Allele Dmel\Pi3K21B^{Scer\UAS.Δ.T:Ivir\HA1}

General Information
Symbol	Dmel\Pi3K21B^{Scer\UAS.Δ.T:Ivir\HA1}	Species	D. melanogaster
Name		FlyBase ID	FBal0100421
Feature type	allele	Associated gene	Dmel\Pi3K21B

Allele class
Mutagen	in vitro construct - deletion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - deletion (Weinkove et al., 1999)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	Pi3K21B^{Scer\UAS.T:Ivir\HA1} (Weinkove et al., 1999)
Nature of the lesion	Statement Reference Construct: Deletion of amino acids 466-558. (Weinkove et al., 1999)
Carried in construct	P{UAS-Pi3K21BΔ.HA} (Britton et al., 2002, Martin-Castellanos and Edgar, 2002, Gallio et al., 2004, Prober and Edgar, 2002, Weinkove et al., 1999, Scott et al., 2004, Scott et al., 2004, Yang and Baker, 2003, Shelly et al., 2009)
Tagged with	T:Ivir\HA1 (Weinkove et al., 1999, Britton et al., 2002, Martin-Castellanos and Edgar, 2002, Prober and Edgar, 2002, Yang and Baker, 2003, Gallio et al., 2004, Scott et al., 2004, Scott et al., 2004)
Cytology
Phenotypic Data
Phenotypic Class
	decreased cell size \| somatic clone, with Scer\GAL4^Act5C.PP (Britton et al., 2002, Martin-Castellanos and Edgar, 2002)
Phenotype Manifest In
	cell cycle \| somatic clone, with Scer\GAL4^Act5C.PP (Martin-Castellanos and Edgar, 2002) embryonic/larval fat body \| somatic clone, with Scer\GAL4^Act5C.PP (Britton et al., 2002) embryonic/larval ganglionic branch, with Scer\GAL4^bs-23.26 (Gallio et al., 2004) embryonic/larval salivary gland \| somatic clone, with Scer\GAL4^Act5C.PP (Britton et al., 2002) imaginal disc & cell, with Scer\GAL4^Act5C.PP (Weinkove et al., 1999)
Detailed Description
	Statement Reference Migration of about a fifth of ganglionic tracheal branches stalls in stage 16 Pi3K21B^{Scer\UAS.Δ.T:Ivir\HA1}; Scer\GAL4^bs-23.26 embryos. (Gallio et al., 2004) Caspase activation in cells posterior to the furrow in eye discs is only slightly reduced by Pi3K92E^{Scer\UAS.T:Hsap\MYC}; Scer\GAL4^GMR.PF. (Yang and Baker, 2003) Expression of Pi3K21B^{Scer\UAS.Δ.T:Ivir\HA1} under the control of Scer\GAL4^Act5C.PP or Scer\GAL4^Adh.PF inhibits larval growth. Clones of cells expressing Pi3K21B^{Scer\UAS.Δ.T:Ivir\HA1} under the control of Scer\GAL4^Act5C.PP in the salivary glands and fat body are greatly reduced in size and have much smaller nuclei with far less DNA than adjacent control cells. Expression of Pi3K21B^{Scer\UAS.Δ.T:Ivir\HA1} under the control of Scer\GAL4^Adh.PF, Scer\GAL4^en-e16E or Scer\GAL4^Act5C.PP has no effect on viability under starvation conditions. (Britton et al., 2002) Pi3K21B^{Scer\UAS.Δ.T:Ivir\HA1} clones in the wing disc (driven by Scer\GAL4^Act5C.PP) show a reduced cell size and delays in G1 progression. Clones have very few cells compared to controls. (Martin-Castellanos and Edgar, 2002) When expression is driven is imaginal disc cells by Scer\GAL4^Act5C.PP the size of the cells is reduced (as measured by FSC (forward scatter)). Cells are reduced in size in both G1 and S+G2. Overexpression of Pi3K21B^{Scer\UAS.T:Ivir\HA1} or Pi3K21B^{Scer\UAS.Δ.T:Ivir\HA1} is sufficient to reduce the rate of increase of cell number. (Weinkove et al., 1999)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference Pi3K21B^{Scer\UAS.Δ.T:Ivir\HA1}, Scer\GAL4^Act5C.PP is a suppressor of increased cell number \| somatic clone phenotype of Scer\GAL4^Act5C.PP, tkv^{Q253D.Scer\UAS.cNc} (Martin-Castellanos and Edgar, 2002)
Other
Statement Reference Pi3K21B^{Scer\UAS.Δ.T:Ivir\HA1}, Scer\GAL4^Act5C.PP, tkv^{Q253D.Scer\UAS.cNc} has increased cell number \| somatic clone phenotype (Martin-Castellanos and Edgar, 2002, Martin-Castellanos and Edgar, 2002)
Phenotype Manifest In
Suppressed by
Statement Reference Pi3K21B^{Scer\UAS.Δ.T:Ivir\HA1}, Scer\GAL4^Act5C.PP has cell cycle \| somatic clone phenotype, suppressible by tkv^{Q253D.Scer\UAS.cNc}, Scer\GAL4^Act5C.PP (Martin-Castellanos and Edgar, 2002)
Suppressor of
Statement Reference Pi3K21B^{Scer\UAS.Δ.T:Ivir\HA1}, Scer\GAL4^Act5C.PP is a suppressor of cell cycle \| somatic clone phenotype of Scer\GAL4^Act5C.PP, tkv^{Q253D.Scer\UAS.cNc} (Martin-Castellanos and Edgar, 2002)
Additional Comments
Genetic Interactions
Statement Reference The addition of tkv^{Q253D.Scer\UAS.cNc} to Pi3K21B^{Scer\UAS.Δ.T:Ivir\HA1} (driven by Scer\GAL4^Act5C.PP) suppresses the G1 delay phenotype seen in Pi3K21B^{Scer\UAS.Δ.T:Ivir\HA1}, Scer\GAL4^Act5C.PP clones alone. (Martin-Castellanos and Edgar, 2002)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Pi3K21B^{Scer\UAS.Δ.T:Ivir\HA1}
Name Synonym
Secondary FlyBase IDs

