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General Information
Symbol
Dmel\lola4.7.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0102518
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UASG-lola 4.7
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UAS sequences drive the expression of the lola 4.7 splice variant, subcloned as a 3.9kb EcoR1 fragment from lola cDNA 4.8.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
axon & adult antennal lobe projection neuron | somatic clone, with Scer\GAL4GH146, lolae76
axon & adult antennal lobe projection neuron | somatic clone | heat sensitive, with Scer\GAL4GH146
dendrite & adult antennal lobe projection neuron | somatic clone, with Scer\GAL4GH146, lolae76
dendrite & adult antennal lobe projection neuron | somatic clone | heat sensitive, with Scer\GAL4GH146
Detailed Description
Statement
Reference
The expression of lola4.7.Scer\UAS results in third instar larval class I abdominal dorsal multidendritic ddaD and ddaE neurons exhibiting a reduced dendritic tree (expression under the control of Scer\GAL4221) and class IV abdominal dorsal multidendritic ddaC neurons exhibiting a significant decrease in the number of dendrite branch terminals (expression under the control of Scer\GAL4ppk.PG), as compared to controls.
MARCM-mediated expression of lola4.7.Scer\UAS, using Scer\GAL4GH146, results in strong targeting defects in antero-dorsal projection neuron (adPN) and lateral (lPN) neuroblast clones. Phenotypes often include a large increase in wandering dendrites in the antennal lobe (AL) and some degree of loss of targeting and ectopic targeting. Often dendrites appear to be largely restricted to one region of the AL, such as dorsal or lateral, and have wandering dendrites through this region in many glomeruli, but fail to innervate normal targets or extend any dendrites into other regions of the AL. vPN neuroblast MARCM clones expressing lola4.7.Scer\UAS, using Scer\GAL4GH146, show a loss of targeting to DA1 and the frequent extension of dendrites outside of the AL boundary, most often in the ventral region projecting towards the suboesophageal ganglion. Processes of the pan-AL vPN are not elaborated properly and only partially innervate the AL. MARCM-mediated expression of lola4.7.Scer\UAS, using Scer\GAL4GH146, results in variable DL1 phenotypes, ranging from a complete loss of DL1 innervation and targeting to other regions of the AL, to DL1 targeting with dendritic extensions outside of the DL1 glomerular region. Axon targeting is also affected, most often resulting in a high degree of ectopic branching, axon bifurcation and incorrect lateral horn innervation patterns. MARCM-mediated expression of lola4.7.Scer\UAS, using Scer\GAL4GH146, results in few dendritic phenotypes at 18[o]C, while at 25[o]C and 29[o]C dendrites mistarget and have ectopic innervations and axons have additional branches and bifurcations. MARCM clones simultaneously mutant for lolae76 and lola4.7.Scer\UAS, induced using Scer\GAL4GH146, exhibit: i) cell loss and lack of dendritic extensions in adPN and lPN neuroblast clones; ii) a strong reduction of VA1lm and DA1 targeting in vPN clones, with the pan-AL neuron often showing little dendritic process elaboration and only rare innervation of much of the AL. DL1 MARCM clones simultaneously mutant for lolae76 and lola4.7.Scer\UAS, induced using Scer\GAL4GH146 either fail to innervate DL1 or have extensions into other regions of the AL. Axonal defects range from a failure to extend or elaborate branches in the LH to an increase in ectopic branching.
Over-expression of lola4.7.Scer\UAS by Scer\GAL4how-24B, causes lethality and striking defects in SNb development. Muscles show an apparent "overgrowth" of Fas2 staining material where SNb axons contact the ventral longitudinal muscles (VLMs). Some of this material is in the clefts between adjacent muscles, but more spread out of the clefts and over the surfaces of the muscles. In many cases, broad branches extend over the muscles quite separate from the normal projections in the clefts. There is also a striking expansion of the shaft of the SNb nerve, particularly on muscles 6 and 7. Dense punctate syt staining on the expanded neural material suggests expanded domains of synaptogenesis. These sorts of phenotype are seen in about 45% of Scer\GAL4how-24B expressing embryos bearing one copy of lola4.7.Scer\UAS, and in about 66% of embryos bearing two. Similar phenotypes are seen if lola4.7.Scer\UAS is driven by Scer\GAL4twi.PG. When two copies of lola4.7.Scer\UAS are used, very rare cases are observed where SNb seems to stall in the ventral part of the target field in association with the 6/7 cleft, but where no axons continue more dorsally to muscles 13 or 12. Over-expression of lola4.7.Scer\UAS in neurons driven by Scer\GAL4elav.PLu, causes a phenotype very similar to lola mutants. SNb axons fail to innervate the clefts between adjacent VLMs and often stall between the point of separation from the ISN and the muscle 6/13 junction. This phenotype is dose dependant, occurring at a higher frequency when multiple copies of lola4.7.Scer\UAS are present. At the highest levels of expression, A "bypass" phenotype of SNb axons failing to separate from the ISN altogether is seen. No evidence for defects in muscle morphogenesis is seen.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Fails to rescue
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
lola4.7.Scer\UAS
lola4.7.UAS
Name Synonyms
Secondary FlyBase IDs
    References (4)