Allele Dmel\jar^a.Scer\UAS

General Information
Symbol	Dmel\jar^a.Scer\UAS	Species	D. melanogaster
Name	antisense construct	FlyBase ID	FBal0101360
Feature type	allele	Associated gene	Dmel\jar

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Deng et al., 1999)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of the jar cDNA in an antisense direction. (Deng et al., 1999)
Carried in construct	P{UAS-jar.a} (Geisbrecht and Montell, 2002, Deng et al., 1999)
Cytology
Phenotypic Data
Phenotypic Class
	lethal, with Scer\GAL4^c532 (Deng et al., 1999) lethal, with Scer\GAL4^C577 (Deng et al., 1999) lethal, with Scer\GAL4^C601a (Deng et al., 1999) lethal, with Scer\GAL4^C844 (Deng et al., 1999) lethal \| embryonic stage, with Scer\GAL4^C22c (Deng et al., 1999) lethal \| larval stage, with Scer\GAL4^bves-C648 (Deng et al., 1999) lethal \| larval stage, with Scer\GAL4^C612b (Deng et al., 1999) lethal \| larval stage, with Scer\GAL4^C673 (Deng et al., 1999) lethal \| larval stage, with Scer\GAL4^fng-c606 (Deng et al., 1999) lethal \| pharate adult stage, with Scer\GAL4^jar-C865 (Deng et al., 1999) lethal \| pupal stage, with Scer\GAL4^C714 (Deng et al., 1999) lethal \| pupal stage, with Scer\GAL4^C789 (Deng et al., 1999) small body, with Scer\GAL4^C726b (Deng et al., 1999) visible, with Scer\GAL4^C535 (Deng et al., 1999)
Phenotype Manifest In
	border follicle cell, with Scer\GAL4^slbo.2.6 (Geisbrecht and Montell, 2002) centripetally migrating follicle cell, with Scer\GAL4^c532 (Deng et al., 1999) dorsal denticle, with Scer\GAL4^C726b (Deng et al., 1999) egg chamber, with Scer\GAL4^c532 (Deng et al., 1999) filopodium & border follicle cell, with Scer\GAL4^slbo.2.6 (Geisbrecht and Montell, 2002) follicle cell, with Scer\GAL4^c532 (Deng et al., 1999) follicle cell & nucleus, with Scer\GAL4^c532 (Deng et al., 1999) leg, with Scer\GAL4^c532 (Deng et al., 1999) leg, with Scer\GAL4^C535 (Deng et al., 1999) nurse cell & nucleus, with Scer\GAL4^c532 (Deng et al., 1999) third instar larval cuticle, with Scer\GAL4^C726b (Deng et al., 1999) wing, with Scer\GAL4^c532 (Deng et al., 1999) wing, with Scer\GAL4^C535 (Deng et al., 1999)
Detailed Description
	Statement Reference Border cell migration is inhibited in jar^a.Scer\UAS; Scer\GAL4^slbo.2.6 ovaries. Border cells in stage 9 jar^a.Scer\UAS; Scer\GAL4^slbo.2.6 ovaries (n=50) lack protrusions commonly seen in wild-type border cells at this stage (35/152). (Geisbrecht and Montell, 2002) Flies expressing jar^a.Scer\UAS under the control of Scer\GAL4^fng-c606 die during the first larval instar stage. Flies expressing jar^a.Scer\UAS under the control of Scer\GAL4^C673 die during the second larval instar stage. The cuticle of third instar larvae expressing jar^a.Scer\UAS under the control of Scer\GAL4^C726b is abnormal; dark denticles are detected on both the ventral and dorsal sides of each segment (in wild-type larvae dark denticles are only detected on the ventral side, while the dorsal denticles are transparent). In some cases, the whole segment is surrounded by a circle of dark denticles. The organisation of the bristles both dorsally and ventrally often resembles the wild type ventral pattern. The larvae are much smaller than normal. Animals expressing jar^a.Scer\UAS under the control of Scer\GAL4^jar-C865 develop quite normally until late metamorphosis, but they fail to eclose from the pupal case. Many animals expressing jar^a.Scer\UAS under