FB2025_01 , released February 20, 2025
Allele: Dmel\scrib673
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General Information
Symbol
Dmel\scrib673
Species
D. melanogaster
Name
FlyBase ID
FBal0103576
Feature type
allele
Associated gene
Dmel\scrib
Associated Insertion(s)
Carried in Construct
Also Known As
scrib2, l(3)673
Key Links
Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Zeitler et al., 2004, Bilder and Perrimon, 2000)
Mutagen
ethyl methanesulfonate
(Zeitler et al., 2004, Bilder and Perrimon, 2000)
Progenitor genotype
Cytology
Description
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Modifiers Based on Experimental Evidence ( 1 )
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class

      abnormal cell polarity | cell autonomous | larval stage | somatic clone

      (Huang et al., 2023)

      abnormal cell polarity | larval stage (with scrib5)

      (Huang et al., 2023)

      abnormal cell polarity | larval stage (with scribird15)

      (Zeitler et al., 2004)

      abnormal cell polarity | larval stage | recessive

      (Zeitler et al., 2004)

      abnormal cell shape | larval stage (with scribdt6)

      (Zeitler et al., 2004)

      abnormal cell shape | larval stage (with scribdt12)

      (Zeitler et al., 2004)

      abnormal cell shape | larval stage (with scribird15)

      (Zeitler et al., 2004)

      abnormal cell shape | larval stage | recessive

      (Zeitler et al., 2004)

      abnormal developmental rate | third instar larval stage (with Df(3R)Tl-X)

      (Zeitler et al., 2004)

      abnormal developmental rate | third instar larval stage (with scribird15)

      (Zeitler et al., 2004)

      abnormal mitotic cell cycle | somatic clone

      (Brumby and Richardson, 2003)

      abnormal neuroanatomy | larval stage

      (Roche et al., 2002)

      abnormal neuroanatomy | larval stage (with Df(3R)Tl-X)

      (Roche et al., 2002)

      abnormal neurophysiology | larval stage

      (Roche et al., 2002)

      abnormal neurophysiology | larval stage (with Df(3R)Tl-X)

      (Roche et al., 2002)

      abnormal size | larval stage (with scrib1)

      (Zeitler et al., 2004)

      abnormal size | larval stage (with scrib882)

      (Zeitler et al., 2004)

      abnormal size | larval stage (with scribdt6)

      (Zeitler et al., 2004)

      abnormal size | larval stage (with scribdt12)

      (Zeitler et al., 2004)

      abnormal size | larval stage (with scribdt14)

      (Zeitler et al., 2004)

      abnormal size | larval stage (with scribird15)

      (Zeitler et al., 2004)

      abnormal size | larval stage | recessive

      (Zeitler et al., 2004)

      abnormal smell perception | adult stage (with scribsmi97B)

      (Ganguly et al., 2003)

      increased body size | larval stage | somatic clone

      (Menut et al., 2007)

      increased body size | third instar larval stage (with Df(3R)Tl-X)

      (Zeitler et al., 2004)

      increased body size | third instar larval stage (with scrib1)

      (Zeitler et al., 2004)

      increased body size | third instar larval stage (with scribird15)

      (Zeitler et al., 2004)

      increased cell number | larval stage (with scribdt6)

      (Zeitler et al., 2004)

      increased cell number | larval stage (with scribird15)

      (Zeitler et al., 2004)

      lethal | recessive

      (Kidd et al., 1999)

      lethal - all die before end of larval stage (with Df(3R)Tl-X)

      (Zeitler et al., 2004)

      lethal - all die before end of larval stage (with scrib1)

      (Zeitler et al., 2004)

      lethal - all die before end of larval stage (with scribird15)

      (Zeitler et al., 2004)

      lethal - all die before end of P-stage | somatic clone

      (Jiang et al., 2011, Menut et al., 2007, Zeitler et al., 2004)

      lethal - all die before end of pupal stage (with scrib882)

      (Zeitler et al., 2004)

      lethal - all die before end of pupal stage (with scribdt6)

      (Zeitler et al., 2004)

      lethal - all die before end of pupal stage (with scribdt12)

      (Zeitler et al., 2004)

      lethal - all die before end of pupal stage (with scribdt14)

      (Zeitler et al., 2004)

      neoplasia | larval stage, with Scer\GAL4ptc-559.1, scribHMS01490

      (Huang et al., 2023)

      neoplasia | somatic clone

      (Khan et al., 2013, Uhlirova et al., 2005)

      some die during pharate adult stage | somatic clone

      (Menut et al., 2007, Zeitler et al., 2004)

      some die during P-stage | somatic clone

      (Jiang et al., 2011)

      some die during pupal stage (with scrib882)

      (Zeitler et al., 2004)

      some die during pupal stage (with scribdt6)

      (Zeitler et al., 2004)

      some die during pupal stage (with scribdt12)

      (Zeitler et al., 2004)

      some die during pupal stage (with scribdt14)

      (Zeitler et al., 2004)

      some die during third instar larval stage (with Df(3R)Tl-X)

      (Zeitler et al., 2004)

      some die during third instar larval stage (with scrib1)

      (Zeitler et al., 2004)

      some die during third instar larval stage (with scribird15)

      (Zeitler et al., 2004)
      Phenotype Manifest In

      adherens junction

      (Zeitler et al., 2004)

      adherens junction (with scrib1)

      (Zeitler et al., 2004)

      adult head | somatic clone

      (Zeitler et al., 2004)

      epithelial cell

      (Zeitler et al., 2004)

      epithelial cell (with scribdt6)

      (Zeitler et al., 2004)

      epithelial cell (with scribdt12)

