GT1}BG00387 12423 P{GT1}BG00541 w¹¹¹⁸ 12453 w¹¹¹⁸ P{GT1}BG01082 12468 w¹¹¹⁸ P{GT1}BG01319 12471 w¹¹¹⁸ P{GT1}BG01461 12472 w¹¹¹⁸ P{GT1}BG01463 12478 w¹¹¹⁸ P{GT1}BG00175 12494 w¹¹¹⁸ P{GT1}BG00574
Kyoto	109055 w^*; P{GT1}7-65
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Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Scer\GAL4^GT1
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Lukacsovich et al., 2008, Arch. Insect Biochem. Physiol. 69(4): 168--175 A new versatile gene-trap vector for insect transgenics. [FBrf0206814] Lukacsovich et al., 2001, Genetics 157(2): 727--742 Dual-tagging Gene Trap of novel genes in Drosophila melanogaster. [FBrf0132344]
Personal communication to FlyBase	Lukacsovich and Asztalos, 1999.7.15, Dual tagging gene-trap of novel genes in Drosophila melanogaster. Dual tagging gene-trap of novel genes in Drosophila melanogaster. [FBrf0108534]

Allele Scer\GAL4GT1

Allele Scer\GAL4^GT1