FlyBase Allele Report: Dmel\pbl[UAS.cPa]

General Information

Symbol

Dmel\pbl^UAS.cPa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Prokopenko

FlyBase ID

FBal0101233

Feature type

allele

Associated gene

Dmel\pbl

Associated Insertion(s)

Carried in Construct

P{UAS-pbl.1A}

Also Known As

UAS-pbl, UAS-pebble

Key Links

Transgenic product class

cDNA

(Prokopenko et al., 1999)

wild_type

(Prokopenko et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Prokopenko et al., 1999)

wild_type

(Prokopenko et al., 1999)

Mutagen

in vitro construct

(Prokopenko et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-pbl.1A}

Cytology

Description

The 1A pbl cDNA is expressed under the control of UASt sequences.

(Prokopenko et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

pbl

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^GMR.PF

(Gregory et al., 2007, Prokopenko et al., 1999)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Gregory et al., 2007, Prokopenko et al., 1999)

interommatidial bristle, with Scer\GAL4^GMR.PF

(Prokopenko et al., 1999)

ommatidium, with Scer\GAL4^GMR.PF

(Prokopenko et al., 1999)

pigment cell, with Scer\GAL4^GMR.PF

(Prokopenko et al., 1999)

rhabdomere, with Scer\GAL4^GMR.PF

(Prokopenko et al., 1999)

Detailed Description

Statement

Reference

Expression of pbl^Scer\UAS.cPa under the control of Scer\GAL4^GMR.PF results in a 'rough eye' phenotype.

(Gregory et al., 2007)

Over-expression driven by Scer\GAL4^prd.RG1 does not result in a detectable phenotype. Over-expression of pbl^Scer\UAS.cPa in the eye driven by Scer\GAL4^GMR.PF results in a rough eye phenotype characterised by misshaped ommatidia, disrupted bristle distribution, misshapen rhabdomeres, and a significant increase in the number of pigment cells.

(Prokopenko et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, pbl^UAS.cPa has visible phenotype, enhanceable by stg^GS3120/Scer\GAL4^GMR.PF

(Gregory et al., 2007)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has visible phenotype, enhanceable by Cdk4^GS2301, Scer\GAL4^GMR.PF

(Gregory et al., 2007)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has visible phenotype, enhanceable by Lst^GS2301, Scer\GAL4^GMR.PF

(Gregory et al., 2007)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has visible phenotype, enhanceable by fray^GS3153/Scer\GAL4^GMR.PF

(Gregory et al., 2007)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has visible phenotype, enhanceable by Scer\GAL4^GMR.PF/tefu^GS13617

(Gregory et al., 2007)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has visible phenotype, enhanceable by CHES-1-like^GS1023, Scer\GAL4^GMR.PF

(Gregory et al., 2007)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has visible phenotype, enhanceable by wdb^GS3071/Scer\GAL4^GMR.PF

(Gregory et al., 2007)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has visible phenotype, enhanceable by grp^GS5044/Scer\GAL4^GMR.PF

(Gregory et al., 2007)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has visible phenotype, enhanceable by Diap1^GS9038, Scer\GAL4^GMR.PF

(Gregory et al., 2007)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has visible phenotype, enhanceable by Pdk1^GS10133, Scer\GAL4^GMR.PF

(Gregory et al., 2007)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has visible phenotype, enhanceable by CycH^GS9043, Scer\GAL4^GMR.PF

(Gregory et al., 2007)

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, pbl^UAS.cPa has visible phenotype, non-enhanceable by wun^GS10009/Scer\GAL4^GMR.PF

(Gregory et al., 2007)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has visible phenotype, non-enhanceable by Scer\GAL4^GMR.PF/aurA^GS7173

(Gregory et al., 2007)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has visible phenotype, non-enhanceable by Df(3L)Ar14-8

(Prokopenko et al., 1999)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has visible phenotype, non-enhanceable by Cdc42³

(Prokopenko et al., 1999)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has visible phenotype, non-enhanceable by Cdc42⁴

(Prokopenko et al., 1999)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, pbl^UAS.cPa has visible phenotype, suppressible | partially by Pitslre^GS10080, Scer\GAL4^GMR.PF

(Gregory et al., 2007)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has visible phenotype, suppressible | partially by Cdc14^GS9041, Scer\GAL4^GMR.PF

(Gregory et al., 2007)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has visible phenotype, suppressible | partially by sti^GS9053/Scer\GAL4^GMR.PF

(Gregory et al., 2007)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has visible phenotype, suppressible | partially by Mbs^GS13173, Scer\GAL4^GMR.PF

