Open Close
General Information
Symbol
Dmel\poloS025604
Species
D. melanogaster
Name
FlyBase ID
FBal0100127
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
polo9
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

P{lacW} insertion into the non-coding region of the first exon, affecting both of the polo transcription units.

Insertion components
P{lacW}poloS025604
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

poloS025604 heterozygotes are viable.

poloS025604 homozygous third instar larval brain exhibit higher mitotic index than controls.

poloΔUSE.GFP expressing poloS025604 homozygous adults exhibit malformation of the abdominal epidermis and tergite defects compared to controls.

poloΔUSE.GFP expressing poloS025604 homozygous third instar larval mitotic neuroblasts exhibit an abnormal number of chromosomes compared to controls.

Homozygous embryos show localised increased or decreased in cardial cell (CC) number, larger than normal CC nuclei and incorrectly positioned CCs.

Approximately 90% of polo1/poloS025604 germaria have a 'two-oocyte phenotype' in region 3 (a single oocyte is already determined in approximately 70% of wild-type germaria by this stage).

Analysis of the few polopA2.T:Avic\GFP;poloS025604 escapers indicates that these individuals exhibit strong defects in abdominal morphology, with the complete absence or incorrect formation of tergites, while all other adult structures appear normal.

Only large polytene larval cells are observed in the epidermis of polopA2.T:Avic\GFP;poloS025604/poloS025604 pupa. No histoblasts are detected in polopA2.T:Avic\GFP;poloS025604/poloS025604 pupa, but are present in larvae.

Transgenic polopA1.T:Avic\GFP;poloS025604 adult flies exhibit a mild abdominal phenotype.

Several nuclei in prophase have one dislocated centrosome in syncytial embryos from heterozygous poloS025604 mutant mothers.

Mutant larval central brains have a normal number of neuroblasts at 24 hours after larval hatching (ALH) (36.5 +/- 4.5). However, by 96 hours ALH, the number of neuroblasts in the mutant central brains is increased dramatically to 253.6 +/- 44.5 (compared to 90.7 +/- 7.0 in wild type).

Clones in the central larval brain derived from single cell mutant neuroblasts show an ectopic self-renewal phenotype, containing multiple neuroblast cells (wild-type single cell clones only contain one neuroblast).

The tight coupling of spindle orientation with crescent formation seen in wild-type neuroblasts is disrupted in mutant metaphase neuroblasts with two centrosomes.

Meiosis does not initiate properly in region 2a of the polo1/poloS025604 germarium. Cysts often contain abnormal synaptonemal complexes and meiosis restriction to one cell is delayed.

poloS025604/poloS132408 animals survive to adult but are sterile.

Lethal phase is during the third larval instar. Mutant larval brains have a mitotic index that is elevated about 11-fold above that of wild-type cells. The mitotic chromosomes are much more highly condensed than normal. Many cells appear arrested in a metaphase-like state (the metaphase/anaphase ratio is 51.4 compared to 5.8 in wild-type cells). The proportion of figures in which chromosomes are well separated at anaphase is relatively low, although in absolute numbers it is comparable to that in wild-type cells. 16.1% of mitotic cells are aneuploid or polyploid. A small fraction (1.4%) of circular mitotic figures is seen. Some cells show premature separation of at least one set of their sister chromatids. The metaphase arrested cells have robust arrays of microtubules but lack asters at their poles. 79.3% of pairs of sister centromeric regions show separation.

Lethality acts in the prepupal and pupal stages.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
NOT suppressed by
Enhancer of
Statement
Reference
Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Enhanced by
Statement
Reference

poloS025604 has aster & neuroblast phenotype, enhanceable by Cdc27S092309

poloS025604 has spindle & neuroblast phenotype, enhanceable by Cdc27S092309

Suppressed by
Statement
Reference

poloS025604/polo1 has germarium region 3 phenotype, suppressible by Sirt117/Sir2[+]

poloS025604 has neuroblast & larval brain | somatic clone phenotype, suppressible by Scer\GAL4wor.PA/numbPTB.UAS.GFP

NOT suppressed by
Statement
Reference
Enhancer of
Statement
Reference

poloS025604 is an enhancer of aster & neuroblast phenotype of Cdc27S092309

poloS025604 is an enhancer of spindle & neuroblast phenotype of Cdc27S092309

Other
Additional Comments
Genetic Interactions
Statement
Reference

poloS025604/+ ; Df(3R)Exel6157/+ double heterozygous embryos show asymmetric cell division defects in "svp" cardiac progenitor cells that are significantly more severe than the additive effects of each of the two single heterozygotes. Defects in the symmetric cell divisions that give rise to the tin-expressing cardial cells are also significantly more severe in the double heterozygotes.

poloS025604/+ ; Df(1)CHES-1-like1/+ double heterozygous embryos show asymmetric cell division defects in "svp" cardiac progenitor cells that are significantly more severe than the additive effects of each of the two single heterozygotes. Defects in the symmetric cell divisions that give rise to the tin-expressing cardial cells are also significantly more severe in the double heterozygotes.

Expression of maelS138D.Scer\UAS.P\T.T:Zzzz\FLAG under the control of Scer\GAL4nos.PG partially suppresses the defect in oocyte commitment (high frequency of 'two-oocyte phenotype' in region 3 of the germarium) that is seen in polo1/poloS025604 germaria.

Expression of maelS138A.Scer\UAS.P\T.T:Zzzz\FLAG under the control of Scer\GAL4nos.PG does not suppress the defect in oocyte commitment (high frequency of 'two-oocyte phenotype' in region 3 of the germarium) that is seen in polo1/poloS025604 germaria.

Sir217/+ suppresses the defect in oocyte commitment (high frequency of 'two-oocyte phenotype' in region 3 of the germarium) that is seen in polo1/poloS025604 germaria.

pch2EY01788a/pch2EY01788a suppresses the defect in oocyte commitment (high frequency of 'two-oocyte phenotype' in region 3 of the germarium) that is seen in polo1/poloS025604 germaria.

The ectopic neuroblast self-renewal phenotype seen in single cell poloS025604 clones in the larval central brain is suppressed by expression of numbPTB.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4wor.PA.

The non-disjunction phenotype seen in mei-S3328/+ sperm is partially suppressed by polo1, poloS025604.

The lethal phase of Cdc27S092309 is advanced to an earlier developmental stage when in combination with poloS025604. The stage of death is also earlier than with poloS025604 alone.

Xenogenetic Interactions
Statement
Reference

Expression of Mmus\Plk1Scer\UAS.P\T.T:Avic\GFP under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 does not rescue the sterility of polo1/poloS025604 females.

The majority of poloS025604 homozygotes expressing Mmus\Plk1Scer\UAS.P\T.T:Avic\GFP under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 only survive to the second larval instar stage, in contrast to poloS025604 single homozygotes which survive to the third larval instar stage.

Complementation and Rescue Data
Not rescued by
Comments

Nearly all polopA2.T:Avic\GFP;poloS025604 flies die during pupariation.

Transgenic poloT:Avic\GFP;poloS025604 adult flies exhibit wild-type morphology.

Transgenic polopA1.T:Avic\GFP;poloS025604 adult flies exhibit a mild abdominal phenotype.

poloT:Avic\GFP rescues the sterility of polo1/poloS025604 females.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (7)
References (18)