FB2025_01 , released February 20, 2025
Allele: Scer\GAL4sev.EP
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General Information
Symbol
Scer\GAL4sev.EP
Species
S. cerevisiae
Name
FlyBase ID
FBal0102834
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{sevEP-GAL4.B}
Also Known As
sev-GAL4, sep-Gal4, sevEP-GAL4, sepGal4, sevEPGal4
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Bailey, 1999.12.9)
Progenitor genotype
Carried in construct
P{sevEP-GAL4.B}
Cytology
Description

A single copy of the sev enhancer, defined as a 1.2 kb XhoI fragment, upstream of a 1.05 kb EcoRI/EcoRV fragment containing the endogenous sev promoter guides expression of regulates expression of a GAL4 driver. A polyA signal is provided by a 170 bp BamHI fragment.

(Bailey, 1999.12.9)
Allele components
Component
Use(s)
Regulatory region(s)
sev
Encoded product / tool
GAL4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      No Assay Recorded
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      third instar larval stage
      eye disc
      (Bailey, 1999.12.9)
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      third instar larval stage
      eye disc posterior to the morphogenetic furrow
      (St Pierre et al., 2002)
      photoreceptor precursor cell | subset
      (St Pierre et al., 2002)
      mystery cell
      (St Pierre et al., 2002)
      cone cell
      (St Pierre et al., 2002)
      Additional Information
      Statement
      Reference

      Drives expression in developing photoreceptor cells in the eye disc.

      (FlyBase Curators, 2017)

      ScerGAL4sev.EP drives expression in the eye disc posterior to the morphogenetic furrow, in a subset of photoreceptor precursor cells, and in cells adjacent to the developing photoreceptor cells, likely mystery cells and cone cells.

      (St Pierre et al., 2002)

      Very little non-specific expression (sev enhancer independent).

      (Bailey, 1999.12.9)
       
      Marker for
      Reflects expression of
      sev
      (St Pierre et al., 2002, Bailey, 1999.12.9)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class

      abnormal cell polarity, with KrnUAS.cUa

      (Brown and Freeman, 2003)

      abnormal neuroanatomy, with Liprin-βRNAi.UAS

      (Astigarraga et al., 2010)

      abnormal neuroanatomy, with trioGD9531

      (Astigarraga et al., 2010)

      abnormal planar polarity, with aPKCΔN.UAS

      (Djiane et al., 2005)

      abnormal planar polarity | adult stage, with FrlDN.UAS

      (Dollar et al., 2016)

      abnormal planar polarity | third instar larval stage, with FrlDN.UAS

      (Dollar et al., 2016)

      abnormal size, with hidUAS.cIa

      (Dwivedi et al., 2015)

      decreased cell number | adult stage, with Cdc42UAS.cWa

      (Dollar et al., 2016)

      decreased cell number | adult stage, with FrlCA.UAS

      (Dollar et al., 2016)

      decreased cell number | adult stage, with FrlDN.UAS

      (Dollar et al., 2016)

      decreased cell number | third instar larval stage, with FrlDN.UAS

      (Dollar et al., 2016)

      lethal, with E2f1UAS.cNa

      (Morris et al., 2006)

      lethal, with rprUAS.cZa

      (Morris et al., 2006)

      lethal - all die during pupal stage, with Tak1UAS.cMa

      (Ray and Lakhotia, 2019)

      majority die during pharate adult stage, with Ras85DV12.UAS

      (Ray and Lakhotia, 2019)

      partially lethal - majority die, with CycEUAS.cLa

      (Morris et al., 2006)

      partially lethal - majority die, with Ras85DV12.UAS

      (Ray and Lakhotia, 2019)

      viable, with RafRBD+CRD.UAS.Tag:FLAG

      (Ray and Lakhotia, 2019)

      viable, with Tak1UAS.cMa

      (Ray and Lakhotia, 2019)

      visible, with BacA\p35UAS.cHa

      (Morris et al., 2006)

      visible, with cncUAS.cSa

      (Rahman et al., 2013, Sykiotis and Bohmann, 2008)