(Gregory et al., 2007)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has visible phenotype, suppressible | partially by Rac2^GS10622, Scer\GAL4^GMR.PF

(Gregory et al., 2007)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has visible phenotype, suppressible by Df(2R)Jp8

(Prokopenko et al., 1999)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has visible phenotype, suppressible by Rho1^72R

(Prokopenko et al., 1999)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, pbl^UAS.cPa has visible phenotype, non-suppressible by Scer\GAL4^GMR.PF/aurA^GS7173

(Gregory et al., 2007)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has visible phenotype, non-suppressible | partially by Rac2^GS10622, Scer\GAL4^GMR.PF

(Gregory et al., 2007)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has visible phenotype, non-suppressible by wun^GS10009/Scer\GAL4^GMR.PF

(Gregory et al., 2007)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has visible phenotype, non-suppressible by Df(3L)Ar14-8

(Prokopenko et al., 1999)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has visible phenotype, non-suppressible by Cdc42³

(Prokopenko et al., 1999)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has visible phenotype, non-suppressible by Cdc42⁴

(Prokopenko et al., 1999)

Enhancer of

Statement

Reference

pbl^UAS.cPa, Scer\GAL4^GMR.PU is an enhancer of abnormal eye color phenotype of Hsap\MECP2^R294X.UAS, Scer\GAL4^GMR.PU

(Cukier et al., 2008)

Other

Statement

Reference

RhoBTB^GS51, Scer\GAL4^GMR.PF, pbl^UAS.cPa has lethal phenotype

(Gregory et al., 2007)

Ide^GS51, Scer\GAL4^GMR.PF, pbl^UAS.cPa has lethal phenotype

(Gregory et al., 2007)

Ptpmeg^GS9137, Scer\GAL4^GMR.PF, pbl^UAS.cPa has lethal phenotype

(Gregory et al., 2007)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, pbl^UAS.cPa has eye phenotype, enhanceable by stg^GS3120/Scer\GAL4^GMR.PF

(Gregory et al., 2007)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has eye phenotype, enhanceable by Cdk4^GS2301, Scer\GAL4^GMR.PF

(Gregory et al., 2007)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has eye phenotype, enhanceable by Lst^GS2301, Scer\GAL4^GMR.PF

(Gregory et al., 2007)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has eye phenotype, enhanceable by fray^GS3153/Scer\GAL4^GMR.PF

(Gregory et al., 2007)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has eye phenotype, enhanceable by Scer\GAL4^GMR.PF/tefu^GS13617

(Gregory et al., 2007)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has eye phenotype, enhanceable by CHES-1-like^GS1023, Scer\GAL4^GMR.PF

(Gregory et al., 2007)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has eye phenotype, enhanceable by wdb^GS3071/Scer\GAL4^GMR.PF

(Gregory et al., 2007)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has eye phenotype, enhanceable by grp^GS5044/Scer\GAL4^GMR.PF

(Gregory et al., 2007)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has eye phenotype, enhanceable by Diap1^GS9038, Scer\GAL4^GMR.PF

(Gregory et al., 2007)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has eye phenotype, enhanceable by Pdk1^GS10133, Scer\GAL4^GMR.PF

(Gregory et al., 2007)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has eye phenotype, enhanceable by CycH^GS9043, Scer\GAL4^GMR.PF

(Gregory et al., 2007)

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, pbl^UAS.cPa has eye phenotype, non-enhanceable by wun^GS10009/Scer\GAL4^GMR.PF

(Gregory et al., 2007)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has eye phenotype, non-enhanceable by Scer\GAL4^GMR.PF/aurA^GS7173

(Gregory et al., 2007)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has eye phenotype, non-enhanceable by Rac2^GS10622, Scer\GAL4^GMR.PF

(Gregory et al., 2007)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has eye phenotype, non-enhanceable by Df(3L)Ar14-8

(Prokopenko et al., 1999)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has eye phenotype, non-enhanceable by Cdc42³

(Prokopenko et al., 1999)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has eye phenotype, non-enhanceable by Cdc42⁴

(Prokopenko et al., 1999)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, pbl^UAS.cPa has eye phenotype, suppressible | partially by Pitslre^GS10080, Scer\GAL4^GMR.PF

(Gregory et al., 2007)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has eye phenotype, suppressible | partially by Cdc14^GS9041, Scer\GAL4^GMR.PF

(Gregory et al., 2007)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has eye phenotype, suppressible | partially by sti^GS9053/Scer\GAL4^GMR.PF

(Gregory et al., 2007)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has eye phenotype, suppressible | partially by Mbs^GS13173, Scer\GAL4^GMR.PF

(Gregory et al., 2007)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has eye phenotype, suppressible by Df(2R)Jp8

