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General Information
Symbol
Dmel\Rho1UAS.cMa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Mlodzik
FlyBase ID
FBal0101563
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Rho1, UAS-RhoA, UASrho1, UAS-Rho1+
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS sequences drive wild-type Rho1.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expressing Rho1Scer\UAS.cMa for 12 hours under the control of Scer\GAL4P0.5.Pdf (restricted to the adult stages using Scer\GAL80ts.αTub84B) significantly reduces the spread of s-LNv neuron projections usually seen at ZT24 (dawn). This dusk-like retracted state is still observed 2.5 days after induction. When Rho1Scer\UAS.cMa is expressed for 12 hours starting at ZT24 (dawn), a normal spread of projections is seen at ZT12 (dusk).

Expression of Rho1Scer\UAS.cMa under the control of Scer\GAL4P0.5.Pdf (restricted to the adult stages using Scer\GAL80ts.αTub84B) suppresses the activity-dependent spreading of s-LNVs seen in controls. In controls, three hours of neuron activation beginning when projections are retracted at dusk (ZT12) results in increases in spread and axonal volume similar to those normally seen at dawn (ZT24). This increase in spread is not observed upon co-expression of Rho1Scer\UAS.cMa. The usual increase in clock protein levels seen upon LNv firing is also disrupted by Rho1Scer\UAS.cMa.

Flies expressing Rho1Scer\UAS.cMa under the control of Scer\GAL4P0.5.Pdf (restricted to the adult stages using Scer\GAL80ts.αTub84B) are either arrhythmic or weakly rhythmic in complete darkness (DD). The phase of clock proteins in DN1 clock neurons is shifted by ~6 hours between control and Rho1Scer\UAS.cMa-induced flies after three days in DD.

In contrast to controls, which adjust their morning and evening activity peaks to align with seasonal dawn and dusk, flies expressing Rho1Scer\UAS.cMa under the control of Scer\GAL4P0.5.Pdf (restricted to the adult stages using Scer\GAL80ts.αTub84B) are active prior to dawn when exposed to a winter light:dark cycle (10 hours day:14 hours night). Timing of activity is unaffected compared to controls when the flies are exposed to a summer cycle (14 hours day:10 hours night).

Expressing Rho1Scer\UAS.cMa for 14 hours under the control of Scer\GAL4P0.5.Pdf (restricted to the adult stages using Scer\GAL80ts.αTub84B) reduces the number of active zones in LNv projections at ZT2 to levels normally seen in controls at ZT14. The normally low number of active zones seen at ZT14 in controls are unaffected by Rho1Scer\UAS.cMa.

Expression of Rho1Scer\UAS.cMa in a stripe of cells at the anterior/posterior boundary of the larval wing disc under the control of Scer\GAL4ptc-559.1 results in cell migration.

Scer\GAL4GMR.PF-mediated expression of Rho1Scer\UAS.cMa results in a rough eye.

Expression of Rho1Scer\UAS.cMa under the control of Scer\GAL4dpp.blk1 induces ectopic apoptosis in third instar wing imaginal disc cells.

Expression of Rho1Scer\UAS.cMa under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 does not block germ cell transepithelial migration or later germ cell migration.

In Rho1Scer\UAS.cMa; Scer\GAL4ptc-559.1 larvae, cells immediately anterior to the anterior-compartment boundary lose their apical profiles, delaminate from the epithelium and show increased levels of apoptosis.

When Rho1Scer\UAS.cMa is driven by Scer\GAL4GMR.PF a rough eye phenotype is seen.

Expression of Rho1Scer\UAS.cMa under the control of Scer\GAL4OK107 does not result in axon growth defects in the mushroom body.

Expression of Rho1Scer\UAS.cMa by Scer\GAL4nos.UTR.T:Hsim\VP16 has no effect on germ cell migration.

Cells expressing Rho1Scer\UAS.cMa under the control of Scer\GAL4Bx-MS1096 in the wing disc mislocalise F-actin and lose epithelial characteristics. Cells lacking apical-basal polarity accumulate basal to the epithelial layer in these discs. Cells in the wing disc marked by Avic\GFP expression and expressing Rho1Scer\UAS.cMa under the control of Scer\GAL4ptc-559.1 disperse from their normal position and can invade adjacent regions of the disc.

Rho1Scer\UAS.cMa driven by Scer\GAL4prd.RG1 has no effect on cytokinesis or mitosis.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
Enhancer of
Suppressor of
NOT Suppressor of
Phenotype Manifest In
Enhanced by
Statement
Reference
Suppressed by
NOT suppressed by
Enhancer of
Suppressor of
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

Expression of Rho1Scer\UAS.cMa suppresses the shortening of the behavioural rhythm period seen when sggEP1576 is expressed under the control of Scer\GAL4P0.5.Pdf (limited to the adult stages using Scer\GAL80ts.αTub84B)in complete darkness (DD).

