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General Information
Symbol
Dmel\Prosβ61.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0101587
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-DTS5, UAS-DTS5-11
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
Expression of a 1.2kb BamHI-NheI genomic fragment including the Pros261 allele is governed by UAS regulatory sequences.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Expression of Pros261.Scer\UAS using Scer\GAL4GMR.PF at 29[o]C results in considerable disorganization of ommatidial units in larval eye discs.
Expression of Pros261.Scer\UAS under the control of Scer\GAL4GMR.PF does not result in a rough eye phenotype.
Expression of Pros261.Scer\UAS in the eye with Scer\GAL4GMR.PF causes a mild rough eye phenotype ad occasional loss of pigmentation in approximately 20% of flies.
When expression is driven by two copies of Scer\GAL4sca-537.4, temperature sensitive lethality results. Flies die as pharate adults showing a strong multiple socket phenotype. When expression is driven by one copy of Scer\GAL4sca-537.4 at 29oC from 0 to 24hr APF the majority of sense organs appear to be wild type. Those sense organs that are mutant show double socket or sheath cells.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Prosβ21 shows synthetic non-conditional lethality with Pros261.Scer\UAS, Scer\GAL4sca-537.4.
Xenogenetic Interactions
Statement
Reference
Co-expression of Pros261.Scer\UAS via Scer\GAL4elav.PU does not significantly affect the Hsp60DdsRNA.Sym.Scer\UAS-mediated partial suppression of the eye degeneration phenotype caused by Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 overexpression. Co-expression of Pros261.Scer\UAS via Scer\GAL4elav.PU does not significantly affect the Hsp60DdsRNA.Sym.Scer\UAS-mediated partial suppression of the eye degeneration phenotype caused by Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 overexpression. The eye degeneration caused by Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 overexpression via Scer\GAL4GMR.PU is enhanced by co-expression of Pros261.Scer\UAS. The eye degeneration caused by Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 overexpression via Scer\GAL4GMR.PU is enhanced by co-expression of Pros261.Scer\UAS.
Co-expression of Pros261.Scer\UAS suppresses the ability of Hsap\MJDfl.Q27.Scer\UAS.T:Hsap\MYC to restore the eye and retina phenotypes towards normal in flies expressing Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF, such that the triple mutant flies have a reduction in eye pigmentation, show collapse of the eye and loss of internal retinal tissue.
The rough eye phenotype caused by expression of Hsap\MAPTV337M.Scer\UAS under the control of Scer\GAL4GMR.PF is not modified if the flies are also carrying Pros261.Scer\UAS.
Co-expression of Pros261.Scer\UAS with Hsap\ARQ112.Scer\UAS.T:Ivir\HA1 (both under the control of Scer\GAL4GMR.PF) enhances the eye degeneration found with expression of Hsap\ARQ112.Scer\UAS.T:Ivir\HA1 alone. Analysis of protein extracts reveals that the enhancement of degneration is associated with a change in the solubility properties of the Hsap\ARQ112.Scer\UAS.T:Ivir\HA1 protein. Co-expression of Pros261.Scer\UAS and Hsap\HSPA1LScer\UAS.cWa, together with the pathogenic Hsap\ARQ112.Scer\UAS.T:Ivir\HA1 lines (all under the control of the Scer\GAL4GMR.PF driver) suppress the eye phenotype. Co-expression of Pros261.Scer\UAS and Hsc70-4K71S.Scer\UAS, together with the pathogenic Hsap\ARQ112.Scer\UAS.T:Ivir\HA1 lines (all under the control of the Scer\GAL4GMR.PF driver) enhances the eye phenotype.
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
Pros261.Scer\UAS
Prosβ61.Scer\UAS
Prosβ61.UAS
Name Synonyms
Secondary FlyBase IDs
    References (9)