The 1.94kb EcoR1-Sca1 and the 1.04kb Spe1(filled in)-EcoR1 fragments of the 1A pbl cDNA are expressed under the control of UASt sequences. Amino acids 497-549, at the C terminal of the DH domain, are removed.
When pblΔDH497-549.Scer\UAS is driven by Scer\GAL4Bx-MS1096 a multiple wing hair phenotype is seen. Nuclei in the wing are heterogeneous in size and, and the multiple wing hair phenotype is a consequence of a failure in cytokinesis.
Overexpression of pblΔDH497-549.Scer\UAS in the eye driven by Scer\GAL4GMR.PF, induces disruption of the ordered structure of the eye, with fusions of ommatidia, loss of many bristles, variation in numbers of rhabdomeres per ommatidium and decrease in the number of pigment cells. This phenotype is dominantly enhanced by mutations in pbl.