FlyBase Allele Report: Dmel\Hsc70-4[UAS.cEa]

General Information

Symbol

Dmel\Hsc70-4^UAS.cEa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Elefant

FlyBase ID

FBal0098844

Feature type

allele

Associated gene

Dmel\Hsc70-4

Associated Insertion(s)

Carried in Construct

P{UAS-Hsc70-4.WT}

Also Known As

UAS-Hsc70-4, UAS-Hsc70-4.WT, UAS-Hsc4

Key Links

Transgenic product class

wild_type

(Elefant and Palter, 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Elefant and Palter, 1999)

Mutagen

in vitro construct

(Elefant and Palter, 1999)

Progenitor genotype

Carried in construct

P{UAS-Hsc70-4.WT}

Cytology

Description

UASt regulatory sequences drive expression of a 2.2kb EcoRI fragment containing the Hsc70-4 coding region.

(Elefant and Palter, 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Hsc70-4

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates neurodegenerative disease

modeled by Zzzz\CAG^{127Q.UAS.Tag:HA}

(Gunawardena et al., 2003)

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Gunawardena et al., 2003)

modeled by Hsap\ATXN3^{Q77.UAS.Tag:HA,Tag:NES(HIV-1-Rev)}

(Gunawardena et al., 2003)

modeled by Zzzz\CGG^90.UAS.EGFP

(Jin et al., 2003)

ameliorates erythrokeratodermia variabilis

modeled by Hsap\GJB3^{F137L.UAS.Tag:MYC}

(Tang et al., 2015)

ameliorates fragile X-associated tremor/ataxia syndrome

modeled by Zzzz\CGG^90.UAS.EGFP

(Oh et al., 2015)

ameliorates obesity

modeled by HDAC6^KO

(Yan et al., 2017)

modeled by Lsd-2^UAS.EYFP

(Yan et al., 2017)

ameliorates amyotrophic lateral sclerosis type 10

modeled by Hsap\TARDBP^UAS.cEa

(Berson et al., 2017)

exacerbates Huntington's disease

modeled by Zzzz\CAG^{127Q.UAS.Tag:HA}

(Rai and Tapadia, 2022)

modeled by Hsap\HTT^Q93.ex1.UAS

(Rai and Tapadia, 2022)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable, with Scer\GAL4^how-24B

(Elefant and Palter, 1999)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Hsc70-4^Scer\UAS.cEa under the control of Scer\GAL4^Act is able to extend the mean life span under hyperoxic conditions by 24%.

Expression of Hsc70-4^Scer\UAS.cEa under the control of Scer\GAL4^ple.PF rescues the degeneration of dopaminergic neurons that is seen in flies under hyperoxia.

(Gruenewald et al., 2009)

Flies expressing Hsc70-4^Scer\UAS.cEa under the control of Scer\GAL4^Mhc.PW show normal wing positions.

(Chartier et al., 2006)

Expression of Hsc70-4^Scer\UAS.cEa under the control of Scer\GAL4^337Y has no adverse effects. Embryos expressing Hsc70-4^Scer\UAS.cEa under the control of Scer\GAL4^how-24B have a wild-type muscle pattern. The majority of newly hatched or 2 day old larvae expressing Hsc70-4^Scer\UAS.cEa under the control of Scer\GAL4^156Y are able to sense, locate and burrow into a yeast paste food source within 20 minutes in a behavioural assay.

(Elefant and Palter, 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Scer\GAL4^{Act5C.Switch.PR}/Hsc70-4^UAS.cEa is an enhancer of bang sensitive phenotype of Tpi^sgk-1

(Hrizo and Palladino, 2010)

NOT Enhancer of

Statement

Reference

Hsc70-4^UAS.cEa, Scer\GAL4^GMR.PU is a non-enhancer of visible | adult stage | progressive phenotype of Hsap\ATXN3^{fl.Q78.UAS.Tag:MYC}, Scer\GAL4^GMR.PU

(Vinatier et al., 2015)

Hsc70-4^UAS.cEa, Scer\GAL4^GMR.PU is a non-enhancer of abnormal eye color | progressive phenotype of Hsap\ATXN3^{fl.Q78.UAS.Tag:MYC}, Scer\GAL4^GMR.PU

(Vinatier et al., 2015)

Suppressor of

Statement

Reference

Hsc70-4^UAS.cEa, Scer\GAL4^Mef2.PU is a suppressor | partially of abnormal body size | P-stage phenotype of NdufA3^GD4003, Scer\GAL4^Mef2.PU

(Pueyo et al., 2023)

Hsc70-4^UAS.cEa, Scer\GAL4^GMR.long is a suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.long, Zzzz\CGG^{100.5'UTR.UAS.GFP}

(Oh et al., 2015)

Hsc70-4^UAS.cEa, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Hsap\GJB3^{F137L.UAS.Tag:MYC}, Scer\GAL4^GMR.PF

(Tang et al., 2015)

Hsc70-4^UAS.cEa, Scer\GAL4^GMR.PU is a suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.PU, Zzzz\CGG^90.UAS.EGFP

(Qurashi et al., 2011)

