Allele Dmel\cnn^E2

General Information
Symbol	Dmel\cnn^E2	Species	D. melanogaster
Name		FlyBase ID	FBal0100377
Feature type	allele	Associated gene	Dmel\cnn

Map ( GBrowse )
Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Vaizel-Ohayon and Schejter, 1999)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 2R:9,329,751..9,329,751 evidence=experimental na_change=C9329751T pr_change=Q388\|cnn-PD,Q618\|cnn-PC,Q418\|cnn-PB,Q446\|c nn-PA,Q428@\|cnn-PE reported_na_change=C?T reported_pr_change=Q446@ (Vaizel-Ohayon and Schejter, 1999)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: Q446@. Nucleotide substitution: CAG codon is replaced by a UAG codon. Contains an stop codon within at codon 446, in the N terminal of the coding region. (Vaizel-Ohayon and Schejter, 1999)
Cytology
Phenotypic Data
Phenotypic Class
	female sterile (Vaizel-Ohayon and Schejter, 1999) male sterile (Vaizel-Ohayon and Schejter, 1999) viable (Vaizel-Ohayon and Schejter, 1999)
Phenotype Manifest In
	centrosome \| embryonic stage 4 (Vaizel-Ohayon and Schejter, 1999) embryo (Vaizel-Ohayon and Schejter, 1999) embryonic cortex & actin filament (Vaizel-Ohayon and Schejter, 1999) pericentriolar material \| embryonic stage 4 (Vaizel-Ohayon and Schejter, 1999) spindle \| embryonic stage 4 (Vaizel-Ohayon and Schejter, 1999)
Detailed Description
	Statement Reference Syncitial embryos from cnn^E2/cnn^HK21 mothers show a lack of spindle pole localisation of centrosome associated antigens Cp190 and γTub23C in a variable manner, suggesting that the Microtubule Organising Centres are at least structurally and functionally impaired, or possibly missing altogether. Embryos from cnn^E2/cnn^HK21 mothers exhibit an abnormal and disorganised spatial arrangement of nuclei during syncitial divisions, becoming increasingly severe during later divisions. This leads to a cessation of development before formation of a cellular blastoderm. In contrast to wild-type, embryos from cnn^HK21 mothers show an unstructured cortical microfilament organisation during syncitial development. Syncitial embryos from cnn^E2/cnn^HK21 mothers, show abnormally shaped mitotic spindles that are characteristically squat with ill-defined poles. During interphase and prophase, a variable number (as opposed to strictly two in wild type) of spread out and less discrete astral microtubule-like structures are seen, despite having no γTub23C localising nucleating core. (Vaizel-Ohayon and Schejter, 1999)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	cnn^E2
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Vaizel-Ohayon and Schejter, 1999, Curr. Biol. 9(16): 889--898 Mutations in centrosomin reveal requirements for centrosomal function during early Drosophila embryogenesis. [FBrf0111509]

Allele Dmel\cnnE2

Allele Dmel\cnn^E2