FlyBase Allele Report: Dmel\InR[UAS.cHa]

Under control nutrition conditions, the adulthood-only expression of InR^UAS.cHa under the combined control of Scer\GAL4^C587 and Gal80[ts] does not lead to any significant changes in the number of escort cells in the germarium, as compared to controls.

(Su et al., 2018)

Expression of InR^Scer\UAS.cHa under the control of Scer\GAL4^phm.PO does not significantly change the number of lipid droplets in the prothoracic gland cells of third instar larvae compared to controls.

(Danielsen et al., 2016)

Wild type males spend significantly more courtship time with females expressing InR^Scer\UAS.cHa under the control of Scer\GAL4^{αTub84B.Switch.PK} (upon exposure to RU486), as compared to control females. The same effect on female attractiveness is seen when cuticular hydrocarbons from the aforementioned females are used to "perfume" same-age oenocyte-less flies.

(Kuo et al., 2012)

Expression of InR^Scer\UAS.cHa under the control of Scer\GAL4^GMR.PF does not produce R8 photoreceptor cell phenotypes.

(Mann et al., 2012)

Overexpression of InR^Scer\UAS.cHa in fat body cell clones under the control of Scer\GAL4^αTub84B.PP leads to an enormous fat body cell overgrowth phenotype. This is accompanied by a significant reduction in lipid droplet size compared with wild-type.

(Tiefenböck et al., 2010)

Fat body clones expressing InR^Scer\UAS.cHa under the control of Scer\GAL4^αTub84B.PP show an increase in cell size.

(Baltzer et al., 2009)

Overexpression of InR^Scer\UAS.cHa under the control of Scer\GAL4^GMR.PF results in eye hyperplasia.

(Honegger et al., 2008)

Expression of InR^Scer\UAS.cHa under the control of Scer\GAL4^GMR.PF leads to an increase in ommatidia size.

(Xu et al., 2008)

Expression of InR^Scer\UAS.cHa under the control of Scer\GAL4^ptc-559.1 results in a weak but consistent expansion of the distance between wing veins L3 and L4.

(Hsu et al., 2007)

Expression of InR^Scer\UAS.cHa under the control of Scer\GAL4^tim.Scer\UAS, dramatically increases sensitivity to oxidative stress. These flies die within 2 days of being challenged with 1mM paraquat. When treated with 0.05-0.1mM paraquat they survive up to 6 days, with the majority showing arrhythmic locomotor behavior.

Expression of InR^Scer\UAS.cHa under the control of Scer\GAL4^Switch1.106, increases sensitivity of behavioral rhythms to paraquat.

(Zheng et al., 2007)

Expression of InR^Scer\UAS.cHa in border cells, driven by Scer\GAL4^slbo.2.6 does not affect the formation of the egg chamber apical cap or the migration of the border cell cluster to the oocyte.

(Medioni and Noselli, 2005)

Pharate adults expressing InR^Scer\UAS.cHa under the control of Scer\GAL4^phm.PO are 42% of the weight of controls when larvae have been raised in constant light.

(Mirth et al., 2005)

Expression of InR^Scer\UAS.cHa under the control of Scer\GAL4^dpp.blk1 results in ectopic bristles and an excess of sensilla trichodea in the most proximal part of the coxa, ectopic bristles in the arista and third antennal segment, ectopic bristles in the capitellum and occasionally in the pedicellum of the haltere and ectopic thorn bristles in the female genitalia.

(Sustar and Schubiger, 2005)

Cells in InR^Scer\UAS.cHa; Scer\GAL4^Act5C.PP somatic clonesi n the larval fat body overgrow dramatically.

(Wang et al., 2005)

The eyes of InR^Scer\UAS.cHa; Scer\GAL4^GMR.PF flies have massive overgrowth.

(Wittwer et al., 2005)

Flies that express InR^Scer\UAS.cHa under the control of both Scer\GAL4^tin.cBa and Scer\GAL4^Scer\UAS.cHa show a consistently low heart rate across all ages compared to wild-type flies. These flies show an increased rate of stress-induced heart failure at the age of one week compared to in wild type. The rate of this heart failure does increase with age, but at a markedly reduced magnitude compared to wild type. These effects are true for both male and female.

(Wessells et al., 2004)

Almost all endogenous caspase activation is blocked in InR^Scer\UAS.cHa; Scer\GAL4^GMR.PF eye discs of third instar larvae and 30 hours APF pupae. This is accompanied by an increase in photoreceptor differentiation at both of these stages.

