FlyBase Allele Report: Dmel\ctp[G0371]

General Information

Symbol

Dmel\ctp^G0371

Species

D. melanogaster

Name

FlyBase ID

FBal0100301

Feature type

allele

Associated gene

Dmel\ctp

Associated Insertion(s)

P{lacW}ctp^G0371

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Schäfer et al., 1999.8.12)

Progenitor genotype

Associated Insertion(s)

P{lacW}ctp^G0371

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

X:4,688,535..4,688,651 [+]

(Kreko-Pierce and Eaton, 2017, Batlevi et al., 2010, Peter et al., 2002, Schäfer et al., 1999.8.12)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

AJ426687

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Kennedy's disease

modeled by Hsap\AR^Q52.UAS

(Batlevi et al., 2010)

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

Hsap\AR^Q52.Scer\UAS disease model requires Hsap\AR ligand testosterone. Larvae raised in the presence of dihydroxytestosterone.

(Batlevi et al., 2010)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | third instar larval stage

(Batlevi et al., 2010)

lethal | pharate adult stage

(Peter et al., 2002)

lethal | pupal stage

(Peter et al., 2002)

lethal | recessive

(Schäfer et al., 1999.8.12)

lethal - all die before end of pupal stage

(Batlevi et al., 2010)

some die during pupal stage

(Batlevi et al., 2010)

Phenotype Manifest In

lysosome | pupal stage

(Batlevi et al., 2010)

NMJ bouton | third instar larval stage

(Batlevi et al., 2010)

salivary gland | pupal stage

(Batlevi et al., 2010)

Detailed Description

Statement

Reference

Head eversion occurs at 15 hours after puparium formation in ctp^G0371 mutant pupae. 12 hours after head eversion, a defect in salivary gland death is evident. All pupae fail to complete salivary gland degradation, compared to control animals which completely lack salivary glands at this stage. 39% of mutant pupae have intact glands, and 61% display partially fragmented salivary glands.

Autophagy is defective in ctp^G0371 mutant salivary glands, and the number of autophagosomes present in salivary gland cells is reduced compared to controls. ctp^G0371 mutant salivary gland cells contain protein inclusions.

ctp^G0371 mutant third instar larvae display motility defects. ctp^G0371 mutants possess decreased neuromuscular junction bouton numbers compared to controls, and protein inclusions are present in larval muscles.

(Batlevi et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

ctp^G0371 is an enhancer of abnormal locomotor behavior | conditional phenotype of Hsap\AR^Q52.UAS, Scer\GAL4^Toll-6-D42

(Batlevi et al., 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

ctp^G0371 has salivary gland | pupal stage phenotype, enhanceable by Scer\GAL4^da.G32/BacA\p35^UAS.cHa

(Batlevi et al., 2010)

Suppressed by

Statement

Reference

ctp^G0371 has salivary gland | pupal stage phenotype, suppressible by Scer\GAL4^fkh.PH/Atg1^GS10797

(Batlevi et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Atg1^GS10797 in the salivary glands under the control of Scer\GAL4^fkh.PH in ctp^G0371 mutant animals eliminates the accumulation of protein inclusions in salivary gland cells.

(Batlevi et al., 2010)

Xenogenetic Interactions

Statement

Reference

Expression of BacA\p35^Scer\UAS.cHa under the control of Scer\GAL4^da.G32 in ctp^G0371 mutant animals results in almost complete inhibition of salivary gland degradation, and 96% of animals display intact salivary glands.

Larval motility defects observed in animals expressing Hsap\AR^Q52.Scer\UAS in motor neurons under the control of Scer\GAL4^D42 and fed on food supplemented with DHT, is enhanced in a ctp^G0371 mutant background.

(Batlevi et al., 2010)

Complementation and Rescue Data

Rescued by

ctp^G0371 is rescued by Scer\GAL4^fkh.PH/ctp^UAS.cBa

(Batlevi et al., 2010)

Partially rescued by

ctp^G0371 is partially rescued by ctp^UAS.cBa/Scer\GAL4^da.G32

(Batlevi et al., 2010)

Comments

Expression of ctp^Scer\UAS.cBa under the control of Scer\GAL4^fkh.PH in ctp^G0371 mutant animals rescues the defects in salivary gland degradation. Most animals have no salivary glands, with a small number showing fragmented salivary glands.

Expression of ctp^Scer\UAS.cBa throughout development under the control of Scer\GAL4^da.G32 rescues the viability of 63% of ctp^G0371 adult males.

(Batlevi et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

11988

w^67c23 P{lacW}ctp^G0371/FM7c

Kyoto

111880

w^67c23 P{lacW}ctp^G0371 P{neoFRT}19A/FM7c; P{ey-FLP.N}5