Co-expression of MbsScer\UAS.cLa suppresses the reduction in axonal volume and spread of s-LNv neuron projections seen at ZT24 (dawn), following 12 hours of expression of Rho1Scer\UAS.cMa under the control of Scer\GAL4P0.5.Pdf (restricted to the adult stages using Scer\GAL80ts.αTub84B).

The behavioural arrhythmicity of flies expressing Rho1Scer\UAS.cMa under the control of Scer\GAL4P0.5.Pdf from birth is rescued upon co-expression of MbsScer\UAS.cLa.

The behavioural arrhythmicity of flies overexpressing Rho1Scer\UAS.cMa under the control of Scer\GAL4P0.5.Pdf from birth is rescued is rescued upon co-expression of PuraGD12260.

The behavioural arrhythmicity of flies overexpressing Rho1Scer\UAS.cMa under the control of Scer\GAL4P0.5.Pdf from birth is rescued is rescued upon co-expression of PuradsRNA.shRNA.Scer\UAS.

Co-expression of PuraGD12260 suppresses the reduction in axonal volume and spread of s-LNv neuron projections seen at ZT24 (dawn), following 12 hours of expression of Rho1Scer\UAS.cMa under the control of Scer\GAL4P0.5.Pdf (restricted to the adult stages using Scer\GAL80ts.αTub84B).

A tsrN96A/+ background suppresses the cell migration seen upon expression of Rho1Scer\UAS.cMa under the control of Scer\GAL4ptc-559.1.

An ena23/+ or ena210/+ background suppresses the cell migration seen upon expression of Rho1Scer\UAS.cMa under the control of Scer\GAL4ptc-559.1.

Co-expression of conuScer\UAS.T:Uuuu\Myr5,T:Zzzz\FLAG, but not conuR402A.Scer\UAS.T:Uuuu\Myr5,T:Zzzz\FLAG, strongly suppresses the rough eye of Scer\GAL4GMR.PF Rho1Scer\UAS.cMa flies.

Co-expression of conuGAP.Scer\UAS.T:Uuuu\Myr5,T:Zzzz\FLAG strongly suppresses the rough eye of Scer\GAL4GMR.PF Rho1Scer\UAS.cMa flies.

Co-expression of bskDN.Scer\UAS suppresses the ectopic apoptosis in cells expressing Rho1Scer\UAS.cMa under the control of Scer\GAL4dpp.blk1.

Co-expression of pucScer\UAS.P\T.T:Hsap\MYC strongly suppresses the ectopic apoptosis in cells expressing Rho1Scer\UAS.cMa under the control of Scer\GAL4dpp.blk1.

Co-expression of hepdsRNA.Scer\UAS suppresses the ectopic apoptosis in cells expressing Rho1Scer\UAS.cMa under the control of Scer\GAL4dpp.blk1.

Co-expression of both slprdsRNA.Scer\UAS.NIG and Tak1dsRNA.Scer\UAS significantly suppresses the ectopic apoptosis in cells also expressing Rho1Scer\UAS.cMa under the control of Scer\GAL4dpp.blk1.

Heterozygous pucE69 mutant flies expressing Rho1Scer\UAS.cMa under the control of Scer\GAL4dpp.blk1 exhibit a severely reduced or rudimentary wing phenotype.

Expression of Rho1Scer\UAS.cMa in the germline under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 does not rescue the germ cell death seen in embryos derived from females carrying wun49 wun2EP2650ex34 germline clones.

Co-expression of Rho1Scer\UAS.cMa does not enhance the partial rescue of pbl3 mesodermal migration defects seen when pblΔBRCT.Scer\UAS.T:Hsap\MYC is expressed under the control of Scer\GAL4twi.2PE.

The multiple wing hair phenotype caused by expression of towScer\UAS.cCa under the control of Scer\GAL4hs.PB using heat shock at 24 hours after puparium formation is partially suppressed by co-expression of Rho1Scer\UAS.cMa.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescues
Comments

Rho172O clones are rescued by expression of Rho1Scer\UAS.cMa. In some cases, a decrease in the apical area is observed, perhaps due to the high level of Rho1Scer\UAS.cMa overexpression.

Rho172F clones are rescued by expression of Rho1Scer\UAS.cMa. In some cases, a decrease in the apical area is observed, perhaps due to the high level of Rho1Scer\UAS.cMa overexpression.

Expression of Rho1Scer\UAS.cMa specifically in the hemocytes (using the Scer\GAL4crq.PA driver) resuces the hemocyte recruitment defect seen in Rho11B mutants.

Rho1Scer\UAS.cMa partially rescues the phenotype of homozygous Rho172R mushroom body neuroblast clones when expressed under the control of Scer\GAL4elav-C155. Rho1Scer\UAS.cMa rescues the dendrite phenotype of homozygous Rho172O mushroom body neuroblast clones when expressed under the control of Scer\GAL4Tab2-201Y, without altering the proliferation defect.

Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Rho1Scer\UAS.cLa
Rho1Scer\UAS.cMa
Rho1UAS.cMa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Mlodzik
Secondary FlyBase IDs
  • FBal0155975
References (20)