Allele Dmel\Mef2^III.Scer\UAS

General Information
Symbol	Dmel\Mef2^III.Scer\UAS	Species	D. melanogaster
Name		FlyBase ID	FBal0101611
Feature type	allele	Created / Updated	2006-08-20/2006-08-20
Associated gene	Dmel\Mef2

Allele class
Mutagen	in vitro construct \| regulatory fusion
Nature of the Allele
Allele class
Mutagen	in vitro construct \| regulatory fusion (Gunthorpe et al., 1999)
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of the type III Mef2 isoform. (Gunthorpe et al., 1999)
Assay mode	In transgenic Drosophila (intraspecific) (Gunthorpe et al., 1999)
Carried in construct	P{UAS-Mef2.III} (Ruiz-Gomez et al., 2002, Gunthorpe et al., 1999)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In
	dorsal closure embryo, with Scer\GAL4^da.G32 (Gunthorpe et al., 1999) denticle belt, with Scer\GAL4^da.G32 (Gunthorpe et al., 1999) embryonic/first instar larval cuticle, with Scer\GAL4^da.G32 (Gunthorpe et al., 1999)
Detailed Description
	Statement Reference Dorsal closure fails in embryos expressing Mef2^III.Scer\UAS under the control of Scer\GAL4^da.G32. The cuticle is thin but has denticle belts, although their pattern is abnormal. Expression of Mef2^III.Scer\UAS under the control of Scer\GAL4^twi.PB results in embryos that are phenotypically wild type. (Gunthorpe et al., 1999)
Interactions
Phenotypic Class
Phenotype Manifest In
NOT Suppressor of
Statement Reference Scer\GAL4^twi.PB/Mef2^III.Scer\UAS is a non-suppressor of embryonic myoblast phenotype of lmd^A388 (Ruiz-Gomez et al., 2002)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Scer\GAL4^how-24B/Mef2^III.Scer\UAS rescues Mef2^22-21 (Gunthorpe et al., 1999)
Comments	At 18^oC, expression of Mef2^III.Scer\UAS under the control of Scer\GAL4^how-24B rescues midgut constrictions in 90% of Mef2^22-21 embryos. The pharyngeal muscles are wild-type in 50% of the embryos. A subset of muscles, including some dorsal (dorsal transverse muscle 1), some lateral (lateral longitudinal muscle 1, segment border muscle) and some ventral muscles (ventral transverse muscle 1, ventral acute muscle 1, ventral acute muscle 2) are rescued. Unfused myoblasts are seen in the majority of hemisegments. The heart shows a range of phenotypes in these embryos. At 25^oC, expression of Mef2^III.Scer\UAS under the control of Scer\GAL4^how-24B rescues midgut constrictions in Mef2^22-21 embryos. The shape of the pharyngeal muscle cells is not wild type in 30% of embryos, while it is fully rescued in other embryos. The somatic musculature is almost rescued to wild type, although occasionally one or two muscles in a hemisegment are absent or have an abnormal shape. The variety of phenotypes seen in the heart is similar to that seen when Mef2^III.Scer\UAS is expressed under the control of Scer\GAL4^how-24B at 18^oC. At 29^oC, when Mef2^III.Scer\UAS is expressed under the control of Scer\GAL4^how-24B in Mef2^22-21 embryos there are no midgut constrictions. The pharyngeal muscles are completely rescued. The heart is disorganised posteriorly in some embryos. The somatic musculature is fully rescued; the pattern of myosin-expressing fibres is essentially wild type, although there are some unfused myoblasts in the majority of hemisegments. (Gunthorpe et al., 1999)
Stocks ( 0 )

Notes on Origin
Discoverer

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Mef2^III.Scer\UAS
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Ruiz-Gomez et al., 2002, Development 129(1): 133--141 myoblasts incompetent encodes a zinc finger transcription factor required to specify fusion-competent myoblasts in Drosophila. [FBrf0141504] Gunthorpe et al., 1999, Dev. Biol. 215(1): 130--145 Different levels, but not different isoforms, of the Drosophila transcription factor DMEF2 affect distinct aspects of muscle differentiation. [FBrf0111895]

Allele Dmel\Mef2III.Scer\UAS

Allele Dmel\Mef2^III.Scer\UAS