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General Information
Symbol
Dmel\mysUAS.cDa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Diamond
FlyBase ID
FBal0102506
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-mys
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive mys expression.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

bouton & neuromuscular junction, with Scer\GAL4elav-C155

bouton & neuromuscular junction, with Scer\GAL4F11

bouton & neuromuscular junction, with Scer\GAL4α

bouton & neuromuscular junction & abdominal ventral longitudinal muscle 1, with Scer\GAL4F11

bouton & neuromuscular junction & abdominal ventral longitudinal muscle 1, with Scer\GAL4α

bouton & neuromuscular junction & abdominal ventral longitudinal muscle 3, with Scer\GAL4F11

bouton & neuromuscular junction & abdominal ventral longitudinal muscle 3, with Scer\GAL4α

neuromuscular junction & abdominal lateral longitudinal muscle 1, with Scer\GAL4F11

neuromuscular junction & abdominal lateral longitudinal muscle 1, with Scer\GAL4α

neuromuscular junction & abdominal ventral longitudinal muscle 1, with Scer\GAL4F11

neuromuscular junction & abdominal ventral longitudinal muscle 1, with Scer\GAL4α

neuromuscular junction & abdominal ventral longitudinal muscle 3, with Scer\GAL4F11

neuromuscular junction & abdominal ventral longitudinal muscle 3, with Scer\GAL4α

Detailed Description
Statement
Reference

Expression of mysScer\UAS.cDa in class IV da neurons under the control of Scer\GAL4ppk.PH causes significant dendritic reduction.

Overexpression of mysScer\UAS.cDa driven by Scer\GAL4C587 in cyst cells and cyst progenitor cells reduces the number of germline stem cells per testis significantly.

Expression of mysScer\UAS.cDa under the control of Scer\GAL4elav-C155 results in a significant reduction in branching and bouton numbers in the neuromuscular junctions of larvae. However, bouton number is normal and there is no increase in the number of mini-boutons. Expression of mysScer\UAS.cDa under the control of Scer\GAL4α or Scer\GAL4F11 results in a significant reduction in branching and bouton numbers in the neuromuscular junctions of muscles 6 and 12, and a significant reduction in branching but not bouton numbers in the neuromuscular junctions of muscle 4. A moderate increase in the number of mini-boutons is seen.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Enhancer of
Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

Simultaneous expression of mewScer\UAS.cMBa and mysScer\UAS.cDa under the control of Scer\GAL4ppk.PU in the Sema-2bf02042/Df(2R)Sema-2b-C4 mutant larvae background fully rescued the increased dendritic crossing phenotype in the class IV da neurons displayed by the Sema-2bf02042/Df(2R)Sema-2b-C4 mutants.

Co-expression of mysScer\UAS.cDa and mewScer\UAS.cMBa under the control of Scer\GAL4elav-C155 suppresses the number of dendrite-dendrite crossings seen in rawjj102 mutant class IV dendritic arborizing neuron clones. Terminal branch number is significantly restored and a greater fraction of stable terminals are seen compared. However, the mutant terminal dendrite elongation defect is not rescued.

Co-expression of mysScer\UAS.cDa and mewScer\UAS.cMBa in class IV dendritic arborizing neurons under the control of Scer\GAL4ppk.PH partially suppresses the dendrite growth seen in RetC168/Df(2L)BSC312 mutant larvae and completely suppresses the loss of dendrite-ECM interaction. The reduction in dendritic field coverage is not suppressed.

Expression of mewScer\UAS.cMBa largely rescues the dendritic reduction seen when mysScer\UAS.cDa is expressed in class IV da neurons under the control of Scer\GAL4ppk.PH.

Co-expression of mysScer\UAS.cDa and mewScer\UAS.cMBa under the control of Scer\GAL4ppk.PH completely eliminates the enclosure of ventral midline vdaB neurons in the epidermis. 8.43% of vdaB neurons are enclosed in wild type. All associated non-contacting dendritic crossing is also suppressed.

Co-expression of mysScer\UAS.cDa and mewScer\UAS.cMBa the control of Scer\GAL4ppk.PH rescues the increase in epidermally enclosed dendrites seen in fry1/fry02240 mutants. The dendritic crossing seen in fry1/fry02240 mutants is also largely rescued. The dendritic crossings that remain in the rescue flies are between two ECM attached dendrites, rather than non-contacting crossings between an epidermally enclosed dendrite and one attached to the ECM. The crossing on the v'ada/vdaB interface is also mostly rescued and all non-contacting crossing is eliminated.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
mysScer\UAS.cDa
mysUAS.cDa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Diamond
Secondary FlyBase IDs
    References (9)