Allele Dmel\stan^E59

General Information
Symbol	Dmel\stan^E59	Species	D. melanogaster
Name		FlyBase ID	FBal0101421
Feature type	allele	Associated gene	Dmel\stan
Also Known As	fmi^E59

Map ( GBrowse )
Allele class	loss of function allele
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	loss of function allele (Usui et al., 1999)
Mutagen	ethyl methanesulfonate (Usui et al., 1999)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 2R:6,599,613..6,599,613 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=C6599613T pr_change=Q1837@\|stan-PA reported_pr_change=Q1838@ (Usui et al., 1999)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: Q1838@. Has a nonsense mutation in the ectodomain. (Usui et al., 1999)
Cytology
Phenotypic Data
Phenotypic Class
	body size defective \| larval stage (with Df(2R)stan2) (Bao et al., 2007) cell polarity defective \| somatic clone (Yang et al., 2002) decreased cell number \| somatic clone (Reuter et al., 2003) developmental rate defective \| larval stage (with Df(2R)stan2) (Bao et al., 2007) lethal \| embryonic stage \| recessive (Usui et al., 1999) lethal \| larval stage (with Df(2R)stan2) (Bao et al., 2007) locomotor behavior defective \| larval stage (with Df(2R)stan2) (Bao et al., 2007) neuroanatomy defective (Sweeney et al., 2002) neuroanatomy defective \| adult stage \| somatic clone (Hakeda-Suzuki et al., 2011) neuroanatomy defective \| larval stage (with Df(2R)stan2) (Bao et al., 2007) neuroanatomy defective \| larval stage (with stan⁷²) (Bao et al., 2007) neuroanatomy defective \| somatic clone (Sweeney et al., 2002, Reuter et al., 2003, Grueber et al., 2002, Bazigou et al., 2007) neuroanatomy defective \| third instar larval stage (with stan^E86) (Matsubara et al., 2011) partially lethal - majority die (with stan^E86) (Hakeda-Suzuki et al., 2011) planar polarity defective \| cell autonomous (Lu et al., 1999) planar polarity defective \| cell autonomous \| recessive \| somatic clone (Usui et al., 1999) planar polarity defective \| cell non-autonomous \| somatic clone (Das et al., 2002) planar polarity defective \| recessive (Usui et al., 1999) planar polarity defective \| somatic clone (Lawrence et al., 2004) planar polarity defective \| somatic clone (with stan^E45) (Strutt et al., 2002) visible (with stan^E45), with stan^Scer\UAS.cUa (Usui et al., 1999) visible \| cell autonomous \| recessive \| somatic clone (Usui et al., 1999)
Phenotype Manifest In
	2nd posterior cell & wing hair (Usui et al., 1999) axon & photoreceptor cell R8 \| somatic clone (Bazigou et al., 2007) dendrite \| dorsal \| embryonic stage (Gao et al., 1999) dendrite \| third instar larval stage (with stan^E86) (Matsubara et al., 2011) dendritic arborizing neuron \| somatic clone (Grueber et al., 2002) dorsal multidendritic neuron (Sweeney et al., 2002) dorsal multidendritic neuron \| somatic clone (Sweeney et al., 2002) dorsal multidendritic neuron \| third instar larval stage (with Df(2R)stan2) (Bao et al., 2007) dorsal multidendritic neuron ddaC \| somatic clone (Sweeney et al., 2002) dorsal multidendritic neuron ddaC \| third instar larval stage (with stan^E86) (Matsubara et al., 2011) dorsal multidendritic neuron ddaE \| somatic clone (Sweeney et al., 2002) dorsocentral bristle (Lu et al., 1999) embryonic/larval dorsal trunk (with stan¹⁹²) (Chung et al., 2009) embryonic/larval mushroom body \| somatic clone (Reuter et al., 2003) eye photoreceptor cell \| somatic clone (Das et al., 2002) eye photoreceptor cell \| somatic clone (with stan^E45) (Strutt et al., 2002) intersegmental nerve \| third instar larval stage (with Df(2R)stan2) (Bao et al., 2007) longitudinal connective (Usui et al., 1999) macrochaeta (Usui et al., 1999) microchaeta & scutum (Lu et al., 1999) multidendritic neuron (Sweeney et al., 2002) mushroom body calyx \| somatic clone (Reuter et al., 2003) neuromuscular junction (with Df(2R)stan2) (Bao et al., 2007) neuromuscular junction \| ectopic (with Df(2R)stan2) (Bao et al., 2007) neuromuscular junction \| ectopic (with stan⁷²) (Bao et al., 2007) ommatidium (Usui et al., 1999) ommatidium \| somatic clone (Yang et al., 2002, Das et al., 2002) ommatidium \| somatic clone (with stan^E45) (Strutt et al., 2002) photoreceptor cell & axon (Senti et al., 2003) photoreceptor cell R3 \| cell non-autonomous \| somatic clone (Das et al., 2002) photoreceptor cell R3 \| somatic clone (Yang et al., 2002) photoreceptor cell R4 \| cell non-autonomous \| somatic clone (Das et al., 2002) photoreceptor cell R4 \| somatic clone (Yang et al., 2002) photoreceptor cell R8 & axon (Senti et al., 2003) photoreceptor cell R8 & axon & growth cone (Senti et al., 2003) photoreceptor cell R8 \| somatic clone (Hakeda-Suzuki et al., 2011) presumptive embryonic/larval peripheral nervous system (Sweeney et al., 2002) trichome & abdominal tergite (with stan³) (Casal et al., 2006) trichome & pleural membrane \| somatic clone (Lawrence et al., 2004) wing cell \| P-stage (Classen et al., 2005) wing cell \| P-stage \| cell autonomous \| somatic clone (Classen et al., 2005) wing hair (Usui et al., 1999) wing hair \| somatic clone (Usui et al., 1999)
Detailed Description
	Statement Reference Axons of homozygous single cell R8 photoreceptor clones show targeting defects and stop abnormally at the M1 layer in the adult medulla. (Hakeda-Suzuki et al., 2011) stan[E86]/stan[E59] transheterozygotes survive until wandering third larval instar stages and even up to the adult stage. The branches of the ddaC class IV neurons show a four-fold increase in the crossing index within the dorsal region of the arbor in stan[E86]/stan[E59] third instar larvae compared to wild type, although the cumulative branch length of the dendrites is unchanged in the mutant larvae. The class IV neurons also show occasional axon misrouting and stalling of growth cones. (Matsubara et al., 2011) stan[192]/stan[E59] stage 16 embryos have longer dorsal trunks than normal. (Chung et al., 2009) Of the mutant larvae of the genotype stan[E59]/Df(2R)stan2, roughly 7.6% of the 1st instar larvae live to the third instar stage but die either at this stage or during pupation. Mutant larvae surviving to 3rd instar are smaller compared to similar aged controls. These animals do not grow into mature 3rd instar larvae for another 4-7 days and become slightly larger than wildtype wandering 3rd instar. Compared to wildtype, these mutant larvae are sluggish in locomotion either spontaneously or in response to a gentle poke. Their average speed is 0.14mm/s compared to 0.79mm/sec for controls. Approximately 27% of muscle 12 in stan[E59]/Df(2R)stan2, and 21% of stan[E59]/stan[72] larvae have ectopic type 1 synapses, compared to 1.5% in controls. Approximately 12.1% of stan[E59]/Df(2R)stan2 and 14.8% of stan[E59]/stan[72] larval muscle 13 have ectopic type 1 synapses. 73.3% of stan[E59]/Df(2R)stan2, and 70.5% of stan[E59]/stan[72] muscle 4 are ectopically innervated by type II motorneurons, compared to 15.4% of controls. Larval synapses of stan[E59]/Df(2R)stan2 and stan[E59]/stan[72] found on muscle 2, and to a lesser extent on muscle 3, exhibit abnormal, but functionally active synaptic bouton-like varicosities located along the intersegmental nerve tract. These varicosities are typically larger than wildtype synapses, and are found randomly at a low rate (3-5 per larvae) throughout all checked segments. 6.58% of segmental nerves from 3rd instar stan[E59]/Df(2R)stan2, and 6.75% of segmental nerves from 3rd instar stan[E59]/stan[72], show these axonal varicosities. 15.5% of muscle 12, 8.7% of muscle 13, and 7% of muscles 6/7 of 3rd instar stan[E59]/Df(2R)stan2 have completely lost their neuromuscular junctions. 8.6% of muscle 12, 4.7% of muscle 13, and 2% of muscles 6/7 of 3rd instar stan[E59]/stan[72] do not have any synaptic innervation. In stan[E59]/Df(2R)stan2 second instar larvae, 4.5% of muscle 12s observed show loss of synapses. Mutants display variable excitatory junction potential (EJP) defects, with some 3rd instar muscles showing a dramatic reduction in EJP and others showing no synaptic potentials when evoked by stimulation of the segmental nerve. Electron micrographs from segmental nerves of stan[E59]/Df(2R)stan2 show a reduced average diameter and cross-sectional area compared to controls. Gaps and signs of microtubule loss and axonal membrane breakup are seen in the mutants. Fewer axons are present in mutants (30.6) compared to controls (81.7). The peripheral glial sheath surrounding the segmental nerve is thicker in the mutant larvae compared to controls, and