Allele Dmel\E(spl)m4-BFM^Scer\UAS.cAa

General Information
Symbol	Dmel\E(spl)m4-BFM^Scer\UAS.cAa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Apidianakis	FlyBase ID	FBal0101268
Feature type	allele	Associated gene	Dmel\E(spl)m4-BFM

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Apidianakis et al., 1999)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: The complete m4 cDNA is under the control of Scer\UAS sequences. (Apidianakis et al., 1999)
Carried in construct	P{UAS-m4.A} (Apidianakis et al., 1999, Tapanes-Castillo and Baylies, 2004, Kumar and Moses, 2001, Nagel et al., 2000, Kumar and Moses, 2001, San-Juán and Baonza, 2011, Parks and Muskavitch, 2009.2.4)
Cytology
Phenotypic Data
Phenotypic Class
	visible, with Scer\GAL4^455.2 (Apidianakis et al., 1999) visible, with Scer\GAL4^ap-md544 (Apidianakis et al., 1999) visible, with Scer\GAL4^pnr-MD237 (Apidianakis et al., 1999) visible, with Scer\GAL4^ptc-559.1 (Apidianakis et al., 1999) visible, with Scer\GAL4^sca-T3 (Apidianakis et al., 1999) visible \| somatic clone, with Scer\GAL4^Ubx.PdC (Apidianakis et al., 1999)
Phenotype Manifest In
	dorsal row & sensory mother cell, with Scer\GAL4^ap-md544 (Apidianakis et al., 1999) macrochaeta, with Scer\GAL4^455.2 (Apidianakis et al., 1999) macrochaeta, with Scer\GAL4^ap-md544 (Apidianakis et al., 1999) macrochaeta, with Scer\GAL4^pnr-MD237 (Apidianakis et al., 1999) macrochaeta, with Scer\GAL4^ptc-559.1 (Apidianakis et al., 1999) macrochaeta, with Scer\GAL4^sca-T3 (Apidianakis et al., 1999) microchaeta, with Scer\GAL4^455.2 (Apidianakis et al., 1999) microchaeta, with Scer\GAL4^ap-md544 (Apidianakis et al., 1999) microchaeta, with Scer\GAL4^pnr-MD237 (Apidianakis et al., 1999) microchaeta, with Scer\GAL4^ptc-559.1 (Apidianakis et al., 1999) microchaeta, with Scer\GAL4^sca-T3 (Apidianakis et al., 1999) microchaeta \| somatic clone, with Scer\GAL4^Ubx.PdC (Apidianakis et al., 1999) scutellar bristle, with Scer\GAL4^455.2 (Apidianakis et al., 1999) scutellar bristle, with Scer\GAL4^ptc-559.1 (Apidianakis et al., 1999) scutellar bristle \| ectopic, with Scer\GAL4^455.2 (Apidianakis et al., 1999) sensillum campaniformium, with Scer\GAL4^455.2 (Apidianakis et al., 1999) sensillum campaniformium, with Scer\GAL4^ap-md544 (Apidianakis et al., 1999) sensillum campaniformium, with Scer\GAL4^pnr-MD237 (Apidianakis et al., 1999) sensillum campaniformium, with Scer\GAL4^ptc-559.1 (Apidianakis et al., 1999) sensillum campaniformium, with Scer\GAL4^sca-T3 (Apidianakis et al., 1999) sensillum campaniformium of anterior crossvein, with Scer\GAL4^ptc-559.1 (Apidianakis et al., 1999) sensillum campaniformium of anterior crossvein \| precursor, with Scer\GAL4^ap-md544 (Apidianakis et al., 1999) sensillum campaniformium of dorsal radius, with Scer\GAL4^ap-md544 (Apidianakis et al., 1999) sensory mother cell \| ectopic, with Scer\GAL4^bi-omb-Gal4 (Nagel et al., 2000)
Detailed Description
