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General Information
Symbol
Dmel\PtenUAS.cHa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Huang
FlyBase ID
FBal0102916
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-PTEN, UAS-dPTEN
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of Pten isoform 2.

Expression of a 1.8kb Pten cDNA is governed by UASt regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

lipid droplet & larval oenocyte, with Scer\GAL4ppl.PP

Detailed Description
Statement
Reference

The expression of PtenScer\UAS.cHa under the control of Scer\GAL4E(spl)m6-BFM.PF leads to significantly decreased numbers of abdominal lateral and dorsal, but not ventral, adult muscle precursor cells in mid-third instar larvae, as compared to controls.

Expression of PtenScer\UAS.cHa under the control of Scer\GAL4kn-col85-GAL4 causes a reduction in the number of niche (posterior signalling center) cells in the lymph gland primary lobe and reduced differentiation of prohemocytes into plasmatocytes and crystal cells.

The number of filopodia at the leading edge is not significantly different from wild type in cells expressing PtenScer\UAS.cHa under the control of Scer\GAL4en.PU.

Embryos expressing PtenScer\UAS.cHa under the control of Scer\GAL4kirre.PU show a significant reduction in the number of nuclei in the segmental border muscle and in the DA1 muscle.

Central nervous system neuroblasts of fed larvae expressing PtenScer\UAS.cHa under the control of Scer\GAL4nab show a reduction in EdU incorporation compared to controls.

Overexpression of PtenScer\UAS.cHa in muscles under the control of Scer\GAL4Mhc.PW significantly preserves muscle protein homeostasis, while the muscles of wild-type animals display increased accumulation of protein aggregates.

Overexpression of PtenScer\UAS.cHa under the control of Scer\GAL4Mhc.PW in muscles is sufficient to significantly extend longevity compared with wild type by increasing the median and maximum life span of flies.

Ectopic expression of PtenScer\UAS.cHa under the control of Scer\GAL4ey.PH suppresses the growth of the developing eye.

Overexpression of PtenScer\UAS.cHa in the eye imaginal disc under the control of Scer\GAL4GMR.PF reduces the size of the eye.

Scer\GAL4ppk.PG-driven PtenScer\UAS.cHa-expression causes reduction in dendrite coverage of larval hemisegments.

Expression of PtenScer\UAS.cHa under the control of Scer\GAL4ppl.PP results in an accumulation of lipid droplets in the oenocytes of 89% of fed larvae (this contrasts with wild-type larvae, where oenocytes accumulate lipid droplets specifically under starvation conditions).

Overexpression of PtenScer\UAS.cHa, under the regulation of Scer\GAL4GMR.PF has little effect on the regular organisation of the ommatidia and photoreceptor number per ommatidium, although the overall size of the eye is moderately reduced.

When Scer\GAL4ppl.PP is driven by Scer\GAL4ppl.PP a reduction in fat body cell size and DNA endoreduplication is seen. No aggregation of storage vesicles in the fat body is seen. These flies are not delayed at eclosion nor reduced in size.

Expression of PtenScer\UAS.cHa under the control of Scer\GAL4GMR.PF results in eyes with decreased ommatidium size.

When expression is driven by Scer\GAL4ey.PH the eyes are reduced and flattened. In extreme cases no eye remains. Eye discs are reduced in size, though patterning and neural differentiation are normal. When expression is driven by Scer\GAL4GMR.PF photoreceptor cells have enlarged and irregularly shaped cell bodies and some pigment cells are missing. Adult eyes are reduced, flattened and roughened. The apical lenses are smaller though the photoreceptor cells are larger than wild type. Rhabdomeres are reduced in size. Eye discs show elevated cell death. When expression is driven by Scer\GAL4GMR.PF BrdU staining patterns are indistinguishable from wild type, but when expression is driven by Scer\GAL4ey.PH the number of staining cells in the anterior eye disc is reduced, indicating cell-cycle progression is reduced. The block does not act at the G1/S transition.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
NOT suppressed by
Statement
Reference
Suppressor of
NOT Suppressor of
Other
Phenotype Manifest In
Enhanced by
Suppressed by
NOT suppressed by
Enhancer of
Suppressor of
NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

The co-expression of dxScer\UAS.cMa suppresses the decreased number of abdominal lateral adult muscle precursor cells present in third instar larvae expressing PtenScer\UAS.cHa under the control of Scer\GAL4E(spl)m6-BFM.PF.

