FlyBase Allele Report: Dmel\pk[30]

General Information

Symbol

Dmel\pk³⁰

Species

D. melanogaster

Name

FlyBase ID

FBal0101223

Feature type

allele

Associated gene

Dmel\pk

Associated Insertion(s)

Carried in Construct

Also Known As

pk^pk30

Key Links

Allele class

Mutagen

X ray

Nature of the Allele

Allele class

Mutagen

X ray

(Gubb et al., 1999)

Progenitor genotype

Caused by aberration

Df(2R)pk³⁰

Cytology

Description

Sequence analysis reveals that Df(2R)pk³⁰ has a deletion of 1306bp extending from bp 27 of the pk 5' exon to 7bp 5' of the second intron of Spn43Ab.

(Green et al., 2000)

Lesion present between 0 and 4.8kb downstream of zero (zero being 973bp proximal to the pk transcription start site.). The 5' start of pk open reading frame is deleted.

(Gubb et al., 1999)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal planar polarity

(Rousset et al., 2001, Gubb et al., 1999)

abnormal planar polarity | recessive

(Pataki et al., 2010)

visible | recessive

(Pataki et al., 2010, Doyle et al., 2008, Gubb et al., 1999)

Phenotype Manifest In

scutum

(Gubb et al., 1999)

wing

(Rousset et al., 2001, Gubb et al., 1999)

wing & cell

(Gubb et al., 1999)

wing blade dorsal compartment | anterior

(Doyle et al., 2008)

wing hair

(Pataki et al., 2010, Doyle et al., 2008, Gubb et al., 1999)

Detailed Description

Statement

Reference

pk³⁰/+ flies do not exhibit significantly increased bang sensitivity as compared to wild type.

(Ehaideb et al., 2014)

pk³⁰ mutant wings display strong hair orientation defects, but very few if any multiple hairs.

(Pataki et al., 2010)

Wing membrane ridges in the anterior region of the wings of wild-type flies have an anterior-posterior orientation, but have a proximal-distal orientation in a pk³⁰ mutant.

pk³⁰ mutant wing hairs display abnormal polarity across the wing, characterised by frequent whorls and 'stacking flaws'.

(Doyle et al., 2008)

Causes no embryonic phenotype even when homozygous mutant embryos develop from homozygous mutant mothers. Causes an extreme polarity phenotype in the wing and notum. Radial and cruciform discontinuities in wing hair orientation can be seen between veins 2 and 3, and cruciform discontinuities are seen posterior to vein 5. Denticle belt morphology and denticle orientation remains wild-type. The individual cells in the pk³⁰ wing are packed in a more irregular fashion than wild-type, exhibiting irregular cell shapes, including trapedoizal, cuboidal and square (as opposed to hexagonal and pentagonal cell shapes seen in wild-type). These cells are sometimes associated with duplicated wing hairs. Wings in pk^pk-sple-13/pk³⁰ flies show a phenotype stronger than pk^pk-sple-13/pk^pk-sple-13 and weaker than pk³⁰/pk³⁰.

(Gubb et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

pk³⁰ has visible | recessive phenotype, enhanceable by Rab23[+]/Rab23⁵¹

(Pataki et al., 2010)

Suppressed by

Statement

Reference

pk³⁰ has abnormal planar polarity | recessive phenotype, suppressible by Rab23⁵¹

(Pataki et al., 2010)

Enhancer of

Statement

Reference

pk[+]/pk³⁰ is an enhancer of abnormal neuroanatomy phenotype of DAAM^Ex1

(Gombos et al., 2015)

Other

Statement

Reference

lft^TG2, pk³⁰ has visible phenotype

(Hogan et al., 2011)

lft^TG2, pk³⁰ has abnormal planar polarity phenotype

(Hogan et al., 2011)

fj^d1, pk³⁰ has visible phenotype

(Hogan et al., 2011)

fj^d1, pk³⁰ has abnormal planar polarity phenotype

(Hogan et al., 2011)

Scer\GAL4^Bx-MS1096, ft^UAS.cMa, pk³⁰ has visible phenotype

(Hogan et al., 2011)