	Statement Reference No extra neuroblasts are seen when E(spl)m4-BFM[Scer\UAS.cAa] is expressed under the control of Scer\GAL4[wor.PA] or Scer\GAL4[elav.PU]. (San-Juán and Baonza, 2011) Expression of m4^Scer\UAS.cAa under the control of Scer\GAL4^ey.PB has no effect on either eye or antenna disc development. (Kumar and Moses, 2001) Expression of m4^Scer\UAS.cAa when driven by Scer\GAL4^bi-omb-Gal4 in the wing disc leads to an increase in the numbers of sensory organ precursors seen. (Nagel et al., 2000) Flies with m4^Scer\UAS.cAa under the control of Scer\GAL4^ptc-559.1, Scer\GAL4^sca-T3, Scer\GAL4^455.2, Scer\GAL4^pnr-MD237 or Scer\GAL4^ap-md544, show duplications of sensillum campaniformium, macrochaetae and microchaetae in their normal locations that are frequently tufted together. No effects are seen in the wing. Flies with m4^Scer\UAS.cAa under the control of Scer\GAL4^ptc-559.1, show duplications of the scutellar bristles and the anterior cross-vein campaniform sensilla in their normal locations. If two copies of m4^Scer\UAS.cAa are present the number of bristles increases yet more. Flies with m4^Scer\UAS.cAa under the control of Scer\GAL4^455.2, show duplications of the scutellar bristles. Flies with m4^Scer\UAS.cAa under the control of Scer\GAL4^32B or Scer\GAL4^bi-md653 showed no wing pattern aberrations. m4^Scer\UAS.cAa, when driven by Scer\GAL4^ap-md544, creates clusters of wing margin sensory organ precursors (SOPs) in their normal locations in the dorsal row, where there would only be single SOPs in wild-type. Over commitment of the ACV and L3 precursors of campaniform sensilla are also seen. The N/numb mediated asymmetric divisions in the SOP lineage are not affected. In somatic clones where m4^Scer\UAS.cAa is over-expressed under the control of Scer\GAL4^Ubx.PdC, clustered microchaetae are seen, even at the clone boundary, suggesting the effect is acting at the level of signal reception. (Apidianakis et al., 1999)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Scer\GAL4^455.2, E(spl)m4-BFM^Scer\UAS.cAa has visible phenotype, enhanceable by Df(3R)gro^b32.2 (Apidianakis et al., 1999)
Suppressed by
Statement Reference Scer\GAL4^455.2, E(spl)m4-BFM^Scer\UAS.cAa has visible phenotype, suppressible by N^Ax-1 (Apidianakis et al., 1999)
NOT Suppressor of
Statement Reference E(spl)m4-BFM^Scer\UAS.cAa, Scer\GAL4^455.2 is a non-suppressor of visible phenotype of E(spl)m7-HLH^{Scer\UAS.cdCa}, Scer\GAL4^455.2 (Apidianakis et al., 1999) E(spl)m4-BFM^Scer\UAS.cAa, Scer\GAL4^ptc-559.1 is a non-suppressor of visible phenotype of E(spl)m7-HLH^{Scer\UAS.cdCa}, Scer\GAL4^ptc-559.1 (Apidianakis et al., 1999) E(spl)m4-BFM^Scer\UAS.cAa, Scer\GAL4^ptc-559.1 is a non-suppressor of visible phenotype of E(spl)m8-HLH^Scer\UAS.cLa, Scer\GAL4^ptc-559.1 (Apidianakis et al., 1999) E(spl)m4-BFM^Scer\UAS.cAa, Scer\GAL4^ptc-559.1 is a non-suppressor of visible phenotype of E(spl)mβ-HLH^{Scer\UAS.cdCa}, Scer\GAL4^ptc-559.1 (Apidianakis et al., 1999) E(spl)m4-BFM^Scer\UAS.cAa, Scer\GAL4^ptc-559.1 is a non-suppressor of visible phenotype of E(spl)mδ-HLH^{Scer\UAS.cdCa}, Scer\GAL4^ptc-559.1 (Apidianakis et al., 1999)