      visible, with CycEUAS.cLa

      (Morris et al., 2006)

      visible, with grimUAS.cUa

      (Dwivedi et al., 2015)

      visible, with panUAS.cWa

      (Jackson et al., 2002)

      visible, with Rac1N17.UAS

      (Morris et al., 2012)

      visible, with Ras85DV12.S35.UAS

      (Therrien et al., 1999)

      visible, with rprUAS.C

      (Dwivedi et al., 2015)

      visible | adult stage, with hepAct.UAS

      (Mlih and Karpac, 2022)

      visible | adult stage, with hidUAS.cIa

      (Dwivedi et al., 2015)

      visible | adult stage, with Hsap\TARDBPUAS.CO

      (Yusuff et al., 2023)

      visible | adult stage, with Tak1UAS.cMa

      (Ray and Lakhotia, 2019)
      Phenotype Manifest In

      cone cell | increased number, with KrnUAS.cUa

      (Brown and Freeman, 2003)

      cone cell | increased number, with prosL.UAS

      (Charlton-Perkins et al., 2011)

      cone cell | increased number, with svUAS.cKa

      (Charlton-Perkins et al., 2011)

      eye, with AlkUAS.cLa

      (Hugosson et al., 2014)

      eye, with BacA\p35UAS.cHa

      (Morris et al., 2006)

      eye, with cncUAS.cSa

      (Rahman et al., 2013)

      eye, with CycEUAS.cLa

      (Morris et al., 2006)

      eye, with FrlCA.UAS

      (Dollar et al., 2016)

      eye, with FrlDN.UAS

      (Dollar et al., 2016)

      eye, with grimUAS.cUa

      (Dwivedi et al., 2015)

      eye, with hepAct.UAS

      (Mlih and Karpac, 2022, Wang et al., 2005)

      eye, with hidUAS.cIa

      (Dwivedi et al., 2015)

      eye, with Hsap\TARDBPUAS.CO

      (Yusuff et al., 2023)

      eye, with Rac1N17.UAS

      (Morris et al., 2012)

      eye, with Ras85DV12.S35.UAS

      (Therrien et al., 1999)

      eye, with Ras85DV12.UAS

      (Ray and Lakhotia, 2019)

      eye, with rprUAS.C

      (Dwivedi et al., 2015)

      eye, with styUAS.cHa

      (Mao and Freeman, 2009)

      eye, with Tak1UAS.cMa

      (Ray and Lakhotia, 2019)

      eye disc | third instar larval stage, with FrlDN.UAS

      (Dollar et al., 2016)

      eye photoreceptor cell, with aPKCΔN.UAS

      (Djiane et al., 2005)

      eye photoreceptor cell, with Cdc42UAS.cWa

      (Dollar et al., 2016)

      eye photoreceptor cell, with FrlCA.UAS

      (Dollar et al., 2016)

      eye photoreceptor cell, with FrlDN.UAS

      (Dollar et al., 2016)

      interommatidial bristle, with panUAS.cWa

      (Jackson et al., 2002)

      medulla, with Liprin-βRNAi.UAS

      (Astigarraga et al., 2010)

      medulla, with trioGD9531

      (Astigarraga et al., 2010)

      ommatidial precursor cluster | third instar larval stage, with FrlDN.UAS

      (Dollar et al., 2016)

      ommatidium, with aPKCΔN.UAS

      (Djiane et al., 2005)

      ommatidium, with Cdc42UAS.cWa

      (Dollar et al., 2016)

      ommatidium, with cncUAS.cSa

      (Sykiotis and Bohmann, 2008)

      ommatidium, with FrlDN.UAS

      (Dollar et al., 2016)

      ommatidium, with KrnUAS.cUa

      (Brown and Freeman, 2003)

      photoreceptor cell R7, with Liprin-βRNAi.UAS

      (Astigarraga et al., 2010)

      photoreceptor cell R7, with trioGD9531

      (Astigarraga et al., 2010)

      primary pigment cell, with prosL.UAS

      (Charlton-Perkins et al., 2011)

      rhabdomere R7, with svUAS.cKa

      (Charlton-Perkins et al., 2011)

      rhabdomere R7 | increased number, with prosL.UAS

      (Charlton-Perkins et al., 2011)
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      NOT Enhanced by
      Suppressed by
      NOT suppressed by
      Statement
      Reference

