Allele Dmel\mam^H.Scer\UAS

General Information
Symbol	Dmel\mam^H.Scer\UAS	Species	D. melanogaster
Name		FlyBase ID	FBal0102514
Feature type	allele	Associated gene	Dmel\mam

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - deletion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - deletion (Helms et al., 1999) in vitro construct - regulatory fusion (Helms et al., 1999)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: A truncated mam open reading frame, ending at nucleotide 1489 (amino acid residue 245), is driven under the control of Scer\UAS sequences. Sequences located carboxy to the basic cluster are removed, including both acidic charge clusters. (Helms et al., 1999)
Carried in construct	P{UAS-mamH} (Yedvobnick et al., 2001, Dutta et al., 2004, Yedvobnick et al., 2004, Helms et al., 1999, Alexander et al., 2006, Song et al., 2007, Parks and Muskavitch, 2009.2.3)
Cytology
Phenotypic Data
Phenotypic Class
	neuroanatomy defective \| heat sensitive, with Scer\GAL4^hs.PH (Yedvobnick et al., 2004) visible, with Scer\GAL4[-]: (Helms et al., 1999) visible, with Scer\GAL4³⁰⁹ (Helms et al., 1999) visible, with Scer\GAL4^bbg-C96 (Yedvobnick et al., 2001) visible \| adult stage, with Scer\GAL4³⁰⁹ (Alexander et al., 2006) visible \| adult stage, with Scer\GAL4³⁰⁹, mam^EP2022.A (Alexander et al., 2006) visible \| adult stage, with Scer\GAL4^bbg-C96 (Alexander et al., 2006) visible \| adult stage, with Scer\GAL4^bbg-C96, mam^EP2022.A (Alexander et al., 2006) visible \| heat sensitive, with Scer\GAL4^hs.PH (Yedvobnick et al., 2004)
Phenotype Manifest In
	eye \| heat sensitive, with Scer\GAL4^hs.PH (Yedvobnick et al., 2004) female germline stem cell, with Scer\GAL4^C587 (Song et al., 2007) germarium cap cell, with Scer\GAL4^C587 (Song et al., 2007) macrochaeta, with Scer\GAL4³⁰⁹ (Helms et al., 1999) macrochaeta \| ectopic, with Scer\GAL4³⁰⁹ (Helms et al., 1999) macrochaeta \| supernumerary \| heat sensitive, with Scer\GAL4^hs.PH (Yedvobnick et al., 2004) notopleural bristle, with Scer\GAL4³⁰⁹ (Helms et al., 1999) posterior notopleural bristle, with Scer\GAL4³⁰⁹ (Alexander et al., 2006) posterior notopleural bristle, with Scer\GAL4³⁰⁹, mam^EP2022.A (Alexander et al., 2006) posterior notopleural bristle \| ectopic, with Scer\GAL4³⁰⁹ (Helms et al., 1999) RP2 motor neuron \| ectopic \| heat sensitive, with Scer\GAL4^hs.PH (Yedvobnick et al., 2004) RP2sib neuron \| heat sensitive, with Scer\GAL4^hs.PH (Yedvobnick et al., 2004) scutellar bristle, with Scer\GAL4[-]: (Helms et al., 1999) scutellar bristle, with Scer\GAL4³⁰⁹ (Helms et al., 1999) scutellar bristle \| ectopic, with Scer\GAL4³⁰⁹ (Helms et al., 1999) wing, with Scer\GAL4^bbg-C96 (Yedvobnick et al., 2001, Helms et al., 1999) wing \| heat sensitive, with Scer\GAL4^hs.PH (Yedvobnick et al., 2004) wing margin, with Scer\GAL4^bbg-C96 (Alexander et al., 2006) wing margin, with Scer\GAL4^bbg-C96, mam^EP2022.A (Alexander et al., 2006) wing margin bristle, with Scer\GAL4^bbg-C96 (Helms et al., 1999)
Detailed Description
	Statement Reference The number of cap cells and germline stem cells in the ovaries of 2 day old females expressing mam^H.Scer\UAS under the control of Scer\GAL4^C587 is significantly reduced compared to wild type. (Song et al., 2007) Expression of mam^H.Scer\UAS under the control of Scer\GAL4^C96 produces nicks around the wing margin with 100% penetrance (whereas mam^H.Scer\UAS heterozygotes do not exhibit any wing phenotypes). This phenotype is partially suppressed in flies also heterozygous for mam^EP2022.A. Expression of mam^H.Scer\UAS under the control of Scer\GAL4³⁰⁹ elicits an increase in the number of bristles at the site of the posterior notopleural macrochaete (whereas mam^H.Scer\UAS heterozygotes do not exhibit any bristle phenotypes at this site). This phenotype is partially suppressed in flies also heterozygous for mam^EP2022.A. (Alexander et al., 2006) mam^H.Scer\UAS; Scer\GAL4¹¹⁵¹ pupae have normal numbers of dorso-ventral indirect flight muscle founder cells. (Dutta et al., 2004) Expression of mam^H.Scer\UAS under the control of Scer\GAL4^hs.PH between 2 and 6 hours of development results in a neurogenic phenotype. Expression of mam^H.Scer\UAS under the control of Scer\GAL4^hs.PH between 6 and 8 hours of development results in the GMC-1 dividing symmetrically into two RP2 neurons in about 10% of hemisegments. Expression of mam^H.Scer\UAS under the control of Scer\GAL4^hs.PH during the third larval instar results in duplications of the macrochaetae, scarring of the eye and severe loss of the wing blade. (Yedvobnick et al., 2004) Shows a low level of scutellar bristle duplications even without a Scer\GAL4 driver. When driven by Scer\GAL4³⁰⁹ perturbations are observed in bristle number predominantly at scutellar and notopleural sites, duplications of macrochaetae are seen at most scutellar sites and posterior notopleural sites. The macrochaetae multiplication phenotype is enhanced by the addition of mam¹. When mam^H.Scer\UAS is driven by Scer\GAL4^C96 wing nicks are seen and there is a loss of wing margin bristles. The addition of mam¹ produces a stronger wing nicking phenotype that is incompletely penetrant. (Helms et al., 1999)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Scer\GAL4³⁰⁹, mam^H.Scer\UAS has visible \| adult stage phenotype, enhanceable by Dl^EP3816.A, Scer\GAL4³⁰⁹ (Alexander et al., 2006) Scer\GAL4^bbg-C96, mam^H.Scer\UAS has visible \| adult stage phenotype, enhanceable by Dl^EP3816.A, Scer\GAL4^bbg-C96 (Alexander et al., 2006) Scer\GAL4^bbg-C96, mam^H.Scer\UAS has visible \| adult stage phenotype, enhanceable by RpL13A^EP3614.A, Scer\GAL4^bbg-C96 (Alexander et al., 2006)
Suppressed by
Statement Reference Scer\GAL4³⁰⁹, mam^H.Scer\UAS has visible \| adult stage phenotype, suppressible by RpL13A^EP3614.A, Scer\GAL4³⁰⁹ (Alexander et al., 2006)
Other
Statement Reference Df(1)N-54l9, Scer\GAL4³⁰⁹, mam^H.Scer\UAS has lethal phenotype (Helms et al., 1999)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4³⁰⁹, mam^H.Scer\UAS has posterior notopleural bristle phenotype, enhanceable by Dl^EP3816.A, Scer\GAL4³⁰⁹ (Alexander et al., 2006) Scer\GAL4^bbg-C96, mam^H.Scer\UAS has wing margin phenotype, enhanceable by Dl^EP3816.A, Scer\GAL4^bbg-C96 (Alexander et al., 2006) Scer\GAL4^bbg-C96, mam^H.Scer\UAS has wing margin phenotype, enhanceable by RpL13A^EP3614.A, Scer\GAL4^bbg-C96 (Alexander et al., 2006)
Suppressed by
Statement Reference Scer\GAL4³⁰⁹, mam^H.Scer\UAS has posterior notopleural bristle phenotype, suppressible by RpL13A^EP3614.A, Scer\GAL4³⁰⁹ (Alexander et al., 2006)
Additional Comments
Genetic Interactions
Statement Reference The wing margin nicking phenotype produced by expression of mam^H.Scer\UAS under the control of Scer\GAL4^C96 is enhanced in flies also heterozygous for RpL13A^EP3614.A. The wing margin nicking phenotype produced by expression of mam^H.Scer\UAS under the control of Scer\GAL4^C96 is slightly enhanced in flies also heterozygous for Dl^EP3816.A, though the phenotypic effects are largely additive. The increase in bristle number at the site of the posterior notopleural macrochaete produced by expression of mam^H.Scer\UAS under the control of Scer\GAL4³⁰⁹ is enhanced in flies also heterozygous for Dl^EP3816.A. The increase in bristle number at the site of the posterior notopleural macrochaete produced by expression of mam^H.Scer\UAS under the control of Scer\GAL4³⁰⁹ is slightly suppressed in flies also heterozygous for RpL13A^EP3614.A as fewer bristles are evident in some cases. (Alexander et al., 2006) The addition of Df(1)N-54l9 to flies expressing mam^H.Scer\UAS under the control of Scer\GAL4³⁰⁹ produces lethality. (Helms et al., 1999)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	mam^H.Scer\UAS
Name Synonym
Secondary FlyBase IDs