      (Zeitler et al., 2004)

      epithelial cell (with scribird15)

      (Zeitler et al., 2004)

      eye | somatic clone

      (Jiang et al., 2011, Uhlirova et al., 2005)

      eye-antennal disc | somatic clone

      (Jiang et al., 2011)

      eye disc | somatic clone

      (Khan et al., 2013, Menut et al., 2007)

      ommatidial precursor cluster

      (Zeitler et al., 2004)

      septate junction

      (Zeitler et al., 2004)

      septate junction (with scrib1)

      (Zeitler et al., 2004)

      septate junction (with scribdt6)

      (Zeitler et al., 2004)

      synaptic vesicle | larval stage

      (Roche et al., 2002)

      synaptic vesicle | larval stage (with Df(3R)Tl-X)

      (Roche et al., 2002)

      type I bouton | larval stage

      (Roche et al., 2002)

      type I bouton | larval stage (with Df(3R)Tl-X)

      (Roche et al., 2002)

      wing disc

      (Zeitler et al., 2004)

      wing disc (with scrib1)

      (Zeitler et al., 2004)

      wing disc (with scrib882)

      (Zeitler et al., 2004)

      wing disc (with scribdt6)

      (Zeitler et al., 2004)

      wing disc (with scribdt12)

      (Zeitler et al., 2004)

      wing disc (with scribdt14)

      (Zeitler et al., 2004)

      wing disc (with scribird15)

      (Zeitler et al., 2004)

      wing disc | cell autonomous | larval stage | somatic clone

      (Huang et al., 2023)

      wing disc | larval stage, with Scer\GAL4ptc-559.1, scribHMS01490

      (Huang et al., 2023)

      wing disc | larval stage (with scrib5)

      (Huang et al., 2023)
      Detailed Description
      Statement
      Reference

      scrib673 mutant eye disc clones are neoplastically transformed.

      (Khan et al., 2013)

      Homozygous clones in the eye-antennal disc results in defects in disc morphology and differentiation. Most of these animals die as pupae, and the few adult escapers are eyeless.

      (Jiang et al., 2011)

      Stage 11 embryos lacking both maternal and zygotic scrib673 exhibit lost or severely disrupted tubule cell polarity.

      (Campbell et al., 2009)

      Mutant eye discs (generated using the eyFLP-cell lethal system) show disorganised cellular architecture and reduced differentiation compared to wild type.

      Animals containing mutant eye discs generated using the eyFLP-cell lethal system die as pharate adults without heads. Larvae containing these discs are increased in size compared to control larvae when examined as pupariation commences.

      (Menut et al., 2007)

      Cells from scrib673 wing discs are round (rather than elongated as in wild type) and have a multilayered organisation (rather than monolayered as in wild type), forming a solid spherical mass rather than the flat disc seen in wild type animals.

      scrib673 wing discs show a dramatic increase in both the surface area and volume compared to wild type.

      Animals with eyes homozygous for scrib673 never eclose, instead dying as headless pharate adults. The eye discs of these animals show disorganised ommatidial preclusters.

      Adherens junctions are mislocalized and septate junctions are absent in scrib673 wing discs.

      Cells from scribird15/scrib673 transheterozygous wing discs are round (rather than elongated as in wild type) and have a multilayered organisation (rather than monolayered as in wild type), forming a solid spherical mass rather than the flat disc seen in wild type animals.

      scribird15/scrib673 transheterozygous wing discs show a dramatic increase in both the surface area and volume compared to wild type and contain approximately 500% more cells than wild type.

      Like wild type, cells from scrib882/scrib673 transheterozygous wing discs are arranged in epithelial monolayers and maintain the folded structure of the tissue.

      scrib882/scrib673 transheterozygous wing discs show a very slight increase in both the surface area and volume compared to wild type.

      Like wild type, cells from scribdt6/scrib673 transheterozygous wing discs are arranged in epithelial monolayers and maintain the folded structure of the tissue. However, these mutant scrib cells show cuboidal rather than columnar morphology.

      scribdt6/scrib673 transheterozygous wing discs show an intermediate increase in both the surface area and volume compared to wild type and contain approximately 300% more cells than wild type.

      Like wild type, cells from scribdt12/scrib673 transheterozygous wing discs are arranged in epithelial monolayers and maintain the folded structure of the tissue. However, these scribdt12 mutant cells display altered shapes.

      scribdt12/scrib673 transheterozygous wing discs show an intermediate increase in both the surface area and volume compared to wild type.

      Like wild type, cells from scribdt14/scrib673 transheterozygous wing discs are arranged in epithelial monolayers and maintain the folded structure of the tissue.

      scribdt14/scrib673 transheterozygous wing discs show a very slight increase in both the surface area and volume compared to wild type.

      scrib1/scrib673 transheterozygous wing discs show a dramatic increase in both the surface area and volume compared to wild type.

      Adherens junction formation is normal but septate junctions are severely disrupted in scribdt6/scrib673 transheterozygous wing discs.

      Adherens junctions are mislocalized and septate junctions are absent in scrib1/scrib673 transheterozygous wing discs.

      (Zeitler et al., 2004)

      When somatic clones are made in the eye disc, ectopic DNA replication and mitoses are seen.

      (Brumby and Richardson, 2003)

      scribsmi97B/scrib673 adults show a significantly lower avoidance response to benzaldehyde compared to controls.

      (Ganguly et al., 2003)

      Embryos derived from scrib673 homozygous germline clones and lacking both maternal and zygotic scrib function have a poorly differentiated and convoluted cuticle.

