Allele Dmel\Pi3K92E^A

General Information
Symbol	Dmel\Pi3K92E^A	Species	D. melanogaster
Name		FlyBase ID	FBal0100417
Feature type	allele	Associated gene	Dmel\Pi3K92E
Also Known As	Dp110^A, p110^A, Dp110A

Allele class
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	P-element activity (Weinkove et al., 1999)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	P{lacW}H^D179 (Weinkove et al., 1999)
Nature of the lesion	Statement Reference Deletion removes 9.3kb from nucleotide 26 in the H 5' UTR, though the H transcription unit and up to nucleotide 2296 in the Pi3K92E cDNA (coordinates of FBrf0091111). The predicted remnant of Pi3K92E would be amino acids 1-668 plus RCAV, followed by a stop, though this remnant is either unstable or not expressed. (Weinkove et al., 1999)
Caused by aberration	Df(3R)Pi3K92E^A (Williams et al., 2006, Weinkove et al., 1999)
Cytology
Phenotypic Data
Phenotypic Class
	decreased cell size \| recessive \| somatic clone (Bateman and McNeill, 2004) lethal (Orme et al., 2006) lethal \| recessive (Weinkove et al., 1999) planar polarity defective \| recessive \| somatic clone (Bateman and McNeill, 2004) small body (with Pi3K92E^2H1) (Halfar et al., 2001) viable (with Pi3K92E^2H1) (Halfar et al., 2001)
Phenotype Manifest In
	fat body (Weinkove et al., 1999, Scott et al., 2004) imaginal disc (Weinkove et al., 1999) larva (Weinkove et al., 1999) ommatidium \| somatic clone (Bateman and McNeill, 2004)
Detailed Description
	Statement Reference Flies with the genotype "Df(3R)Pi3K92E[A], P{HBS}/Df(3R)Pi3K92E[A]", which are effectively heterozygous for H but null for Pi3K92E, do not eclose. (Orme et al., 2006) Pi3K92E[A] exhibits a high-diapause phenotype, where the proportion of individuals in diapause is higher than in wild-type flies. (Williams et al., 2006) Pi3K92E^A mutants show no defects in opsin regulation. (Mikeladze-Dvali et al., 2005) Homozygous clones in the eye have very small ommatidia with rotation defects. (Bateman and McNeill, 2004) Autophagy induced in fat body even under non-starvation conditions. (Scott et al., 2004) The Pi3K92E^A/Pi3K92E^2H1 combination results in proportionally smaller than normal flies. (Halfar et al., 2001) Df(3R)Pi3K92E^A/Df(3R)Pi3K92E-B, P{HBS} mutants (null for Pi3K92E) stop growing early in the third larval instar. Though they continue to move and feed for approximately 20 days they never get any bigger. Larvae are transparent, lack imaginal discs and have reduced fat body. Nervous system, trachea and gut appear normal. Mutant clones in the eye reveal that Pi3K92E is autonomously required for adult cells to reach their normal size. (Weinkove et al., 1999)
External Data
Linkouts
Interactions
Phenotypic Class
NOT Suppressor of
Statement Reference Pi3K92E^2H1/Pi3K92E^A is a non-suppressor of visible phenotype of Ras85D^{G12V.Y40C.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Halfar et al., 2001)
Phenotype Manifest In
NOT Suppressor of
Statement Reference Pi3K92E^2H1/Pi3K92E^A is a non-suppressor of ommatidium phenotype of Ras85D^{G12V.Y40C.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Halfar et al., 2001)
Additional Comments
Genetic Interactions
Statement Reference The rough eye phenotype caused by expression of Ras85D^{G12V.Y40C.Scer\UAS} under the control of Scer\GAL4^GMR.PF is not suppressed by Pi3K92E^A/Pi3K92E^2H1. (Halfar et al., 2001)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	Pi3K92E^A is rescued by Pi3K92E^+t14.7 (Weinkove et al., 1999, Williams et al., 2006) Pi3K92E^A is rescued by Pi3K92E^T:Hsap\MYC (Orme et al., 2006) Pi3K92E^A is rescued by Pi3K92E^{t.RBD.T:Hsap\MYC} (Orme et al., 2006)
Partially rescued by	Pi3K92E^A is partially rescued by Pi3K92E^{t.RBD.T:Hsap\MYC} (Orme et al., 2006)
Comments	Expression of Pi3K92E[+t14.7] rescues the Pi3K92E[A] high-diapause phenotype. (Williams et al., 2006)
Stocks ( 3 )
Bloomington	25900 w^*; Df(3R)Pi3K92E^A, H^A Pi3K92E^A, P{HBS}3/TM6B, Tb¹ 25917 w¹¹¹⁸; P{H^+tLa}2; Df(3R)Pi3K92E^A, P{Myc-Dp110}1, Pi3K92E^A H^A 25906 w¹¹¹⁸; P{H^+tLa}2; Df(3R)Pi3K92E^A, H^A Pi3K92E^A, P{Myc-Dp110^RBD}1
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	Dp110^A (Coelho et al., 2005, Bateman and McNeill, 2004, Radimerski et al., 2002, Clancy et al., 2001, Halfar et al., 2001, Weinkove et al., 1999, Orme et al., 2006, Williams et al., 2006) Dp110A (Gaengel and Mlodzik, 2003, Mikeladze-Dvali et al., 2005) p110^A (Scott et al., 2004, Scott et al., 2004) Pi3K92E^A
Name Synonym
Secondary FlyBase IDs