the average area of glial processes is significantly reduced. The ratio of glial area to nerve area was reduced from 0.49 in controls to 0.33 in stan[E59]/Df(2R)stan2. The average area of the segmental nerve of stan[E59]/Df(2R)stan2 animals, in which stan[Scer\UAS.cUa] is driven by Scer\GAL4[elav-C155], is significantly larger than in wildtype controls or in the stan[E59]/Df(2R)stan2 mutants. A small number of axons (2-3 per segmental nerve) axons have large vacuoles. The rescued segmental nerves contain more axons (113) than the mutants or the wildtype controls, and show a rescue of the thickness of the peripheral glial sheath surrounding the segmental nerve compared to mutants. The average number of sensory cells (multidendritic neurons and external sensory cells) in the dorsal cluster is 10.97 in stan[E59]/Df(2R)stan2, compared to 9.89 in controls, which, although small, is statistically significant. (Bao et al., 2007) Clones of stan^E59 in the lamina target neurons result in R8 axon guidance defects. (Bazigou et al., 2007) stan³/stan^E59 flies have almost normal hair polarities in the tergite, apart from near the front and near the rear of the anterior compartment. (Casal et al., 2006) The hexagonal packing of intervein cells, which usually occurs between wing development stage P2B (when the first morphological signs of veins appear (FBrf0005070), and the middle of P2C (before hair formation (FBrf0005070)) is disrupted in stan^E59/stan^E59 flies. This phenotype is also seen in somatic clones of stan^E59/stan^E59 cells in the developing intervein, but this effect is cell-autonomous. (Classen et al., 2005) Large somatic clones of stan^E59 homozygous cells in the wing have largely normal planar polarity. However, when these clones fall in the ventral pleura of the abdomen they have a disheveled planar polarity phenotype. These clones show occasional local non-autonomy, disturbing the polarity of hairs here and there in front of the clone. (Lawrence et al., 2004) Mutant mushroom body neuroblast clones have a significant reduction in cell number compared to control clones and overextend processes from the mushroom body calyx. Overextended processes from the calyx are also seen in the adult. (Reuter et al., 2003) When stan^E59 clones are made in the developing eye, R axons terminate in a highly disorganised pattern, when compared to wild-type, particularly within the medulla. R1-R6 axons correctly target the lamina, and R7 axons generally appear to select their correct target although their termini are slightly disorganised. When single cell clones are made in R7 axons, they always target normally. However R8 axons are highly disorganised and although they initially reach their correct target layer in the medulla it appears they later retract to more superficial layers. The R8 growth cones are irregularly spaced and have a 'club-like' morphology, but they have many elaborate fine processes. the processes of individual R8 growth cones often overlap intensively. Despite this irregular spacing, the entire target field appears to be filled and there does not appear to be any dramatic misrouting of axons within the optic lobe. The ommatidial fascicles within the optic stalk are basically wild-type though there is a 5% increase in the number of fascicles comprising more than 8 R axons. (Senti et al., 2003) Mutants show no significant disruption of ovarian morphology. (Cohen et al., 2002) Somatic clones of stan^E59 in the eye display defects in ommatidial chirality (random chirality and symmetrical clusters) and rotation. In these clones, apical-basal polarity, cell subtype specification and cell survival all appear to be normal, but approximately 20% of ommatidia have reduced numbers of photoreceptors. In mosaic ommatidia, chirality of the whole ommatidium is dependent on the genotype of R3 and R4 photoreceptors: If R3 and R4 are wild-type then ommatidial chirality is normal. If either or both fall within a stan^E59 somatic clone then the ommatidium has random chirality or stays symmetrical. (Das et al., 2002) Most single-cell clone stan^E59 da neurons terminate their dendritic fields at normal locations at the lateral margin and segment border (where they meet or approach the like dendrites of adjacent neurons). However, in a few cases, the mutant neurons have sparsely branched processes which extend beyond these territories. (Grueber et al., 2002) 36.8% of ommatidia in stan^E45/stan^E59 mutant somatic clones in the eye are normal. 