Allele Dmel\mys^Scer\UAS.cDa

General Information
Symbol	Dmel\mys^Scer\UAS.cDa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Diamond	FlyBase ID	FBal0102506
Feature type	allele	Associated gene	Dmel\mys

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Beumer et al., 1999)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive mys expression. (Beumer et al., 1999)
Carried in construct	P{UAS-mys.D} (Beumer et al., 2002, Beumer et al., 1999, Buff et al., 2007, Han et al., 2012, Issigonis et al., 2009)
Cytology
Phenotypic Data
Phenotypic Class
	neuroanatomy defective \| larval stage, with Scer\GAL4^F11 (Beumer et al., 1999) neuroanatomy defective \| third instar larval stage, with Scer\GAL4^ppk.PH (Han et al., 2012)
Phenotype Manifest In
	bouton & neuromuscular junction, with Scer\GAL4^elav-C155 (Beumer et al., 1999) bouton & neuromuscular junction, with Scer\GAL4^F11 (Beumer et al., 1999) bouton & neuromuscular junction, with Scer\GAL4^α (Beumer et al., 1999) bouton & neuromuscular junction & abdominal ventral longitudinal muscle 1, with Scer\GAL4^F11 (Beumer et al., 1999) bouton & neuromuscular junction & abdominal ventral longitudinal muscle 1, with Scer\GAL4^α (Beumer et al., 1999) bouton & neuromuscular junction & abdominal ventral longitudinal muscle 3, with Scer\GAL4^F11 (Beumer et al., 1999) bouton & neuromuscular junction & abdominal ventral longitudinal muscle 3, with Scer\GAL4^α (Beumer et al., 1999) dorsal multidendritic neuron ddaC \| third instar larval stage, with Scer\GAL4^ppk.PH (Han et al., 2012) male germline stem cell, with Scer\GAL4^C587 (Issigonis et al., 2009) neuromuscular junction, with Scer\GAL4^elav-C155 (Beumer et al., 1999) neuromuscular junction, with Scer\GAL4^F11 (Beumer et al., 1999) neuromuscular junction, with Scer\GAL4^α (Beumer et al., 1999) neuromuscular junction & abdominal lateral longitudinal muscle 1, with Scer\GAL4^F11 (Beumer et al., 1999) neuromuscular junction & abdominal lateral longitudinal muscle 1, with Scer\GAL4^α (Beumer et al., 1999) neuromuscular junction & abdominal ventral longitudinal muscle 1, with Scer\GAL4^F11 (Beumer et al., 1999) neuromuscular junction & abdominal ventral longitudinal muscle 1, with Scer\GAL4^α (Beumer et al., 1999) neuromuscular junction & abdominal ventral longitudinal muscle 3, with Scer\GAL4^F11 (Beumer et al., 1999) neuromuscular junction & abdominal ventral longitudinal muscle 3, with Scer\GAL4^α (Beumer et al., 1999)
Detailed Description
	Statement Reference Expression of mys[Scer\UAS.cDa] in class IV da neurons under the control of Scer\GAL4[ppk.PH] causes significant dendritic reduction. (Han et al., 2012) Overexpression of mys[Scer\UAS.cDa] driven by Scer\GAL4[C587] in cyst cells and cyst progenitor cells reduces the number of germline stem cells per testis significantly. (Issigonis et al., 2009) Expression of mys^Scer\UAS.cDa under the control of Scer\GAL4^elav-C155 results in a significant reduction in branching and bouton numbers in the neuromuscular junctions of larvae. However, bouton number is normal and there is no increase in the number of mini-boutons. Expression of mys^Scer\UAS.cDa under the control of Scer\GAL4^α or Scer\GAL4^F11 results in a significant reduction in branching and bouton numbers in the neuromuscular junctions of muscles 6 and 12, and a significant reduction in branching but not bouton numbers in the neuromuscular junctions of muscle 4. A moderate increase in the number of mini-boutons is seen. (Beumer et al., 1999)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Scer\GAL4^ppk.PH, mys^Scer\UAS.cDa has neuroanatomy defective \| third instar larval stage phenotype, suppressible \| partially by mew^{Scer\UAS.cMBa}/Scer\GAL4^ppk.PH (Han et al., 2012)
Enhancer of
Statement Reference mys^Scer\UAS.cDa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of visible phenotype of Ppt1^Scer\UAS.cKa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Buff et al., 2007)
Suppressor of
