FlyBase Allele Report: Dmel\Prosβ6[1.UAS]

General Information

Symbol

Dmel\Prosβ6^1.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0101587

Feature type

allele

Associated gene

Dmel\Prosβ6

Associated Insertion(s)

Carried in Construct

P{UAS-DTS5.S}

Also Known As

UAS-DTS5, UAS-DTS5-11

Allele class

Mutagen

in vitro construct - regulatory fusion

Nature of the Allele

Allele class

Mutagen

in vitro construct - regulatory fusion

(Schweisguth, 1999)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Prosβ6¹

(Schweisguth, 1999)

Carried in construct

P{UAS-DTS5.S}

Cytology

Nature of the lesion

Statement

Reference

Expression of a 1.2kb BamHI-NheI genomic fragment including the Pros26¹ allele is governed by UAS regulatory sequences.

(Schweisguth, 1999)

Allele components

Product class / Tool use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Prosβ6

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates autosomal dominant cerebellar ataxia

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Hsap\ATXN3^{fl.Q27.UAS.Tag:MYC}

(Warrick et al., 2005)

exacerbates Kennedy's disease

modeled by Hsap\AR^{Q112.UAS.Tag:HA}

(Chan et al., 2002)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

lethal | heat sensitive, with Scer\GAL4^sca-537.4

(Schweisguth, 1999)

Phenotype Manifest In

eo neuron, with Scer\GAL4^sca-537.4

(Schweisguth, 1999)

eye, with Scer\GAL4^GMR.PF

(Chan et al., 2002)

macrochaeta, with Scer\GAL4^sca-537.4

(Schweisguth, 1999)

ommatidial precursor cluster | heat sensitive, with Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2010)

thecogen cell | ectopic, with Scer\GAL4^sca-537.4

(Schweisguth, 1999)

tormogen cell | ectopic, with Scer\GAL4^sca-537.4

(Schweisguth, 1999)

trichogen cell, with Scer\GAL4^sca-537.4

(Schweisguth, 1999)

Detailed Description

Statement

Reference

Expression of Pros26^1.Scer\UAS using Scer\GAL4^GMR.PF at 29[o]C results in considerable disorganization of ommatidial units in larval eye discs.

(Mallik and Lakhotia, 2010)

Expression of Pros26^1.Scer\UAS under the control of Scer\GAL4^GMR.PF does not result in a rough eye phenotype.

(Shulman and Feany, 2003)

Expression of Pros26^1.Scer\UAS in the eye with Scer\GAL4^GMR.PF causes a mild rough eye phenotype ad occasional loss of pigmentation in approximately 20% of flies.

(Chan et al., 2002)

When expression is driven by two copies of Scer\GAL4^sca-537.4, temperature sensitive lethality results. Flies die as pharate adults showing a strong multiple socket phenotype. When expression is driven by one copy of Scer\GAL4^sca-537.4 at 29^oC from 0 to 24hr APF the majority of sense organs appear to be wild type. Those sense organs that are mutant show double socket or sheath cells.

(Schweisguth, 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Prosβ6^1.UAS, Scer\GAL4^GMR.PU is an enhancer of visible phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(Arya et al., 2010)

Prosβ6^1.UAS, Scer\GAL4^GMR.PU is an enhancer of visible phenotype of Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Arya et al., 2010)

NOT Enhancer of

Statement

Reference

Scer\GAL4^GMR.PU, Prosβ6^1.UAS is a non-enhancer of visible phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Hsp60D^{dsRNA.Sym.UAS}/Hsp60D^{a.Sym.Scer\UAS}, Scer\GAL4^GMR.PU

(Arya et al., 2010)

Prosβ6^1.UAS, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a non-enhancer of visible phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

NOT Suppressor of

Statement

Reference

Prosβ6^1.UAS, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a non-suppressor of visible phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

Other

Statement

Reference

Prosβ2¹, Prosβ6^1.UAS, Scer\GAL4^sca-537.4 has lethal phenotype

(Schweisguth, 1999)

Phenotype Manifest In

Enhancer of

Statement

Reference

Prosβ6^1.UAS, Scer\GAL4^GMR.PU is an enhancer of ommatidium phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(Arya et al., 2010)

Prosβ6^1.UAS, Scer\GAL4^GMR.PU is an enhancer of ommatidium phenotype of Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Arya et al., 2010)

