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General Information
Symbol
Dmel\shgUAS.sgGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0103570
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-DEFL, UAS-E-Cadherin-GFP, UAS-DE-cad-GFP, UAS-E-Cad-GFP, UAS-DE-Cadherin:GFP, UAS-de-cadherin-GFP, DE-Cadherin-GFP, e-cad-GFP
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UASt regulatory sequences drive expression of a full-length shg cDNA. The open reading frame is tagged with sgGFP fluorescent protein.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Overexpression of shgScer\UAS.T:Avic\GFP-rs in the embryonic epidermis under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 results in delayed wound closure compared with wild-type.
The expression of shgScer\UAS.T:Avic\GFP-rs alone under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 has no obvious effect on the germline stem cell population in adult testes, as compared to controls.
shgScer\UAS.T:Avic\GFP-rs driven by Scer\GAL4nos.PU does not affect average germline stem cell number in third instar larval testes.
Male germline stem cell clones expressing shgScer\UAS.T:Avic\GFP-rs under the control of Scer\GAL4nos.PS show a considerably higher frequency of symmetric self renewal compared to controls.
Expression of shgScer\UAS.T:Avic\GFP-rs under the control of Scer\GAL4AB1 causes a bulging phenotype on each cell of the larval salivary gland, but with a normal size of the lumen.
Expression of shgScer\UAS.T:Avic\GFP-rs under the control of Scer\GAL4GMR.PF results in low level failure to switch cone-cell contacts.
Expression of shgScer\UAS.T:Avic\GFP-rs under the control of Scer\GAL4btl.PS delays the elongation of the dorsal branches. The tube remains multicellular in the late stage of elongation rather than becoming unicellular, but tracheal development is eventually completed.
Embryos expressing shgScer\UAS.T:Avic\GFP-rs under the control of Scer\GAL4fkh.PH form normal salivary glands.
Clones of shgScer\UAS.T:Avic\GFP-rs; Scer\GAL4Act5C.PI cells in the pupal retina (at 40 hours after puparium formation) have little effect on the morphology of cone cells or primary pigment cells. However, when anterior and posterior cone cells are shgScer\UAS.T:Avic\GFP-rs; Scer\GAL4Act5C.PI, an interface sometimes forms between these cone cells instead of between dorsal and ventral cones cells as in wild-type.
When transiently transformed with Scer\GAL4Act5C.PHb into S2 cells, promoted a strong cell aggregation phenotype.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Enhancer of
Suppressor of
Statement
Reference
shgUAS.sgGFP/Scer\GAL4upd1.PU is a suppressor of cell-cell adherens junction & hub cell phenotype of PDZ-GEF3/PDZ-GEF4
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference
Co-expression of shgScer\UAS.T:Avic\GFP-rs under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 does not suppress the decreased germline stem cell numbers present in the testis of adults expressing either MtorGD13871 or MtorHMS00735 under the control of Scer\GAL4nos.UTR.T:Hsim\VP16.
shgScer\UAS.T:Avic\GFP-rs driven by Scer\GAL4nos.PU suppresses the reduced number of germline stem cells seen in lid10424/Df(2L)BSC184 third instar larval testes.
Expression of shgScer\UAS.T:Avic\GFP-rs in germ cells under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 does not suppress the germline stem cell (GSC) loss seen in chic11/chic01320 mutant testes. Expression of shgScer\UAS.T:Avic\GFP-rs in germ cells under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 does not suppress the germline stem cell (GSC) loss seen in chic221/chic01320 mutant testes.
Expression of shgScer\UAS.T:Avic\GFP-rs in germline stem cells (GSCs) under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 does not suppress the GSC loss seen in His2Av810 mutant testes.
Co-expression of shgScer\UAS.T:Avic\GFP-rs with PakScer\UAS.T:Myr1 under the control of Scer\GAL4AB1 completely suppresses the bulging phenotype, and partially suppresses the PakScer\UAS.T:Myr1 salivary gland lumen expansion phenotype.
Co-expression of shgScer\UAS.T:Avic\GFP-rs does not significantly change the impaired epidermal cell elongation phenotype seen during dorsal closure stages in embryos expressing Rab11S25N.Scer\UAS under the control of Scer\GAL4en-e16E.
Expression of shgScer\UAS.T:Avic\GFP-rs under the control of Scer\GAL4hs.PU partially rescues the formation of egg chambers in Inx2FA42 females.
Ectopic expression of shgScer\UAS.T:Avic\GFP-rs strongly suppresses the Scer\GAL4GMR.PF>cindrdsRNA.PC.PD.Scer\UAS mispatterning phenotype and reduces the distance between interommatidial precursor cells.
Expression of shgScer\UAS.T:Avic\GFP-rs strongly enhances the cone cell switching phenotype seen 41-42 hours after pupal formation when pyd3.dsRNA.Scer\UAS is expressed under the control of Scer\GAL4GMR.PF. A greater proportion of ommatidia maintain the immature A/P configuration rather than switching to the E/P configuration as is seen in controls.
Expression of shgScer\UAS.T:Avic\GFP-rs partially suppresses the tracheal defects seen when Src42ACA.Scer\UAS is expressed under the control of Scer\GAL4btl.PS. Tracheal cells maintain apico-basal polarity for at least two hours longer than when Src42ACA.Scer\UAS is expressed alone, and dorsal branch extension is also partially restored.
The salivary gland defects seen in embryos expressing Rac1V12.Scer\UAS under the control of Scer\GAL4fkh.PH are partially suppressed by co-expression of shgScer\UAS.T:Avic\GFP-rs.
Expression of shgScer\UAS.T:Avic\GFP-rs driven by Scer\GAL4da.PU almost fully suppresses the supernumerary spermathecae phenotype (as well as wing and eye phenotypes) in PDZ-GEF4/PDZ-GEF3 flies.
shgScer\UAS.T:Avic\GFP-rs; Scer\GAL4upd rescues the formation of adherens junctions between male germline stem cells and hub cells in the germinal proliferation center of Gef264/Gef263.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
The defects in the left-right asymmetry of the hindgut which are seen in homozygous shgR758 embryos are rescued by expression of shgScer\UAS.T:Avic\GFP-rs under the control of either Scer\GAL4mat.αTub67C.T:Hsim\VP16 or Scer\GAL4455.2, but are not rescued by expression of shgScer\UAS.T:Avic\GFP-rs under the control of either Scer\GAL448Y or Scer\GAL4how-24B.
The addition of shgScer\UAS.T:Avic\GFP-rs, driven by Scer\GAL4btl.PS, into shg2/Df(2R)E2 flies rescues their tracheal fusion phenotype.
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments
Carried in a plasmid and co transfected with Scer\GAL4Act5C.PHb into S2 cells.
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
shgScer\UAS.T:Avic\GFP-rs
shgUAS.rsGFP
shgUAS.sgGFP
Name Synonyms
Secondary FlyBase IDs
    References (37)