FB2025_01 , released February 20, 2025
Allele: Dmel\PtenUAS.cHa
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General Information
Symbol
Dmel\PtenUAS.cHa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Huang
FlyBase ID
FBal0102916
Feature type
allele
Associated gene
Dmel\Pten
Associated Insertion(s)
Carried in Construct
P{UAS-Pten.H}
Also Known As
UAS-PTEN, UAS-dPTEN
Key Links
Transgenic product class
cDNA
(Huang et al., 1999)
wild_type
(Huang et al., 1999)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Huang et al., 1999)
wild_type
(Huang et al., 1999)
Mutagen
in vitro construct
(Huang et al., 1999)
Progenitor genotype
Carried in construct
P{UAS-Pten.H}
Cytology
Description

UASt regulatory sequences drive expression of Pten isoform 2.

(von Stein et al., 2005)

Expression of a 1.8kb Pten cDNA is governed by UASt regulatory sequences.

(Huang et al., 1999)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Pten
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      abdominal dorsal adult muscle precursor cell | third instar larval stage, with Scer\GAL4E(spl)m6-BFM.PF

      (Aradhya et al., 2015)

      abdominal lateral adult muscle precursor cell | third instar larval stage, with Scer\GAL4E(spl)m6-BFM.PF

      (Aradhya et al., 2015)

      cell cycle, with Scer\GAL4ey.PH

      (Huang et al., 1999)

      dendrite | larval stage, with Scer\GAL4ppk.PG

      (Parrish et al., 2009)

      embryonic/larval crystal cell | third instar larval stage, with Scer\GAL4kn-col85-GAL4

      (Khadilkar et al., 2014)

      embryonic/larval fat body, with Scer\GAL4ppl.PP

      (Colombani et al., 2003)

      embryonic/larval plasmatocyte | third instar larval stage, with Scer\GAL4kn-col85-GAL4

      (Khadilkar et al., 2014)

      eye, with Scer\GAL4ey.PH

      (Montagne et al., 2010, Huang et al., 1999)

      eye, with Scer\GAL4GMR.PF

      (Tsuda et al., 2010, Yang et al., 2005, Huang et al., 1999)

      eye disc, with Scer\GAL4ey.PH

      (Huang et al., 1999)

      eye disc, with Scer\GAL4GMR.PF

      (Huang et al., 1999)

      eye disc posterior compartment | larval stage, with Scer\GAL4ey.PH

      (Huang et al., 1999)

      eye photoreceptor cell, with Scer\GAL4GMR.PF

      (Huang et al., 1999)

      larval neuroblast, with Scer\GAL4nab

      (Sousa-Nunes et al., 2011)

      lens, with Scer\GAL4GMR.PF

      (Huang et al., 1999)

      lipid droplet & larval oenocyte, with Scer\GAL4ppl.PP

      (Gutierrez et al., 2007)

      muscle cell, with Scer\GAL4Mhc.PW

      (Demontis and Perrimon, 2010)

      muscle cell of A1-7 dorsal acute muscle 1, with Scer\GAL4kirre.PU

      (Tixier et al., 2013)

      ommatidium, with Scer\GAL4ey.PH

      (Huang et al., 1999)

      ommatidium, with Scer\GAL4GMR.PF

      (Potter et al., 2001)

      photoreceptor neuron | adult stage, with Scer\GAL4GMR.PF

      (Huang et al., 1999)

      pigment cell, with Scer\GAL4GMR.PF

      (Huang et al., 1999)

      posterior signaling center of lymph gland primary lobe | third instar larval stage, with Scer\GAL4kn-col85-GAL4

      (Khadilkar et al., 2014)

      rhabdomere, with Scer\GAL4GMR.PF

      (Huang et al., 1999)

      segment border muscle cell, with Scer\GAL4kirre.PU

      (Tixier et al., 2013)
      Detailed Description
      Statement
      Reference

      The expression of PtenScer\UAS.cHa under the control of Scer\GAL4E(spl)m6-BFM.PF leads to significantly decreased numbers of abdominal lateral and dorsal, but not ventral, adult muscle precursor cells in mid-third instar larvae, as compared to controls.

      (Aradhya et al., 2015)

      Expression of PtenScer\UAS.cHa under the control of Scer\GAL4kn-col85-GAL4 causes a reduction in the number of niche (posterior signalling center) cells in the lymph gland primary lobe and reduced differentiation of prohemocytes into plasmatocytes and crystal cells.

      (Khadilkar et al., 2014)

      The number of filopodia at the leading edge is not significantly different from wild type in cells expressing PtenScer\UAS.cHa under the control of Scer\GAL4en.PU.