(Yang and Baker, 2003)

Clones of cells expressing InR^Scer\UAS.cHa under the control of Scer\GAL4^Act5C.PP in the gut, fat body, Malpighian tubules and epidermis are only slightly larger than control cells at the time of larval hatching. After several days of starvation on 20% sucrose, these cells are much larger than adjacent control cells and have a visibly increased DNA content. Gut and fat body cells expressing InR^Scer\UAS.cHa under the control of Scer\GAL4^Act5C.PP continue to replicate their DNA for at least 2-3 days under starvation conditions. Expression of InR^Scer\UAS.cHa in third larval instar fat body cells under the control of Scer\GAL4^Act5C.PP increases the opacity of the cytoplasm, thus promoting nutrient storage. The cytoplasm of the cells contains many more, but much smaller, lipid droplets than neighbouring control cells. Some fed animals expressing InR^Scer\UAS.cHa under the control of Scer\GAL4^Adh.PF survive to the third larval instar and a few viable adults eclose. In contrast, expression of InR^Scer\UAS.cHa under the control of Scer\GAL4^Adh.PF in starved animals results in first instar larvae dying within 3-4 days of hatching (control animals survive 8-9 days). Animals expressing InR^Scer\UAS.cHa under the control of Scer\GAL4^Adh.PF feed poorly, often having no food in their gut, and frequently wander away from their food.

(Britton et al., 2002)

Expression of InR^Scer\UAS.cHa under the control of Scer\GAL4^Act5C.PP in clones results in an increase in cell size and an increase in clone area compared to controls.

(Prober and Edgar, 2002)

InR^Scer\UAS.cHa when driven by Scer\GAL4^ey.PH causes hyperproliferative outgrowth and an increase in cell size in the eye. A relatively normal ommatidial arrangement and architecture are retained.

(Brogiolo et al., 2001)

When InR^Scer\UAS.cHa is driven by Scer\GAL4^GMR.PF the flies have enlarged eyes due to an increase in cell number and cell size.

(Morey et al., 2001)

Expression of InR^Scer\UAS.cHa under the control of Scer\GAL4^ey.PH results in lethality at 25^oC and a dramatic increase in ommatidia number in escapers at room temperature.

(Potter et al., 2001)

When expression is driven by Scer\GAL4^ey.PH at 25^oC lethality results. At room temperature, a few flies survive with over proliferated eyes.

(Huang et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

InR^UAS.cHa, Scer\GAL4^GMR.PF has visible phenotype, enhanceable by Pdk1^4ΔEP/Pdk1^EP3091, Scer\GAL4^GMR.PF

(Rintelen et al., 2001)

NOT Enhanced by

Statement

Reference

InR^UAS.cHa, Scer\GAL4^αTub84B.PP has increased cell size | somatic clone phenotype, non-enhanceable by Ets97D⁶¹³/Df(3R)ro80b

(Baltzer et al., 2009)

Suppressed by

Statement

Reference

InR^UAS.cHa, Scer\GAL4^αTub84B.PP has increased cell size | somatic clone phenotype, suppressible by srl^KG08646

(Tiefenböck et al., 2010)

InR^UAS.cHa, Scer\GAL4^GMR.PF has visible phenotype, suppressible by ImpL2^EP5.66, Scer\GAL4^GMR.PF

(Honegger et al., 2008)

InR^UAS.cHa, Scer\GAL4^GMR.PF has visible phenotype, suppressible | partially by ImpL2^UAS.cHa, Scer\GAL4^GMR.PF

(Honegger et al., 2008)

InR^UAS.cHa, Scer\GAL4^GMR.PF has visible phenotype, suppressible by ImpL2^s.UAS, Scer\GAL4^GMR.PF

(Honegger et al., 2008)

InR^UAS.cHa, Scer\GAL4^Act5C.PP has increased cell size | somatic clone phenotype, suppressible | partially by hep^Act.UAS, Scer\GAL4^Act5C.PP

(Wang et al., 2005)

InR^UAS.cHa, Scer\GAL4^GMR.PF has hyperplasia phenotype, suppressible by B4^EP7-66

(Wittwer et al., 2005)

InR^UAS.cHa, Scer\GAL4^GMR.PF has hyperplasia phenotype, suppressible by B4^UAS.cSa

(Wittwer et al., 2005)

