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General Information
Symbol
Scer\GAL4P2.4.Pdf
Species
S. cerevisiae
Name
Pigment-dispersing factor promoter construct of Renn
FlyBase ID
FBal0102840
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
pdf-GAL4, pdfGAL4, pdfG4, pdf(M)-gal4, pdf, Pdf(BMRJ)-GAL4, Pdf-G4, P{PDF-Gal4}, Pdf-G
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

2.4kb of Pdf 5' flanking DNA regulates expression of a GAL4 driver.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

Drives expression in Pdf and LNv neurons.

In larval brain, expression in four cells in each hemisphere; these persist into adulthood and become the small ventrolateral neurons. In adult brain, also expressed in four large ventrolateral neurons, which emerge during metamorphosis. In both larval and adult stages, expressed in a small number of cells in the posterior-most CNS.

Expression of PdfUAS.cRa with Scer\GAL4P2.4.Pdf is weaker than wildtype Pdf expression, but expression is within the LNv neurons.

 
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

ventral adult lateral neuron & axon, with Abl::Hsap\ABL1::Hsap\BCRP210.UAS

ventral adult lateral neuron & axon, with AblUAS.cFa

ventral adult lateral neuron & axon, with hepCA.UAS

ventral adult lateral neuron & axon, with Hsap\APP695SPA4CT.UAS.Tag:MYC

ventral adult lateral neuron & axon, with Hsap\APPUAS.Tag:MYC

ventral adult lateral neuron & axon, with Hsap\APPΔGo.UAS.Tag:MYC

ventral adult lateral neuron & axon, with Hsap\APPΔNPTY.UAS.Tag:MYC

ventral adult lateral neuron & axon, with Hsap\APPΔPEER.UAS.Tag:MYC

ventral adult lateral neuron & commissure, with chicUAS.cCa

ventral adult lateral neuron & dendritic tree, with ApplUAS.cTa

Detailed Description
Statement
Reference

Elimination of molecular clock function (using cycΔ.UAS.Tag:MYC) in Scer\GAL4P2.4.Pdf cells in adults significantly reduces the strength of the circadian rhythm of sleep-wake cycles, compared to controls.

Inhibition (using Hsap\KCNJ2UAS.EGFP) of Scer\GAL4P2.4.Pdf cells significantly reduces the strength of the circadian rhythm of sleep-wake cycles, compared to controls.

Blocking clock function (using cycΔ.UAS.Tag:MYC) of Scer\GAL4P2.4.Pdf neurons significantly reduces the circadian rhythmicity of adult emergence after transfer to constant darkness, compared to controls.

Ablation of neurons expressing Scer\GAL4P2.4.Pdf has no effect on startle-induced climbing ability.

Optogenetic activation (using Crei\ChR2UAS.cSa with blue light activation) of Scer\GAL4P2.4.Pdf neurons in the larva results in excitatory junction potentials in anal sphincter muscles and strong contraction of anal sphincter muscles and other hindgut muscles, no excitation or contraction is seen due to blue light in controls.

Silencing (using Hsap\KCNJ2UAS.EGFP) of Scer\GAL4P2.4.Pdf neurons significantly increases the interval of defecation; when Scer\GAL4eve.RN2 neurons are additionally silenced, there is a significantly reduced frequency of defecation, compared to controls, with barely any hindgut movement.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
NOT Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
Enhancer of
NOT Enhancer of
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
Phenotype Manifest In
Enhanced by
NOT Enhanced by
Suppressed by
Statement
Reference

Hsap\APPUAS.Tag:MYC, Scer\GAL4P2.4.Pdf has ventral adult lateral neuron & axon phenotype, suppressible by Abl1/Abl[+]

Hsap\APPUAS.Tag:MYC, Scer\GAL4P2.4.Pdf has ventral adult lateral neuron & axon phenotype, suppressible by Abl4/Abl[+]

Hsap\APPUAS.Tag:MYC, Scer\GAL4P2.4.Pdf has ventral adult lateral neuron & axon phenotype, suppressible by AblK417N.UAS

Hsap\APPUAS.Tag:MYC, Scer\GAL4P2.4.Pdf has ventral adult lateral neuron & axon phenotype, suppressible by bskDN.UAS

Hsap\APPUAS.Tag:MYC, Scer\GAL4P2.4.Pdf has ventral adult lateral neuron & axon phenotype, suppressible by chic[+]/chic01320

Hsap\APPUAS.Tag:MYC, Scer\GAL4P2.4.Pdf has ventral adult lateral neuron & axon phenotype, suppressible by chic221

Hsap\APPUAS.Tag:MYC, Scer\GAL4P2.4.Pdf has ventral adult lateral neuron & axon phenotype, suppressible by kay1/kay[+]

Hsap\APPUAS.Tag:MYC, Scer\GAL4P2.4.Pdf has ventral adult lateral neuron & axon phenotype, suppressible | partially by JraIA109/Jra[+]

Scer\GAL4P2.4.Pdf, chicUAS.cCa has ventral adult lateral neuron & commissure phenotype, suppressible by Fmr1UAS.cZa, Scer\GAL4P2.4.Pdf

NOT suppressed by
Statement
Reference

Hsap\APPUAS.Tag:MYC, Scer\GAL4P2.4.Pdf has ventral adult lateral neuron & axon phenotype, non-suppressible by ena02029/ena[+]

Hsap\APPUAS.Tag:MYC, Scer\GAL4P2.4.Pdf has ventral adult lateral neuron & axon phenotype, non-suppressible by tsr[+]/tsrk05633

Scer\GAL4P2.4.Pdf, hepCA.UAS has ventral adult lateral neuron & axon phenotype, non-suppressible by Appld

AblUAS.cFa, Scer\GAL4P2.4.Pdf has ventral adult lateral neuron & axon phenotype, non-suppressible by bskDN.UAS

Abl::Hsap\ABL1::Hsap\BCRP210.UAS, Scer\GAL4P2.4.Pdf has ventral adult lateral neuron & axon phenotype, non-suppressible by Appld

Enhancer of
NOT Enhancer of
Suppressor of
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Scer\GAL4P2.4.Pdf
Scer\GAL4Pdf.PR
Name Synonyms
Pigment-dispersing factor promoter construct of Renn
Secondary FlyBase IDs
    References (207)