FB2025_01 , released February 20, 2025
Allele: Dmel\Pi3K21BUAS.Tag:HA
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General Information
Symbol
Dmel\Pi3K21BUAS.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0100420
Feature type
allele
Associated gene
Dmel\Pi3K21B
Associated Insertion(s)
Carried in Construct
P{UAS-Pi3K21B.HA}
Also Known As
UAS-p60, UAS-Dp60
Key Links
Transgenic product class
cDNA
(Weinkove et al., 1999)
wild_type
(Weinkove et al., 1999)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Weinkove et al., 1999)
wild_type
(Weinkove et al., 1999)
Mutagen
in vitro construct
(Weinkove et al., 1999)
Progenitor genotype
Carried in construct
P{UAS-Pi3K21B.HA}
Cytology
Description

Expression of a full length Pi3K21B cDNA tagged with Tag:HA is governed by UASt.

(Weinkove et al., 1999)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Pi3K21B
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  Alzheimer's disease 1
      modeled by Hsap\APPAβ42.UAS.cUa
      (Cheng et al., 2021)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Pi3K21BScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4elav.PU (in combination with a Gal80[ts] transgene to restrict expression to the adult stage) results in increased sensitivity to ethanol-induced sedation, as compared to controls.

      (Acevedo et al., 2015)

      Over-expression of Pi3K21BScer\UAS.T:Ivir\HA1 driven by Scer\GAL4da.PU results in the majority of embryos failing to hatch; larvae that do hatch live for 5-7 days as first instar larvae without growing in size, and have morphological abnormalities in the fat body (larger lipid droplets forming in the adipocytes).

      Over-expression of Pi3K21BScer\UAS.T:Ivir\HA1 driven by Scer\GAL4en.PU results in pupal lethality and significantly reduced pupa size, compared to controls; when expression is driven by Scer\GAL4Bx-MS1096 in the wing disc, adult wings are small and have curly edges.

      (Ejeskär et al., 2015)

      Expression of Pi3K21BScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Cg.PA leads to lethality in larval and pupal stages, with severe reductions in larval fat body mass and fat body cell size, as compared to controls.

      (Schmitt et al., 2015)

      Central nervous system neuroblasts of fed larvae expressing Pi3K21BScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Cg.PA show no significant difference in EdU incorporation compared to controls.

      Central nervous system neuroblasts of fed larvae expressing Pi3K21BScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4nab show a reduction in EdU incorporation compared to controls.

      Expression of Pi3K21BScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Ilp2.PR results in a decrease in the mass of third instar larvae compared to controls.

      Central nervous system neuroblasts of fed second instar larvae expressing Pi3K21BScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4repo.PU show a decrease in EdU incorporation compared to controls.

      (Sousa-Nunes et al., 2011)

      Expression of Pi3K21BScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4wor.PA results in premature death of mushroom body neuroblasts compared to controls.

      (Siegrist et al., 2010)

      The salivary glands of Pi3K21BScer\UAS.T:Ivir\HA1; Scer\GAL4ptc-559.1 larvae are small and their cells do not have endoreplicated DNA.

      (Pierce et al., 2004)

      When expression is driven by Scer\GAL4Lsp2.PH, relatively late in the larval fat body, no effect on autophagic activity of fat droplet aggregation is detectable.

      (Rusten et al., 2004)

      Larvae expressing Pi3K21BScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Act5C.PP or Scer\GAL4Adh.PF remain growth arrested in the first instar for as long as 2 weeks. Clones of cells expressing Pi3K21BScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Act5C.PP in the larval salivary glands and fat body are greatly reduced in size and have much smaller nuclei with far less DNA than adjacent control cells. Expression of Pi3K21BScer\UAS.T:Ivir\HA1 in third larval instar fat body cells under the control of Scer\GAL4Act5C.PP causes the fat body cells to become more translucent. Expression of Pi3K21BScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Adh.PF, Scer\GAL4en-e16E or Scer\GAL4Act5C.PP has no effect on viability under starvation conditions.

      (Britton et al., 2002)

      When expression is driven is imaginal disc cells by Scer\GAL4Act5C.PP the size of the cells is reduced (as measured by FSC (forward scatter)). Overexpression of Pi3K21BScer\UAS.T:Ivir\HA1 or Pi3K21BScer\UAS.Δ.T:Ivir\HA1 is sufficient to reduce the rate of increase of cell number.

