FlyBase Allele Report: Hsap\APP[695.T.UAS]

General Information

Symbol

Hsap\APP^695.T.UAS

Species

H. sapiens

Name

FlyBase ID

FBal0102984

Feature type

allele

Associated gene

Hsap\APP

Associated Insertion(s)

Carried in Construct

P{UAS-APP.695.T}

Key Links

Transgenic product class

wild_type

(Torroja et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Torroja et al., 1999)

Mutagen

in vitro construct

(Torroja et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-APP.695.T}

Cytology

Description

Wild-type Hsap\APP695 is expressed under the control of UASt regulatory sequences.

(Torroja et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Hsap\APP

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Alzheimer's disease

CEA

(Gunawardena and Goldstein, 2001)

model of inclusion body myositis

CEA

(Kim et al., 2011)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of Alzheimer's disease

is exacerbated by Klc^8ex94

(Gunawardena and Goldstein, 2001)

is exacerbated by Khc⁸

(Gunawardena and Goldstein, 2001)

is ameliorated by robl^K

(Gunawardena and Goldstein, 2001)

exacerbates neurodegenerative disease

modeled by Hsap\MAPT^R406W.UAS

(Wang et al., 2007)

model of neurodegenerative disease

is exacerbated by par-1^UAS.Tag:MYC

(Wang et al., 2007)

is exacerbated by Hsap\MAPT^R406W.UAS

(Wang et al., 2007)

model of inclusion body myositis

is ameliorated by Hsap\PRKN^UAS.Tag:MYC

(Kim et al., 2011)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

bouton | ectopic, with Scer\GAL4^unspecified

(Torroja et al., 1999)

larval nervous system, with Scer\GAL4^179Y

(Gunawardena and Goldstein, 2001)

larval nervous system, with Scer\GAL4^Appl.G1a

(Gunawardena and Goldstein, 2001)

Detailed Description

Statement

Reference

Flies expressing Hsap\APP^{695.T.Scer\UAS} under the control of Scer\GAL4^Mef2.PR eclose in normal numbers and have a normal lifespan. The flies show normal levels of climbing activity during the first two weeks of adulthood, but this declines during the subsequent weeks, so that by the end of week 4, the mutant flies show reduced climbing ability compared to controls. The mutant flies appear to lose their strength rather than their ability to show negative geotaxis, as they fall from the test cylinder. Similarly, 5 week old flies expressing Hsap\APP^{695.T.Scer\UAS} under the control of Scer\GAL4^Mef2.PR have reduced flying ability compared to controls.

The thoracic dorsal longitudinal flight muscle shows normal electrophysiological responses in 1- and 3-week old adults expressing Hsap\APP^{695.T.Scer\UAS} under the control of Scer\GAL4^Mef2.PR.

The indirect flight muscles of 3-week old adults expressing Hsap\APP^{695.T.Scer\UAS} under the control of Scer\GAL4^Mef2.PR appear normal.

(Kim et al., 2011)

Expression of Hsap\APP^{695.T.Scer\UAS} under the control of Scer\GAL4^GMR.PF has no obvious effect on eye morphology.

(Wang et al., 2007)

When Hsap\APP^{695.T.Scer\UAS} is driven by Scer\GAL4^Appl.G1a or Scer\GAL4^179Y, an accumulation of organelles is seen in larval neurons. This phenotype is several fold more strong in males (about 200 clogs per 50μm) than in females (about 40 clogs per 50μm). Males also fail to develop to adulthood. When Hsap\APP^{695.T.Scer\UAS} is driven by Scer\GAL4^Appl.G1a, apoptosis is seen in the larval brain.

(Gunawardena and Goldstein, 2001)

When expression is driven in the motoneurons muscles 6 and 7 (abdominal segment 3) total number of synaptic boutons is increased, percentage of satellite boutons is increased and the number of normal boutons is increased.

(Torroja et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Hsap\APP^695.T.UAS, Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF has visible | adult stage phenotype, enhanceable by Lkb1^RNAi.UAS.cWa, Scer\GAL4^GMR.PF

(Wang et al., 2007)

Hsap\APP^695.T.UAS, Scer\GAL4^Appl.G1a has increased cell death phenotype, enhanceable by Klc^8ex94

(Gunawardena and Goldstein, 2001)

Hsap\APP^695.T.UAS, Scer\GAL4^Appl.G1a has increased cell death phenotype, enhanceable by Khc⁸

(Gunawardena and Goldstein, 2001)

Suppressed by

Statement

Reference

Hsap\APP^695.T.UAS, Scer\GAL4^Mef2.PR has abnormal locomotor behavior | conditional phenotype, suppressible by Hsap\PRKN^UAS.Tag:MYC, Scer\GAL4^Mef2.PR

(Kim et al., 2011)

Hsap\APP^695.T.UAS, Scer\GAL4^GMR.PF, par-1^UAS.Tag:MYC has visible | adult stage phenotype, suppressible by Lkb1^RNAi.UAS.cWa, Scer\GAL4^GMR.PF