Prosβ6^1.UAS, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsap\AR^{Q112.UAS.Tag:HA}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF

(Chan et al., 2002)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, Prosβ6^1.UAS/Hsap\HSPA1L^UAS.cWa is an enhancer of eye phenotype of Hsap\AR^{Q112.UAS.Tag:HA}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF

(Chan et al., 2002)

NOT Enhancer of

Statement

Reference

Scer\GAL4^GMR.PU, Prosβ6^1.UAS is a non-enhancer of ommatidium phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Hsp60D^{dsRNA.Sym.UAS}/Hsp60D^{a.Sym.Scer\UAS}, Scer\GAL4^GMR.PU

(Arya et al., 2010)

Prosβ6^1.UAS, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

Suppressor of

Statement

Reference

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, Prosβ6^1.UAS/Hsap\HSPA1L^UAS.cWa is a suppressor of eye phenotype of Hsap\AR^{Q112.UAS.Tag:HA}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF

(Chan et al., 2002)

NOT Suppressor of

Statement

Reference

Prosβ6^1.UAS, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Prosβ2¹ shows synthetic non-conditional lethality with Pros26^1.Scer\UAS, Scer\GAL4^sca-537.4.

(Schweisguth, 1999)

Xenogenetic Interactions

Statement

Reference

Co-expression of Pros26^1.Scer\UAS via Scer\GAL4^elav.PU does not significantly affect the Hsp60D^{dsRNA.Sym.Scer\UAS}-mediated partial suppression of the eye degeneration phenotype caused by Hsap\MJD^{tr.Q78.Scer\UAS.T:Ivir\HA1} overexpression. Co-expression of Pros26^1.Scer\UAS via Scer\GAL4^elav.PU does not significantly affect the Hsp60D^{dsRNA.Sym.Scer\UAS}-mediated partial suppression of the eye degeneration phenotype caused by Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} overexpression. The eye degeneration caused by Hsap\MJD^{tr.Q78.Scer\UAS.T:Ivir\HA1} overexpression via Scer\GAL4^GMR.PU is enhanced by co-expression of Pros26^1.Scer\UAS. The eye degeneration caused by Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} overexpression via Scer\GAL4^GMR.PU is enhanced by co-expression of Pros26^1.Scer\UAS.

(Arya et al., 2010)

Co-expression of Pros26^1.Scer\UAS suppresses the ability of Hsap\MJD^{fl.Q27.Scer\UAS.T:Hsap\MYC} to restore the eye and retina phenotypes towards normal in flies expressing Hsap\MJD^{tr.Q78.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR.PF, such that the triple mutant flies have a reduction in eye pigmentation, show collapse of the eye and loss of internal retinal tissue.

(Warrick et al., 2005)

The rough eye phenotype caused by expression of Hsap\MAPT^{V337M.Scer\UAS} under the control of Scer\GAL4^GMR.PF is not modified if the flies are also carrying Pros26^1.Scer\UAS.

(Shulman and Feany, 2003)

Co-expression of Pros26^1.Scer\UAS with Hsap\AR^{Q112.Scer\UAS.T:Ivir\HA1} (both under the control of Scer\GAL4^GMR.PF) enhances the eye degeneration found with expression of Hsap\AR^{Q112.Scer\UAS.T:Ivir\HA1} alone. Analysis of protein extracts reveals that the enhancement of degneration is associated with a change in the solubility properties of the Hsap\AR^{Q112.Scer\UAS.T:Ivir\HA1} protein. Co-expression of Pros26^1.Scer\UAS and Hsap\HSPA1L^Scer\UAS.cWa, together with the pathogenic Hsap\AR^{Q112.Scer\UAS.T:Ivir\HA1} lines (all under the control of the Scer\GAL4^GMR.PF driver) suppress the eye phenotype. Co-expression of Pros26^1.Scer\UAS and Hsc70-4^{K71S.Scer\UAS}, together with the pathogenic Hsap\AR^{Q112.Scer\UAS.T:Ivir\HA1} lines (all under the control of the Scer\GAL4^GMR.PF driver) enhances the eye phenotype.

(Chan et al., 2002)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Pros26^1.Scer\UAS

Prosβ6^1.Scer\UAS

Prosβ6^1.UAS

UAS-DTS

(Zirin et al., 2015)

UAS-DTS5

(Zirin et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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