Allele Dmel\SmD3^k09037

General Information
Symbol	Dmel\SmD3^k09037	Species	D. melanogaster
Name		FlyBase ID	FBal0100401
Feature type	allele	Created / Updated	2006-05-14/2006-05-14
Associated gene	Dmel\SmD3

Allele class
Mutagen	P-element activity
Nature of the Allele
Allele class
Mutagen	P-element activity (Torok et al., 1993, BDGP Project Members, 1994-1999, Roch et al., 1998, Schenkel et al., 1999, Schenkel et al., 2002)
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Assay mode
Caused by insertion	P{lacW}SmD3^k09037 (Schenkel et al., 1999, BDGP Project Members, 1994-1999, Torok et al., 1993, Schenkel et al., 2002, Roch et al., 1998)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| recessive (Roch et al., 1998) lethal \| larval stage (with SmD3^k15401) (Schenkel et al., 2002) lethal \| larval stage (with SmD3^k13107) (Schenkel et al., 2002) lethal \| larval stage (with SmD3^k11803) (Schenkel et al., 2002) lethal \| larval stage (with SmD3^k09540) (Schenkel et al., 2002) lethal \| larval stage (with SmD3^lex47) (Schenkel et al., 2002) lethal \| larval stage (with SmD3^EP2176), with Scer\GAL4[-]: (Schenkel et al., 2002) lethal \| larval stage (with SmD3^ΔLR51) (Schenkel et al., 2002) lethal \| larval stage \| recessive (Schenkel et al., 2002) lethal \| pupal stage (Torok et al., 1993)
Phenotype Manifest In
	lymph gland (Schenkel et al., 2002, Torok et al., 1993) imaginal disc (Schenkel et al., 2002, Roch et al., 1998) larval salivary gland (Schenkel et al., 2002) larval optic lobe (Schenkel et al., 2002) dorsal mesothoracic disc (Schenkel et al., 2002) dorsal metathoracic disc (Schenkel et al., 2002) ventral thoracic disc (Schenkel et al., 2002) larval brain (Torok et al., 1993)
Detailed Description
	Statement Reference Elongated brain hemispheres, slightly abnormal folding in discs, lymph gland retains lobular structure. (Torok et al., 1993) Lethality occurs during third instar larval or pupal stages. Mutants exhibit visible disc abnormalities: very small or no discs. Clones cannot be induced in wild type discs. (Roch et al., 1998) Homozygous 12-day old larvae show overgrowth of the brain, hematopoietic organs and imaginal discs and a reduction in size of the salivary glands. The size of the digestive tract is nearly normal. (Schenkel et al., 2002)
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	SmD3^k15401 (Schenkel et al., 2002) SmD3^k13107 (Schenkel et al., 2002) SmD3^k11803 (Schenkel et al., 2002) SmD3^k09540 (Schenkel et al., 2002) SmD3^lex47 (Schenkel et al., 2002) SmD3^EP2176 (Schenkel et al., 2002) SmD3^ΔLR51 (Schenkel et al., 2002)
Rescued by	SmD3^k09037 is rescued by SmD3^+t2.3 (Schenkel et al., 2002)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	This allele was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was discarded from the gene disruption project prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Spradling (spradling@mail1.ciwemb.edu). P-element excision demonstrates the insertion to be the cause of lethality. (Torok et al., 1993) The level of SmD3 transcript is strongly reduced in P{lacW}SmD3^k09037 homozygotes compared to wild type, whereas Oda expression remains essentially unaffected. The P{lacW}SmD3^k09037 insertion appears to affect only SmD3 function and not Oda function (despite being within an Oda intron), since the lethality of P{lacW}SmD3^k09037 homozygotes is fully rescued by the P{SmD3.+t2.3} construct which contains the complete SmD3 gene. Complements: Oda^EP2104. (Schenkel et al., 2002)
Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	90/37 (Roch et al., 1998) guf^k09037 l(2)k090-37 (Schenkel et al., 2002, Schenkel et al., 1999) l(2)k09037^k09037 SmD3^k09037
Name Synonym
Secondary FlyBase IDs

References ( 5 )
Research paper	Schenkel et al., 2002, Genetics 161(2): 763--772 P elements inserted in the vicinity of or within the Drosophila snRNP SmD3 gene nested in the first intron of the Ornithine decarboxylase antizyme gene affect only the expression of SmD3. [FBrf0149027] Roch et al., 1998, Molec. gen. Genet. 257(2): 103--112 Screening of larval/pupal P-element induced lethals on the second chromosome in Drosophila melanogaster: clonal analysis and morphology of imaginal discs. [FBrf0100624] Torok et al., 1993, Genetics 135(1): 71--80 P-lacW insertional mutagenesis on the second chromosome of Drosophila melanogaster: isolation of lethals with different overgrowth phenotypes. [FBrf0058618]
Abstract	Schenkel et al., 1999, Europ. Dros. Res. Conf. 16: 214 Down-regulation of the expression of the spliceosome core protein SmD3 gene results in tissue overgrowth. [FBrf0110736]
Computer file	BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Allele Dmel\SmD3k09037

Allele Dmel\SmD3^k09037