References ( 12 )
Research paper	Orme et al., 2006, Nat. Cell Biol. 8(11): 1298--1302 Input from Ras is required for maximal PI(3)K signalling in Drosophila. [FBrf0193707] Williams et al., 2006, Proc. Natl. Acad. Sci. U.S.A. 103(43): 15911--15915 Natural variation in Drosophila melanogaster diapause due to the insulin-regulated PI3-kinase. [FBrf0192255] Coelho et al., 2005, Genetics 171(2): 597--614 A genetic screen for dominant modifiers of a small-wing phenotype in Drosophila melanogaster identifies proteins involved in splicing and translation. [FBrf0190712] Mikeladze-Dvali et al., 2005, Cell 122(5): 775--787 The growth regulators warts/lats and melted interact in a bistable loop to specify opposite fates in Drosophila R8 photoreceptors. [FBrf0187233] Bateman and McNeill, 2004, Cell 119(1): 87--96 Temporal control of differentiation by the insulin receptor/tor pathway in Drosophila. [FBrf0180028] Scott et al., 2004, Dev. Cell 7(2): 167--178 Role and regulation of starvation-induced autophagy in the Drosophila fat body. [FBrf0180117] Gaengel and Mlodzik, 2003, Development 130(22): 5413--5423 Egfr signaling regulates ommatidial rotation and cell motility in the Drosophila eye via MAPK/Pnt signaling and the Ras effector Canoe/AF6. [FBrf0167499] Radimerski et al., 2002, Nat. Cell Biol. 4(3): 251--255 dS6K-regulated cell growth is dPKB/dPI(3)K-independent, but requires dPDK1. [FBrf0145164] Clancy et al., 2001, Science 292(5514): 104--106 Extension of life-span by loss of CHICO, a Drosophila insulin receptor substrate protein. [FBrf0135946] Halfar et al., 2001, Development 128(9): 1687--1696 Ras controls growth, survival and differentiation in the Drosophila eye by different thresholds of MAP kinase activity. [FBrf0135730] Weinkove et al., 1999, Curr. Biol. 9(18): 1019--1029 Regulation of imaginal disc cell size, cell number and organ size by Drosophila class I(A) phosphoinositide 3-kinase and its adaptor. [FBrf0111517]
Supplementary material	Scott et al., 2004, Dev. Cell 7(2): Supplementary material. [FBrf0183440]

Allele Dmel\Pi3K92EA

Allele Dmel\Pi3K92E^A