Overexpression of PtenScer\UAS.cHa driven by Scer\GAL4ppk.PU abolishes enhancement of axon regeneration seen in RtcaΔ/RtcaΔ larvae.

Expression of Arf79FScer\UAS.cKa does not suppress the niche (posterior signalling center) cell loss seen in the lymph gland primary lobe when PtenScer\UAS.cHa is expressed under the control of Scer\GAL4kn-col85-GAL4.

Expression of PtenScer\UAS.cHa does not suppress the niche (posterior signalling center) cell loss and increased differentiation of prohemocytes into plasmatocytes and crystal cells seen in the lymph gland when Arf79FGD12522 is expressed under the control of Scer\GAL4kn-col85-GAL4.

Expression of PtenScer\UAS.cHa dramatically suppresses the dendrite pruning defects seen in ddaC neurons expressing lin19GD9650 under the control of Scer\GAL4ppk.PG at 16 hours after pupariation.

Overexpression of PtenScer\UAS.cHa in subperineurial glia under the control of Scer\GAL4Gli-rL82 effectively normalizes the nerve phenotype in egh62d18, whereas the recruitment of plasmatocytes is suppressed.

Co-expression of S6kScer\UAS.cMa fails to suppress the reduction in growth of the developing eye seen upon expression of PtenScer\UAS.cHa under the control of Scer\GAL4ey.PH.

Ectopic expression of Hr46EY05451, combined with PtenScer\UAS.cHa, in the developing eye under the control of Scer\GAL4ey.PH, does not counteract the growth-suppressive effects of PtenScer\UAS.cHa expression.

Eye size in POSHScer\UAS.cSa egrScer\UAS.cMa (Scer\GAL4GMR.PF overexpression mutants is reduced upon co-expression of PtenScer\UAS.cHa.

The reduced dendrite coverage phenotype caused by Scer\GAL4ppk.PG-driven PtenScer\UAS.cHa-expression is epistatic to the dendrite overgrowth seen in Df(3L)banΔ1 mutants.

Coexpression of PtenScer\UAS.cHa with DJ-1αdsRNA.Scer\UAS, under the control of Scer\GAL4GMR.PF enhances the DJ-1αdsRNA.Scer\UAS-induced eye phenotype. The resulting eyes are dramatically reduced in size, with collapsed and fused ommatidia and necrotic spots, which are not present in DJ-1αdsRNA.Scer\UAS flies. There is also a near complete loss of photoreceptor neurons.

DJ-1αdsRNA.Scer\UAS flies coexpressing PtenScer\UAS.cHa (both in the dopaminergic neurons through Scer\GAL4ple.PF) exhibit higher levels of reactive oxygen species (ROS), compared to flies expressing DJ-1αdsRNA.Scer\UAS alone.

Expression of PtenScer\UAS.cHa in the eye under the control of Scer\GAL4GMR.PF does not suppress the phenotype of Tsc1Q600X mutant clones.

The eye phenotype caused by expression via Scer\GAL4ey.PH is suppressed by Pi3K92EScer\UAS.T:Hsap\MYC, enhanced by Pi3K92ED954A.Scer\UAS.T:Hsap\MYC and enhanced by reduction of chico as in chico1 heterozygotes.

Xenogenetic Interactions
Statement
Reference

Coexpression of PtenScer\UAS.cHa with Hsap\MAPTV337M.Scer\UAS, under the control of Scer\GAL4GMR.PF has no effect on the Hsap\MAPTV337M.Scer\UAS-induced toxicity in the eye.

Complementation and Rescue Data
Comments

Expression of PtenScer\UAS.cHa under the control of Scer\GAL4ppk.PG reduces the enhanced dendrite regeneration which is seen after severing of ddaC dendrites in PtenMGH1 mutant larvae.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
PtenScer\UAS.cHa
PtenUAS.cHa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Huang
Secondary FlyBase IDs
    References (32)