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General Information
Symbol
Hsap\APP695.T.UAS
Species
H. sapiens
Name
FlyBase ID
FBal0102984
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Wild-type Hsap\APP695 is expressed under the control of UASt regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Flies expressing Hsap\APP695.T.Scer\UAS under the control of Scer\GAL4Mef2.PR eclose in normal numbers and have a normal lifespan. The flies show normal levels of climbing activity during the first two weeks of adulthood, but this declines during the subsequent weeks, so that by the end of week 4, the mutant flies show reduced climbing ability compared to controls. The mutant flies appear to lose their strength rather than their ability to show negative geotaxis, as they fall from the test cylinder. Similarly, 5 week old flies expressing Hsap\APP695.T.Scer\UAS under the control of Scer\GAL4Mef2.PR have reduced flying ability compared to controls.

The thoracic dorsal longitudinal flight muscle shows normal electrophysiological responses in 1- and 3-week old adults expressing Hsap\APP695.T.Scer\UAS under the control of Scer\GAL4Mef2.PR.

The indirect flight muscles of 3-week old adults expressing Hsap\APP695.T.Scer\UAS under the control of Scer\GAL4Mef2.PR appear normal.

Expression of Hsap\APP695.T.Scer\UAS under the control of Scer\GAL4GMR.PF has no obvious effect on eye morphology.

When Hsap\APP695.T.Scer\UAS is driven by Scer\GAL4Appl.G1a or Scer\GAL4179Y, an accumulation of organelles is seen in larval neurons. This phenotype is several fold more strong in males (about 200 clogs per 50μm) than in females (about 40 clogs per 50μm). Males also fail to develop to adulthood. When Hsap\APP695.T.Scer\UAS is driven by Scer\GAL4Appl.G1a, apoptosis is seen in the larval brain.

When expression is driven in the motoneurons muscles 6 and 7 (abdominal segment 3) total number of synaptic boutons is increased, percentage of satellite boutons is increased and the number of normal boutons is increased.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
Enhancer of
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

The progressive loss of climbing ability that is seen in flies expressing Hsap\APP695.T.Scer\UAS under the control of Scer\GAL4Mef2.PR is completely suppressed if they are also co-expressing Hsap\PARK2Scer\UAS.T:Hsap\MYC.

Expression of Hsap\APP695.T.Scer\UAS enhances the rough eye phenotype seen when par-1Scer\UAS.T:Hsap\MYC is expressed under the control of Scer\GAL4GMR.PF.

Expression of lkb1dsRNA.Scer\UAS.cWa suppresses the rough eye phenotype seen when par-1Scer\UAS.T:Hsap\MYC and Hsap\APP695.T.Scer\UAS are co-expressed under the control of Scer\GAL4GMR.PF.

Expression of Hsap\APP695.T.Scer\UAS enhances the rough eye phenotype seen when Hsap\MAPTScer\UAS.cWa is expressed under the control of Scer\GAL4GMR.PF.

Expression of Hsap\APP695.T.Scer\UAS enhances the rough eye phenotype seen when Hsap\MAPTR406W.Scer\UAS is expressed under the control of Scer\GAL4GMR.PF.

Expression of lkb1dsRNA.Scer\UAS.cWa enhances the rough eye phenotype seen when Hsap\APP695.T.Scer\UAS and Hsap\MAPTScer\UAS.cWa are co-expressed under the control of Scer\GAL4GMR.PF.

The organelle clogging phenotype seen in mutant larvae (Driven by Scer\GAL4Appl.G1a) is suppressed by the addition of Df(3L)GN24. The organelle clogging phenotype seen in mutant larvae (Driven by Scer\GAL4Appl.G1a) is suppressed by the addition of roblK/+. The apoptosis phenotype seen in Hsap\APP695.T.Scer\UAS, Scer\GAL4Appl.G1a animals is enhanced by the addition of Khc8 or Klc8ex94. The apoptosis phenotype seen in Hsap\APP695.T.Scer\UAS, Scer\GAL4Appl.G1a animals is enhanced by the addition of roblK or Df(3L)GN24.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Hsap\APP695.T.Scer\UAS
Hsap\APP695.T.UAS
Name Synonyms
Secondary FlyBase IDs
    References (4)