Hsc70-4^UAS.cEa, Scer\GAL4^Mhc.PW is a suppressor of visible phenotype of Hsap\PABPN1^17ala.UAS, Scer\GAL4^Mhc.PW

(Chartier et al., 2006)

Hsc70-4^UAS.cEa, Scer\GAL4^Appl.G1a is a suppressor of increased cell death phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^Appl.G1a

(Gunawardena et al., 2003)

Hsc70-4^UAS.cEa, Scer\GAL4^Appl.G1a is a suppressor of increased cell death phenotype of Scer\GAL4^Appl.G1a, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Gunawardena et al., 2003)

NOT Suppressor of

Statement

Reference

Hsc70-4^UAS.cEa, Scer\GAL4^GMR.PU is a non-suppressor of visible | adult stage | progressive phenotype of Hsap\ATXN3^{fl.Q78.UAS.Tag:MYC}, Scer\GAL4^GMR.PU

(Vinatier et al., 2015)

Hsc70-4^UAS.cEa, Scer\GAL4^GMR.PU is a non-suppressor of abnormal eye color | progressive phenotype of Hsap\ATXN3^{fl.Q78.UAS.Tag:MYC}, Scer\GAL4^GMR.PU

(Vinatier et al., 2015)

Hsc70-4^UAS.cEa, Scer\GAL4^GMR.PU is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Kuo et al., 2013)

Hsc70-4^UAS.cEa, Scer\GAL4^GMR.PU is a non-suppressor of visible | adult stage phenotype of DnaJ-1^UAS.cKb, Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Kuo et al., 2013)

Phenotype Manifest In

Enhancer of

Statement

Reference

Hsc70-4^UAS.cEa, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, Zzzz\CGG^90.UAS.EGFP

(Jin et al., 2003)

NOT Enhancer of

Statement

Reference

Hsc70-4^UAS.cEa, Scer\GAL4^GMR.PU is a non-enhancer of eye | progressive phenotype of Hsap\ATXN3^{fl.Q78.UAS.Tag:MYC}, Scer\GAL4^GMR.PU

(Vinatier et al., 2015)

Suppressor of

Statement

Reference

Hsc70-4^UAS.cEa, Scer\GAL4^Mef2.PU is a suppressor | partially of sarcomere | larval stage phenotype of NdufA3^GD4003, Scer\GAL4^Mef2.PU

(Pueyo et al., 2023)

Hsc70-4^UAS.cEa, Scer\GAL4^Mef2.PU is a suppressor | partially of larval somatic muscle | third instar larval stage phenotype of NdufA3^GD4003, Scer\GAL4^Mef2.PU

(Pueyo et al., 2023)

Scer\GAL4^Mhc.PK/Hsc70-4^UAS.cEa is a suppressor of mesothoracic tergotrochanter muscle cell | progressive phenotype of HDAC6^KO

(Yan et al., 2017)

Scer\GAL4^Mhc.PK/Hsc70-4^UAS.cEa is a suppressor of lipid droplet | adult stage | progressive phenotype of HDAC6^KO

(Yan et al., 2017)

Hsc70-4^UAS.cEa is a suppressor | partially of mesothoracic tergotrochanter muscle cell phenotype of Lsd-2^UAS.cUa, Scer\GAL4^Mhc.PK

(Yan et al., 2017)

Hsc70-4^UAS.cEa is a suppressor | partially of lipid droplet | adult stage phenotype of Lsd-2^UAS.cUa, Scer\GAL4^Mhc.PK

(Yan et al., 2017)

Hsc70-4^UAS.cEa, Scer\GAL4^GMR.long is a suppressor of eye phenotype of Scer\GAL4^GMR.long, Zzzz\CGG^{100.5'UTR.UAS.GFP}

(Oh et al., 2015)

Hsc70-4^UAS.cEa, Scer\GAL4^GMR.long is a suppressor of ommatidium phenotype of Scer\GAL4^GMR.long, Zzzz\CGG^{100.5'UTR.UAS.GFP}

(Oh et al., 2015)

Hsc70-4^UAS.cEa, Scer\GAL4^GMR.PF is a suppressor of ommatidium phenotype of Hsap\GJB3^{F137L.UAS.Tag:MYC}, Scer\GAL4^GMR.PF

(Tang et al., 2015)

Hsc70-4^UAS.cEa, Scer\GAL4^GMR.PU is a suppressor of eye phenotype of Scer\GAL4^GMR.PU, Zzzz\CGG^90.UAS.EGFP

(Qurashi et al., 2011)

Hsc70-4^UAS.cEa, Scer\GAL4^GMR.PU is a suppressor of ommatidium | adult stage phenotype of Scer\GAL4^GMR.PU, Zzzz\CGG^90.UAS.EGFP

(Qurashi et al., 2011)

Hsc70-4^UAS.cEa, Scer\GAL4^GMR.PU is a suppressor of interommatidial bristle | adult stage phenotype of Scer\GAL4^GMR.PU, Zzzz\CGG^90.UAS.EGFP

(Qurashi et al., 2011)