      Scer\GAL4sev.EP, hepAct.UAS has visible | adult stage phenotype, non-suppressible by DiedelUAS.cMa, Scer\GAL4sev.EP

      (Mlih and Karpac, 2022)

      Rac1N17.UAS, Scer\GAL4sev.EP has visible phenotype, non-suppressible by CRMPsupK1

      (Morris et al., 2012)
      Enhancer of
      NOT Enhancer of
      Statement
      Reference

      KrnUAS.cUa/Scer\GAL4sev.EP is a non-enhancer of abnormal cell polarity phenotype of nmoP1

      (Brown and Freeman, 2003)
      Suppressor of
      NOT Suppressor of
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      FrlDN.UAS, Scer\GAL4sev.EP has ommatidium phenotype, enhanceable by Cdc421/Cdc42[+]

      (Dollar et al., 2016)

      FrlDN.UAS, Scer\GAL4sev.EP has eye photoreceptor cell phenotype, enhanceable by Cdc421/Cdc42[+]

      (Dollar et al., 2016)

      FrlDN.UAS, Scer\GAL4sev.EP has eye phenotype, enhanceable by Cdc421/Cdc42[+]

      (Dollar et al., 2016)

      FrlDN.UAS, Scer\GAL4sev.EP has ommatidium phenotype, enhanceable by Rac1V12.UAS/Scer\GAL4hs.2sev

      (Dollar et al., 2016)

      FrlDN.UAS, Scer\GAL4sev.EP has eye photoreceptor cell phenotype, enhanceable by Rac1V12.UAS/Scer\GAL4hs.2sev

      (Dollar et al., 2016)

      FrlDN.UAS, Scer\GAL4sev.EP has ommatidium phenotype, enhanceable by Df(2L)CadNΔ14/+

      (Dollar et al., 2016)

      FrlDN.UAS, Scer\GAL4sev.EP has ommatidium phenotype, enhanceable by Df(2L)BSC148/+

      (Dollar et al., 2016)

      Scer\GAL4sev.EP, cncUAS.cSa has eye phenotype, enhanceable by Scer\GAL4sev.EP/maf-SUAS.Tag:HA

      (Rahman et al., 2013)

      Scer\GAL4sev.EP, prosL.UAS has rhabdomere R7 | increased number phenotype, enhanceable by svspa-pol

      (Charlton-Perkins et al., 2011)

      Scer\GAL4sev.EP, svUAS.cKa has cone cell | increased number phenotype, enhanceable by Scer\GAL4sev.EP/prosRNAi.UAS

      (Charlton-Perkins et al., 2011)

      Scer\GAL4sev.EP, svUAS.cKa has rhabdomere R7 phenotype, enhanceable by Scer\GAL4sev.EP/prosRNAi.UAS

      (Charlton-Perkins et al., 2011)

      Scer\GAL4sev.EP, prosL.UAS has rhabdomere R7 | increased number phenotype, enhanceable by Scer\GAL4sev.EP/svRNAi.UAS

      (Charlton-Perkins et al., 2011)

      Scer\GAL4sev.EP, panUAS.cWa has interommatidial bristle phenotype, enhanceable by Hsap\MAPTGMR.Ex.PJ

      (Jackson et al., 2002)
      NOT Enhanced by
      Statement
      Reference

      FrlDN.UAS, Scer\GAL4sev.EP has ommatidium phenotype, non-enhanceable by nmoP1

      (Dollar et al., 2016)

      FrlDN.UAS, Scer\GAL4sev.EP has ommatidium phenotype, non-enhanceable by shg1

      (Dollar et al., 2016)

      FrlDN.UAS, Scer\GAL4sev.EP has ommatidium phenotype, non-enhanceable by shgk03401

      (Dollar et al., 2016)