Scer\GAL4^Bx-MS1096, ft^UAS.cMa, pk³⁰ has abnormal planar polarity phenotype

(Hogan et al., 2011)

Scer\GAL4^Bx-MS1096, ds^UAS.cTa, pk³⁰ has visible phenotype

(Hogan et al., 2011)

Scer\GAL4^Bx-MS1096, aos^UAS.cMa, pk³⁰ has visible phenotype

(Hogan et al., 2011)

Scer\GAL4^Bx-MS1096, ds^UAS.cTa, pk³⁰ has abnormal planar polarity phenotype

(Hogan et al., 2011)

Scer\GAL4^Bx-MS1096, fj^UAS.cZa, pk³⁰ has visible phenotype

(Hogan et al., 2011)

Scer\GAL4^Bx-MS1096, fj^UAS.cZa, pk³⁰ has abnormal planar polarity phenotype

(Hogan et al., 2011)

Scer\GAL4^salm-459.2, ds^GD14350, pk³⁰ has visible phenotype

(Hogan et al., 2011)

Scer\GAL4^salm-459.2, ds^GD14350, pk³⁰ has abnormal planar polarity phenotype

(Hogan et al., 2011)

Scer\GAL4^salm-459.2, ft^GD881, pk³⁰ has visible phenotype

(Hogan et al., 2011)

Scer\GAL4^salm-459.2, ft^GD881, pk³⁰ has abnormal planar polarity phenotype

(Hogan et al., 2011)

Scer\GAL4^salm-459.2, ds^UAS.cTa, pk³⁰ has visible phenotype

(Hogan et al., 2011)

Scer\GAL4^salm-459.2, ds^UAS.cTa, pk³⁰ has abnormal planar polarity phenotype

(Hogan et al., 2011)

Scer\GAL4^salm-459.2, ft^UAS.cMa, pk³⁰ has visible phenotype

(Hogan et al., 2011)

Scer\GAL4^salm-459.2, ft^UAS.cMa, pk³⁰ has abnormal planar polarity phenotype

(Hogan et al., 2011)

Scer\GAL4^salm-459.2, fj^UAS.cZa, pk³⁰ has visible phenotype

(Hogan et al., 2011)

Scer\GAL4^salm-459.2, fj^UAS.cZa, pk³⁰ has abnormal planar polarity phenotype

(Hogan et al., 2011)

ft¹, pk³⁰ has visible phenotype

(Hogan et al., 2011)

Scer\GAL4^Bx-MS1096, Scer\GAL80^ts.αTub84B, ds^GD14350, pk³⁰ has visible phenotype

(Hogan et al., 2011)

Scer\GAL4^Bx-MS1096, Scer\GAL80^ts.αTub84B, ds^GD14350, pk³⁰ has abnormal planar polarity phenotype

(Hogan et al., 2011)

pk³⁰, rho^ve-1, vn¹ has visible phenotype

(Hogan et al., 2011)

pk³⁰, rho^ve-1, vn¹ has abnormal planar polarity phenotype

(Hogan et al., 2011)

ft¹, pk³⁰ has abnormal planar polarity phenotype

(Hogan et al., 2011)

Scer\GAL4^Bx-MS1096, ds^GD14350, pk³⁰ has visible phenotype

(Hogan et al., 2011)

Scer\GAL4^Bx-MS1096, ds^GD14350, pk³⁰ has abnormal planar polarity phenotype

(Hogan et al., 2011)

Rab23⁵¹, pk³⁰ has visible | recessive phenotype

(Pataki et al., 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

pk³⁰ has wing hair phenotype, enhanceable by Rab23[+]/Rab23⁵¹

(Pataki et al., 2010)

Suppressed by

Statement

Reference

pk³⁰ has wing phenotype, suppressible by dsh^unspecified

(Gubb et al., 1999)

pk³⁰ has wing hair phenotype, suppressible by dsh^unspecified

(Gubb et al., 1999)

