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General Information
Symbol
Dmel\scribdt6
Species
D. melanogaster
Name
FlyBase ID
FBal0103573
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
dt6
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Like wild type, cells from scribdt6/scrib673 transheterozygous wing discs are arranged in epithelial monolayers and maintain the folded structure of the tissue. However, these mutant scrib cells show cuboidal rather than columnar morphology.

scribdt6/scrib673 transheterozygous wing discs show an intermediate increase in both the surface area and volume compared to wild type and contain approximately 300% more cells than wild type.

Adult eye tissue homozygous for scribdt6 is readily obtained, although it is rough, enlarged and folded.

scribdt6 germ line clone embryos display a consistent failure of dorsal closure and mildly penetrant anterior open defects.

Adherens junction formation is normal but septate junctions are severely disrupted in scribdt6/scrib673 transheterozygous wing discs.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of scribΔLRR.Scer\UAS.P\T.T:Avic\GFP under the control of Scer\GAL469B fails to rescue the cell polarization and disc overgrowth phenotypes of scrib673/scribdt6 transheterozygotes.

Expression of scribΔLRR.Scer\UAS.P\T.T:Myr6,T:Avic\GFP under the control of Scer\GAL469B fails to rescue the cell polarization and disc overgrowth phenotypes of scrib673/scribdt6 transheterozygotes.

Expression of scrib4.Scer\UAS.P\T.T:Avic\GFP or scribΔPDZs.Scer\UAS.P\T.T:Avic\GFP under the control of Scer\GAL469B provides a more complete rescue of the size phenotype of scrib673/scribdt6 transheterozygous discs compared to scrib null discs. Increasing the level of transgene expression via raising larvae at 29 oC further increases this rescue. Moreover, the apicolateral enhancement of septate junction markers is restored and cell shape is columnar rather than cuboidal in rescued scrib673/scribdt6 discs.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer

The reason for the early lethality of scribdt6/scribdt12 and scribdt6/Df(3R)Tl-X transheterozygotes is not known but may be because scribdt6 and scribdt12 were generated on a similar parental chromosome and share an accessory lethal.

Comments
Comments

Heteroallelic combinations between scribdt6, scribdt12 and scrib882 suggest that scribdt6 is the most severe mutation of these three.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (3)