Allele Dmel\Klp64D^k5

General Information
Symbol	Dmel\Klp64D^k5	Species	D. melanogaster
Name		FlyBase ID	FBal0102963
Feature type	allele	Associated gene	Dmel\Klp64D

Map ( GBrowse )
Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Ray et al., 1999)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 3L:5,350,704..5,350,704 evidence=experimental pr_change=E551K\|Klp64D-PA reported_na_change=G?A reported_pr_change=E551K na_change=G5350704A (Ray et al., 1999)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: E551K. (Ray et al., 1999)
Cytology
Phenotypic Data
Phenotypic Class
	auditory perception defective (with Klp64D^k1) (Sarpal et al., 2003) auditory perception defective (with Klp64D^l4) (Sarpal et al., 2003) auditory perception defective \| recessive (Sarpal et al., 2003) hypoactive \| recessive (Ray et al., 1999) lethal \| recessive (Ray et al., 1999) neuroanatomy defective (Jana et al., 2011) neuroanatomy defective (with Klp64D^k1) (Jana et al., 2011) neuroanatomy defective (with Klp64D^l4) (Jana et al., 2011) neurophysiology defective (with Klp64D^k1) (Sarpal et al., 2003, Jana et al., 2011) neurophysiology defective (with Klp64D^l4) (Sarpal et al., 2003, Jana et al., 2011) neurophysiology defective \| recessive (Sarpal et al., 2003, Jana et al., 2011) uncoordinated \| adult stage \| recessive (Ray et al., 1999)
Phenotype Manifest In
	cilium (Jana et al., 2011) cilium (with Klp64D^k1) (Jana et al., 2011) cilium (with Klp64D^l4) (Jana et al., 2011) Johnston's organ & scolopidial cilium (Sarpal et al., 2003) Johnston organ (with Klp64D^l4) (Sarpal et al., 2003) scolopidial cilium & Johnston's organ (with Klp64D^l4) (Sarpal et al., 2003) sensillum basiconicum of antennal segment 3 (Jana et al., 2011) sensillum basiconicum of antennal segment 3 (with Klp64D^k1) (Jana et al., 2011) sensillum basiconicum of antennal segment 3 (with Klp64D^l4) (Jana et al., 2011)
Detailed Description
	Statement Reference The average peak electroantennogram response is reduced in homozygous, Klp64D[k5]/Klp64D[l4] and Klp64D[k5]/Klp64D[k1] adults compared to controls (in response to ethyl acetate, n-butanol, benzaldehyde or propionic acid). The electron-dense basal bodies and the connecting cilium are often present in the sensilla basiconica of the antenna in mutant adults, but the outer segments of the basiconic cilia are less branched than in wild type. The electron-dense basal bodies and the connecting cilium are often present in the sensilla basiconica of the antenna in Klp64D[k5]/Klp64D[l4] adults, but the outer segments of the basiconic cilia usually contain a slender extension with several singlet microtubules. The outer segments of the basiconic cilia of the sensilla basiconica of the antenna are less branched than normal in Klp64D[k5]/Klp64D[k1] adults. (Jana et al., 2011) Klp64D^k5 homozygotes, Klp64D^k1/Klp64D^k5, and Klp64D^l4/Klp64D^k5 animals have a reduced auditory response, producing a severely reduced sound evoked potentials from the Johnston's organ neurons. The Johnston organ scolopidia contains complete sets of cilia. The basal body structures, ciliary roots and desmosomal junctions between inner dendritic segments appear normal. However, the ciliary dilations are deformed and disorganised and are often located in the same plane as the dendritic caps. (Sarpal et al., 2003) Homozygotes die at or before the early third larval instar stage in a typical crowded culture. If the homozygotes are raised in low density conditions without competition from wild-type larvae, a few mutant animals survive to be pharate pupae and occasionally eclose as fully formed adults. The adults show an acutely uncoordinated walk, are unable to stand and die in a few hours. Mutant third instar larvae show slight to severe sluggishness and roll abnormally from side to side during crawling. (Ray et al., 1999)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Klp64D^k5/Klp64D^l4 has auditory perception defective phenotype, enhanceable by Kap3^V6 (Sarpal et al., 2003) Klp64D^k5/Klp64D^l4 has neurophysiology defective phenotype, enhanceable by Kap3^V6 (Sarpal et al., 2003)
Suppressor of
Statement Reference Klp64D^k5 is a suppressor of paralytic \| posterior \| larval stage phenotype of Aplip1^EK4 (Horiuchi et al., 2005)
Other
Statement Reference Kap3^V6, Klp64D^k5/Klp64D^l4 has lethal \| partially phenotype (Sarpal et al., 2003, Sarpal et al., 2003) Kap3^V6, Klp64D^k5/Klp64D^l4 has lethal phenotype (Sarpal et al., 2003, Sarpal et al., 2003)
Phenotype Manifest In
Enhanced by
Statement Reference Klp64D^k5/Klp64D^l4 has Johnston organ phenotype, enhanceable by Kap3^V6 (Sarpal et al., 2003) Klp64D^k5/Klp64D^l4 has scolopidial cilium & Johnston's organ phenotype, enhanceable by Kap3^V6 (Sarpal et al., 2003)
Suppressor of
Statement Reference Klp64D^k5 is a suppressor of axon phenotype of Aplip1^EK4 (Horiuchi et al., 2005)
Additional Comments
Genetic Interactions
Statement Reference In Aplip1^EK4, Klp64D^k5 double mutants the posterior paralysis and axonal swelling of Aplip1^EK4 single mutants are suppressed. (Horiuchi et al., 2005) The addition of Kap3^V6/+ significantly enhances the recessive lethality of Klp64D^l4/Klp64D^k5 animals. The addition of Kap3^V6/Y causes complete lethality. The addition of Kap3^V6/+ to Klp64D^l4/Klp64D^k5 also completely abolishes the sound evoked potentials usually obtained from the Johnston's organ neurons, and enhances the ciliary defects seen in these animals. (Sarpal et al., 2003)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	Klp64Dⁿ¹²³ (Ray et al., 1999)
Rescued by	Klp64D^k5/Klp64D^k1 is rescued by Scer\GAL4^da.G32/Klp64D^Scer\UAS.cRa (Jana et al., 2011) Klp64D^k5/Klp64Dⁿ¹²³ is rescued by Scer\GAL4[-]/Klp64D^Scer\UAS.cRa (Ray et al., 1999) Klp64D^k5 is rescued by Scer\GAL4[-]/Klp64D^Scer\UAS.cRa (Ray et al., 1999) Klp64D^k5 is rescued by Scer\GAL4^JO15/Klp64D^Scer\UAS.cRa (Sarpal et al., 2003) Klp64D^k5 is rescued by Scer\GAL4^MJ94/Klp64D^Scer\UAS.cRa (Sarpal et al., 2003) Klp64D^k5 is rescued by Scer\GAL4^Tm1-C817/Klp64D^Scer\UAS.cRa (Sarpal et al., 2003)
Comments	Expression of Klp64D[Scer\UAS.cRa] under the control of Scer\GAL4[da.G32] rescues the reduced electroantennogram response of Klp64D[k5]/Klp64D[k1] adults in response to ethyl acetate, n-butanol, benzaldehyde or propionic acid. (Jana et al., 2011) Klp64D^Scer\UAS.cRa rescues the lethality and uncoordinated behaviour of Klp64D^k5, either in homozygous condition or in transheterozygous combination with Klp64Dⁿ¹²³, in the absence of a Scer\GAL4 driver. (Ray et al., 1999)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Klp64D^k5 (Baqri et al., 2006, Mattie et al., 2010, Jana et al., 2011)
Name Synonym
Secondary FlyBase IDs