InR^UAS.cHa, Scer\GAL4^en-e16E has lethal | embryonic stage phenotype, suppressible by B4^UAS.cSa

(Wittwer et al., 2005)

InR^UAS.cHa, Scer\GAL4^GMR.PB has visible phenotype, suppressible by Df(3L)AC1

(Brogiolo et al., 2001)

InR^UAS.cHa, Scer\GAL4^ey.PH has visible phenotype, suppressible by gig^UAS.cTa/Tsc1^UAS.cPa, Scer\GAL4^ey.PH

(Potter et al., 2001)

InR^UAS.cHa, Scer\GAL4^ey.PH has lethal | heat sensitive phenotype, suppressible by gig^UAS.cTa/Tsc1^UAS.cPa, Scer\GAL4^ey.PH

(Potter et al., 2001)

InR^UAS.cHa, Scer\GAL4^GMR.PF has visible phenotype, suppressible by Pdk1^4ΔEP/Pdk1^EP3091, Scer\GAL4^GMR.PF

(Rintelen et al., 2001)

InR^UAS.cHa, Scer\GAL4^GMR.PF has visible phenotype, suppressible by Pk61C[+]/Pdk1^5ΔEP, Scer\GAL4^GMR.PF

(Rintelen et al., 2001)

InR^UAS.cHa, Scer\GAL4^ey.PH has abnormal size | adult stage phenotype, suppressible by Pten^UAS.cHa, Scer\GAL4^ey.PH

(Huang et al., 1999)

NOT suppressed by

Statement

Reference

InR^UAS.cHa, Scer\GAL4^αTub84B.PP has increased cell size | somatic clone phenotype, non-suppressible by Ets97D⁶¹³/Df(3R)ro80b

(Baltzer et al., 2009)

InR^UAS.cHa, Scer\GAL4^GMR.PF has visible phenotype, non-suppressible by ImpL2^MG2, Scer\GAL4^GMR.PF

(Honegger et al., 2008)

InR^UAS.cHa, Scer\GAL4^GMR.PB has visible phenotype, non-suppressible by Df(3L)AC1/Ilp2^UAS.cBa, Scer\GAL4^GMR.PB

(Brogiolo et al., 2001)

InR^UAS.cHa, Scer\GAL4^ey.PH has lethal | heat sensitive phenotype, non-suppressible by Tsc1^UAS.cPa, Scer\GAL4^ey.PH

(Potter et al., 2001)

InR^UAS.cHa, Scer\GAL4^ey.PH has lethal | heat sensitive phenotype, non-suppressible by gig^UAS.cTa, Scer\GAL4^ey.PH

(Potter et al., 2001)

InR^UAS.cHa, Scer\GAL4^ey.PH has visible phenotype, non-suppressible by Tsc1^UAS.cPa, Scer\GAL4^ey.PH

(Potter et al., 2001)

InR^UAS.cHa, Scer\GAL4^ey.PH has visible phenotype, non-suppressible by gig^UAS.cTa, Scer\GAL4^ey.PH

(Potter et al., 2001)

Enhancer of

Statement

Reference

InR^UAS.cHa, Scer\GAL4^GMR.PF is an enhancer of abnormal neuroanatomy phenotype of Scer\GAL4^GMR.PF, gogo^Scer\UAS.T1

(Mann et al., 2012)

InR^UAS.cHa, Scer\GAL4^GMR.PF is an enhancer of abnormal neuroanatomy phenotype of Scer\GAL4^GMR.PF, gogo^DDD.UAS

(Mann et al., 2012)

Phenotype Manifest In

Enhanced by

Statement

Reference

InR^UAS.cHa, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Pdk1^4ΔEP/Pdk1^EP3091, Scer\GAL4^GMR.PF

(Rintelen et al., 2001)

NOT Enhanced by

Statement

Reference

InR^UAS.cHa, Scer\GAL4^αTub84B.PP has fat body | somatic clone phenotype, non-enhanceable by Ets97D⁶¹³/Df(3R)ro80b

(Baltzer et al., 2009)

Suppressed by

Statement

Reference

InR^UAS.cHa, Scer\GAL4^αTub84B.PP has fat body | somatic clone phenotype, suppressible by srl^KG08646

(Tiefenböck et al., 2010)

InR^UAS.cHa, Scer\GAL4^αTub84B.PP has lipid droplet | somatic clone phenotype, suppressible by srl^KG08646

(Tiefenböck et al., 2010)