20.2% have rotated ommatidia, 26.1% have chirality defects, 15.9% are achiral (1% unscorable). (Strutt et al., 2002) Single homozygous dorsal cluster multiple dendritic (MD) neurons (in an otherwise wild-type background) overextend one or more dendritic processes towards the dorsal midline in about 15% of cases. The basic architecture of the dendritic branching pattern is not altered. 10% of single mutant MD neurons (in an otherwise wild-type background) do not extend their axons fully to the central nervous system. Multiple dendritic (MD) neuron dorsal dendritic processes have already extended in 74% of hemisegments in homozygous embryos 11-12 hours after egg laying (in contrast to wild-type embryos where the dorsal dendrites of the MD neurons have not yet extended at this stage). Axonal break points are seen in the axon bundles of dorsal cluster MD neurons. The axons of the PNS neurons fail to fasciculate as tightly as in wild type. (Sweeney et al., 2002) Homozygous mutant somatic clones in the eye, lead to dramatic polarity defects. These include aberrant rotation and a lack of distinct R3/R4 fates. (Yang et al., 2002) When trans-heterozygous with stan⁷², exhibits excessive dorsal branch outgrowth and misrouting in the dorsal cluster of embryonic dendrites. (Gao et al., 1999) The bristles on the notum of stan^E59/stan⁷¹ flies show disorganised polarity and point towards different directions. Over 50% of the posterior, left lateral and right lateral bristles showed polarity defects, whereas only about 10% of the anterior and dorsocentral bristles showed defects. Homozygous stan^E59 clones on the notum show disrupted planar polarity of bristles in a cell autonomous fashion, with cells outside the clone only rarely being affected. (Lu et al., 1999) Mutants show local disconnection of longitudinal axon fascicles in the central nervous system. stan^E45/stan^E59 mutants partially rescued by stan^Scer\UAS.cUa driven by Scer\GAL4¹⁴⁰⁷ have planar polarity defects in ommatidia, sensory bristles and wing hairs. In region D (2nd posterior cell) of the wing, hairs are deflected from the proximo-distal axis and oriented towards the posterior wing margin. In somatic clones in the wing, polarity disruption of wing hairs is seen in a cell autonomous fashion. Prehairs emerge near the cell centre, or at the wrong locations along the cell periphery. Very occasionally, twin hairs were seen in wild-type cells bordering the clone. (Usui et al., 1999)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference stan^E86/stan^E59 has partially lethal - majority die phenotype, enhanceable by gogo^D869 (Hakeda-Suzuki et al., 2011) stan^E86/stan^E59 has partially lethal - majority die phenotype, enhanceable by gogo^H1675 (Hakeda-Suzuki et al., 2011)
Suppressed by
Statement Reference pwn^unspecified, stan^E59 has planar polarity defective \| somatic clone \| cell non-autonomous phenotype, suppressible \| somatic clone by fz^Scer\UAS.cSa/Scer\GAL4^αTub84B.PL (Lawrence et al., 2004)
Enhancer of
Statement Reference stan^E59 is an enhancer of cell polarity defective phenotype of S^48-5 (Gaengel and Mlodzik, 2003) stan^E59 is an enhancer of cell polarity defective phenotype of Scer\GAL4^hs.2sev, pk^{sple.Scer\UAS} (Jenny et al., 2003) stan^E59 is an enhancer of cell polarity defective phenotype of Scer\GAL4^hs.2sev, Vang^Scer\UAS.cWa (Jenny et al., 2003) stan^E59 is an enhancer of planar polarity defective phenotype of Vang^sev.PR (Rawls and Wolff, 2003) stan^E59 is an enhancer of visible phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, btl^{Scer\UAS.T:λ\cI-DD}, stumps^{Scer\UAS.T:Zzzz\FLAG} (Zhu et al., 2005)
NOT Enhancer of
Statement Reference stan[+]/stan^E59 is a non-enhancer of cell polarity defective phenotype of Scer\GAL4^hs.2sev, shg^{dCR3h.Scer\UAS.T:Avic\GFP-rs} (Mirkovic and Mlodzik, 2006) stan[+]/stan^E59 is a non-enhancer of planar polarity defective phenotype of Scer\GAL4^hs.2sev, nmo^Scer\UAS.cUa (Mirkovic et al., 2011) stan[+]/stan^E59 is a non-enhancer of visible phenotype of Scer\GAL4^en-e16E, kermit^GS2053 (Djiane and Mlodzik, 2010) stan^E59 is a non-enhancer of cell polarity defective phenotype of pk^pk.sev (Jenny et al., 2003)