      (Tanentzapf and Tepass, 2003)

      scrib673 and scrib673/Df(3R)Tl-X third instar larvae exhibit a decrease in synaptic bouton size and a statistically significant increase in both vesicle density and basal lamina thickness. They also exhibit a decrease in the number of active zones per cross-sectional area, although this is statistically significant only in scrib673 homozygous boutons. The distribution and density of reserve vesicles (RP) appears to be specifically affected in scrib673/Df(3R)Tl-X mutants, as the number of vesicles up to 200nm around the active zone, which is assumed to encompass the readily-releasable vesicle pool (RRP). scrib673/Df(3R)Tl-X third instar larvae exhibit a significant decrease in the frequency of miniature synaptic events. In addition these larvae exhibit a slight but significant decrease in the amplitude of miniature synaptic events. High frequency stimulation causes scrib673/Df(3R)Tl-X synapses to exhibit both a lack of facilitation at low Ca2+ concentrations and faster synaptic depression at higher Ca2+ concentrations. scrib673/Df(3R)Tl-X mutants exhibit a modest potentiation after a train of high-frequency stimulation (post-tetanic potentiation), in contrast to the amplitude of wild-type excitatory junctional currents, which is potentiated more than twofold after post-tetanic potentiation.

      (Roche et al., 2002)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Statement
      Reference

      Egfr2.A887T.UAS, Scer\GAL4Act.PU, scrib673 has neoplasia | somatic clone | larval stage phenotype, suppressible by psidin55D4/psidin55D4

      (Sheng and Du, 2020)

      Egfr2.A887T.UAS, Scer\GAL4Act.PU, scrib673 has neoplasia | somatic clone | larval stage phenotype, suppressible by psidinS678D.UAS.Tag:HA, psidin55D4/psidin55D4/Scer\GAL4Act.PU

      (Sheng and Du, 2020)

      Egfr2.A887T.UAS, Scer\GAL4Act.PU, scrib673 has increased occurrence of cell division | somatic clone | larval stage phenotype, suppressible by psidin55D4/psidin55D4

      (Sheng and Du, 2020)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has neoplasia | somatic clone | third instar larval stage phenotype, suppressible | partially | cell autonomous | cell non-autonomous | somatic clone by Atg13Δ81/Atg13Δ81

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has neoplasia | somatic clone | third instar larval stage phenotype, suppressible by Atg13+tCH322-168O18, Atg13Δ81/Atg13Δ81

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has neoplasia | somatic clone | third instar larval stage phenotype, suppressible | cell non-autonomous | partially by Atg14Δ5.2/Atg14Δ5.2

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has neoplasia | somatic clone | third instar larval stage phenotype, suppressible | partially | somatic clone by Atg13Δ81/Atg13Δ81/Atg14Δ5.2/Atg14Δ5.2

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has neoplasia | somatic clone | third instar larval stage phenotype, suppressible | partially by Atg13ey.3.5, Atg13Δ81/Atg13Δ81

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has abnormal size | somatic clone | third instar larval stage phenotype, suppressible | partially | cell autonomous | cell non-autonomous | somatic clone by Atg13Δ81/Atg13Δ81

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has abnormal size | somatic clone | third instar larval stage phenotype, suppressible by Atg13+tCH322-168O18, Atg13Δ81/Atg13Δ81

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has abnormal size | somatic clone | third instar larval stage phenotype, suppressible | cell non-autonomous | partially by Atg14Δ5.2/Atg14Δ5.2

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has abnormal size | somatic clone | third instar larval stage phenotype, suppressible | partially | somatic clone by Atg13Δ81/Atg13Δ81/Atg14Δ5.2/Atg14Δ5.2

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has abnormal size | somatic clone | third instar larval stage phenotype, suppressible | partially by Atg13ey.3.5, Atg13Δ81/Atg13Δ81

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has increased cell number | somatic clone | third instar larval stage phenotype, suppressible | cell non-autonomous by Atg13Δ81/Atg13Δ81

      (Katheder et al., 2017)

      Atg13Δ81, Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has neoplasia | somatic clone | third instar larval stage phenotype, suppressible by Atg14EY14568/Atg14Δ5.2

      (Katheder et al., 2017)

      Atg13Δ81, Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has neoplasia | somatic clone | third instar larval stage phenotype, suppressible by Atg8ad4/Atg8ad4

      (Katheder et al., 2017)

      Atg13Δ81, Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has abnormal size | somatic clone | third instar larval stage phenotype, suppressible by Atg14EY14568/Atg14Δ5.2

      (Katheder et al., 2017)

      Atg13Δ81, Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has abnormal size | somatic clone | third instar larval stage phenotype, suppressible by Atg8ad4/Atg8ad4

      (Katheder et al., 2017)

      scrib673 has neoplasia | somatic clone phenotype, suppressible by Scer\GAL4Tub.PU/hthHMS01112

      (Khan et al., 2013)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has neoplasia | somatic clone phenotype, suppressible | somatic clone by sds22UAS.cJa, Scer\GAL4Act.PU

      (Jiang et al., 2011)

      scrib673 has neoplasia | somatic clone phenotype, suppressible by Scer\GAL4Act5C.PI/hepAct.UAS

      (Uhlirova et al., 2005)
      NOT suppressed by
      Statement
      Reference

      Egfr2.A887T.UAS, Scer\GAL4Act.PU, scrib673 has neoplasia | somatic clone | larval stage phenotype, non-suppressible by psidinS678A.UAS.Tag:HA, psidin55D4/psidin55D4/Scer\GAL4Act.PU

      (Sheng and Du, 2020)

      Egfr2.A887T.UAS, Scer\GAL4Act.PU, scrib673 has neoplasia | somatic clone | larval stage phenotype, non-suppressible by psidinUAS.cKa, psidin55D4/psidin55D4/Scer\GAL4Act.PU

