Head eversion occurs at 15 hours after puparium formation in ctpG0371
mutant pupae. 12 hours after head eversion, a defect in salivary gland death is evident. All pupae fail to complete salivary gland degradation, compared to control animals which completely lack salivary glands at this stage. 39% of mutant pupae have intact glands, and 61% display partially fragmented salivary glands.
Autophagy is defective in ctpG0371
mutant salivary glands, and the number of autophagosomes present in salivary gland cells is reduced compared to controls. ctpG0371
mutant salivary gland cells contain protein inclusions.
mutant third instar larvae display motility defects. ctpG0371
mutants possess decreased neuromuscular junction bouton numbers compared to controls, and protein inclusions are present in larval muscles.