Allele Dmel\robl^Z

General Information
Symbol	Dmel\robl^Z	Species	D. melanogaster
Name		FlyBase ID	FBal0101178
Feature type	allele	Associated gene	Dmel\robl

Allele class	neomorphic allele - genetic evidence
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	neomorphic allele - genetic evidence (Bowman et al., 1999)
Mutagen	ethyl methanesulfonate (Bowman et al., 1999)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference 193bp deletion in the middle of the transcription unit removing portions of intron 2 and exon 3. (Bowman et al., 1999)
Cytology
Phenotypic Data
Phenotypic Class
	decreased cell number \| somatic clone (Reuter et al., 2003) lethal \| larval stage \| recessive (Bowman et al., 1999) neuroanatomy defective \| somatic clone (Reuter et al., 2003) paralytic \| larval stage \| posterior \| recessive (Bowman et al., 1999)
Phenotype Manifest In
	adult mushroom body \| somatic clone (Reuter et al., 2003) dendrite \| somatic clone (Reuter et al., 2003) eye (Bowman et al., 1999) imaginal disc (Bowman et al., 1999) macrochaeta (Bowman et al., 1999) mitosis & nuclear chromosome (Bowman et al., 1999) mitotic anaphase (Bowman et al., 1999) mitotic cell cycle (Bowman et al., 1999) mitotic telophase (Bowman et al., 1999) segmental nerve (Bowman et al., 1999)
Detailed Description
	Statement Reference Mutant mushroom body neuroblast clones show a reduced cell number and a "spotty axon" phenotype. Single cell mushroom body neuron clones examined in the adult show a twofold reduction in total dendritic length and branching points compared to wild type. Axon swellings occur in these cells preferentially at the distal ends of axon branches. (Reuter et al., 2003) Homozygotes die during the late third larval instar stage. Homozygous larvae show a progressive posterior sluggish phenotype leading to complete posterior paralysis (the anterior of the larva remains noticeably mobile). There is a complete absence of imaginal tissue. Homozygous larval brains show a profound mitotic defect; there is a striking absence of prometaphase and metaphase mitotic figures. Only infrequent defective anaphase and telophase figures are seen. The few anaphase figures have severe bridging and lagging chromosomes. In addition, apparent telophase bridging in which DNA has become trapped between two dividing nuclei. Hemizygotes die as late pupae, and show posterior larval sluggishness, a peculiar tail flipping phenotype and accumulations of axonal cargo within their segmental nerves. The synaptic cargo tends to accumulate at the distal regions of axons with only infrequent proximal accumulations. The segmental nerves have swollen axons that have become filled with axonal cargo. These swollen axons are on average twice the diameter of the largest axon observed in wild type. Two classes of axonal accumulations are seen; small single component (small clear vesicles) accumulations and larger multi-component accumulations. Loss of axons and nerve degeneration is also seen. Hemizygotes that survive into late pupal stages have rough pupal eyes, missing bristles and the imaginal tissue is reduced in size. Larval brains have a significant number of mitotic defects; numerous polyploid mitotic figures are seen. Many of the polyploid figures show hypercondensation of the chromosomes. Abnormal anaphase figures are also seen with hypercondensed chromosomes and disorganisation of the chromosomes around the presumptive poles. Apparently normal mitotic figures are also seen. The mitotic index is fivefold higher than in wild type. (Bowman et al., 1999)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	robl^MB (Reuter et al., 2003)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	robl^Z
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Reuter et al., 2003, Development 130(6): 1203--1213 A mosaic genetic screen for genes necessary for Drosophila mushroom body neuronal morphogenesis. [FBrf0155698] Bowman et al., 1999, J. Cell Biol. 146(1): 165--179 Drosophila roadblock and Chlamydomonas LC7: a conserved family of dynein-associated proteins involved in axonal transport, flagellar motility, and mitosis. [FBrf0109386]

Allele Dmel\roblZ

Allele Dmel\robl^Z