Suppressor of
Statement Reference stan[+]/stan^E59 is a suppressor of planar polarity defective phenotype of Scer\GAL4^hs.2sev, dgo^Scer\UAS.cFa (Weber et al., 2012) stan[+]/stan^E59 is a suppressor of visible phenotype of Scer\GAL4^hs.2sev, dgo^Scer\UAS.cFa (Weber et al., 2012) stan^E59/stan³ is a suppressor of planar polarity defective phenotype of Scer\GAL4^ptc-559.1, fz^Scer\UAS.cSa (Lawrence et al., 2004) stan^E59/stan^E59 is a suppressor of planar polarity defective \| somatic clone \| cell non-autonomous phenotype of Scer\GAL4^αTub84B.PL, Vang^Scer\UAS.cWa (Lawrence et al., 2004, Lawrence et al., 2004)
NOT Suppressor of
Statement Reference stan[+]/stan^E59 is a non-suppressor of cell polarity defective phenotype of Scer\GAL4^hs.2sev, shg^{dCR3h.Scer\UAS.T:Avic\GFP-rs} (Mirkovic and Mlodzik, 2006) stan[+]/stan^E59 is a non-suppressor of planar polarity defective phenotype of Scer\GAL4^hs.2sev, nmo^Scer\UAS.cUa (Mirkovic et al., 2011) stan[+]/stan^E59 is a non-suppressor of visible phenotype of Scer\GAL4^en-e16E, kermit^GS2053 (Djiane and Mlodzik, 2010) stan^E59 is a non-suppressor of cell polarity defective phenotype of pk^pk.sev (Jenny et al., 2003)
Other
Statement Reference esn^KO6, stan[+]/stan^E59 has neuroanatomy defective \| third instar larval stage phenotype (Matsubara et al., 2011) fry¹, stan[+]/stan^E59 has neuroanatomy defective \| third instar larval stage phenotype (Matsubara et al., 2011) fry⁰²²⁴⁰, stan[+]/stan^E59 has neuroanatomy defective \| third instar larval stage phenotype (Matsubara et al., 2011) pwn^unspecified, stan^E59 has planar polarity defective \| somatic clone phenotype (Lawrence et al., 2004) Rho1^E3.10, stan[+]/stan^E59 has neuroanatomy defective \| third instar larval stage phenotype (Matsubara et al., 2011) stan[+]/stan^E59, trc¹ has neuroanatomy defective \| third instar larval stage phenotype (Matsubara et al., 2011) stan[+]/stan^E59, trc² has neuroanatomy defective \| third instar larval stage phenotype (Matsubara et al., 2011) Vang^A3, stan[+]/stan^E59 has neuroanatomy defective \| third instar larval stage phenotype (Matsubara et al., 2011) Vang^stbm-6, stan[+]/stan^E59 has neuroanatomy defective \| third instar larval stage phenotype (Matsubara et al., 2011)
Phenotype Manifest In
Suppressed by
Statement Reference pwn^unspecified, stan^E59 has trichome & adult abdomen \| somatic clone \| cell non-autonomous phenotype, suppressible \| somatic clone by fz^Scer\UAS.cSa/Scer\GAL4^αTub84B.PL (Lawrence et al., 2004)
Enhancer of
Statement Reference stan^E59 is an enhancer of eye phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, btl^{Scer\UAS.T:λ\cI-DD}, stumps^{Scer\UAS.T:Zzzz\FLAG} (Zhu et al., 2005) stan^E59 is an enhancer of ommatidium phenotype of S^48-5 (Gaengel and Mlodzik, 2003) stan^E59 is an enhancer of ommatidium phenotype of Scer\GAL4^hs.2sev, pk^{sple.Scer\UAS} (Jenny et al., 2003) stan^E59 is an enhancer of ommatidium phenotype of Scer\GAL4^hs.2sev, Vang^Scer\UAS.cWa (Jenny et al., 2003) stan^E59 is an enhancer of ommatidium phenotype of Vang^sev.PR (Rawls and Wolff, 2003)
NOT Enhancer of
Statement Reference stan[+]/stan^E59 is a non-enhancer of ommatidium phenotype of Scer\GAL4^hs.2sev, nmo^Scer\UAS.cUa (Mirkovic et al., 2011) stan[+]/stan^E59 is a non-enhancer of ommatidium phenotype of Scer\GAL4^hs.2sev, shg^{dCR3h.Scer\UAS.T:Avic\GFP-rs} (Mirkovic and Mlodzik, 2006) stan[+]/stan^E59 is a non-enhancer of wing hair phenotype of Scer\GAL4^en-e16E, kermit^GS2053 (Djiane and Mlodzik, 2010) stan^E59 is a non-enhancer of ommatidium phenotype of pk^pk.sev (Jenny et al., 2003)
Suppressor of
Statement Reference stan[+]/stan^E59 is a suppressor of wing hair phenotype of Scer\GAL4^hs.2sev, dgo^Scer\UAS.cFa (Weber et al., 2012) stan^E59/stan³ is a suppressor of trichome & adult abdomen phenotype of Scer\GAL4^ptc-559.1, fz^Scer\UAS.cSa (Lawrence et al., 2004) stan^E59/stan^E59 is a suppressor of trichome & adult abdomen \| somatic clone \| cell non-autonomous phenotype of Scer\GAL4^αTub84B.PL, Vang^Scer\UAS.cWa (Lawrence et al., 2004, Lawrence et al., 2004)
NOT Suppressor of