      (Sheng and Du, 2020)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has neoplasia | somatic clone | third instar larval stage phenotype, non-suppressible | partially | cell autonomous | cell non-autonomous | somatic clone by Atg13Δ81/Atg13Δ81

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has neoplasia | somatic clone | third instar larval stage phenotype, non-suppressible by Atg13+tCH322-168O18, Atg13Δ81/Atg13Δ81

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has abnormal size | somatic clone | third instar larval stage phenotype, non-suppressible | partially | cell autonomous | cell non-autonomous | somatic clone by Atg13Δ81/Atg13Δ81

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has abnormal size | somatic clone | third instar larval stage phenotype, non-suppressible by Atg13+tCH322-168O18, Atg13Δ81/Atg13Δ81

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has increased cell death | somatic clone | third instar larval stage phenotype, non-suppressible | cell autonomous | cell non-autonomous by Atg13Δ81/Atg13Δ81

      (Katheder et al., 2017)
      Enhancer of
      Statement
      Reference

      scrib[+]/scrib673 is an enhancer of abnormal planar polarity phenotype of Vangstbm-153

      (Courbard et al., 2009)

      scrib[+]/scrib673 is an enhancer of visible phenotype of Scer\GAL4GMR.PF, crbintra.UAS

      (Tanentzapf and Tepass, 2003)
      NOT Enhancer of
      Statement
      Reference

      scrib673 is a non-enhancer of visible phenotype of RetMEN2B.GMR

      (Read et al., 2005)
      Suppressor of
      Statement
      Reference

      scrib673 is a suppressor of visible phenotype of RetMEN2A.GMR

      (Read et al., 2005)

      scrib[+]/scrib673 is a suppressor of visible phenotype of CycEJP

      (Brumby et al., 2004)
      NOT Suppressor of
      Statement
      Reference

      scrib673 is a non-suppressor of visible phenotype of RetMEN2B.GMR

      (Read et al., 2005)
      Other
      Statement
      Reference

      Egfr2.A887T.UAS, Scer\GAL4Act.PU, scrib673 has neoplasia | somatic clone | larval stage phenotype

      (Sheng and Du, 2020)

      Egfr2.A887T.UAS, Scer\GAL4Act.PU, scrib673 has increased occurrence of cell division | somatic clone | larval stage phenotype

      (Sheng and Du, 2020)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has neoplasia | somatic clone | third instar larval stage phenotype

      (Katheder et al., 2017)

      Atg13Δ81, Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has abnormal size | somatic clone | third instar larval stage phenotype

      (Katheder et al., 2017)

      Atg13Δ81, Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has decreased cell number | somatic clone | third instar larval stage phenotype

      (Katheder et al., 2017)

      Atg13Δ81, Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has neoplasia | somatic clone | third instar larval stage phenotype

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has abnormal size | somatic clone | cell autonomous phenotype

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has increased cell number | somatic clone | third instar larval stage phenotype

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has abnormal size | somatic clone | third instar larval stage phenotype

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has increased cell death | somatic clone | third instar larval stage phenotype

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has lethal | somatic clone | larval stage phenotype

      (Jiang et al., 2011)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has neoplasia | somatic clone phenotype

      (Jiang et al., 2011)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673, sds22UAS.cJa has lethal | somatic clone | P-stage phenotype

      (Jiang et al., 2011)
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      Egfr2.A887T.UAS, Scer\GAL4Act.PU, scrib673 has larval brain | somatic clone | larval stage phenotype, suppressible by psidin55D4/psidin55D4

      (Sheng and Du, 2020)

      Egfr2.A887T.UAS, Scer\GAL4Act.PU, scrib673 has gonad | somatic clone | larval stage phenotype, suppressible by psidin55D4/psidin55D4

      (Sheng and Du, 2020)

      Egfr2.A887T.UAS, Scer\GAL4Act.PU, scrib673 has larval brain | somatic clone | larval stage phenotype, suppressible by psidinS678D.UAS.Tag:HA, psidin55D4/psidin55D4/Scer\GAL4Act.PU

      (Sheng and Du, 2020)

      Egfr2.A887T.UAS, Scer\GAL4Act.PU, scrib673 has gonad | somatic clone | larval stage phenotype, suppressible by psidinS678D.UAS.Tag:HA, psidin55D4/psidin55D4/Scer\GAL4Act.PU

      (Sheng and Du, 2020)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has eye-antennal disc | somatic clone | third instar larval stage phenotype, suppressible | cell autonomous | cell non-autonomous | partially | somatic clone by Atg13Δ81/Atg13Δ81

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has eye-antennal disc | somatic clone | third instar larval stage phenotype, suppressible by Atg13+tCH322-168O18, Atg13Δ81/Atg13Δ81

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has eye-antennal disc | somatic clone | third instar larval stage phenotype, suppressible | cell non-autonomous | partially by Atg14Δ5.2/Atg14Δ5.2

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has eye-antennal disc | somatic clone | third instar larval stage phenotype, suppressible | partially | somatic clone by Atg13Δ81/Atg13Δ81/Atg14Δ5.2/Atg14Δ5.2

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has eye-antennal disc | somatic clone | third instar larval stage phenotype, suppressible | partially by Atg13ey.3.5, Atg13Δ81/Atg13Δ81

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has eye-antennal disc | somatic clone | cell non-autonomous | third instar larval stage phenotype, suppressible | partially by Atg13Δ81/Atg13Δ81

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has autophagosome | somatic clone | cell non-autonomous | third instar larval stage phenotype, suppressible by Atg13Δ81/Atg13Δ81

