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General Information
Symbol
Dmel\pk30
Species
D. melanogaster
Name
FlyBase ID
FBal0101223
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
pkpk30
Allele class
Mutagen
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Sequence analysis reveals that Df(2R)pk30 has a deletion of 1306bp extending from bp 27 of the pk 5' exon to 7bp 5' of the second intron of Spn43Ab.

Lesion present between 0 and 4.8kb downstream of zero (zero being 973bp proximal to the pk transcription start site.). The 5' start of pk open reading frame is deleted.

Caused by aberration
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

pk30/+ flies do not exhibit significantly increased bang sensitivity as compared to wild type.

pk30 mutant wings display strong hair orientation defects, but very few if any multiple hairs.

Wing membrane ridges in the anterior region of the wings of wild-type flies have an anterior-posterior orientation, but have a proximal-distal orientation in a pk30 mutant.

pk30 mutant wing hairs display abnormal polarity across the wing, characterised by frequent whorls and 'stacking flaws'.

Causes no embryonic phenotype even when homozygous mutant embryos develop from homozygous mutant mothers. Causes an extreme polarity phenotype in the wing and notum. Radial and cruciform discontinuities in wing hair orientation can be seen between veins 2 and 3, and cruciform discontinuities are seen posterior to vein 5. Denticle belt morphology and denticle orientation remains wild-type. The individual cells in the pk30 wing are packed in a more irregular fashion than wild-type, exhibiting irregular cell shapes, including trapedoizal, cuboidal and square (as opposed to hexagonal and pentagonal cell shapes seen in wild-type). These cells are sometimes associated with duplicated wing hairs. Wings in pkpk-sple-13/pk30 flies show a phenotype stronger than pkpk-sple-13/pkpk-sple-13 and weaker than pk30/pk30.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

pk30 has visible | recessive phenotype, enhanceable by Rab23[+]/Rab2351

Suppressed by
Statement
Reference
Enhancer of
Statement
Reference

pk[+]/pk30 is an enhancer of neuroanatomy defective phenotype of DAAMEx1

Other
Statement
Reference
Phenotype Manifest In
Enhanced by
Statement
Reference

pk30 has wing hair phenotype, enhanceable by Rab23[+]/Rab2351

Suppressed by
Statement
Reference

pk30 has wing phenotype, suppressible by dshunspecified

pk30 has wing hair phenotype, suppressible by dshunspecified

Enhancer of
Statement
Reference

pk[+]/pk30 is an enhancer of mushroom body alpha-lobe phenotype of DAAMEx1

pk[+]/pk30 is an enhancer of mushroom body beta-lobe phenotype of DAAMEx1

Other
Additional Comments
Genetic Interactions
Statement
Reference

One copy of pk30 enhances the axonal projection defects seen in the αlobes of DAAMEx1 mutant mushroom bodies. The β lobe defects are also enhanced but not as strongly.

Wings of homozygous pk30, rhove-1 and vn1 triple mutant flies lack veins L2-5. Wing anterior hairs of these mutants consistently have a posterior component to their polarity and posterior hairs have an anterior component to their polarity.

Overexpression of aosScer\UAS.cMa under the control of Scer\GAL4Bx-MS1096 in homozygous pk30 mutants induces partial loss of longitudinal wing veins. The discontinuity in hair and ridge orientation maps to the site of the L3 vein.

In comparison to a pk30 homozygote, ft1, pk30 mutant wing hair polarity is more distal in both the anterior and in the distal posterior regions.

Expression of dsGD14350 under the control of Scer\GAL4Bx-MS1096 in the dorsal region of pk30-mutant wing modifies the pk30 hair phenotype to a more distal polarity in the anterior and distal posterior regions.

In lftTG2, pk30 double mutant wings, the pk30 hair phenotype is modified to a more distal polarity in the anterior and distal posterior regions.

Hair polarity on fjd1, pk30 double mutant wings is more distal than the pk30 phenotype.

When ftScer\UAS.cMa, dsScer\UAS.cTa or fjScer\UAS.cZa are overexpressed under the control of Scer\GAL4Bx-MS1096 in a pk30 mutant wing, the pk30 wing hair phenotype is modified to a more distal polarity in the anterior and distal posterior regions.

Expression of dsGD14350 under the control of Scer\GAL4salm-459.2 in a pk30 mutant wings results in changes in wing hair polarity (relative to the wild-type pattern) outside of the main Scer\GAL4salm-459.2 expression domain.

Expression of ftGD881 under the control of Scer\GAL4salm-459.2 in a pk30 mutant wings results in changes in wing hair polarity (relative to the wild-type pattern) outside of the main Scer\GAL4salm-459.2 expression domain.

Overexpression of dsScer\UAS.cTa under the control of Scer\GAL4salm-459.2 in a pk30 mutant wings results in changes in wing hair polarity (relative to the wild-type pattern) outside of the main Scer\GAL4salm-459.2 expression domain.

Overexpression of ftScer\UAS.cMa under the control of Scer\GAL4salm-459.2 in a pk30 mutant wings results in changes in wing hair polarity (relative to the wild-type pattern) outside of the main Scer\GAL4salm-459.2 expression domain.

Overexpression of fjScer\UAS.cZa under the control of Scer\GAL4salm-459.2 in a pk30 mutant wings results in changes in wing hair polarity (relative to the wild-type pattern) outside of the main Scer\GAL4salm-459.2 expression domain.

When flies of the genotype Scer\GAL4Bx-MS1096/+; pk30, dsGD14350/pk30, Scer\GAL80ts.αTub84B are cultivated continuously at 18[o]C, they show a typical pk30 mutant wing phenotype.

When flies of the genotype Scer\GAL4Bx-MS1096/+; pk30, dsGD14350/pk30, Scer\GAL80ts.αTub84B are cultivated continuously at 30[o]C, they display wing morphology typical of reduced ds activity combined with more distal wing hair polarity than a pk30 mutant.

When flies of the genotype Scer\GAL4Bx-MS1096/+; pk30, dsGD14350/pk30, Scer\GAL80ts.αTub84B are shifted from 18[o]C to 30[o]C during pupal development, they show close to wild-type wing morphology, but a wing hair phenotype that is dependent upon timing of the temperature shift. Flies shifted before 30 hours after puparium formation (a.p.f), display the more distal wing hair polarity typical of continuous ds knockdown and a significant modification of the pk30 wing hair phenotype is observed when pupae are shifted 36 hours a.p.f. However, pupae shifted after 40 hors a.p.f. display wing hair polarity phenotypes within the range of pk30 mutant wings.

Heterozygous Rab2351 significantly increases the number of multiple wing hairs in pk30 mutants.

The double mutant combination pk30, Rab2351 displays a very high number of multiple wing hairs and strong hair orientation defects. The orientation defects in pk30, Rab2351 double mutants seem weaker than in pk30 single mutants.

dshunspecified suppresses the extreme triple row phenotype of pk30 to one that is phenotypically dshunspecified. The wing blade phenotype is intermediate between the two single mutants.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
References (10)