Statement Reference stan[+]/stan^E59 is a non-suppressor of ommatidium phenotype of Scer\GAL4^hs.2sev, nmo^Scer\UAS.cUa (Mirkovic et al., 2011) stan[+]/stan^E59 is a non-suppressor of ommatidium phenotype of Scer\GAL4^hs.2sev, shg^{dCR3h.Scer\UAS.T:Avic\GFP-rs} (Mirkovic and Mlodzik, 2006) stan[+]/stan^E59 is a non-suppressor of wing hair phenotype of Scer\GAL4^en-e16E, kermit^GS2053 (Djiane and Mlodzik, 2010) stan^E59 is a non-suppressor of ommatidium phenotype of pk^pk.sev (Jenny et al., 2003)
Other
Statement Reference esn^KO6, stan[+]/stan^E59 has dendrite \| third instar larval stage phenotype (Matsubara et al., 2011) esn^KO6, stan[+]/stan^E59 has dorsal multidendritic neuron ddaC \| third instar larval stage phenotype (Matsubara et al., 2011) fry¹, stan[+]/stan^E59 has dendrite \| third instar larval stage phenotype (Matsubara et al., 2011) fry¹, stan[+]/stan^E59 has dorsal multidendritic neuron ddaC \| third instar larval stage phenotype (Matsubara et al., 2011) fry⁰²²⁴⁰, stan[+]/stan^E59 has dendrite \| third instar larval stage phenotype (Matsubara et al., 2011) fry⁰²²⁴⁰, stan[+]/stan^E59 has dorsal multidendritic neuron ddaC \| third instar larval stage phenotype (Matsubara et al., 2011) pwn^unspecified, stan^E59 has trichome & adult abdomen \| somatic clone phenotype (Lawrence et al., 2004) Rho1^E3.10, stan[+]/stan^E59 has dendrite \| third instar larval stage phenotype (Matsubara et al., 2011) Rho1^E3.10, stan[+]/stan^E59 has dorsal multidendritic neuron ddaC \| third instar larval stage phenotype (Matsubara et al., 2011) stan[+]/stan^E59, trc¹ has dendrite \| third instar larval stage phenotype (Matsubara et al., 2011) stan[+]/stan^E59, trc¹ has dorsal multidendritic neuron ddaC \| third instar larval stage phenotype (Matsubara et al., 2011) stan[+]/stan^E59, trc² has dendrite \| third instar larval stage phenotype (Matsubara et al., 2011) stan[+]/stan^E59, trc² has dorsal multidendritic neuron ddaC \| third instar larval stage phenotype (Matsubara et al., 2011) Vang^A3, stan[+]/stan^E59 has dendrite \| third instar larval stage phenotype (Matsubara et al., 2011) Vang^A3, stan[+]/stan^E59 has dorsal multidendritic neuron ddaC \| third instar larval stage phenotype (Matsubara et al., 2011) Vang^stbm-6, stan[+]/stan^E59 has dendrite \| third instar larval stage phenotype (Matsubara et al., 2011) Vang^stbm-6, stan[+]/stan^E59 has dorsal multidendritic neuron ddaC \| third instar larval stage phenotype (Matsubara et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference stan[E59]/+ significantly suppresses the wing defects induced by the overexpression of dgo[Scer\UAS.cFa] under the control of Scer\GAL4[hs.2sev]. (Weber et al., 2012) stan[E59] shows increased crossing of dendrites in the dendritic arbor of the ddaC class IV neurons compared to wild type in double heterozygous combination with each of esn[KO6], Rho1[E3.10], trc[1], trc[2], fry[1], fry[02240], Vang[stbm-6] or Vang[A3] (none of the single heterozygotes show this phenotype). (Matsubara et al., 2011) The reversal of cell polarity that is seen in cells posterior to clones in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) where the clones are expressing ds^{ecto.Scer\UAS} under the control of Scer\GAL4^αTub84B.PL is still seen if the clones are induced in a stan^- background (the cells within the clone are stan^E59/stan^E59 while the cells surrounding the clone are stan³/stan^E59). Clones of stan^E59 fz^unspecified double mutant cells in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) do not change the cell polarity of their wild-type neighbouring cells. Clones of cells in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) that are expressing fz^Scer\UAS.cUa under the control of Scer\GAL4^αTub84B.PL in a stan^- background (the cells within the clone are stan^E59/stan^E59 while the cells surrounding the clone are stan³/stan^E59) do not change the cell polarity of the stan³/stan^E59 cells surrounding the clone. Clones of cells in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) that are co-expressing stan^Scer\UAS.cUa and fz^Scer\UAS.cUa under the control of Scer\GAL4^αTub84B.PL in a stan^- background (the cells within the clone are stan^E59/stan^E59 while the cells surrounding the clone are stan³/stan^E59) do not change the cell polarity of the stan³/stan^E59 cells