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has larval somatic muscle cell | somatic clone | cell non-autonomous | third instar larval stage phenotype, suppressible by Atg13Δ81/Atg13Δ81

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has embryonic/larval midgut | somatic clone | cell non-autonomous | third instar larval stage phenotype, suppressible by Atg13Δ81/Atg13Δ81

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has embryonic/larval fat body | somatic clone | cell non-autonomous | third instar larval stage phenotype, suppressible by Atg13Δ81/Atg13Δ81

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has autophagosome | somatic clone | cell non-autonomous | third instar larval stage phenotype, suppressible | cell non-autonomous | somatic clone by bskDN.UAS.cUa, Scer\GAL4Act.PU

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has embryonic/larval midgut | somatic clone | cell non-autonomous | third instar larval stage phenotype, suppressible | cell non-autonomous | somatic clone by bskDN.UAS.cUa, Scer\GAL4Act.PU

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has autophagosome | somatic clone | cell non-autonomous | third instar larval stage phenotype, suppressible | somatic clone by domeΔCYT.UAS, Scer\GAL4Act.PU

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has eye-antennal disc | somatic clone | cell non-autonomous | third instar larval stage phenotype, suppressible | cell non-autonomous | somatic clone by domeΔCYT.UAS, Scer\GAL4Act.PU

      (Katheder et al., 2017)

      scrib673 has eye disc | somatic clone phenotype, suppressible by Scer\GAL4Tub.PU/hthHMS01112

      (Khan et al., 2013)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has eye-antennal disc | somatic clone phenotype, suppressible | somatic clone by sds22UAS.cJa, Scer\GAL4Act.PU

      (Jiang et al., 2011)

      scrib673 has eye-antennal disc | somatic clone phenotype, suppressible by Scer\GAL4Act.PU/sds22UAS.cJa

      (Jiang et al., 2011)

      scrib673 has eye | somatic clone phenotype, suppressible by Scer\GAL4Act.PU/sds22UAS.cJa

      (Jiang et al., 2011)

      scrib673 has eye | somatic clone phenotype, suppressible by Scer\GAL4Act5C.PI/hepAct.UAS

      (Uhlirova et al., 2005)
      NOT suppressed by
      Statement
      Reference

      scrib5/scrib673 has wing disc | larval stage phenotype, non-suppressible by Scer\GAL4ap-md544/α-CatUAS.Tag:MYC

      (Huang et al., 2023)

      scrib5/scrib673 has wing disc | larval stage phenotype, non-suppressible by scrib::α-CatLRR.UAS.Tag:MYC/Scer\GAL4ap-md544

      (Huang et al., 2023)

      scrib5/scrib673 has wing disc | larval stage phenotype, non-suppressible by Scer\GAL4ap-md544/wtsUAS.Tag:MYC

      (Huang et al., 2023)

      Egfr2.A887T.UAS, Scer\GAL4Act.PU, scrib673 has larval brain | somatic clone | larval stage phenotype, non-suppressible by psidinS678A.UAS.Tag:HA, psidin55D4/psidin55D4/Scer\GAL4Act.PU

      (Sheng and Du, 2020)

      Egfr2.A887T.UAS, Scer\GAL4Act.PU, scrib673 has gonad | somatic clone | larval stage phenotype, non-suppressible by psidinS678A.UAS.Tag:HA, psidin55D4/psidin55D4/Scer\GAL4Act.PU

      (Sheng and Du, 2020)

      Egfr2.A887T.UAS, Scer\GAL4Act.PU, scrib673 has larval brain | somatic clone | larval stage phenotype, non-suppressible by psidinUAS.cKa, psidin55D4/psidin55D4/Scer\GAL4Act.PU

      (Sheng and Du, 2020)

      Egfr2.A887T.UAS, Scer\GAL4Act.PU, scrib673 has gonad | somatic clone | larval stage phenotype, non-suppressible by psidinUAS.cKa, psidin55D4/psidin55D4/Scer\GAL4Act.PU

      (Sheng and Du, 2020)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has eye-antennal disc | somatic clone | third instar larval stage phenotype, non-suppressible | cell autonomous | cell non-autonomous | partially | somatic clone by Atg13Δ81/Atg13Δ81

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has eye-antennal disc | somatic clone | third instar larval stage phenotype, non-suppressible by Atg13+tCH322-168O18, Atg13Δ81/Atg13Δ81

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has eye-antennal disc | somatic clone | cell non-autonomous | third instar larval stage phenotype, non-suppressible by Atg13+tCH322-168O18, Atg13Δ81/Atg13Δ81

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has autophagosome | somatic clone | cell non-autonomous | third instar larval stage phenotype, non-suppressible | cell non-autonomous | somatic clone by bskDN.UAS.cUa, Scer\GAL4Act.PU

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has larval somatic muscle cell | somatic clone | cell non-autonomous | third instar larval stage phenotype, non-suppressible | cell non-autonomous | somatic clone by bskDN.UAS.cUa, Scer\GAL4Act.PU

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has embryonic/larval fat body | somatic clone | cell non-autonomous | third instar larval stage phenotype, non-suppressible | cell non-autonomous | somatic clone by bskDN.UAS.cUa, Scer\GAL4Act.PU

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has autophagosome | somatic clone | cell non-autonomous | third instar larval stage phenotype, non-suppressible | cell autonomous by Stat92E85C9/Stat92E85C9

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has eye-antennal disc | somatic clone | cell non-autonomous | third instar larval stage phenotype, non-suppressible | cell autonomous by Stat92E85C9/Stat92E85C9