References ( 9 )
Research paper	Shelly et al., 2009, Immunity 30(4): 588--598 Autophagy is an essential component of Drosophila immunity against vesicular stomatitis virus. [FBrf0207677] Gallio et al., 2004, Development 131(15): 3605--3614 Rhomboid 3 orchestrates Slit-independent repulsion of tracheal branches at the CNS midline. [FBrf0179216] Scott et al., 2004, Dev. Cell 7(2): 167--178 Role and regulation of starvation-induced autophagy in the Drosophila fat body. [FBrf0180117] Yang and Baker, 2003, Dev. Cell 4(3): 359--369 Cell cycle withdrawal, progression, and cell survival regulation by EGFR and its effectors in the differentiating Drosophila eye. [FBrf0158858] Britton et al., 2002, Dev. Cell 2(2): 239--249 Drosophila's Insulin/PI3-Kinase pathway coordinates cellular metabolism with nutritional conditions. [FBrf0144797] Martin-Castellanos and Edgar, 2002, Development 129(4): 1003--1013 A characterization of the effects of Dpp signaling on cell growth and proliferation in the Drosophila wing. [FBrf0144813] Prober and Edgar, 2002, Genes Dev. 16(17): 2286--2299 Interactions between Ras1, dMyc, and dPI3K signaling in the developing Drosophila wing. [FBrf0151998] Weinkove et al., 1999, Curr. Biol. 9(18): 1019--1029 Regulation of imaginal disc cell size, cell number and organ size by Drosophila class I(A) phosphoinositide 3-kinase and its adaptor. [FBrf0111517]
Supplementary material	Scott et al., 2004, Dev. Cell 7(2): Supplementary material. [FBrf0183440]

Allele Dmel\Pi3K21BScer\UAS.Δ.T:Ivir\HA1

Allele Dmel\Pi3K21B^{Scer\UAS.Δ.T:Ivir\HA1}