InR^UAS.cHa, Scer\GAL4^GMR.PF has eye phenotype, suppressible by ImpL2^EP5.66, Scer\GAL4^GMR.PF

(Honegger et al., 2008)

InR^UAS.cHa, Scer\GAL4^GMR.PF has eye phenotype, suppressible | partially by ImpL2^UAS.cHa, Scer\GAL4^GMR.PF

(Honegger et al., 2008)

InR^UAS.cHa, Scer\GAL4^GMR.PF has eye phenotype, suppressible by ImpL2^s.UAS, Scer\GAL4^GMR.PF

(Honegger et al., 2008)

InR^UAS.cHa, Scer\GAL4^GMR.PF has ommatidium phenotype, suppressible | partially by RpL8^RNAi.UAS, Scer\GAL4^GMR.PF

(Xu et al., 2008)

InR^UAS.cHa, Scer\GAL4^GMR.PF has ommatidium phenotype, suppressible | partially by Hsap\RPL8^DN.UAS, Scer\GAL4^GMR.PF

(Xu et al., 2008)

InR^UAS.cHa, Scer\GAL4^Act5C.PP has embryonic/larval fat body | somatic clone phenotype, suppressible | partially by hep^Act.UAS, Scer\GAL4^Act5C.PP

(Wang et al., 2005)

InR^UAS.cHa, Scer\GAL4^GMR.PF has eye phenotype, suppressible by B4^EP7-66

(Wittwer et al., 2005)

InR^UAS.cHa, Scer\GAL4^GMR.PB has eye phenotype, suppressible by Df(3L)AC1

(Brogiolo et al., 2001)

InR^UAS.cHa, Scer\GAL4^ey.PH has ommatidium phenotype, suppressible by gig^UAS.cTa/Tsc1^UAS.cPa, Scer\GAL4^ey.PH

(Potter et al., 2001)

InR^UAS.cHa, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Pk61C[+]/Pdk1^5ΔEP, Scer\GAL4^GMR.PF

(Rintelen et al., 2001)

InR^UAS.cHa, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Pdk1^4ΔEP/Pdk1^EP3091, Scer\GAL4^GMR.PF

(Rintelen et al., 2001)

InR^UAS.cHa, Scer\GAL4^ey.PH has eye phenotype, suppressible by Pten^UAS.cHa, Scer\GAL4^ey.PH

(Huang et al., 1999)

NOT suppressed by

Statement

Reference

InR^UAS.cHa, Scer\GAL4^αTub84B.PP has fat body | somatic clone phenotype, non-suppressible by Ets97D⁶¹³/Df(3R)ro80b

(Baltzer et al., 2009)

InR^UAS.cHa, Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by ImpL2^MG2, Scer\GAL4^GMR.PF

(Honegger et al., 2008)

InR^UAS.cHa, Scer\GAL4^GMR.PB has eye phenotype, non-suppressible by Df(3L)AC1/Ilp2^UAS.cBa, Scer\GAL4^GMR.PB

(Brogiolo et al., 2001)

InR^UAS.cHa, Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by Sps1^k11320

(Morey et al., 2001)

InR^UAS.cHa, Scer\GAL4^ey.PH has ommatidium phenotype, non-suppressible by Tsc1^UAS.cPa, Scer\GAL4^ey.PH

(Potter et al., 2001)

InR^UAS.cHa, Scer\GAL4^ey.PH has ommatidium phenotype, non-suppressible by gig^UAS.cTa, Scer\GAL4^ey.PH

(Potter et al., 2001)

Enhancer of

Statement

Reference

InR^UAS.cHa, Scer\GAL4^GMR.PF is an enhancer of photoreceptor cell R8 phenotype of Scer\GAL4^GMR.PF, gogo^Scer\UAS.T1

(Mann et al., 2012)

InR^UAS.cHa, Scer\GAL4^GMR.PF is an enhancer of medulla layer M1 phenotype of Scer\GAL4^GMR.PF, gogo^Scer\UAS.T1

(Mann et al., 2012)

InR^UAS.cHa, Scer\GAL4^GMR.PF is an enhancer of photoreceptor cell R8 phenotype of Scer\GAL4^GMR.PF, gogo^DDD.UAS

(Mann et al., 2012)

InR^UAS.cHa, Scer\GAL4^GMR.PF is an enhancer of medulla layer M1 phenotype of Scer\GAL4^GMR.PF, gogo^DDD.UAS

(Mann et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of InR^Scer\UAS.cHa strongly enhances the R8 photoreceptor phenotype seen when gogo^Scer\UAS.T1 is expressed under the control of Scer\GAL4^GMR.PF, resulting in an increase in the size of the blob-like structures seen in the medulla M1 layer. Premature axon stopping is also visible.