the control of Scer\GAL4^c532 die as pharate adults and fail to eclose from the pupal case. Those that hatch have unexpanded wings and malformed legs which are bent, shortened and thickened. The flies are less active than wild-type files and die 3-4 days after eclosion. Oogenesis is stopped at stage 9 in females (it is normal up to this stage) and egg chambers degenerate after this stage. In stage 9 egg chambers, the "columnar" follicle cells are no longer columnar. Completed migration of the follicle cells is never seen, with the columnar migration being arrested at the initiation stage in most degenerating egg chambers. In these egg chambers, follicle cells mostly have abnormal shapes and nurse cell nuclei are usually degenerated. Centripetal migration is never seen. The epithelium is often disrupted and there may be several cells in the layer. The nuclei are abnormally positioned in the follicle cells. Border cell migration appears to be normal in many stage 9 egg chambers. Expression of jar^a.Scer\UAS under the control of Scer\GAL4^C535 results in flies with malformed legs and unexpanded wings. (Deng et al., 1999)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Statement Reference Scer\GAL4^slbo.2.6, jar^a.Scer\UAS has border follicle cell phenotype, suppressible \| partially by shg^hs.PW (Geisbrecht and Montell, 2002) Scer\GAL4^slbo.2.6, jar^a.Scer\UAS has border follicle cell phenotype, suppressible \| partially by shg^Scer\UAS.cSa/Scer\GAL4^slbo.2.6 (Geisbrecht and Montell, 2002) Scer\GAL4^slbo.2.6, jar^a.Scer\UAS has border follicle cell phenotype, suppressible \| partially by slbo^Scer\UAS.cLa/Scer\GAL4^slbo.2.6 (Geisbrecht and Montell, 2002)
NOT suppressed by
Statement Reference Scer\GAL4^slbo.2.6, jar^a.Scer\UAS has border follicle cell phenotype, non-suppressible by Fak^{Scer\UAS.T:Ivir\HA1}, Scer\GAL4^slbo.2.6 (Geisbrecht and Montell, 2002)
Additional Comments
Genetic Interactions
Statement Reference The inhibition of border cell migration seen in the ovaries of jar^a.Scer\UAS; Scer\GAL4^slbo.2.6 flies is significantly suppressed by the presence of shg^hs.PW under heat shock condition (flies raised at 29^oC and given a 1 hour heat shock at 37^oC 6-10 hours prior to dissection.). Partial suppression is also seen in flies carrying slbo^Scer\UAS.cLa or shg^Scer\UAS.cSa, but not Fak56D^{Scer\UAS.T:Ivir\HA1}. (Geisbrecht and Montell, 2002)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescued by	jar^a.Scer\UAS is partially rescued by jar^Hsp83.PH/Scer\GAL4^slbo.2.6 (Geisbrecht and Montell, 2002)
Comments	jar^Hsp83.PH + heat shock, partially rescues border cell migration in the ovaries of jar^a.Scer\UAS; Scer\GAL4^slbo.2.6 flies. (Flies raised at 29^oC and given a 1 hour heat shock at 37^oC 6-10 hours prior to dissection.) (Geisbrecht and Montell, 2002)
Stocks ( 0 )

Notes on Origin
Discoverer	Bownes

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	jar^a.Scer\UAS
Name Synonym	antisense construct (Deng et al., 1999)
Secondary FlyBase IDs

References ( 2 )
Research paper	Geisbrecht and Montell, 2002, Nat. Cell Biol. 4(8): 616--620 Myosin VI is required for E-cadherin-mediated border cell migration. [FBrf0151440] Deng et al., 1999, J. Cell Sci. 112(21): 3677--3690 A targeted gene silencing technique shows that Drosophila myosin VI is required for egg chamber and imaginal disc morphogenesis. [FBrf0111848]

Allele Dmel\jara.Scer\UAS

Allele Dmel\jar^a.Scer\UAS