      (Pickering et al., 2013)

      Embryos expressing PtenScer\UAS.cHa under the control of Scer\GAL4kirre.PU show a significant reduction in the number of nuclei in the segmental border muscle and in the DA1 muscle.

      (Tixier et al., 2013)

      Central nervous system neuroblasts of fed larvae expressing PtenScer\UAS.cHa under the control of Scer\GAL4nab show a reduction in EdU incorporation compared to controls.

      (Sousa-Nunes et al., 2011)

      Overexpression of PtenScer\UAS.cHa in muscles under the control of Scer\GAL4Mhc.PW significantly preserves muscle protein homeostasis, while the muscles of wild-type animals display increased accumulation of protein aggregates.

      Overexpression of PtenScer\UAS.cHa under the control of Scer\GAL4Mhc.PW in muscles is sufficient to significantly extend longevity compared with wild type by increasing the median and maximum life span of flies.

      (Demontis and Perrimon, 2010)

      Ectopic expression of PtenScer\UAS.cHa under the control of Scer\GAL4ey.PH suppresses the growth of the developing eye.

      (Montagne et al., 2010)

      Overexpression of PtenScer\UAS.cHa in the eye imaginal disc under the control of Scer\GAL4GMR.PF reduces the size of the eye.

      (Tsuda et al., 2010)

      Scer\GAL4ppk.PG-driven PtenScer\UAS.cHa-expression causes reduction in dendrite coverage of larval hemisegments.

      (Parrish et al., 2009)

      Expression of PtenScer\UAS.cHa under the control of Scer\GAL4ppl.PP results in an accumulation of lipid droplets in the oenocytes of 89% of fed larvae (this contrasts with wild-type larvae, where oenocytes accumulate lipid droplets specifically under starvation conditions).

      (Gutierrez et al., 2007)

      Overexpression of PtenScer\UAS.cHa, under the regulation of Scer\GAL4GMR.PF has little effect on the regular organisation of the ommatidia and photoreceptor number per ommatidium, although the overall size of the eye is moderately reduced.

      (Yang et al., 2005)

      When Scer\GAL4ppl.PP is driven by Scer\GAL4ppl.PP a reduction in fat body cell size and DNA endoreduplication is seen. No aggregation of storage vesicles in the fat body is seen. These flies are not delayed at eclosion nor reduced in size.

      (Colombani et al., 2003)

      Expression of PtenScer\UAS.cHa under the control of Scer\GAL4GMR.PF results in eyes with decreased ommatidium size.

      (Potter et al., 2001)

      When expression is driven by Scer\GAL4ey.PH the eyes are reduced and flattened. In extreme cases no eye remains. Eye discs are reduced in size, though patterning and neural differentiation are normal. When expression is driven by Scer\GAL4GMR.PF photoreceptor cells have enlarged and irregularly shaped cell bodies and some pigment cells are missing. Adult eyes are reduced, flattened and roughened. The apical lenses are smaller though the photoreceptor cells are larger than wild type. Rhabdomeres are reduced in size. Eye discs show elevated cell death. When expression is driven by Scer\GAL4GMR.PF BrdU staining patterns are indistinguishable from wild type, but when expression is driven by Scer\GAL4ey.PH the number of staining cells in the anterior eye disc is reduced, indicating cell-cycle progression is reduced. The block does not act at the G1/S transition.

      (Huang et al., 1999)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Suppressed by
      NOT suppressed by
      Statement
      Reference

      PtenUAS.cHa, Scer\GAL4ey.PH has visible phenotype, non-suppressible by BacA\p35UAS.cHa, Scer\GAL4ey.PH

      (Huang et al., 1999)
      Suppressor of
      NOT Suppressor of
      Statement
      Reference

      PtenUAS.cHa/Scer\GAL4GMR.PF is a non-suppressor of visible | somatic clone phenotype of Tsc1Q600X

      (Potter et al., 2001)

      PtenUAS.cHa/Scer\GAL4GMR.PF is a non-suppressor of abnormal size | somatic clone | adult stage phenotype of Tsc1Q600X

      (Potter et al., 2001)
      Other
      Phenotype Manifest In
      Enhanced by
      Suppressed by
      NOT suppressed by
      Enhancer of
      Statement
      Reference

      PtenUAS.cHa, Scer\GAL4GMR.PF is an enhancer of eye phenotype of Scer\GAL4GMR.PF, egrUAS.cMa

      (Tsuda et al., 2010)

      PtenUAS.cHa, Scer\GAL4GMR.PF is an enhancer of eye phenotype of DJ-1αRNAi.UAS, Scer\GAL4GMR.PF

      (Yang et al., 2005)
      Suppressor of
      NOT Suppressor of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The co-expression of dxScer\UAS.cMa suppresses the decreased number of abdominal lateral adult muscle precursor cells present in third instar larvae expressing PtenScer\UAS.cHa under the control of Scer\GAL4E(spl)m6-BFM.PF.