      (Katheder et al., 2017)
      Enhancer of
      Statement
      Reference

      scrib[+]/scrib673 is an enhancer of egg chamber phenotype of RhoGAP19Dunspecified

      (Fic et al., 2021)

      scrib[+]/scrib673 is an enhancer of ommatidium phenotype of Vangstbm-153

      (Courbard et al., 2009)

      scrib[+]/scrib673 is an enhancer of eye phenotype of Scer\GAL4GMR.PF, crbintra.UAS

      (Tanentzapf and Tepass, 2003)
      NOT Enhancer of
      Statement
      Reference

      scrib673 is a non-enhancer of eye phenotype of RetMEN2B.GMR

      (Read et al., 2005)
      Suppressor of
      NOT Suppressor of
      Statement
      Reference

      scrib673 is a non-suppressor of eye phenotype of RetMEN2B.GMR

      (Read et al., 2005)
      Other
      Statement
      Reference

      Egfr2.A887T.UAS, Scer\GAL4Act.PU, scrib673 has larval brain | somatic clone | larval stage phenotype

      (Sheng and Du, 2020)

      Egfr2.A887T.UAS, Scer\GAL4Act.PU, scrib673 has gonad | somatic clone | larval stage phenotype

      (Sheng and Du, 2020)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has autophagosome | somatic clone | cell non-autonomous | third instar larval stage phenotype

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has larval somatic muscle cell | somatic clone | cell non-autonomous | third instar larval stage phenotype

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has embryonic/larval midgut | somatic clone | cell non-autonomous | third instar larval stage phenotype

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has embryonic/larval fat body | somatic clone | cell non-autonomous | third instar larval stage phenotype

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has mitochondrion | somatic clone | cell autonomous phenotype

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Tub.PU, scrib673 has wing disc | somatic clone | cell non-autonomous | third instar larval stage phenotype

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Tub.PU, scrib673 has autophagosome | somatic clone | cell non-autonomous | third instar larval stage phenotype

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has eye-antennal disc | somatic clone | cell non-autonomous | third instar larval stage phenotype

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has eye-antennal disc | somatic clone | third instar larval stage phenotype

      (Katheder et al., 2017)

      Ras85DV12.UAS, Scer\GAL4Act.PU, scrib673 has eye-antennal disc | somatic clone phenotype

      (Jiang et al., 2011)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      EyFLP-induced clones that both express Egfr2.A887T.UAS under the control of Scer\GAL4Act.PU and are scrib673/scrib673 form cephalic and gonadal tumors. The gonadal tumors are highly proliferative compared to neighboring control tissue.

      (Sheng and Du, 2020)

      Clones homozygous for scrib673 and expressing Ras85DV12.Scer\UAS under the control of Scer\GAL4Act.PU in third instar larval eye-antennal imaginal discs form invasive tumors, which display increased proportions of clone cells entering the cell cycle (but not undergoing mitosis), undergoing apoptosis and presenting mitochondrial defects - i.e., significant increases in the average mass and number of mitochondria per cell, a strong increase in the proportion of burst mitochondria, and a significant increase in the levels of reactive oxygen species, as compared to controls; the presence of these tumor clones also leads to a cell non-autonomous increase in autophagosomes (assessed by a Atg8a fluorescence reporter) in both neighboring disc tissue and distant tissues - i.e. somatic muscles, midgut and fat body - and in significant decreases in the respiratory capacity in both tumor and neighboring disc tissue, as compared to controls.

      scrib673-homozygous, Ras85DV12.Scer\UAS-expressing eye-antennal imaginal disc clone tumors are significantly suppressed by Atg13Δ81 homozygosity in clone cells (leading to reductions in the proportions of clone cells entering the cell cycle, but not undergoing mitosis or apoptosis, and leading to a reduction in the tumor size, but not invasiveness), in neighboring disc cells (leading to reductions in the tumor size and in the proportion of cell entering the cell cycle, but not undergoing mitosis or apoptosis), in the full organism except the clone cells (leading to reductions in the tumor size and invasiveness), or in the full organism (leading to reductions in the proportions of cells entering the cell cycle and undergoing mitosis, but not undergoing apoptosis, and leading to reductions in the tumor size and invasiveness); these tumors are also significantly suppressed by Atg14Δ5.2 homozygosity in the neighboring disc cells (leading to a reduction in the tumor size) or by the combination of Atg13Δ81 homozygosity in the tumor cells and Atg14Δ5.2 homozygosity in the neighboring disc cells. One copy of Atg13+tCH322-168O18 in the full organism suppresses the decrease in tumor size, but not invasiveness, resulting from Atg13Δ81 homozygosity in the full organism or in the full organism apart from the clone cells; one copy of Atg13ey.3.5 in the full organism also partially suppresses the decrease in tumor size resulting from Atg13Δ81 homozygosity. These tumors grow to significantly smaller sizes when grafted into the abdomen of either Atg14Δ5.2/Atg14EY14568 transheterozygous adult hosts (but not if the hosts possess one copy of Atg14+tCH322-175F03) or Atg8ad4 homozygous adult hosts, as compared to control hosts.

      The cell non-autonomous increase in autophagosomes resulting from the presence of clones homozygous for scrib673 and expressing Ras85DV12.Scer\UAS under the control of Scer\GAL4Act.PU in third instar larval eye-antennal imaginal discs is suppressed by Atg13Δ81 homozygosity in either the full organism (suppression in distant tissues - somatic muscles, midgut and fat body) or in the full organism apart from the clone cells (suppression in neighboring disc tissue), or by the clonal co-expression of either domeΔCYT.Scer\UAS (suppression in neighboring disc tissue) or bskDN.Scer\UAS.cUa (suppression in the midgut, but not in the somatic muscles or the fat body), but not by Stat92E85C9 homozygosity in the full organism apart from the clone cells.