Other
Statement Reference N^264-40, Scer\GAL4^455.2, E(spl)m4-BFM^Scer\UAS.cAa has visible phenotype (Apidianakis et al., 1999)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^455.2, E(spl)m4-BFM^Scer\UAS.cAa has scutellar bristle phenotype, enhanceable by Df(3R)gro^b32.2 (Apidianakis et al., 1999) Scer\GAL4^ptc-559.1, E(spl)m4-BFM^Scer\UAS.cAa has scutellar bristle \| ectopic phenotype, enhanceable by Scer\GAL4^ptc-559.1/E(spl)mα-BFM^Scer\UAS.cAa (Apidianakis et al., 1999)
NOT Enhanced by
Statement Reference Scer\GAL4^ptc-559.1, E(spl)m4-BFM^Scer\UAS.cAa has scutellar bristle \| ectopic phenotype, non-enhanceable by bib² (Apidianakis et al., 1999) Scer\GAL4^ptc-559.1, E(spl)m4-BFM^Scer\UAS.cAa has scutellar bristle \| ectopic phenotype, non-enhanceable by Df(3R)Dl-BX6 (Apidianakis et al., 1999) Scer\GAL4^ptc-559.1, E(spl)m4-BFM^Scer\UAS.cAa has scutellar bristle \| ectopic phenotype, non-enhanceable by mamⁱ⁵¹¹ (Apidianakis et al., 1999) Scer\GAL4^ptc-559.1, E(spl)m4-BFM^Scer\UAS.cAa has scutellar bristle \| ectopic phenotype, non-enhanceable by neur^A101 (Apidianakis et al., 1999)
Suppressed by
Statement Reference Scer\GAL4^455.2, E(spl)m4-BFM^Scer\UAS.cAa has scutellar bristle phenotype, suppressible by N^Ax-1 (Apidianakis et al., 1999) Scer\GAL4^ptc-559.1, E(spl)m4-BFM^Scer\UAS.cAa has scutellar bristle phenotype, suppressible by H² (Apidianakis et al., 1999)
NOT suppressed by
Statement Reference Scer\GAL4^455.2, E(spl)m4-BFM^Scer\UAS.cAa has scutellar bristle \| ectopic phenotype, non-suppressible by E(spl)m7-HLH^{Scer\UAS.cdCa}, Scer\GAL4^455.2 (Apidianakis et al., 1999) Scer\GAL4^ptc-559.1, E(spl)m4-BFM^Scer\UAS.cAa has scutellar bristle \| ectopic phenotype, non-suppressible by bib² (Apidianakis et al., 1999) Scer\GAL4^ptc-559.1, E(spl)m4-BFM^Scer\UAS.cAa has scutellar bristle \| ectopic phenotype, non-suppressible by Df(3R)Dl-BX6 (Apidianakis et al., 1999) Scer\GAL4^ptc-559.1, E(spl)m4-BFM^Scer\UAS.cAa has scutellar bristle \| ectopic phenotype, non-suppressible by mamⁱ⁵¹¹ (Apidianakis et al., 1999) Scer\GAL4^ptc-559.1, E(spl)m4-BFM^Scer\UAS.cAa has scutellar bristle \| ectopic phenotype, non-suppressible by neur^A101 (Apidianakis et al., 1999)
NOT Suppressor of
Statement Reference E(spl)m4-BFM^Scer\UAS.cAa, Scer\GAL4^455.2 is a non-suppressor of scutellar bristle phenotype of E(spl)m7-HLH^{Scer\UAS.cdCa}, Scer\GAL4^455.2 (Apidianakis et al., 1999) E(spl)m4-BFM^Scer\UAS.cAa, Scer\GAL4^ptc-559.1 is a non-suppressor of scutellar bristle phenotype of E(spl)m7-HLH^{Scer\UAS.cdCa}, Scer\GAL4^ptc-559.1 (Apidianakis et al., 1999) E(spl)m4-BFM^Scer\UAS.cAa, Scer\GAL4^ptc-559.1 is a non-suppressor of scutellar bristle phenotype of E(spl)m8-HLH^Scer\UAS.cLa, Scer\GAL4^ptc-559.1 (Apidianakis et al., 1999) E(spl)m4-BFM^Scer\UAS.cAa, Scer\GAL4^ptc-559.1 is a non-suppressor of scutellar bristle phenotype of E(spl)mβ-HLH^{Scer\UAS.cdCa}, Scer\GAL4^ptc-559.1 (Apidianakis et al., 1999) E(spl)m4-BFM^Scer\UAS.cAa, Scer\GAL4^ptc-559.1 is a non-suppressor of scutellar bristle phenotype of E(spl)mδ-HLH^{Scer\UAS.cdCa}, Scer\GAL4^ptc-559.1 (Apidianakis et al., 1999)
