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General Information
Symbol
Dmel\Invadolysin4Y7
Species
D. melanogaster
Name
FlyBase ID
FBal0100235
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Imprecise hopping of P{PZ}tws04017 generates an Invadolysin mutant.
P-element insertion 40 bp upstream of the start of transcription.
Insertion components
P{PZ}Invadolysin4Y7
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Examination of the fat body in Invadolysin4Y7 third instar larvae reveals a dramatic reduction in thickness and a significant reduction in the ratio of triglyceride-to-protein content. Overall mitochondrial number is not affected in these mutants. Staining with Mitotracker suggests functional defects in these mitochondria. These mitochondria also exhibit alterations in diameter and length compared to controls. Invadolysin4Y7 mutant third-instar larvae display an ATP level only 40% that of wild-type. When fed on normal food, approximately 80% of Invadolysin4Y7 mutant larvae die after 5 days (prior to pupation), whereas 20% attempt pupariation. When fed Compound C, Invadolysin4Y7 larvae live for up to 7 days and attempt pupation. Although the pupae are abnormal and unable to complete metamorphosis (as they lack imaginal discs), this represents a greater longevity than on control food. Invadolysin4Y7 mutant larvae fed AICAR show no discernible change in development (with larvae dying prior to pupation and only 10% attempting pupariation). Invadolysin4Y7 mutant third-instar larvae display a defective response to hypoxia, appearing unresponsive to a hypoxic environment.
l(3)IX-144Y7 mutant third instar larvae have a much-reduced brain size and missing imaginal discs. l(3)IX-144Y7 mutant neuroblasts from third instar larval brains have mitotic chromosomes that are hypercondensed length-wise but appear loosely compacted with a ragged periphery. This phenotype is not rescued by allowing more time in mitosis with colchicine treatment. l(3)IX-144Y7 mutant polytene chromosomes from third instar larval salivary glands lack their distinctive banding, their chromosome arms appear twisted and frayed, and they are approximately half the size of wild type. It is difficult to identify an obvious chromocentre in many l(3)IX-144Y7 mutant polytene nuclei. BrdU incorporation is clearly reduced in l(3)IX-144Y7 mutant neuroblasts from third instar larval brains. Larval neuroblasts of l(3)IX-144Y7 mutants exhibit abnormal spindle morphology, with only 2% of mitotic cells having a normal bipolar spindle, 37% having a monopolar spindle, 34% having a disorganised spindle, and 27% having a bipolar asymmetric spindle. Mutant spindles also appear to have thicker bundles of microtubules compared to wild type. Almost no anaphase mitotic figures are observed in mutant neuroblasts, consistent with a metaphase delay or arrest. Nearly 70% of mitotic figures in l(3)IX-144Y7 mutant neuroblasts appear to have only one focus of centrosomal staining, often having a dumbbell shape suggesting that centrosomes have duplicated but not separated. Germ cells fail to migrate and coalesce in l(3)IX-144Y7 mutant embryos and mutant larvae lack gonads.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
The rough eye seen upon expression of InvadolysinScer\UAS.N.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF at 29[o]C is suppressed in a Invadolysin4Y7 mutant background.
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments
Precise excision of the l(3)IX-144Y7 P-element reverts the mutant phenotype and restores viability.
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (3)