Allele Avic\GFP^E.3xP3

General Information
Symbol	Avic\GFP^E.3xP3	Species	A. victoria
Name		FlyBase ID	FBal0103092
Feature type	allele	Created / Updated	2006-05-31/2006-05-31
Associated gene	Avic\GFP

Allele class
Mutagen	in vitro construct \| regulatory fusion
Nature of the Allele
Allele class
Mutagen	in vitro construct \| regulatory fusion (Berghammer et al., 1999)
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Artificial promoter containing three binding sites for Mmus\Pax6 homodimers in front of a TATA box drives the expression of the enhanced variant of Avic\GFP (EGFP). (Berghammer et al., 1999) Three tandem repeats of an artificially derived Mmus\Pax6 homodimer binding site are fused upstream of a TATA box driving expression of "EGFP". (Horn et al., 2000)
Assay mode	In transgenic Drosophila (allele of foreign species in genome of drosophilid) (Berghammer et al., 1999, Horn and Wimmer, 2000) Whole-organism transient assay (allele of foreign species assayed in drosophilid) (Horn and Wimmer, 2000)
Carried in construct	M{3xP3-EGFP} (Lozovsky et al., 2002, Berghammer et al., 1999, Horn et al., 2000) Herm{3xP3-EGFP} (Berghammer et al., 1999, Horn et al., 2000) PBac{3xP3-EGFP} (Berghammer et al., 1999, Horn et al., 2000) P{3xP3-EGFP} (Horn et al., 2000) PBac{3xP3-EGFPafm} (Horn and Wimmer, 2000) PBac{3xP3-EGFPaf} (Horn and Wimmer, 2000) Herm{3xP3-EGFPaf} (Horn and Wimmer, 2000) M{3xP3-EGFPafm} (Horn and Wimmer, 2000) M{3xP3-EGFPaf} (Horn and Wimmer, 2000) P{GREENi} (Nagel et al., 2002) P{FlipG} (Nagel et al., 2002) Herm{y.W} (Wittkopp et al., 2002) Herm{Dvir\y.W} (Wittkopp et al., 2002) Mi{ET1} (Metaxakis et al., 2005) Mi{PR1} (Metaxakis et al., 2005) PBac{hs-Cctra.F1} (Pane et al., 2005) M{nos-int.NLS} (Bischof et al., 2007) M{vas-int.B} (Bischof et al., 2007) M{vas-int.NLS} (Bischof et al., 2007) M{nos-int.B} (Bischof et al., 2007)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1491 )
Bloomington	23802 y¹; Mi{ET1}MB02338 22683 w¹¹¹⁸ Mi{ET1}MB00043 22717 w¹¹¹⁸ Mi{ET1}MB00172 22778 w¹¹¹⁸ Mi{ET1}MB00368 22798 w¹¹¹⁸ Mi{ET1}MB00435 22803 w¹¹¹⁸ Mi{ET1}MB00450 22822 Mi{ET1}MB00522 w¹¹¹⁸ 22830 w¹¹¹⁸ Mi{ET1}MB00532 22863 w¹¹¹⁸ Mi{ET1}MB00659 22864 w¹¹¹⁸ Mi{ET1}MB00664 22944 w¹¹¹⁸ Mi{ET1}MB00961 22955 w¹¹¹⁸ Mi{ET1}MB00990
	More stocks available...
Notes on Origin
Discoverer

Comments
	Carried in plasmids pM{3xP3-EGFPaf-OriKan}, pHerm{3xP3-EGFPaf-OriKan} or pPBac{3xP3-EGFPaf-OriKan}, injected into D.melanogaster or D.virilis embryos and used as a donor plasmid in a plasmid-based transposition assay. (Horn and Wimmer, 2000)
Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Avic\GFP^E.3xP3
Name Synonym
Secondary FlyBase IDs

References ( 11 )
Research paper	Bischof et al., 2007, Proc. Natl. Acad. Sci. USA 104(9): 3312--3317 An optimized transgenesis system for Drosophila using germ-line-specific φC31 integrases. [FBrf0192974] Metaxakis et al., 2005, Genetics 171(2): 571--581 Minos as a genetic and genomic tool in Drosophila melanogaster. [FBrf0190750] Pane et al., 2005, Genetics 171(2): 615--624 Evolutionary conservation of Ceratitis capitata transformer gene function. [FBrf0190755] Lozovsky et al., 2002, Genetics 160(2): 527--535 Unexpected Stability of mariner Transgenes in Drosophila. [FBrf0144896] Nagel et al., 2002, Dev. Genes Evol. 212(2): 93--98 Green fluorescent protein as a convenient and versatile marker for studies on functional genomics in Drosophila. [FBrf0146975] Wittkopp et al., 2002, Curr. Biol. 12(18): 1547--1556 Evolution of yellow gene regulation and pigmentation in Drosophila. [FBrf0151844] Horn et al., 2000, Dev. Genes Evol. 210(12): 623--629 Highly sensitive, fluorescent transformation marker for Drosophila transgenesis. [FBrf0132292] Horn and Wimmer, 2000, Dev. Genes Evol. 210(12): 630--637 A versatile vector set for animal transgenesis. [FBrf0134553] Berghammer et al., 1999, Nature 402(6760): 370--371 A universal marker for transgenic insects. [FBrf0122963]
Supplementary material	Bischof et al., 2006, Proc. Natl. Acad. Sci. USA 104(9): SI Text. [FBrf0198668]
Personal communication to FlyBase	Bischof and Basler, 2007.11.7, Information on M{vas-int.Dm} stocks. Information on M{vas-int.Dm} stocks. [FBrf0200341]

Allele Avic\GFPE.3xP3

Allele Avic\GFP^E.3xP3