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General Information
Symbol
Dmel\spenpoc361
Species
D. melanogaster
Name
FlyBase ID
FBal0101141
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference
    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In

    embryonic mesothoracic segment & sclerite | ectopic

    embryonic metathoracic segment & sclerite | ectopic

    sclerite & embryonic abdominal segment | ectopic

    Detailed Description
    Statement
    Reference

    Mutant embryos develop ectopic sclerites, which often look like the cuticular scar tissue that often surrounds the healed wound generated by a sterile needle in late-stage wild-type embryos.

    Mutant stage 17 embryos have failures in epidermal integrity; dye injected into the perivitelline space penetrates the body cavity in the mutant embryos.

    Generation of spenpoc361 mitotic clones in the wing imaginal disc, using the FLP/FRT technique, causes a wing vein phenotype in the adult. This includes loss of vein material, which is mostly in the distal part of the wing, ectopic formation of material around the veins, thickening of existing veins and slight mis-localization of both longitudinal and cross-veins. Wing hair polarity is also disrupted and bristles are misplaced at the wing margin. Generation of spenpoc361 mitotic clones in the anterior compartment of the wing disc, using the FLP/FRT technique, affects both the micro and macrochaetae on the notum of adults. Macrochaetae are lost in some parts of the notum, gained in others and mislocalized. Duplicated trichogens and thormogens are not observed, indicating that cell fate specification is not affected. In both spenpoc361 maternal and zygotic embryos, the neurons of the peripheral nervous system show an abnormal distribution and morphology, and can be either fewer or greater in number than in wild type.

    Mutant embryos derived from females containing homozygous spenpoc361 germline clones crossed to spen3/+ males (lacking both maternal and zygotic spen function) show alterations in the number of many peripheral and central nervous system cell types, and the development of other organs is affected. The number of lateral chordotonal organs in each abdominal hemisegment varies from 0 to 6, and is typically 4 (wild-type number is 5). Clusters containing the normal number are often disorganised. Midline development is defective. Commissures are missing or poorly separated in stage 15 embryos. All of the longitudinal axons are disrupted in stage 16 embryos and axons inappropriately cross the midline. The development of all motor axons pathways is defective; motor axons exit the central nervous system, pick the correct pathways, but fail to innervate their muscle targets. Muscle fibres are missing or disorganised.

    Hemizygotes show loss of anterior H piece and ectopic sclerites develop in the middle of T2 and T3. These ectopic sclerites do not form in the fields of the denticle belts. When the maternal contribution is reduced the phenotype is more marked, with the posterior mouth hooks, median tooth, hypostomal, ectostomal, and epistomal sclerites, dorsal bridge and lateralgraten all either missing or disrupted. The trunk region of the embryos appears to be largely unaffected. Denticle belts and filzkorper show slightly reduced pigmentation.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Enhanced by
    Statement
    Reference
    Enhancer of
    Statement
    Reference
    Suppressor of
    Phenotype Manifest In
    Enhanced by
    Suppressed by
    Statement
    Reference

    spenpoc361 has phenotype, suppressible by Antphs.PHTA

    spenpoc361 has embryonic mesothoracic segment & sclerite | ectopic phenotype, suppressible by tshUAS.cGa/Scer\GAL469B

    spenpoc361 has embryonic metathoracic segment & sclerite | ectopic phenotype, suppressible by tshUAS.cGa/Scer\GAL469B

    Enhancer of
    Statement
    Reference
    Suppressor of
    Statement
    Reference
    Other
    Additional Comments
    Genetic Interactions
    Statement
    Reference

    The additional presence of a ckunspecified mutation in mitotic spenpoc361 clones, generated in the wing disc, enhances the wing phenotype in adults. There is a greater excess of vein material in the double mutants, more misplacement of longitudinal and cross veins, and more misalignment and tufting of the thick trichomes at the wing margin. These clones are non-autonomous as they can affect vein morphology on the dorsal side of the wing when the clones are ventral and vice versa.

    Enhances the rough eye phenotype of E2fGMR.PD DpGMR.PD flies; the eyes are rougher and have more bristles. The second wave of S phases is abnormal in eye discs of these animals.

    Xenogenetic Interactions
    Statement
    Reference

    Flies expressing Hsap\CDKN1AGMR.PH have rough eyes. This phenotype is suppressed by spenpoc361. The second mitotic wave is absent in eye imaginal discs expressing Hsap\CDKN1AGMR.PH. This wave is partially restored in the presence of one copy of spenpoc361.

    Complementation and Rescue Data
    Images (0)
    Mutant
    Wild-type
    Stocks (0)
    Notes on Origin
    Discoverer
    Comments
    Comments

    spen alleles form an allelic series: from strongest to weakest spenpoc361 > spenpoc231 > spenE(CycE++)e9 > spenE(CycE++)D57.

    One of the six alleles mentioned but not named in FBrf0098246.

    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (4)
    Reported As
    Name Synonyms
    Secondary FlyBase IDs
      References (9)