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General Information
Symbol
Dmel\pkUAS.cGa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Gubb
FlyBase ID
FBal0101220
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-pk, UAS-Pkpk
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt sequences drive the Pk4a3 cDNA.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of pkScer\UAS.cGa under the control of Scer\GAL4ptc.PU results in wing hair polarity defects in the adult wings.

Expression of pkScer\UAS.cGa under the control of Scer\GAL4A51 or Scer\GAL4elav-C155 leads to bang sensitivity.

Expression of pkScer\UAS.cGa under the control of Scer\GAL4Toll-6-D42 results in normal polarity of motor neuron microtubules, but a significant increase in plus-end polymerization, as compared with controls.

Expression of pkScer\UAS.cGa under the control of Scer\GAL4ptc-559.1 results in wing hairs pointing towards the midline within the expression domain. Outside of the expression domain wing hair polarity is normal.

Scer\GAL4C-765-driven overexpression of pkScer\UAS.cGa results in variable localised changes in wing hair polarity and occasional cells producing extra hairs. Wing membrane ridge structure shows some deterioration compared to wild-types, but overall ridge orientation is unchanged.

Scer\GAL4Bx-MS1096-driven overexpression of pkScer\UAS.cGa results in disruption of wing hair polarity and ridge formation, but does not produce consistent wing hair or wing membrane ridge reorientation.

Expression of pkScer\UAS.cGa under the control of Scer\GAL4dpp.blk1 in a stripe along the proximo-distal axis of the wing results in a reorientation of wing hairs, which now point towards from the expression domain.

In pkScer\UAS.cGa; Scer\GAL4╬▒Tub84B.PL adults, planar polarity in the abdomen is reversed in parts of the anterior compartment of each segment, but is normal in the posterior compartments.

When expression is driven by Scer\GAL4ptc-559.1 hairs point towards the midline in the C region of the wing.

When pkScer\UAS.cGa is expressed as clones in the wing (driven by Scer\GAL4Ubx.PdC) produces a cell non-autonomous phenotype where cells distal to the clones point toward the clone. When pkScer\UAS.cGa is driven by Scer\GAL4ptc-559.1, the wing hairs in the region between wing vein L3 and L4, distal to the anterior crossvein point towards the midline of the wing, unlike wild-type wings, where the wing hairs point distally.

When pkScer\UAS.cGa is driven by Scer\GAL4en-e16E, hairs fail to migrate from the distal vertex of pupal wing cells of these mutants, unlike wild-type. Over expression of pkScer\UAS.cGa when driven by Scer\GAL4da.G32 or Scer\GAL4C-765 gives an extreme tarsal duplication phenotype, including a duplicated socket structure in the proximal T1 segment. Defects are also seen in T2, T3 and T4. Also, when driven by Scer\GAL4da.G32, swirls are seen in the wing hair orientation with different patterns from wing to wing.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhancer of
Statement
Reference
Phenotype Manifest In
NOT Enhancer of
Statement
Reference
Suppressor of
Statement
Reference

Scer\GAL4ptc-559.1/pkUAS.cGa is a suppressor of wing hair & 1st posterior cell phenotype of in1

Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescues
Comments

pkScer\UAS.cGa driven by Scer\GAL4C-765 rescues the wing phenotypes of pk30 flies. The triple row bristles are wild-type in appearance and the wing hairs are almost wild-type in appearance.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
pkScer\UAS.cGa
pkUAS.cGa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Gubb
Secondary FlyBase IDs
    References (15)