      Clones homozygous for scrib673 and expressing Ras85DV12.Scer\UAS under the control of Scer\GAL4tub.PU in third instar larval wing imaginal discs lead to a cell non-autonomous increase in autophagosomes (assessed by a Atg8a fluorescence reporter) in neighboring disc tissue, as compared to controls.

      (Katheder et al., 2017)

      Expression of hthHMS01112 under the control of Scer\GAL4tub.PU suppresses the neoplastic transformation seen in scrib673 mutant eye disc clones.

      (Khan et al., 2013)

      Expression of Ras85DV12.Scer\UAS under the control of Scer\GAL4Act.PU in clones in the eye-antennal disc (where the clones are also homozygous for scrib673) results in strong tumour growth at 7 days AEL. These animals grow as larvae until 13 days AEL and die before pupation. Co-expression of sds22Scer\UAS.cJa in these clones strongly suppresses the tumour growth phenotype in all clones observed at 7 days AEL and most of these animals can pupate, but die as early pupae.

      The eye-antennal disc defects seen in larvae carrying homozygous scrib673 clones and the eye defects seen in adult escapers carrying homozygous scrib673 clones are suppressed by expression of sds22Scer\UAS.cJa under the control of Scer\GAL4Act.PU in the clones.

      (Jiang et al., 2011)

      The cuticle defects observed when bazS980A.Scer\UAS.T:Avic\GFP is expressed under the control of Scer\GAL4arm.PS are strongly suppressed in a scrib673 mutant background.

      (Krahn et al., 2010)

      Heterozygosity for scrib673 enhances the frequency of ommatidia that show planar cell polarity defects in Vangstbm-153 homozygotes.

      (Courbard et al., 2009)

      Expression of hepAct.Scer\UAS in scrib673 mutant clones under the control of Scer\GAL4Act5C.PI suppressed the scrib673 phenotype to revert to an essentially wild-type appearance, without the marked lesions resulting from scrib-deficient tissue. Significantly, these phenotypically rescued eyes do not contain any clonal cells, suggesting that JNK activity (increased by expression of hep) counteracts the outgrowth of scrib1 tissue or causes the removal of the mutant cells. These eyes are the same size as wild-type.

      (Uhlirova et al., 2005)

      The epithelial defects of crb11A2 embryos are markedly suppressed if the embryos are also mutant for scrib673; continuous patches of cuticle are formed in the double mutant embryos and the xonula adherens resembles that of wild-type embryos.

      (Bilder et al., 2003)

      Embryos lacking both crb and scrib maternal and zygotic function (derived from scrib673 crb2 homozygous germline clones) have a phenotype similar to that of embryos derived from scrib673 homozygous germline clones (lacking scrib maternal and zygotic function). Formation of the zonula adherens is largely rescued in crb2 scrib673 double mutant embryos.

      (Tanentzapf and Tepass, 2003)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to complement

      scrib882

      (Zeitler et al., 2004)

      scribird15

      (Zeitler et al., 2004)

      scrib1

      (Zeitler et al., 2004)

      scribdt12

      (Zeitler et al., 2004)

      scribdt14

      (Zeitler et al., 2004)

      scribdt6

      (Zeitler et al., 2004)

      scribsmi97B

      (Ganguly et al., 2003)

      scribsmi97B

      (Ganguly et al., 2001)

      scribird15

      (Wu et al., 2001)

      scribdt12

      (Kidd et al., 1999)

      scribdt14

      (Kidd et al., 1999)

      scribdt6

      (Kidd et al., 1999)
      Rescued by

      scrib5/scrib673 is rescued by Scer\GAL4ptc-559.1/scribUAS.cGa.Tag:MYC

      (Huang et al., 2023)

      scrib5/scrib673 is rescued by scribUAS.cGa.Tag:MYC/Scer\GAL4ap-md544

      (Huang et al., 2023)

      scribird15/scrib673 is rescued by Scer\GAL469B/scribUASp.GFP

      (Zeitler et al., 2004)

      scribird15/scrib673 is rescued by scribΔPDZ3.4.UASp.GFP/Scer\GAL469B

      (Zeitler et al., 2004)

      scribird15/scrib673 is rescued by scribUAS.cBa/Scer\GAL469B

      (Zeitler et al., 2004)

      scrib673 is rescued by Scer\GAL4futsch-C380/scribUAS.cBa

      (Roche et al., 2002)

      scrib673 is rescued by scribUAS.cBa/Scer\GAL4BG487

      (Roche et al., 2002)
      Partially rescued by

      scribird15/scrib673 is partially rescued by scribΔPDZs.UASp.GFP/Scer\GAL469B

      (Zeitler et al., 2004)

      scribird15/scrib673 is partially rescued by scrib4.UASp.GFP/Scer\GAL469B

      (Zeitler et al., 2004)

      scribdt6/scrib673 is partially rescued by scribΔPDZs.UASp.GFP/Scer\GAL469B

      (Zeitler et al., 2004)

      scribdt6/scrib673 is partially rescued by scrib4.UASp.GFP/Scer\GAL469B

      (Zeitler et al., 2004)

      scribj7B3/scrib673 is partially rescued by scribΔPDZs.UASp.GFP/Scer\GAL469B

      (Zeitler et al., 2004)

      scribj7B3/scrib673 is partially rescued by scrib4.UASp.GFP/Scer\GAL469B

      (Zeitler et al., 2004)
      Not rescued by

      scrib5/scrib673 is not rescued by scribLRR.UAS.cGa.Tag:MYC/Scer\GAL4ap-md544

      (Huang et al., 2023)

      scrib5/scrib673 is not rescued by Scer\GAL4ap-md544/scribdLRR.UAS.cGa.Tag:MYC

      (Huang et al., 2023)

      scribird15/scrib673 is not rescued by scribΔLRR.UASp.GFP/Scer\GAL469B

      (Zeitler et al., 2004)

      scribird15/scrib673 is not rescued by scribΔPDZs.UASp.GFP/Scer\GAL469B/scribΔLRR.UASp.Tag:Myr(Src42A),GFP

