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General Information
Symbol
Dmel\Applsd.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0103093
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-APPLSD, APPLsd
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Deletion of the cleavage site (therefore protein tethered to membrane), transmembrane domain, Gobinding domain, internalization signal of the Appl coding region.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expressing Applsd.UAS under the control of Scer\GAL4GMR91H05 results in adult giant fibers presenting morphological and functional defects: there is a severe decrease in the frequency of synaptic response upon high frequency stimulation (10 trains of 10 stimulations at 100 Hz); although 2-3 days old adults present terminals in the synaptic target region (second thoracic neuromere) and without pruning defects, most terminals are stunted or exhibit bulb-like endings; in 14 and 28 days old adults, terminals are progressively seen outside the target area (including second thoracic neuromere, CvC and brain).

Overexpression of Applsd.Scer\UAS for 48 hours, under the control of RU-inducible Scer\GAL4Mef2.247.Switch, does not affect short term memory.

Expression of Applsd.Scer\UAS driven by Scer\GAL4Appl.PU induces significant cell death in the ventral nerve cord compared to controls.

28 day old flies expressing Applsd.Scer\UAS under the control of Scer\GAL4GMR.PU show degeneration of the lamina cortex.

Flies expressing Applsd.Scer\UAS under the control of Scer\GAL4Appl.G1a show increased vacuolisation in the brain compared to controls. 55% of 5 day old flies contain single vacuoles that are confined to the lamina.

Expression of Applsd.Scer\UAS under the control of Scer\GAL4Bx-MS1096 results in a reduction in wing size and breaks in the wing vein pattern. The wing discs of these animals show a large increase in cell death throughout the wing pouch.

Expression of Applsd.Scer\UAS pre-synaptically (under the control of Scer\GAL4C164) results in a dramatic increase in bouton number.

When Applsd.Scer\UAS is driven by Scer\GAL4sca-537.4 a strong bristle transformation phenotype is seen.

When expression is driven in the motoneurons extra small boutons of abnormal morphology develop. Many small satellite boutons bud off from a central bouton of normal appearance. Molecular markers suggest that the extra boutons are sufficiently normal to form functional synapses. When expression is driven in the motoneurons muscles 6 and 7 (abdominal segment 3) total number of synaptic boutons is increased, percentage of satellite boutons is increased and the number of normal boutons is increased. When Applsd.Scer\UAS expression is driven in the motoneurons muscles 6 and 7 (abdominal segment 3) in combination with Appld total number of synaptic boutons is increased, percentage of satellite boutons is increased and the number of normal boutons is increased.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Expression of Applsd.Scer\UAS under the control of Scer\GAL4Appl.G1a significantly enhances the increase in vacuolisation seen in the brains of SNF4Aγloe mutant third instar larvae.

The wing phenotypes caused by expression of Applsd.Scer\UAS under the control of Scer\GAL4Bx-MS1096 are suppressed by co-expression of APP-BP1Scer\UAS.cKa.

The increased cell death seen in the wing discs of animals expressing Applsd.Scer\UAS under the control of Scer\GAL4Bx-MS1096 is suppressed by co-expression of APP-BP1Scer\UAS.cKa, and is enhanced by APP-BP1Ex62/+.

A Fas2e76 background suppresses the increase in bouton number observed when Applsd.Scer\UAS is overexpressed pre-synaptically (under the control of Scer\GAL4C164).

Overexpression of both Applsd.Scer\UAS and Fas2Scer\UAS.cAa both pre- and post-synaptically (under the control of Scer\GAL4C380 and Scer\GAL4BG487) increase the number of boutons by >400%.

The organelle clogging phenotype seen in mutant larvae (Driven by Scer\GAL4Appl.G1a) is suppressed by the addition of roblK/+.

When Applsd.Scer\UAS expression is driven in the motoneurons muscles 6 and 7 (abdominal segment 3) in combination with eag1, Sh14 total number of synaptic boutons is decreased compared to Applsd.Scer\UAS alone, percentage of satellite boutons is decreased compared to Applsd.Scer\UAS alone and the number of normal boutons is increased compared to Applsd.Scer\UAS alone.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of Applsd.Scer\UAS in RU-fed flies suppresses the short-term memory defect found in Appld/+; Scer\GAL4Mef2.247.Switch flies. In contrast, in the absence of RU-feeding, Appld/+; Scer\GAL4Mef2.247.Switch/Applsd.Scer\UAS flies exhibit scores that are indistinguishable from controls and significantly lower than that found in wild-type flies.

RU-induced expression of Applsd.Scer\UAS under the control of Scer\GAL4Mef2.247.Switch fails to rescue the long term memory deficit caused by Appld/+ partial loss-of-function.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Applsd.Scer\UAS
Applsd.UAS
Name Synonyms
Secondary FlyBase IDs
    References (10)