      (Aradhya et al., 2015)

      Overexpression of PtenScer\UAS.cHa driven by Scer\GAL4ppk.PU abolishes enhancement of axon regeneration seen in RtcaΔ/RtcaΔ larvae.

      (Song et al., 2015)

      Expression of Arf79FScer\UAS.cKa does not suppress the niche (posterior signalling center) cell loss seen in the lymph gland primary lobe when PtenScer\UAS.cHa is expressed under the control of Scer\GAL4kn-col85-GAL4.

      Expression of PtenScer\UAS.cHa does not suppress the niche (posterior signalling center) cell loss and increased differentiation of prohemocytes into plasmatocytes and crystal cells seen in the lymph gland when Arf79FGD12522 is expressed under the control of Scer\GAL4kn-col85-GAL4.

      (Khadilkar et al., 2014)

      Expression of PtenScer\UAS.cHa dramatically suppresses the dendrite pruning defects seen in ddaC neurons expressing lin19GD9650 under the control of Scer\GAL4ppk.PG at 16 hours after pupariation.

      (Wong et al., 2013)

      Overexpression of PtenScer\UAS.cHa in subperineurial glia under the control of Scer\GAL4Gli-rL82 effectively normalizes the nerve phenotype in egh62d18, whereas the recruitment of plasmatocytes is suppressed.

      (Dahlgaard et al., 2012)

      Co-expression of S6kScer\UAS.cMa fails to suppress the reduction in growth of the developing eye seen upon expression of PtenScer\UAS.cHa under the control of Scer\GAL4ey.PH.

      Ectopic expression of Hr46EY05451, combined with PtenScer\UAS.cHa, in the developing eye under the control of Scer\GAL4ey.PH, does not counteract the growth-suppressive effects of PtenScer\UAS.cHa expression.

      (Montagne et al., 2010)

      Eye size in POSHScer\UAS.cSa egrScer\UAS.cMa (Scer\GAL4GMR.PF overexpression mutants is reduced upon co-expression of PtenScer\UAS.cHa.

      (Tsuda et al., 2010)

      The reduced dendrite coverage phenotype caused by Scer\GAL4ppk.PG-driven PtenScer\UAS.cHa-expression is epistatic to the dendrite overgrowth seen in Df(3L)banΔ1 mutants.

      (Parrish et al., 2009)

      Coexpression of PtenScer\UAS.cHa with DJ-1αdsRNA.Scer\UAS, under the control of Scer\GAL4GMR.PF enhances the DJ-1αdsRNA.Scer\UAS-induced eye phenotype. The resulting eyes are dramatically reduced in size, with collapsed and fused ommatidia and necrotic spots, which are not present in DJ-1αdsRNA.Scer\UAS flies. There is also a near complete loss of photoreceptor neurons.

      (Yang et al., 2005)

      DJ-1αdsRNA.Scer\UAS flies coexpressing PtenScer\UAS.cHa (both in the dopaminergic neurons through Scer\GAL4ple.PF) exhibit higher levels of reactive oxygen species (ROS), compared to flies expressing DJ-1αdsRNA.Scer\UAS alone.

      (Yang, 2005)

      Expression of PtenScer\UAS.cHa in the eye under the control of Scer\GAL4GMR.PF does not suppress the phenotype of Tsc1Q600X mutant clones.

      (Potter et al., 2001)

      The eye phenotype caused by expression via Scer\GAL4ey.PH is suppressed by Pi3K92EScer\UAS.T:Hsap\MYC, enhanced by Pi3K92ED954A.Scer\UAS.T:Hsap\MYC and enhanced by reduction of chico as in chico1 heterozygotes.

      (Huang et al., 1999)
      Xenogenetic Interactions
      Statement
      Reference

      Coexpression of PtenScer\UAS.cHa with Hsap\MAPTV337M.Scer\UAS, under the control of Scer\GAL4GMR.PF has no effect on the Hsap\MAPTV337M.Scer\UAS-induced toxicity in the eye.

      (Yang et al., 2005)
      Complementation and Rescue Data
      Rescues

      PtenUAS.cHa/Scer\GAL4ppk.PG rescues PtenMGH1

      (Song et al., 2012)
      Comments

      Expression of PtenScer\UAS.cHa under the control of Scer\GAL4ppk.PG reduces the enhanced dendrite regeneration which is seen after severing of ddaC dendrites in PtenMGH1 mutant larvae.

      (Song et al., 2012)
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      PtenScer\UAS.cHa
      PtenUAS.cHa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Huang
      (Huang et al., 1999)
      Secondary FlyBase IDs
        References (32)