|Feature type||allele||Associated gene||Dmel\Klp64D|
|Map ( GBrowse )|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
Amino acid replacement: E551K.
|Phenotype Manifest In|
Johnston's organ & scolopidial cilium
The average peak electroantennogram response is reduced in homozygous, Klp64D[k5]/Klp64D[l4] and Klp64D[k5]/Klp64D[k1] adults compared to controls (in response to ethyl acetate, n-butanol, benzaldehyde or propionic acid). The electron-dense basal bodies and the connecting cilium are often present in the sensilla basiconica of the antenna in mutant adults, but the outer segments of the basiconic cilia are less branched than in wild type. The electron-dense basal bodies and the connecting cilium are often present in the sensilla basiconica of the antenna in Klp64D[k5]/Klp64D[l4] adults, but the outer segments of the basiconic cilia usually contain a slender extension with several singlet microtubules. The outer segments of the basiconic cilia of the sensilla basiconica of the antenna are less branched than normal in Klp64D[k5]/Klp64D[k1] adults.
Klp64Dk5 homozygotes, Klp64Dk1/Klp64Dk5, and Klp64Dl4/Klp64Dk5 animals have a reduced auditory response, producing a severely reduced sound evoked potentials from the Johnston's organ neurons. The Johnston organ scolopidia contains complete sets of cilia. The basal body structures, ciliary roots and desmosomal junctions between inner dendritic segments appear normal. However, the ciliary dilations are deformed and disorganised and are often located in the same plane as the dendritic caps.
Homozygotes die at or before the early third larval instar stage in a typical crowded culture. If the homozygotes are raised in low density conditions without competition from wild-type larvae, a few mutant animals survive to be pharate pupae and occasionally eclose as fully formed adults. The adults show an acutely uncoordinated walk, are unable to stand and die in a few hours. Mutant third instar larvae show slight to severe sluggishness and roll abnormally from side to side during crawling.
|Phenotype Manifest In|
In Aplip1EK4, Klp64Dk5 double mutants the posterior paralysis and axonal swelling of Aplip1EK4 single mutants are suppressed.
The addition of Kap3V6/+ significantly enhances the recessive lethality of Klp64Dl4/Klp64Dk5 animals. The addition of Kap3V6/Y causes complete lethality. The addition of Kap3V6/+ to Klp64Dl4/Klp64Dk5 also completely abolishes the sound evoked potentials usually obtained from the Johnston's organ neurons, and enhances the ciliary defects seen in these animals.
|Complementation & Rescue Data|
|Fails to complement|
Expression of Klp64D[Scer\UAS.cRa] under the control of Scer\GAL4[da.G32] rescues the reduced electroantennogram response of Klp64D[k5]/Klp64D[k1] adults in response to ethyl acetate, n-butanol, benzaldehyde or propionic acid.
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 1 )|
|Secondary FlyBase IDs|
|References ( 6 )|