surrounding the clone. The reversal of cell polarity that is seen in cells anterior to clones in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) where the clones are expressing ft^Scer\UAS.cMa under the control of Scer\GAL4^αTub84B.PL is still seen if the clones are induced in a stan^- background (the cells within the clone are stan^E59/stan^E59 while the cells surrounding the clone are stan³/stan^E59). Clones of cells in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) that are expressing fj^Scer\UAS.cZa under the control of Scer\GAL4^αTub84B.PL in a stan^- background (the cells within the clone are stan^E59/stan^E59 while the cells surrounding the clone are stan³/stan^E59) result in a reversal of cell polarity in cells anterior to the clone (hairs in front of the clone point forwards), as occurs in clones induced in a wild-type background. The reversal of cell polarity that is seen in cells posterior to clones in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) where the clones are expressing ds^{ecto.Scer\UAS} under the control of Scer\GAL4^αTub84B.PL is still seen if the clones are induced in a fz^unspecified stan^- background (cells within the clone are stan^E59/stan^E59 while the cells surrounding the clone are stan³/stan^E59). The reversal of cell polarity that is seen in cells anterior to clones in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) where the clones are expressing ft^Scer\UAS.cMa under the control of Scer\GAL4^αTub84B.PL is still seen if the clones are induced in a fz^unspecified stan^- background (cells within the clone are stan^E59/stan^E59 while the cells surrounding the clone are stan³/stan^E59). Hair and bristle polarity is randomised throughout the tergite in ds^unspecified stan³/stan^E59 double mutant flies. The reversal of cell polarity phenotype that is seen in cells posterior to clones in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) where the clones are doubly mutant for ptc¹⁶ and Df(2R)en^E is still seen if the clones are induced in a stan³/stan^E59 background, as occurs in clones induced a wild-type background. The reversal of cell polarity phenotype that is seen in cells posterior to clones in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) where the clones are doubly mutant for ptc¹⁶ and Df(2R)en^E is not seen if the clones are induced in a ds^unspecified stan³/stan^E59 double mutant background instead of a wild-type background. Clones of cells in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) where the clones are triply mutant for ptc¹⁶, Df(2R)en^E and stan^E59 and have been induced in a ds^unspecified background do not result in a reversal of cell polarity in cells surrounding the clone. (Casal et al., 2006) A stan^E59/+ background does not affect the Scer\GAL4^hs.2sev>shg^{dCR3h.Scer\UAS.T:Avic\GFP-rs} ommatidial phenotype. (Mirkovic and Mlodzik, 2006) In wings largely made up of pwn^unspecified stan^E59 homozygous somatic clones, the remaining heterozygous cells have normal polarity. pwn^unspecified stan^E59 homozygous somatic clones in the abdomen have a disheveled planar polarity phenotype. These clones occasionally cause non-autonomous effects on planar polarity just outside and usually anterior to the clone. This limited non-autonomous phenotype is suppressed if the clones are also fz^Scer\UAS.cSa; Scer\GAL4^αTub84B.PL (produced by Scer\GAL80^αTub84B.PL MARCM method). The non-autonomous effect on planar polarity of Vang^Scer\UAS.cWa; Scer\GAL4^αTub84B.PL somatic clones (produced by the Scer\GAL80 MARCM method) in the adult abdomen is suppressed by pwn/pwn stan^E59/stan^E59. Cells within the resulting clones have randomised planar polarity. The planar repolarisation seen in the adult abdomen of fz^Scer\UAS.cSa; Scer\GAL4^ptc-559.1 adults is suppressed by stan^E59/stan³. (Lawrence et al., 2004) S^48-5/stan^E59 mutants show 45.59%+-7.32 misrotated ommatidia compared to 9.55%+-1.43 seen in S^48-5 mutants alone. (Gaengel and Mlodzik, 2003)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	stan^C512 (Senti et al., 2003) stan^G233 (Senti et al., 2003) stan^I452 (Senti et al., 2003) stan^I577 (Senti et al., 2003) stan^K1399 (Senti et al., 2003) stan^MB (Reuter et al., 2003) stan^R1456 (Senti et al., 2003) stan^R2151 (Senti et al., 2003) stan^R2799 (Senti et al., 2003) stan^S826 (Senti et al., 2003)