(Wang et al., 2007)

Hsap\APP^695.T.UAS, Scer\GAL4^Appl.G1a has increased cell death phenotype, suppressible by Df(3L)GN24

(Gunawardena and Goldstein, 2001)

Hsap\APP^695.T.UAS, Scer\GAL4^Appl.G1a has increased cell death phenotype, suppressible by robl^K

(Gunawardena and Goldstein, 2001)

Enhancer of

Statement

Reference

Hsap\APP^695.T.UAS, Scer\GAL4^GMR.PF is an enhancer of visible | adult stage phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^GMR.PF

(Wang et al., 2007)

Hsap\APP^695.T.UAS, Scer\GAL4^GMR.PF is an enhancer of visible | adult stage phenotype of Scer\GAL4^GMR.PF, par-1^UAS.Tag:MYC

(Wang et al., 2007)

Hsap\APP^695.T.UAS, Scer\GAL4^GMR.PF is an enhancer of visible | adult stage phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Wang et al., 2007)

Phenotype Manifest In

Enhanced by

Statement

Reference

Hsap\APP^695.T.UAS, Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Lkb1^RNAi.UAS.cWa, Scer\GAL4^GMR.PF

(Wang et al., 2007)

Suppressed by

Statement

Reference

Hsap\APP^695.T.UAS, Scer\GAL4^GMR.PF, par-1^UAS.Tag:MYC has eye phenotype, suppressible by Lkb1^RNAi.UAS.cWa, Scer\GAL4^GMR.PF

(Wang et al., 2007)

Hsap\APP^695.T.UAS, Scer\GAL4^Appl.G1a has larval nervous system phenotype, suppressible by Df(3L)GN24

(Gunawardena and Goldstein, 2001)

Hsap\APP^695.T.UAS, Scer\GAL4^Appl.G1a has larval nervous system phenotype, suppressible by robl^K

(Gunawardena and Goldstein, 2001)

Enhancer of

Statement

Reference

Hsap\APP^695.T.UAS, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^GMR.PF

(Wang et al., 2007)

Hsap\APP^695.T.UAS, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, par-1^UAS.Tag:MYC

(Wang et al., 2007)

Hsap\APP^695.T.UAS, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Wang et al., 2007)

Scer\GAL4^Appl.G1a/Hsap\APP^695.T.UAS is an enhancer of larval nervous system phenotype of Klc^8ex94

(Gunawardena and Goldstein, 2001)

Scer\GAL4^Appl.G1a/Hsap\APP^695.T.UAS is an enhancer of larval nervous system phenotype of Khc⁸

(Gunawardena and Goldstein, 2001)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The progressive loss of climbing ability that is seen in flies expressing Hsap\APP^{695.T.Scer\UAS} under the control of Scer\GAL4^Mef2.PR is completely suppressed if they are also co-expressing Hsap\PARK2^{Scer\UAS.T:Hsap\MYC}.

(Kim et al., 2011)

Expression of Hsap\APP^{695.T.Scer\UAS} enhances the rough eye phenotype seen when par-1^{Scer\UAS.T:Hsap\MYC} is expressed under the control of Scer\GAL4^GMR.PF.

Expression of lkb1^{dsRNA.Scer\UAS.cWa} suppresses the rough eye phenotype seen when par-1^{Scer\UAS.T:Hsap\MYC} and Hsap\APP^{695.T.Scer\UAS} are co-expressed under the control of Scer\GAL4^GMR.PF.

Expression of Hsap\APP^{695.T.Scer\UAS} enhances the rough eye phenotype seen when Hsap\MAPT^Scer\UAS.cWa is expressed under the control of Scer\GAL4^GMR.PF.

Expression of Hsap\APP^{695.T.Scer\UAS} enhances the rough eye phenotype seen when Hsap\MAPT^{R406W.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PF.

Expression of lkb1^{dsRNA.Scer\UAS.cWa} enhances the rough eye phenotype seen when Hsap\APP^{695.T.Scer\UAS} and Hsap\MAPT^Scer\UAS.cWa are co-expressed under the control of Scer\GAL4^GMR.PF.

(Wang et al., 2007)

The organelle clogging phenotype seen in mutant larvae (Driven by Scer\GAL4^Appl.G1a) is suppressed by the addition of Df(3L)GN24. The organelle clogging phenotype seen in mutant larvae (Driven by Scer\GAL4^Appl.G1a) is suppressed by the addition of robl^K/+. The apoptosis phenotype seen in Hsap\APP^{695.T.Scer\UAS}, Scer\GAL4^Appl.G1a animals is enhanced by the addition of Khc⁸ or Klc^8ex94. The apoptosis phenotype seen in Hsap\APP^{695.T.Scer\UAS}, Scer\GAL4^Appl.G1a animals is enhanced by the addition of robl^K or Df(3L)GN24.

(Gunawardena and Goldstein, 2001)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Hsap\APP^{695.T.Scer\UAS}

Hsap\APP^695.T.UAS

Name Synonyms

Secondary FlyBase IDs

References (4)

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