Statement Reference mew^{Scer\UAS.cMBa}, mys^Scer\UAS.cDa, Scer\GAL4^ppk.PH is a suppressor of neuroanatomy defective \| third instar larval stage phenotype of fry⁰²²⁴⁰/fry¹ (Han et al., 2012) mew^{Scer\UAS.cMBa}, mys^Scer\UAS.cDa, Scer\GAL4^ppk.PH is a suppressor of neuroanatomy defective \| third instar larval stage phenotype of Sin1^e03756 (Han et al., 2012)
Other
Statement Reference Scer\GAL4^ppk.PH, mew^{Scer\UAS.cMBa}, mys^Scer\UAS.cDa has neuroanatomy defective \| third instar larval stage phenotype (Han et al., 2012)
Phenotype Manifest In
Suppressed by
Statement Reference Scer\GAL4^ppk.PH, mys^Scer\UAS.cDa has dorsal multidendritic neuron ddaC \| third instar larval stage phenotype, suppressible \| partially by mew^{Scer\UAS.cMBa}/Scer\GAL4^ppk.PH (Han et al., 2012)
Enhancer of
Statement Reference mys^Scer\UAS.cDa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Ppt1^Scer\UAS.cKa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Buff et al., 2007)
Suppressor of
Statement Reference mew^{Scer\UAS.cMBa}, mys^Scer\UAS.cDa, Scer\GAL4^ppk.PH is a suppressor of dorsal multidendritic neuron ddaC \| third instar larval stage phenotype of fry⁰²²⁴⁰/fry¹ (Han et al., 2012) mew^{Scer\UAS.cMBa}, mys^Scer\UAS.cDa, Scer\GAL4^ppk.PH is a suppressor of dorsal multidendritic neuron ddaC \| third instar larval stage phenotype of Sin1^e03756 (Han et al., 2012)
Other
Statement Reference Scer\GAL4^ppk.PH, mew^{Scer\UAS.cMBa}, mys^Scer\UAS.cDa has ventral multidendritic neuron vdaB \| third instar larval stage phenotype (Han et al., 2012)
Additional Comments
Genetic Interactions
Statement Reference Expression of mew[Scer\UAS.cMBa] largely rescues the dendritic reduction seen when mys[Scer\UAS.cDa] is expressed in class IV da neurons under the control of Scer\GAL4[ppk.PH]. Co-expression of mys[Scer\UAS.cDa] and mew[Scer\UAS.cMBa] under the control of Scer\GAL4[ppk.PH] completely eliminates the enclosure of ventral midline vdaB neurons in the epidermis. 8.43% of vdaB neurons are enclosed in wild type. All associated non-contacting dendritic crossing is also suppressed. Co-expression of mys[Scer\UAS.cDa] and mew[Scer\UAS.cMBa] the control of Scer\GAL4[ppk.PH] rescues the increase in epidermally enclosed dendrites seen in fry[1]/fry[02240] mutants. The dendritic crossing seen in fry[1]/fry[02240] mutants is also largely rescued. The dendritic crossings that remain in the rescue flies are between two ECM attached dendrites, rather than non-contacting crossings between an epidermally enclosed dendrite and one attached to the ECM. The crossing on the v'ada/vdaB interface is also mostly rescued and all non-contacting crossing is eliminated. (Han et al., 2012)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	mys^Scer\UAS.cDa/Scer\GAL4^Mhc.PW rescues mys^b9 (Beumer et al., 2002)
Fails to rescue	mys^Scer\UAS.cDa/Scer\GAL4^Mhc.PW fails to rescue mys^ts1 (Beumer et al., 2002)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer	M. Diamond

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	mys^Scer\UAS.cDa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Diamond (Beumer et al., 1999)
Secondary FlyBase IDs

References ( 5 )
Research paper	Han et al., 2012, Neuron 73(1): 64--78 Integrins Regulate Repulsion-Mediated Dendritic Patterning of Drosophila Sensory Neurons by Restricting Dendrites in a 2D Space. [FBrf0217239] Issigonis et al., 2009, Science 326(5949): 153--156 JAK-STAT signal inhibition regulates competition in the Drosophila testis stem cell niche. [FBrf0209165] Buff et al., 2007, Genetics 176(1): 209--220 Genetic modifiers of Drosophila palmitoyl-protein thioesterase 1-induced degeneration. [FBrf0201672] Beumer et al., 2002, Development 129(14): 3381--3391 Integrins regulate DLG/FAS2 via a CaM kinase II-dependent pathway to mediate synapse elaboration and stabilization during postembryonic development. [FBrf0151260] Beumer et al., 1999, Development 126(24): 5833--5846 A role for PS integrins in morphological growth and synaptic function at the postembryonic neuromuscular junction of Drosophila. [FBrf0111801]

Allele Dmel\mysScer\UAS.cDa

Allele Dmel\mys^Scer\UAS.cDa