      (Zeitler et al., 2004)

      scribird15/scrib673 is not rescued by Scer\GAL469B/scribΔLRR.UASp.Tag:Myr(Src42A),GFP

      (Zeitler et al., 2004)

      scribird15/scrib673 is not rescued by scribL223Q.UASp.GFP/Scer\GAL469B

      (Zeitler et al., 2004)

      scribdt6/scrib673 is not rescued by scribΔLRR.UASp.GFP/Scer\GAL469B

      (Zeitler et al., 2004)

      scribdt6/scrib673 is not rescued by Scer\GAL469B/scribΔLRR.UASp.Tag:Myr(Src42A),GFP

      (Zeitler et al., 2004)

      scribj7B3/scrib673 is not rescued by scribΔLRR.UASp.GFP/Scer\GAL469B

      (Zeitler et al., 2004)

      scribj7B3/scrib673 is not rescued by Scer\GAL469B/scribΔLRR.UASp.Tag:Myr(Src42A),GFP

      (Zeitler et al., 2004)

      Df(3R)Tl-X/scrib673 is not rescued by Scer\GAL4futsch-C380/scribUAS.cBa

      (Roche et al., 2002)
      Comments

      Expression of scribScer\UAS.cBa under the control of Scer\GAL469B fully rescues the cell polarization and disc overgrowth phenotypes of scrib673/scribird15 transheterozygotes.

      Expression of scribScer\UAS.P\T.T:Avic\GFP under the control of Scer\GAL469B fully rescues the cell polarization and disc overgrowth phenotypes of scrib673/scribird15 transheterozygotes.

      Expression of scribΔLRR.Scer\UAS.P\T.T:Avic\GFP under the control of Scer\GAL469B fails to rescue the cell polarization and disc overgrowth phenotypes of scrib673/scribird15 transheterozygotes.

      Expression of scribL223Q.Scer\UAS.P\T.T:Avic\GFP under the control of Scer\GAL469B fails to rescue the cell polarization and disc overgrowth phenotypes of scrib673/scribird15 transheterozygotes.

      Expression of scribΔLRR.Scer\UAS.P\T.T:Myr6,T:Avic\GFP under the control of Scer\GAL469B fails to rescue the cell polarization and disc overgrowth phenotypes of scrib673/scribird15 transheterozygotes.

      Expression of scrib4.Scer\UAS.P\T.T:Avic\GFP under the control of Scer\GAL469B fully rescues the cell polarization phenotype but only partially rescues the disc overgrowth phenotype of scrib673/scribird15 transheterozygotes.

      Expression of scribΔPDZs.Scer\UAS.P\T.T:Avic\GFP under the control of Scer\GAL469B fully rescues the cell polarization phenotype but only partially rescues the disc overgrowth phenotype of scrib673/scribird15 transheterozygotes.

      Expression of scribΔPDZ3.4.Scer\UAS.P\T.T:Avic\GFP under the control of Scer\GAL469B fully rescues the cell polarization and disc overgrowth phenotypes of scrib673/scribird15 transheterozygotes.

      Expression of scribΔLRR.Scer\UAS.P\T.T:Avic\GFP under the control of Scer\GAL469B fails to rescue the cell polarization and disc overgrowth phenotypes of scrib673/scribdt6 transheterozygotes.

      Expression of scribΔLRR.Scer\UAS.P\T.T:Avic\GFP under the control of Scer\GAL469B fails to rescue the cell polarization and disc overgrowth phenotypes of scrib673/scribj7B3 transheterozygotes.

      Expression of scribΔLRR.Scer\UAS.P\T.T:Myr6,T:Avic\GFP under the control of Scer\GAL469B fails to rescue the cell polarization and disc overgrowth phenotypes of scrib673/scribdt6 transheterozygotes.

      Expression of scribΔLRR.Scer\UAS.P\T.T:Myr6,T:Avic\GFP under the control of Scer\GAL469B fails to rescue the cell polarization and disc overgrowth phenotypes of scrib673/scribj7B3 transheterozygotes.

      Co-expression of scribΔLRR.Scer\UAS.P\T.T:Myr6,T:Avic\GFP and scrib<up>ΔPDZs.Scer\UAS.P\T. T:Avic \GFP</up> under the control of Scer\GAL469B fails to rescue the cell polarization and disc overgrowth phenotypes of scrib673/scribird15 transheterozygotes, even when expression levels are increased via raising larvae at 29 oC.

      Expression of scrib4.Scer\UAS.P\T.T:Avic\GFP or scribΔPDZs.Scer\UAS.P\T.T:Avic\GFP under the control of Scer\GAL469B provides a more complete rescue of the size phenotype of scrib673/scribdt6 and scrib673/scribj7B3 transheterozygous discs compared to scrib null discs. Increasing the level of transgene expression via raising larvae at 29 oC further increases this rescue. Moreover, the apicolateral enhancement of septate junction markers is restored and cell shape is columnar rather than cuboidal in rescued scrib673/scribdt6 discs.

      (Zeitler et al., 2004)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (8)
      References (49)