FlyBase Allele Report: Dmel\lola[3.8.UAS]

General Information

Symbol

Dmel\lola^3.8.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0102519

Feature type

allele

Associated gene

Dmel\lola

Associated Insertion(s)

Carried in Construct

P{UAS-lola.3.8}

Key Links

Transgenic product class

cDNA

(Madden et al., 1999)

wild_type

(Madden et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Madden et al., 1999)

wild_type

(Madden et al., 1999)

Mutagen

in vitro construct

(Madden et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-lola.3.8}

Cytology

Description

UAS sequences drive the expression of the lola 3.8 splice variant, subcloned as a 2kb EcoR1 fragment from lola cDNA 8.13.

(Madden et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

lola

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | somatic clone, with Scer\GAL4^GH146, lola^e76

(Spletter et al., 2007)

lethal, with Scer\GAL4^GH146

(Spletter et al., 2007)

wild-type, with Scer\GAL4^elav.PLu

(Madden et al., 1999)

wild-type, with Scer\GAL4^how-24B

(Madden et al., 1999)

wild-type, with Scer\GAL4^twi.PG

(Madden et al., 1999)

Phenotype Manifest In

adult antennal lobe projection neuron | somatic clone, with Scer\GAL4^GH146, lola^e76

(Spletter et al., 2007)

adult antennal lobe projection neuron adPN | somatic clone, with Scer\GAL4^GH146, lola^e76

(Spletter et al., 2007)

adult antennal lobe projection neuron DA1 vPN | somatic clone, with Scer\GAL4^GH146, lola^e76

(Spletter et al., 2007)

adult antennal lobe projection neuron DL1 adPN | somatic clone, with Scer\GAL4^GH146, lola^e76

(Spletter et al., 2007)

adult antennal lobe projection neuron lPN | somatic clone, with Scer\GAL4^GH146, lola^e76

(Spletter et al., 2007)

adult antennal lobe projection neuron VA1v vPN | somatic clone, with Scer\GAL4^GH146, lola^e76

(Spletter et al., 2007)

adult lateral horn | somatic clone, with Scer\GAL4^GH146, lola^e76

(Spletter et al., 2007)

dendrite & adult antennal lobe projection neuron | somatic clone, with Scer\GAL4^GH146, lola^e76

(Spletter et al., 2007)

Detailed Description

Statement

Reference

MARCM-mediated expression of lola^3.8.Scer\UAS, using Scer\GAL4^GH146, in neuroblasts or single cell clones does not result in detectable defects.

MARCM clones simultaneously mutant for lola^e76 and lola^3.8.Scer\UAS, induced using Scer\GAL4^GH146, exhibit: i) cell loss and lack of dendritic extensions in adPN and lPN neuroblast clones; ii) a strong reduction of VA1lm and DA1 targeting in vPN clones, with the pan-AL neuron often showing little dendritic process elaboration and only rare innervation of much of the AL.

DL1 MARCM clones simultaneously mutant for lola^e76 and lola^3.8.Scer\UAS, induced using Scer\GAL4^GH146 are variable, with half appearing normal . Axonal defects range from a failure to extend or elaborate branches in the LH to an increase in ectopic branching.

(Spletter et al., 2007)

Overexpression of the lola 3.8 isoform, in lola^3.8.Scer\UAS produces no detectable phenotype.

(Madden et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to rescue

lola^3.8.UAS/Scer\GAL4^GH146 fails to rescue lola^e76

(Spletter et al., 2007)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

28830

P{UAS-lola.3.8}1, w^*

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

lola^3.8.Scer\UAS

lola^3.8.UAS

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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