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General Information
Symbol
Dmel\Syt4UAS.cLa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Littleton
FlyBase ID
FBal0100393
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Syt4
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

A 3.1kb fragment encompassing the Syt4 ORF is driven by UASt sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of SytIVScer\UAS.cLa under the control of Scer\GAL4elav.PLu in a wild-type background has no effect on excitatory junctional potential (EJP) amplitude, quantal content, miniature EJP (mEJP) frequency or mEJP amplitude at the neuromuscular junction.

Overexpression of SytIVScer\UAS.cLa in neurons, driven by Scer\GAL4elav.PLu creates larvae that when tested electrophysiologically by stimulation of the neuromuscular junction, produce peak amplitudes of the excitatory junction potential that are significantly (about 20%) lower than controls. Continued stimulation showed no further depletion over time. No decrease in muscle miniature excitatory junction potential (MEJP) amplitude or muscle resting membrane potential is seen in SytIVScer\UAS.cLa,Scer\GAL4elav.PLu larvae, however MEJP frequency is decreased by about a third.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Overexpression of SytIVScer\UAS.cLa, under the regulation of both Scer\GAL4elav-C155 and Scer\GAL4elav.PLu does not rescue the sytAD4/sytN13 phenotype. However, this overexpression does result in a 96% reduction in the expected Mendelian ratios. Overexpression of SytIVScer\UAS.cLa, under the regulation of Scer\GAL4elav-C155, in a sytAD4/sytN13 null mutant background has no effect on the synaptic transmission defects. Only slow release reflecting the asynchronous component of fusion is observed with the evoked EJP unchanged from the null mutant alone.

EJP amplitude at the neuromuscular junction is restored to near wild-type levels in sytAD4 homozygous larvae if they are also expressing SytIVScer\UAS.cLa under the control of Scer\GAL4elav.PLu. The increase in frequency of mEJPs is also rescued to wild-type in these larvae.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

The lack of high-frequency-stimulation-induced miniature release (HFMR) seen in Df(3R)rn16 animals is rescued by expression of SytIVScer\UAS.cLa under the control of Scer\GAL4Mhc.PW (postsynaptic expression) but not by expression of SytIVScer\UAS.cLa under the control of Scer\GAL4elav-C155 (presynaptic expression).

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Syt4Scer\UAS.cLa
Syt4UAS.cLa
SytIVScer\UAS.cLa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Littleton
Secondary FlyBase IDs
    References (6)