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General Information
Symbol
Dmel\RafF20.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0103603
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Δ-RafF20
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a truncated form of phl, which lacks amino acids 2 to 431. Produces a constitutively active protein.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of phlF20.Scer\UAS under the control of Scer\GAL4amn-c651 reduces pupal body size. This genotype has no effect on prothoracic gland cell size.

When phlF20.Scer\UAS is driven by Scer\GAL4hs.PB and heatshocked for 30 minutes at 37oC, large territories of trichomes are transformed into bracts; in legs, the proximal femur to the claw, all trichomes are transformed into bracts. In the notum, the region between the dorsocentral bristles also differentiate multiple bracts. In wings, only the cells of the dorsal and ventral proximal costa differentiate as bracts; the rest of the wing cells are not affected, apart from a small area in the dorsal hinge that also that also differentiates a patch of bracts. In the halteres multiple bracts cover the dorsal and ventral anterior pedicelem. The prothorax also differentiates a small patch of bracts. In the head bracts are found at the base of the arista, and in the gena and the supraorbital region. The size and shape of bracts was constant in different parts of the fly. When phlF20.Scer\UAS, Scer\GAL4hs.PB flies are heatshock induced at 8 hours APF differentiate a large number of bracts, with almost all the trichomes from the above-defined competent areas transformed. Heat sock at later stages yield a reduced density of bracts, and earlier heat shock produces a pattern of regions of normal trichomes surrounding patches of bracts are mainly areas among bristles.

Expression of phlF20.Scer\UAS (driven by Scer\GAL4GMR.PF) leads to the recruitment of supernumerary R7 photoreceptor cells. When driven by Scer\GAL4604, flies also have reduced viability, and wing phenotypes when raised at 25oC. The central region of wing vein L3 is deleted, there is a partial loss of vein L5 and an overall reduction in wing size.

Flies expressing phlF20.Scer\UAS under the control of Scer\GAL4604 have reduced viability and show wing phenotypes; they have ectopic veins and sensilla and a loss of endogenous veins. The wing may also be reduced in size.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Statement
Reference

Scer\GAL4604, RafF20.UAS has wing vein L3 phenotype, suppressible by Sps1k11320/SelD[+]

Scer\GAL4604, RafF20.UAS has wing vein L5 phenotype, suppressible by Sps1k11320/SelD[+]

Scer\GAL4604, RafF20.UAS has wing phenotype, suppressible | partially by Sps1k11320/SelD[+]

Additional Comments
Genetic Interactions
Statement
Reference

When animals containing Poxnhs.sev and phlF20.Scer\UAS (driven by Scer\GAL4hs.PB) and heatshocked, an additive phenotype is seen, mechanosensory bristles are transformed into chemosensory bristles and all epidermal cells are transformed into bracts. A similar effect is seen by the combination of Poxnhs.sev and Poxnhs.sev (driven by Scer\GAL4hs.PB).

The addition of SelDk11320/+ to phlF20.Scer\UAS, Scer\GAL4604 flies leads to almost wild-type vein pattern, although wing size is still reduced.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
RafF20.Scer\UAS
RafF20.UAS
phlF20.Scer\UAS
Name Synonyms
Secondary FlyBase IDs
    References (5)