Rescued by	Df(2R)stan2/stan^E59 is rescued by Scer\GAL4^elav-C155/stan^Scer\UAS.cUa (Bao et al., 2007) stan⁷²/stan^E59 is rescued by stan^Scer\UAS.cUa (Bao et al., 2007) stan^E59 is rescued by Scer\GAL4^ey-OK107/stan^Scer\UAS.cUa (Reuter et al., 2003) stan^E59 is rescued by stan^GMR.PS (Senti et al., 2003) stan^E59 is rescued by stan^Scer\UAS.cUa/Scer\GAL4^GMR.PU (Hakeda-Suzuki et al., 2011) stan^E59 is rescued by stan^{ΔIntra.Scer\UAS}/Scer\GAL4^GMR.PU (Hakeda-Suzuki et al., 2011) stan^E86/stan^E59 is rescued by stan^Scer\UAS.AEY/Scer\GAL4⁴⁷⁷ (Matsubara et al., 2011) stan^E86/stan^E59 is rescued by stan^Scer\UAS.TTV/Scer\GAL4⁴⁷⁷ (Matsubara et al., 2011)
Partially rescued by	stan⁷²/stan^E59 is partially rescued by stan^Scer\UAS.cUa (Bao et al., 2007) stan⁷²/stan^E59 is partially rescued by stan^{ΔEX.Scer\UAS} (Bao et al., 2007) stan^E45/stan^E59 is partially rescued by stan^Scer\UAS.cUa/Scer\GAL4¹⁴⁰⁷ (Usui et al., 1999)
Not rescued by	stan⁷²/stan^E59 is not rescued by stan^{ΔEX.Scer\UAS} (Bao et al., 2007) stan^E86/stan^E59 is not rescued by stan^{ΔJM-A.Scer\UAS.TTV}/Scer\GAL4⁴⁷⁷ (Matsubara et al., 2011)
Comments	Expression of either stan[Scer\UAS.cUa] or stan[ΔIntra.Scer\UAS] under the control of Scer\GAL4[GMR.PU] rescues the axon targeting defects of homozygous single cell stan[E59] R8 photoreceptor clones. (Hakeda-Suzuki et al., 2011) Expression of stan[Scer\UAS.TTV] or of stan[Scer\UAS.AEY] under the control of Scer\GAL4[477] rescues the increased crossing that is seen in the dorsal dendritic arbor of the ddaC class IV neurons in stan[E86]/stan[E59] third instar larvae. Expression of stan[ΔJM-A.Scer\UAS.TTV] under the control of Scer\GAL4[477] does not rescue the increased crossing that is seen in the dorsal dendritic arbor of the ddaC class IV neurons in stan[E86]/stan[E59] third instar larvae. (Matsubara et al., 2011) The lethality and axonal defects seen in stan^E45/stan^E59 can be rescued by stan^Scer\UAS.cUa driven by Scer\GAL4¹⁴⁰⁷. This combination does not affect the planar polarity phenotype. (Usui et al., 1999)
Stocks ( 1 )
Bloomington	41776 w^*; P{neoFRT}42D stan^E59/CyO
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 8 )
Reported As
Symbol Synonym	fam^E59 (Das et al., 2004) flamingo^E59 (Sweeney et al., 2002) fmi⁵⁹ (Axelrod, 2001) fmi^E59 (Zhu et al., 2005, Wu et al., 2004, Zhu and Luo, 2004, Gaengel and Mlodzik, 2003, Jenny et al., 2003, Bastock et al., 2003, Rawls and Wolff, 2003, Senti et al., 2003, Das et al., 2002, Reuter et al., 2003, Grueber et al., 2002, Strutt et al., 2002, Cohen et al., 2002, Yang et al., 2002, Gao et al., 2000, Lu et al., 1999, Gao et al., 1999, Usui et al., 1999, Strutt et al., 2006, Bazigou et al., 2007, Mirkovic and Mlodzik, 2006, Bazigou et al., 2007, Classen et al., 2005, Mirkovic et al., 2011, Hakeda-Suzuki et al., 2011, Gontang et al., 2011, Senti et al., 2003, Bao et al., 2007, Chung et al., 2009, Djiane and Mlodzik, 2010, Hakeda-Suzuki et al., 2011, Matsubara et al., 2011) fmi-E59 (Gaengel and Mlodzik, 2003) fmi e59 (Chen and Clandinin, 2008) stan^E59 (Lawrence et al., 2004, Casal et al., 2006, Weber et al., 2012)
Name Synonym	flamingo^e59 (Gontang et al., 2011) flamingo^E59 (Gontang et al., 2011)
Secondary FlyBase IDs

References ( 40 )

Generate a list of	All references relating to this allele ( 40 )

List References by type	Research paper ( 36 ) Supplementary material ( 4 )
Recent research papers ( 4 )
	Weber et al., 2012, Genetics 191(1): 145--162 Novel regulators of planar cell polarity: a genetic analysis in Drosophila. [FBrf0218210] Gontang et al., 2011, Development 138(22): 4899--4909 The cytoskeletal regulator Genghis khan is required for columnar target specificity in the Drosophila visual system. [FBrf0216508] Hakeda-Suzuki et al., 2011, Nat. Neurosci. 14(3): 314--323 Golden Goal collaborates with Flamingo in conferring synaptic-layer specificity in the visual system. [FBrf0213128] Matsubara et al., 2011, Genes Dev. 25(18): 1982--1996 The seven-pass transmembrane cadherin Flamingo controls dendritic self-avoidance via its binding to a LIM domain protein, Espinas, in Drosophila sensory neurons. [FBrf0215818] Show all research papers ...

Allele Dmel\stanE59

Allele Dmel\stan^E59