      (Weinkove et al., 1999)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Statement
      Reference

      Pi3K21BUAS.Tag:HA, Scer\GAL4da.PU has partially lethal - majority die phenotype, suppressible | partially by Pi3K92EN.UAS, Scer\GAL4da.PU

      (Ejeskär et al., 2015)

      Pi3K21BUAS.Tag:HA, Scer\GAL4da.PU has partially lethal - majority die phenotype, suppressible by Pi3K92EUAS.Tag:MYC, Scer\GAL4da.PU

      (Ejeskär et al., 2015)

      Pi3K21BUAS.Tag:HA, Scer\GAL4da.PU has decreased body size | larval stage phenotype, suppressible by Pi3K92EN.UAS, Scer\GAL4da.PU

      (Ejeskär et al., 2015)

      Pi3K21BUAS.Tag:HA, Scer\GAL4da.PU has decreased body size | larval stage phenotype, suppressible by Pi3K92EUAS.Tag:MYC, Scer\GAL4da.PU

      (Ejeskär et al., 2015)

      Pi3K21BUAS.Tag:HA, Scer\GAL4da.PU has partially lethal - majority die phenotype, suppressible by Hsap\PIK3CDp37δ.UAS, Scer\GAL4da.PU

      (Ejeskär et al., 2015)

      Pi3K21BUAS.Tag:HA, Scer\GAL4da.PU has partially lethal - majority die phenotype, suppressible by Pi3K92E::Hsap\PIK3CDDp37.UAS, Scer\GAL4da.PU

      (Ejeskär et al., 2015)

      Pi3K21BUAS.Tag:HA, Scer\GAL4da.PU has decreased body size | larval stage phenotype, suppressible by Hsap\PIK3CDp37δ.UAS, Scer\GAL4da.PU

      (Ejeskär et al., 2015)

      Pi3K21BUAS.Tag:HA, Scer\GAL4da.PU has decreased body size | larval stage phenotype, suppressible by Pi3K92E::Hsap\PIK3CDDp37.UAS, Scer\GAL4da.PU

      (Ejeskär et al., 2015)

      Pi3K21BUAS.Tag:HA, Scer\GAL4en.PU has decreased body size | P-stage phenotype, suppressible | partially by Hsap\PIK3CDp37δ.UAS, Scer\GAL4en.PU

      (Ejeskär et al., 2015)

      Pi3K21BUAS.Tag:HA, Scer\GAL4ptc-559.1 has abnormal size | larval stage phenotype, suppressible by MycUAS.cZa, Scer\GAL4ptc-559.1

      (Pierce et al., 2004)
      NOT suppressed by
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of Pi3K92EN.Scer\UAS partially suppresses embryonic lethality seen with expression of Pi3K21BScer\UAS.T:Ivir\HA1 driven by Scer\GAL4da.PU. Co-expression of Pi3K92EScer\UAS.T:Hsap\MYC suppresses embryonic lethality seen with expression of Pi3K21BScer\UAS.T:Ivir\HA1 driven by Scer\GAL4da.PU.

      Co-expression of Pi3K21BScer\UAS.T:Ivir\HA1 or Pi3K92EN.Scer\UAS suppresses the small body phenotype in larvae with expression of Pi3K21BScer\UAS.T:Ivir\HA1 driven by Scer\GAL4da.PU, but larvae remain first instars for 2 weeks until they die and retain fat body abnormalities.

      (Ejeskär et al., 2015)

      The reduced size and block to endoreplication in the salivary glands of Pi3K21BScer\UAS.T:Ivir\HA1; Scer\GAL4ptc-559.1 larvae is partially suppressible by dmScer\UAS.cZa.

      (Pierce et al., 2004)
      Xenogenetic Interactions
      Statement
      Reference

      Co-expression of Hsap\PIK3CDp37δ.Scer\UAS or Pi3K92E::Hsap\PIK3CDDp37.Scer\UAS suppresses embryonic lethality seen with expression of Pi3K21BScer\UAS.T:Ivir\HA1 driven by Scer\GAL4da.PU.

      Co-expression of Hsap\PIK3CDp37δ.Scer\UAS or Pi3K92E::Hsap\PIK3CDDp37.Scer\UAS suppresses the small body phenotype in larvae with expression of Pi3K21BScer\UAS.T:Ivir\HA1 driven by Scer\GAL4da.PU, but larvae remain developmentally delayed and retain fat body abnormalities.

      Co-expression of Hsap\PIK3CDp37δ.Scer\UAS significantly partially suppresses the reduced pupa size seen with expression of Pi3K21BScer\UAS.T:Ivir\HA1 driven by Scer\GAL4en.PU. Co-expression of Hsap\PIK3CDp37δ.Scer\UAS does not suppress the reduced wing size seen with expression of Pi3K21BScer\UAS.T:Ivir\HA1 driven by Scer\GAL4Bx-MS1096.

      (Ejeskär et al., 2015)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Pi3K21BScer\UAS.T:Ivir\HA1
      Pi3K21BUAS.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (17)