Allele Dmel\piwi^5'T:Hsap\MYC

General Information
Symbol	Dmel\piwi^5'T:Hsap\MYC	Species	D. melanogaster
Name		FlyBase ID	FBal0103600
Feature type	allele	Associated gene	Dmel\piwi

Allele class
Mutagen	in vitro construct - coding region fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Cox et al., 2000)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	piwi^+t6.8 (Cox et al., 2000)
Nature of the lesion	Statement Reference Construct: A T:Hsap\MYC tag has been inserted at the 5' end of the piwi open reading frame (between amino acid residues 3 and 4). (Cox et al., 2000)
Carried in construct	P{5'-myc-piwi} (Cox et al., 2000, Smulders-Srinivasan and Lin, 2003, Szakmary et al., 2005, King et al., 2001, Megosh et al., 2006, Megosh et al., 2006, Yin and Lin, 2007, Brower-Toland et al., 2007, Chau et al., 2009, Szakmary et al., 2009, Gangaraju et al., 2011)
Tagged with	T:Hsap\MYC (Cox et al., 2000, King et al., 2001, Smulders-Srinivasan and Lin, 2003, Szakmary et al., 2005)
Cytology
Phenotypic Data
Phenotypic Class
	male fertile (Szakmary et al., 2009) wild-type (Cox et al., 2000)
Phenotype Manifest In
	pole cell \| maternal effect \| supernumerary (Megosh et al., 2006)
Detailed Description
	Statement Reference Cycle 14 embryos produced by females carrying 4 copies of the piwi^5'T:Hsap\MYC transgene have around 50 pole cells, while embryos produced by females carrying 6 copies of piwi^5'T:Hsap\MYC have around 66 pole cells, compared to 24 pole cells in wild-type embryos. The embryos from the transgene-carrying mothers show no increase in the number of somatic cells and no defects in either abdominal patterning or embryonic polarity. (Megosh et al., 2006)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference The ability of geldanamycin to increase the frequency of the ectopic eye outgrowth phenotype in Kr[If-1] homozygous females is reduced if the females are also carrying piwi[5'T:Hsap\MYC]. (Gangaraju et al., 2011)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	piwi^5'T:Hsap\MYC rescues piwi¹ (Cox et al., 2000)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	piwi^5'T:Hsap\MYC
Name Synonym
Secondary FlyBase IDs

References ( 11 )
Research paper	Gangaraju et al., 2011, Nat. Genet. 43(2): 153--158 Drosophila Piwi functions in Hsp90-mediated suppression of phenotypic variation. [FBrf0212873] Chau et al., 2009, Genetics 182(1): 121--132 Sex-lethal Facilitates the Transition From Germline Stem Cell to Committed Daughter Cell in the Drosophila Ovary. [FBrf0207693] Szakmary et al., 2009, J. Cell Biol. 185(4): 613--627 The Yb protein defines a novel organelle and regulates male germline stem cell self-renewal in Drosophila melanogaster. [FBrf0207998] Brower-Toland et al., 2007, Genes Dev. 21(18): 2300--2311 Drosophila PIWI associates with chromatin and interacts directly with HP1a. [FBrf0201020] Yin and Lin, 2007, Nature 450(7167): 304--308 An epigenetic activation role of Piwi and a Piwi-associated piRNA in Drosophila melanogaster. [FBrf0202885] Megosh et al., 2006, Curr. Biol. 16(19): 1884--1894 The role of PIWI and the miRNA machinery in Drosophila germline determination. [FBrf0191897] Szakmary et al., 2005, Curr. Biol. 15(2): 171--178 Regulatory relationship among piwi, pumilio, and bag-of-marbles in Drosophila germline stem cell self-renewal and differentiation. [FBrf0183821] Smulders-Srinivasan and Lin, 2003, Genetics 165(4): 1971--1991 Screens for piwi suppressors in Drosophila identify dosage-dependent regulators of germline stem cell division. [FBrf0167655] King et al., 2001, Mol. Cell 7(3): 497--508 Yb modulates the divisions of both germline and somatic stem cells through piwi- and hh-mediated mechanisms in the Drosophila ovary. [FBrf0137038] Cox et al., 2000, Development 127(3): 503--514 piwi encodes a nucleoplasmic factor whose activity modulates the number and division rate of germline stem cells. [FBrf0122994]
Supplementary material	Megosh et al., 2006, Curr. Biol. 16(19): Supplemental Data. The role of PIWI and the miRNA machinery in Drosophila germline determination. [FBrf0198728]

Allele Dmel\piwi5'T:Hsap\MYC

Allele Dmel\piwi^5'T:Hsap\MYC