Enhancer of

Statement

Reference

pk[+]/pk³⁰ is an enhancer of adult mushroom body alpha-lobe phenotype of DAAM^Ex1

(Gombos et al., 2015)

pk[+]/pk³⁰ is an enhancer of adult mushroom body beta-lobe phenotype of DAAM^Ex1

(Gombos et al., 2015)

Other

Statement

Reference

lft^TG2, pk³⁰ has wing hair phenotype

(Hogan et al., 2011)

fj^d1, pk³⁰ has wing hair phenotype

(Hogan et al., 2011)

Scer\GAL4^Bx-MS1096, ft^UAS.cMa, pk³⁰ has wing hair phenotype

(Hogan et al., 2011)

Scer\GAL4^Bx-MS1096, ds^UAS.cTa, pk³⁰ has wing hair phenotype

(Hogan et al., 2011)

Scer\GAL4^Bx-MS1096, fj^UAS.cZa, pk³⁰ has wing hair phenotype

(Hogan et al., 2011)

Scer\GAL4^salm-459.2, ds^GD14350, pk³⁰ has wing hair phenotype

(Hogan et al., 2011)

Scer\GAL4^salm-459.2, ft^GD881, pk³⁰ has wing hair phenotype

(Hogan et al., 2011)

Scer\GAL4^Bx-MS1096, aos^UAS.cMa, pk³⁰ has wing vein phenotype

(Hogan et al., 2011)

Scer\GAL4^salm-459.2, ds^UAS.cTa, pk³⁰ has wing hair phenotype

(Hogan et al., 2011)

Scer\GAL4^salm-459.2, ft^UAS.cMa, pk³⁰ has wing hair phenotype

(Hogan et al., 2011)

Scer\GAL4^salm-459.2, fj^UAS.cZa, pk³⁰ has wing hair phenotype

(Hogan et al., 2011)

Scer\GAL4^Bx-MS1096, Scer\GAL80^ts.αTub84B, ds^GD14350, pk³⁰ has wing hair phenotype

(Hogan et al., 2011)

pk³⁰, rho^ve-1, vn¹ has wing hair phenotype

(Hogan et al., 2011)

pk³⁰, rho^ve-1, vn¹ has wing vein phenotype

(Hogan et al., 2011)

ft¹, pk³⁰ has wing hair phenotype

(Hogan et al., 2011)

Scer\GAL4^Bx-MS1096, ds^GD14350, pk³⁰ has wing hair phenotype

(Hogan et al., 2011)

Rab23⁵¹, pk³⁰ has wing hair phenotype

(Pataki et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of pk³⁰ enhances the axonal projection defects seen in the αlobes of DAAM^Ex1 mutant mushroom bodies. The β lobe defects are also enhanced but not as strongly.

(Gombos et al., 2015)

Wings of homozygous pk³⁰, rho^ve-1 and vn¹ triple mutant flies lack veins L2-5. Wing anterior hairs of these mutants consistently have a posterior component to their polarity and posterior hairs have an anterior component to their polarity.

Overexpression of aos^Scer\UAS.cMa under the control of Scer\GAL4^Bx-MS1096 in homozygous pk³⁰ mutants induces partial loss of longitudinal wing veins. The discontinuity in hair and ridge orientation maps to the site of the L3 vein.

In comparison to a pk³⁰ homozygote, ft¹, pk³⁰ mutant wing hair polarity is more distal in both the anterior and in the distal posterior regions.

Expression of ds^GD14350 under the control of Scer\GAL4^Bx-MS1096 in the dorsal region of pk³⁰-mutant wing modifies the pk³⁰ hair phenotype to a more distal polarity in the anterior and distal posterior regions.

In lft^TG2, pk³⁰ double mutant wings, the pk³⁰ hair phenotype is modified to a more distal polarity in the anterior and distal posterior regions.

Hair polarity on fj^d1, pk³⁰ double mutant wings is more distal than the pk³⁰ phenotype.

