Allele Dmel\Hsc70-4^Scer\UAS.cEa

General Information
Symbol	Dmel\Hsc70-4^Scer\UAS.cEa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Elefant	FlyBase ID	FBal0098844
Feature type	allele	Associated gene	Dmel\Hsc70-4

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Elefant and Palter, 1999)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of a 2.2kb EcoRI fragment containing the Hsc70-4 coding region. (Elefant and Palter, 1999)
Carried in construct	P{UAS-Hsc70-4.WT} (Elefant and Palter, 1999, Jin et al., 2003, Chartier et al., 2006, Gruenewald et al., 2009, Hrizo and Palladino, 2010)
Cytology
Phenotypic Data
Phenotypic Class
	viable, with Scer\GAL4^how-24B (Elefant and Palter, 1999)
Phenotype Manifest In

Detailed Description
	Statement Reference Expression of Hsc70-4[Scer\UAS.cEa] under the control of Scer\GAL4[Act] is able to extend the mean life span under hyperoxic conditions by 24%. Expression of Hsc70-4[Scer\UAS.cEa] under the control of Scer\GAL4[ple.PF] rescues the degeneration of dopaminergic neurons that is seen in flies under hyperoxia. (Gruenewald et al., 2009) Flies expressing Hsc70-4^Scer\UAS.cEa under the control of Scer\GAL4^Mhc.PW show normal wing positions. (Chartier et al., 2006) Expression of Hsc70-4^Scer\UAS.cEa under the control of Scer\GAL4^337Y has no adverse effects. Embryos expressing Hsc70-4^Scer\UAS.cEa under the control of Scer\GAL4^how-24B have a wild-type muscle pattern. The majority of newly hatched or 2 day old larvae expressing Hsc70-4^Scer\UAS.cEa under the control of Scer\GAL4^156Y are able to sense, locate and burrow into a yeast paste food source within 20 minutes in a behavioural assay. (Elefant and Palter, 1999)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference Scer\GAL4^{Act5C.Switch.PR}/Hsc70-4^Scer\UAS.cEa is an enhancer \| RU486 conditional of bang sensitive phenotype of Tpi^sgk-1/Tpi^sgk-1 (Hrizo and Palladino, 2010)
Suppressor of
Statement Reference Hsc70-4^Scer\UAS.cEa, Scer\GAL4^Appl.G1a is a suppressor of increased cell death phenotype of Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^Appl.G1a (Gunawardena et al., 2003) Hsc70-4^Scer\UAS.cEa, Scer\GAL4^Appl.G1a is a suppressor of increased cell death phenotype of Scer\GAL4^Appl.G1a, Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} (Gunawardena et al., 2003) Hsc70-4^Scer\UAS.cEa, Scer\GAL4^Mhc.PW is a suppressor of visible phenotype of Hsap\PABPN1^{17ala.Scer\UAS}, Scer\GAL4^Mhc.PW (Chartier et al., 2006)
Phenotype Manifest In
Enhancer of
Statement Reference Hsc70-4^Scer\UAS.cEa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Avic\GFP^{Fmr1.CGG90.E.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Jin et al., 2003)
Suppressor of
Statement Reference Hsc70-4^Scer\UAS.cEa, Scer\GAL4^179Y is a suppressor of axon phenotype of Hsap\ATXN3^{Q77.Scer\UAS.T:Ivir\HA1,T:HIV-1\nes}, Scer\GAL4^179Y (Gunawardena et al., 2003) Hsc70-4^Scer\UAS.cEa, Scer\GAL4^Appl.G1a is a suppressor of axon phenotype of Hsap\ATXN3^{Q77.Scer\UAS.T:Ivir\HA1,T:HIV-1\nes}, Scer\GAL4^Appl.G1a (Gunawardena et al., 2003) Hsc70-4^Scer\UAS.cEa, Scer\GAL4^Appl.G1a is a suppressor of neuron phenotype of Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^Appl.G1a (Gunawardena et al., 2003) Hsc70-4^Scer\UAS.cEa, Scer\GAL4^Appl.G1a is a suppressor of neuron phenotype of Scer\GAL4^Appl.G1a, Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} (Gunawardena et al., 2003) Hsc70-4^Scer\UAS.cEa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a suppressor \| partially of eye phenotype of Avic\GFP^{Fmr1.CGG90.E.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Jin et al., 2003) Hsc70-4^Scer\UAS.cEa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a suppressor \| partially of ommatidium phenotype of Avic\GFP^{Fmr1.CGG90.E.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Jin et al., 2003) Hsc70-4^Scer\UAS.cEa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a suppressor \| partially of pigment cell phenotype of Avic\GFP^{Fmr1.CGG90.E.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Jin et al., 2003) Hsc70-4^Scer\UAS.cEa, Scer\GAL4^Mhc.PW is a suppressor of wing phenotype of Hsap\PABPN1^{17ala.Scer\UAS}, Scer\GAL4^Mhc.PW (Chartier et al., 2006)
Additional Comments
Genetic Interactions
Statement Reference The mechanical stress sensitivity of Tpi[sgk-1] animals is enhanced if they are also expressing Hsc70-4[Scer\UAS.cEa] under the control of Scer\GAL4[Act5C.Switch.PR] (in the presence of Mifepristone). (Hrizo and Palladino, 2010)
Xenogenetic Interactions
Statement Reference Coexpression of the Hsc70-4^Scer\UAS.cEa transgene results in efficient suppression of the wing posture phenotype of Scer\GAL4^Mhc.PW>Hsap\PABPN1^{17ala.Scer\UAS} flies. (Chartier et al., 2006)
Complementation & Rescue Data
Comments
Stocks ( 2 )
Bloomington	5846 w¹²⁶; P{UAS-Hsc70-4.WT}B
Kyoto	108464 w¹²⁶; P{UAS-Hsc70-4.WT}B
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Hsc70-4^Scer\UAS.cEa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Elefant (Elefant and Palter, 1999)
Secondary FlyBase IDs

References ( 6 )
Research paper	Hrizo and Palladino, 2010, Neurobiol. Disease 40(3): 676--683 Hsp70- and Hsp90-mediated proteasomal degradation underlies TPI(sugarkill) pathogenesis in Drosophila. [FBrf0212050] Gruenewald et al., 2009, Free Radical Biol. Med. 46(12): 1668--1676 Hyperoxia-induced neurodegeneration as a tool to identify neuroprotective genes in Drosophila melanogaster. [FBrf0208024] Chartier et al., 2006, EMBO J. 25(10): 2253--2262 A Drosophila model of oculopharyngeal muscular dystrophy reveals intrinsic toxicity of PABPN1. [FBrf0190356] Gunawardena et al., 2003, Neuron 40(1): 25--40 Disruption of axonal transport by loss of huntingtin or expression of pathogenic polyQ proteins in Drosophila. [FBrf0167960] Jin et al., 2003, Neuron 39(5): 739--747 RNA-mediated neurodegeneration caused by the Fragile X premutation rCGG repeats in Drosophila. [FBrf0162265] Elefant and Palter, 1999, Mol. Biol. Cell 10(7): 2101--2117 Tissue-specific expression of dominant negative mutant Drosophila HSC70 causes developmental defects and lethality. [FBrf0109657]

Allele Dmel\Hsc70-4Scer\UAS.cEa

Allele Dmel\Hsc70-4^Scer\UAS.cEa