References ( 6 )
Research paper	Jana et al., 2011, Mol. Biol. Cell 22(6): 769--781 Heterotrimeric kinesin-II is necessary and sufficient to promote different stepwise assembly of morphologically distinct bipartite cilia in Drosophila antenna. [FBrf0213262] Mattie et al., 2010, Curr. Biol. 20(24): 2169--2177 Directed Microtubule Growth, +TIPs, and Kinesin-2 Are Required for Uniform Microtubule Polarity in Dendrites. [FBrf0212550] Baqri et al., 2006, J. Neurobiol. 66(4): 378--392 Kinesin-2 differentially regulates the anterograde axonal transports of acetylcholinesterase and choline acetyltransferase in Drosophila. [FBrf0191019] Horiuchi et al., 2005, Curr. Biol. 15(23): 2137--2141 APLIP1, a kinesin binding JIP-1/JNK scaffold protein, influences the axonal transport of both vesicles and mitochondria in Drosophila. [FBrf0190020] Sarpal et al., 2003, Curr. Biol. 13(19): 1687--1696 Drosophila KAP interacts with the kinesin II motor subunit KLP64D to assemble chordotonal sensory cilia, but not sperm tails. [FBrf0167402] Ray et al., 1999, J. Cell Biol. 147(3): 507--518 Kinesin-II is required for axonal transport of choline acetyltransferase in Drosophila. [FBrf0112015]

Allele Dmel\Klp64Dk5

Allele Dmel\Klp64D^k5