Hsc70-4^UAS.cEa, Scer\GAL4^Mhc.PW is a suppressor of wing phenotype of Hsap\PABPN1^17ala.UAS, Scer\GAL4^Mhc.PW

(Chartier et al., 2006)

Hsc70-4^UAS.cEa, Scer\GAL4^179Y is a suppressor of axon phenotype of Hsap\ATXN3^{Q77.UAS.Tag:HA,Tag:NES(HIV-1-Rev)}, Scer\GAL4^179Y

(Gunawardena et al., 2003)

Hsc70-4^UAS.cEa, Scer\GAL4^Appl.G1a is a suppressor of axon phenotype of Hsap\ATXN3^{Q77.UAS.Tag:HA,Tag:NES(HIV-1-Rev)}, Scer\GAL4^Appl.G1a

(Gunawardena et al., 2003)

Hsc70-4^UAS.cEa, Scer\GAL4^Appl.G1a is a suppressor of neuron phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^Appl.G1a

(Gunawardena et al., 2003)

Hsc70-4^UAS.cEa, Scer\GAL4^Appl.G1a is a suppressor of neuron phenotype of Scer\GAL4^Appl.G1a, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Gunawardena et al., 2003)

Hsc70-4^UAS.cEa, Scer\GAL4^GMR.PF is a suppressor of ommatidium phenotype of Scer\GAL4^GMR.PF, Zzzz\CGG^90.UAS.EGFP

(Jin et al., 2003)

Hsc70-4^UAS.cEa, Scer\GAL4^GMR.PF is a suppressor of pigment cell phenotype of Scer\GAL4^GMR.PF, Zzzz\CGG^90.UAS.EGFP

(Jin et al., 2003)

Hsc70-4^UAS.cEa, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, Zzzz\CGG^90.UAS.EGFP

(Jin et al., 2003)

NOT Suppressor of

Statement

Reference

Hsc70-4^UAS.cEa, Scer\GAL4^GMR.PU is a non-suppressor of eye | progressive phenotype of Hsap\ATXN3^{fl.Q78.UAS.Tag:MYC}, Scer\GAL4^GMR.PU

(Vinatier et al., 2015)

Hsc70-4^UAS.cEa, Scer\GAL4^GMR.PU is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Kuo et al., 2013)

Hsc70-4^UAS.cEa, Scer\GAL4^GMR.PU is a non-suppressor of eye phenotype of DnaJ-1^UAS.cKb, Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Kuo et al., 2013)

Hsc70-4^UAS.cEa, Scer\GAL4^GMR.PU is a non-suppressor of photoreceptor phenotype of DnaJ-1^UAS.cKb, Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Kuo et al., 2013)

Other

Statement

Reference

HDAC6^UAS.cDa, Hsc70-4^UAS.cEa, Scer\GAL4^Mhc.PK has mesothoracic tergotrochanter muscle cell phenotype

(Yan et al., 2017)

HDAC6^UAS.cDa, Hsc70-4^UAS.cEa, Scer\GAL4^Mhc.PK has lipid droplet | adult stage phenotype

(Yan et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The mechanical stress sensitivity of Tpi^sgk-1 animals is enhanced if they are also expressing Hsc70-4^Scer\UAS.cEa under the control of Scer\GAL4^{Act5C.Switch.PR} (in the presence of Mifepristone).

(Hrizo and Palladino, 2010)

Xenogenetic Interactions

Statement

Reference

The eye degeneration phenotype as well as formation of inclusions in the ommatidia observed in adult flies expressing Zzzz\CGG^{100.5'UTR.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^GMR.long (and maintained at 28[o]C) is significantly suppressed by co-expression of Hsc70-4^Scer\UAS.cEa

(Oh et al., 2015)

Co-expression of Hsc70-4^Scer\UAS.cEa suppresses the ommatidial degeneration and loss of pigmentation induced by Scer\GAL4^GMR.PF>Hsap\GJB3^{F137L.Scer\UAS.T:Hsap\MYC} in more than 90% of flies.

(Tang et al., 2015)

Expression of Hsc70-4^Scer\UAS.cEa does not suppress the external eye degeneration seen when Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} is expressed under the control of Scer\GAL4^GMR.PU.

Expression of Hsc70-4^Scer\UAS.cEa does not suppress the external eye degeneration phenotype seen when Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} and DnaJ-1^Scer\UAS.cKb are co-expressed under the control of Scer\GAL4^GMR.PU.

(Kuo et al., 2013)

The eye degeneration phenotype (rough eye with disorganized ommatidial lattice and interommatidial bristles) characteristic for flies expressing Zzzz\CGG^{90.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^GMR.PU is partially rescued by co-expression of Hsc70-4^Scer\UAS.cEa.

(Qurashi et al., 2011)

Coexpression of the Hsc70-4^Scer\UAS.cEa transgene results in efficient suppression of the wing posture phenotype of Scer\GAL4^Mhc.PW>Hsap\PABPN1^{17ala.Scer\UAS} flies.

(Chartier et al., 2006)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

5846

w¹²⁶; P{UAS-Hsc70-4.WT}B

Kyoto

108464