References ( 7 )
Research paper	Song et al., 2007, Development 134(6): 1071--1080 Notch signaling controls germline stem cell niche formation in the Drosophila ovary. [FBrf0201538] Alexander et al., 2006, Gene 368: 46--52 Insertional inactivation of the L13a ribosomal protein gene of Drosophila melanogaster identifies a new Minute locus. [FBrf0190498] Dutta et al., 2004, Development 131(15): 3761--3772 Founder myoblasts and fibre number during adult myogenesis in Drosophila. [FBrf0179200] Yedvobnick et al., 2004, Genetics 166(3): 1281--1289 Differential effects of Drosophila mastermind on asymmetric cell fate specification and neuroblast formation. [FBrf0174686] Yedvobnick et al., 2001, genesis 30(4): 250--258 Identification of chromosomal deficiencies that modify Drosophila mastermind mutant phenotypes. [FBrf0138541] Helms et al., 1999, Dev. Biol. 215(2): 358--374 Engineered truncations in the Drosophila mastermind protein disrupt Notch pathway function. [FBrf0111904]
Personal communication to FlyBase	Parks and Muskavitch, 2009.2.3, P{UAS-mamH}2 & P{UAS-mamN}3. P{UAS-mamH}2 & P{UAS-mamN}3. [FBrf0206638]

Allele Dmel\mamH.Scer\UAS

Allele Dmel\mam^H.Scer\UAS