No R8 photoreceptor phenotypes are seen when gogo^FFD.Scer\UAS and InR^Scer\UAS.cHa are co-expressed under the control of Scer\GAL4^GMR.PF.

Expression of InR^Scer\UAS.cHa slightly enhances the R8 axon stopping phenotype seen when gogo^DDD.Scer\UAS is expressed under the control of Scer\GAL4^GMR.PF.

(Mann et al., 2012)

The effects of Scer\GAL4^αTub84B.PP>InR^Scer\UAS.cHa overexpression on cell growth and lipid droplet size in fat body cell clones are completely abrogated in a srl^KG08646 mutant genetic background.

(Tiefenböck et al., 2010)

Ets97D⁶¹³/Df(3R)ro80b does not significantly affect the increase in cell size seen in fat body clones expressing InR^Scer\UAS.cHa under the control of Scer\GAL4^αTub84B.PP.

(Baltzer et al., 2009)

ImpL2^EP5.66 suppresses the big eye phenotype resulting from the overexpression of InR^Scer\UAS.cHa driven by Scer\GAL4^GMR.PF.

ImpL2^MG2 fails to suppresses the big eye phenotype resulting from the overexpression of InR^Scer\UAS.cHa driven by Scer\GAL4^GMR.PF.

ImpL2^Scer\UAS.cHa partially suppresses the eye overgrowth resulting from the overexpression of InR^Scer\UAS.cHa driven by Scer\GAL4^GMR.PF.

ImpL2^s.Scer\UAS completely reverses the eye overgrowth resulting from the overexpression of InR^Scer\UAS.cHa driven by Scer\GAL4^GMR.PF.

(Honegger et al., 2008)

Expression of RpL8^{dsRNA.Scer\UAS} partially suppresses the increase in ommatidia size seen when InR^Scer\UAS.cHa is expressed under the control of Scer\GAL4^GMR.PF.

(Xu et al., 2008)

The distance between wing veins L3 and L4 is smaller than normal in flies co-expressing InR^Scer\UAS.cHa and Tctp^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ptc-559.1, resembling the phenotype seen in flies expressing Tctp^{dsRNA.Scer\UAS} alone under the control of Scer\GAL4^ptc-559.1.

(Hsu et al., 2007)

Overgrowth of cells in InR^Scer\UAS.cHa; Scer\GAL4^Act5C.PP clones in the larval fat body is largely suppressed by hep^Act.Scer\UAS.

(Wang et al., 2005)

The massive overgrowth of eyes seen in InR^Scer\UAS.cHa; Scer\GAL4^GMR.PF flies is suppressed by B4^EP7-66 or B4^Scer\UAS.cSa. Embryonic lethality due to InR^Scer\UAS.cHa; Scer\GAL4^en-e16E is also suppressed by B4^Scer\UAS.cSa.

(Wittwer et al., 2005)

The addition of Df(3L)AC1 to InR^Scer\UAS.cHa, Scer\GAL4^GMR.PB flies, suppresses the eye phenotype seen in these flies. The subsequent addition of Ilp2^Scer\UAS.cBa reverts the suppression.

(Brogiolo et al., 2001)

Coexpression of both gig^Scer\UAS.cTa and Tsc1^Scer\UAS.cPa (but not either gig^Scer\UAS.cTa or Tsc1^Scer\UAS.cPa alone) rescues the lethality and extra ommatidia phenotype of flies expressing InR^Scer\UAS.cHa under the control of Scer\GAL4^ey.PH.

(Potter et al., 2001)

Eye size in animals expressing InR^Scer\UAS.cHa under the control of Scer\GAL4^GMR.PF is restored almost to wild type by Pk61C^EP3091/Pk61C^4ΔEP, although eye roughness is increased.

(Rintelen et al., 2001)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\RPL8^DN.Scer\UAS partially suppresses the increase in ommatidia size seen when InR^Scer\UAS.cHa is expressed under the control of Scer\GAL4^GMR.PF.

(Xu et al., 2008)

Complementation and Rescue Data

Comments

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Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

InR^Scer\UAS.cHa

InR^UAS.cHa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Huang

(Huang et al., 1999)

Secondary FlyBase IDs

References (32)

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