(Prokopenko et al., 1999)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has ommatidium phenotype, suppressible by Df(2R)Jp8

(Prokopenko et al., 1999)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has interommatidial bristle phenotype, suppressible by Df(2R)Jp8

(Prokopenko et al., 1999)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has rhabdomere phenotype, suppressible by Df(2R)Jp8

(Prokopenko et al., 1999)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has pigment cell phenotype, suppressible by Df(2R)Jp8

(Prokopenko et al., 1999)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has eye phenotype, suppressible by Rho1^72R

(Prokopenko et al., 1999)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has ommatidium phenotype, suppressible by Rho1^72R

(Prokopenko et al., 1999)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has interommatidial bristle phenotype, suppressible by Rho1^72R

(Prokopenko et al., 1999)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has rhabdomere phenotype, suppressible by Rho1^72R

(Prokopenko et al., 1999)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has pigment cell phenotype, suppressible by Rho1^72R

(Prokopenko et al., 1999)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, pbl^UAS.cPa has eye phenotype, non-suppressible by wun^GS10009/Scer\GAL4^GMR.PF

(Gregory et al., 2007)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has eye phenotype, non-suppressible by Scer\GAL4^GMR.PF/aurA^GS7173

(Gregory et al., 2007)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has eye phenotype, non-suppressible by Rac2^GS10622, Scer\GAL4^GMR.PF

(Gregory et al., 2007)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has eye phenotype, non-suppressible by Df(3L)Ar14-8

(Prokopenko et al., 1999)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has eye phenotype, non-suppressible by Cdc42³

(Prokopenko et al., 1999)

Scer\GAL4^GMR.PF, pbl^UAS.cPa has eye phenotype, non-suppressible by Cdc42⁴

(Prokopenko et al., 1999)

Enhancer of

Statement

Reference

pbl^UAS.cPa, Scer\GAL4^GMR.PU is an enhancer of eye phenotype of Hsap\MECP2^UAS.FL, Scer\GAL4^GMR.PU

(Cukier et al., 2008)

pbl^UAS.cPa, Scer\GAL4^GMR.PU is an enhancer of eye phenotype of Hsap\MECP2^Δ166.UAS, Scer\GAL4^GMR.PU

(Cukier et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

The eye phenotype caused by expression of pbl^Scer\UAS.cPa by Scer\GAL4^GMR.PF is suppressed almost completely by Df(2R)Jp8, and Rho1^72R. The phenotype is dominantly suppressed by dia². Cdc42³, Cdc42⁴ and Df(3L)Ar14-8 have no effect on the phenotypes in the eye caused by pbl^Scer\UAS.cPa driven by Scer\GAL4^GMR.PF.

(Prokopenko et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^arm.PS/pbl^UAS.cPa rescues pbl^fortune

(Nakamura et al., 2013)

Scer\GAL4^byn-Gal4/pbl^UAS.cPa rescues pbl^fortune

(Nakamura et al., 2013)

Scer\GAL4^NP2432/pbl^UAS.cPa rescues pbl^fortune

(Nakamura et al., 2013)

Partially rescues

pbl^UAS.cPa partially rescues pbl^fortune

(Nakamura et al., 2013)

Scer\GAL4^twi.PB/pbl^UAS.cPa partially rescues pbl³

(Schumacher et al., 2004)

Comments

pbl^Scer\UAS.cPa reduces the frequency of defects in the left-right asymmetry of the hindgut by half in homozygous pbl^fortune embryos, even in the absence of a Scer\GAL4 driver. When expression is driven by Scer\GAL4^arm.PS, Scer\GAL4^byn-Gal4 or Scer\GAL4^NP2432 the left-right asymmetry are rescued. Expression driven by either Scer\GAL4^how-24B, Scer\GAL4^elav.PU or Scer\GAL4^48Y does not increase level of rescue above that seen without a Scer\GAL4 driver.

Expression of pbl^Scer\UAS.cPa under the control of Scer\GAL4^byn-Gal4 rescues the reduced number of cells in the epithelium of stage 13 pbl^fortune homozygous embryos.

(Nakamura et al., 2013)

Both cytokinesis and migration defects in the mesoderm of pbl³/Df(3L)pbl-NR embryos are rescued by pbl^Scer\UAS.cPa; Scer\GAL4^twi.PB. However, cytokinesis defects still result in multi-nucleated cells in the ectoderm of these embryos.

(Schumacher et al., 2004)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

pbl^Scer\UAS.cPa

pbl^UAS.cPa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Prokopenko

(Prokopenko et al., 1999)

Secondary FlyBase IDs

References (7)

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