Additional Comments
Genetic Interactions
Statement Reference mα^Scer\UAS.cAa enhances the number of extra scutellar bristles caused by expression of m4^Scer\UAS.cAa under the control of Scer\GAL4^ptc-559.1 m4^Scer\UAS.cAa driven by Scer\GAL4^ptc-559.1 cannot restore scutellar bristles to E(spl)^Scer\UAS.cLa, HLHm7^{Scer\UAS.cdCa}, HLHmδ^{Scer\UAS.cdCa} or HLHmβ^{Scer\UAS.cdCa} The scutellar bristle duplication phenotype caused by expression of m4^Scer\UAS.cAa under the control of Scer\GAL4^455.2 can be suppressed by a single copy of HLHm7^{Scer\UAS.cdCa}. m4^Scer\UAS.cAa, when driven by Scer\GAL4^ap-md544 in a Df(1)sc10-1 background, does not restore any chaetae lost in this deficiency. The presence of N^Ax-1 partially suppresses the supernumerary scutellar bristle phenotype seen in m4^Scer\UAS.cAa when driven by Scer\GAL4^455.2. The presence of H² partially suppresses the supernumerary scutellar bristle phenotype seen in m4^Scer\UAS.cAa when driven by Scer\GAL4^ptc-559.1. The presence of Df(3R)Dl-BX6, neur^A101, bib² or mamⁱ⁵¹¹ has no significant effect on the supernumerary scutellar bristle phenotype seen in m4^Scer\UAS.cAa when driven by Scer\GAL4^ptc-559.1. The presence of Df(3R)gro^b32.2 enhances the supernumerary scutellar bristle phenotype seen in m4^Scer\UAS.cAa when driven by Scer\GAL4^ptc-559.1. (Apidianakis et al., 1999)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 2 )
Bloomington	26679 w^; P{UAS-m4.A}15.5 26678 w^; P{UAS-m4.A}15.21
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	E(spl)m4-BFM^Scer\UAS.cAa m4^Scer\UAS.cAa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Apidianakis (Apidianakis et al., 1999)
Secondary FlyBase IDs

References ( 7 )
Research paper	San-Juán and Baonza, 2011, Dev. Biol. 352(1): 70--82 The bHLH factor deadpan is a direct target of Notch signaling and regulates neuroblast self-renewal in Drosophila. [FBrf0213150] Tapanes-Castillo and Baylies, 2004, Development 131(10): 2359--2372 Notch signaling patterns Drosophila mesodermal segments by regulating the bHLH transcription factor twist. [FBrf0174582] Kumar and Moses, 2001, Cell 104(5): 687--697 EGF receptor and Notch signaling act upstream of Eyeless/Pax6 to control eye specification. [FBrf0134490] Kumar and Moses, 2001, Development 128(14): 2689--2697 The EGF receptor and notch signaling pathways control the initiation of the morphogenetic furrow during Drosophila eye development. [FBrf0138358] Nagel et al., 2000, Mech. Dev. 98(1-2): 19--28 Neural hyperplasia induced by RNA interference with m4/m gene activity. [FBrf0133204] Apidianakis et al., 1999, Mech. Dev. 86(1,2): 39--50 Overexpression of the m4 and malpha genes of the E(spl)-complex antagonizes notch mediated lateral inhibition. [FBrf0111325]
Personal communication to FlyBase	Parks and Muskavitch, 2009.2.4, P{UAS-m4.A} insertions. P{UAS-m4.A} insertions. [FBrf0206651]

Allele Dmel\E(spl)m4-BFMScer\UAS.cAa

Allele Dmel\E(spl)m4-BFM^Scer\UAS.cAa