      FrlDN.UAS, Scer\GAL4sev.EP has ommatidium phenotype, non-enhanceable by nmoDB

      (Dollar et al., 2016)

      AlkUAS.cLa, Scer\GAL4sev.EP has eye phenotype, non-enhanceable by miple1UAS.cHa, Scer\GAL4sev.EP

      (Hugosson et al., 2014)

      AlkUAS.cLa, Scer\GAL4sev.EP has eye phenotype, non-enhanceable by miple2UAS.Venus, Scer\GAL4sev.EP

      (Hugosson et al., 2014)

      AlkUAS.cLa, Scer\GAL4sev.EP has eye phenotype, non-enhanceable by Df(3L)miple1-2Δ104

      (Hugosson et al., 2014)

      Rac1N17.UAS, Scer\GAL4sev.EP has eye phenotype, non-enhanceable by CRMPsupK1

      (Morris et al., 2012)
      Suppressed by
      Statement
      Reference

      FrlDN.UAS, Scer\GAL4sev.EP has ommatidium phenotype, suppressible by Cdc42UAS.cWa, Scer\GAL4sev.EP

      (Dollar et al., 2016)

      FrlDN.UAS, Scer\GAL4sev.EP has eye phenotype, suppressible by Cdc42UAS.cWa, Scer\GAL4sev.EP

      (Dollar et al., 2016)

      Cdc42UAS.cWa, Scer\GAL4sev.EP has eye photoreceptor cell phenotype, suppressible | partially by Frl[+]/FrlEx83

      (Dollar et al., 2016)

      Cdc42UAS.cWa, Scer\GAL4sev.EP has eye photoreceptor cell phenotype, suppressible | partially by Frl[+]/FrlEx88

      (Dollar et al., 2016)

      Cdc42UAS.cWa, Scer\GAL4sev.EP has eye photoreceptor cell phenotype, suppressible | partially by Frl[+]/FrlExK62

      (Dollar et al., 2016)

      Cdc42UAS.cWa, Scer\GAL4sev.EP has eye photoreceptor cell phenotype, suppressible by FrlDN.UAS, Scer\GAL4sev.EP

      (Dollar et al., 2016)

      Scer\GAL4sev.EP, cncUAS.cSa has eye phenotype, suppressible | partially by maf-SRNAi.UAS/Scer\GAL4sev.EP

      (Rahman et al., 2013)

      Scer\GAL4sev.EP, prosL.UAS has cone cell | increased number phenotype, suppressible by Scer\GAL4sev.EP/svRNAi.UAS

      (Charlton-Perkins et al., 2011)

      Scer\GAL4sev.EP, prosL.UAS has cone cell | increased number phenotype, suppressible by svspa-pol

      (Charlton-Perkins et al., 2011)

      Scer\GAL4sev.EP, styUAS.cHa has eye phenotype, suppressible by Fas2EB112/Fas2[+]

      (Mao and Freeman, 2009)

      Scer\GAL4sev.EP, cncUAS.cSa has ommatidium phenotype, suppressible by Keap1UAS.cSa, Scer\GAL4sev.EP

      (Sykiotis and Bohmann, 2008)

      Scer\GAL4sev.EP, hepAct.UAS has eye phenotype, suppressible by foxo21/foxo[+]

      (Wang et al., 2005)
      NOT suppressed by
      Enhancer of
      NOT Enhancer of
      Suppressor of
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      rnroe.UAS/Scer\GAL4sev.EP partially rescues Df(3R)rn20/rn16

      (St Pierre et al., 2002)
      Fails to rescue

      Scer\GAL4sev.EP/rnUAS.cSPa fails to rescue Df(3R)rn20/rn16

      (St Pierre et al., 2002)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      Comments
      Comments

      The sev enhancer-promoter driver of P{sevEP-GAL4.B} is much more cell type-specific than the sev enhancer-heat-shock-promoter driver of the P{GAL4-Hsp70.sev} construct.

      (Bailey, 1999.12.9)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Scer\GAL4sev.EP
      Name Synonyms
      Secondary FlyBase IDs
        References (36)