When ft^Scer\UAS.cMa, ds^Scer\UAS.cTa or fj^Scer\UAS.cZa are overexpressed under the control of Scer\GAL4^Bx-MS1096 in a pk³⁰ mutant wing, the pk³⁰ wing hair phenotype is modified to a more distal polarity in the anterior and distal posterior regions.

Expression of ds^GD14350 under the control of Scer\GAL4^salm-459.2 in a pk³⁰ mutant wings results in changes in wing hair polarity (relative to the wild-type pattern) outside of the main Scer\GAL4^salm-459.2 expression domain.

Expression of ft^GD881 under the control of Scer\GAL4^salm-459.2 in a pk³⁰ mutant wings results in changes in wing hair polarity (relative to the wild-type pattern) outside of the main Scer\GAL4^salm-459.2 expression domain.

Overexpression of ds^Scer\UAS.cTa under the control of Scer\GAL4^salm-459.2 in a pk³⁰ mutant wings results in changes in wing hair polarity (relative to the wild-type pattern) outside of the main Scer\GAL4^salm-459.2 expression domain.

Overexpression of ft^Scer\UAS.cMa under the control of Scer\GAL4^salm-459.2 in a pk³⁰ mutant wings results in changes in wing hair polarity (relative to the wild-type pattern) outside of the main Scer\GAL4^salm-459.2 expression domain.

Overexpression of fj^Scer\UAS.cZa under the control of Scer\GAL4^salm-459.2 in a pk³⁰ mutant wings results in changes in wing hair polarity (relative to the wild-type pattern) outside of the main Scer\GAL4^salm-459.2 expression domain.

When flies of the genotype Scer\GAL4^Bx-MS1096/+; pk³⁰, ds^GD14350/pk³⁰, Scer\GAL80^ts.αTub84B are cultivated continuously at 18[o]C, they show a typical pk³⁰ mutant wing phenotype.

When flies of the genotype Scer\GAL4^Bx-MS1096/+; pk³⁰, ds^GD14350/pk³⁰, Scer\GAL80^ts.αTub84B are cultivated continuously at 30[o]C, they display wing morphology typical of reduced ds activity combined with more distal wing hair polarity than a pk³⁰ mutant.

When flies of the genotype Scer\GAL4^Bx-MS1096/+; pk³⁰, ds^GD14350/pk³⁰, Scer\GAL80^ts.αTub84B are shifted from 18[o]C to 30[o]C during pupal development, they show close to wild-type wing morphology, but a wing hair phenotype that is dependent upon timing of the temperature shift. Flies shifted before 30 hours after puparium formation (a.p.f), display the more distal wing hair polarity typical of continuous ds knockdown and a significant modification of the pk³⁰ wing hair phenotype is observed when pupae are shifted 36 hours a.p.f. However, pupae shifted after 40 hors a.p.f. display wing hair polarity phenotypes within the range of pk³⁰ mutant wings.

(Hogan et al., 2011)

Heterozygous Rab23⁵¹ significantly increases the number of multiple wing hairs in pk³⁰ mutants.

The double mutant combination pk³⁰, Rab23⁵¹ displays a very high number of multiple wing hairs and strong hair orientation defects. The orientation defects in pk³⁰, Rab23⁵¹ double mutants seem weaker than in pk³⁰ single mutants.

(Pataki et al., 2010)

dsh^unspecified suppresses the extreme triple row phenotype of pk³⁰ to one that is phenotypically dsh^unspecified. The wing blade phenotype is intermediate between the two single mutants.

(Gubb et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

pk³⁰ is partially rescued by pk^UAS.cGa/Scer\GAL4^C-765

(Gubb et al., 1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

44229

Adc^b-1 pk³⁰ cn¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

pk³⁰

(Dye et al., 2021, Gombos et al., 2015, Merkel et al., 2014, Hogan et al., 2011)

pk^pk30

(Cho et al., 2022, Cho et al., 2020, Cho et al., 2015, Ehaideb et al., 2014, Hogan et al., 2011, Pataki et al., 2010, Doyle et al., 2008, Lin and Gubb, 2003)

Name Synonyms

Secondary FlyBase IDs

References (14)

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