FlyBase Allele Report: Dmel\kn[UAS.cMa]

General Information

Symbol

Dmel\kn^UAS.cMa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Mohler

FlyBase ID

FBal0103782

Feature type

allele

Associated gene

Dmel\kn

Associated Insertion(s)

Carried in Construct

P{UAS-kn.M}

Also Known As

UAS-kn

Key Links

Transgenic product class

wild_type

(Mohler et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Mohler et al., 2000)

Mutagen

in vitro construct

(Mohler et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-kn.M}

Cytology

Description

UASt regulatory sequences drive expression of the entire kn open reading frame.

(Mohler et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4²²¹

(Jinushi-Nakao et al., 2007)

abnormal neuroanatomy, with Scer\GAL4^smid-C161

(Jinushi-Nakao et al., 2007)

abnormal neuroanatomy | somatic clone, with Scer\GAL4^21-7

(Ye et al., 2011)

visible, with Scer\GAL4^Bx-MS1096

(Mohler et al., 2000)

visible, with Scer\GAL4^dpp.blk1

(Mohler et al., 2000)

Phenotype Manifest In

dendrite, with Scer\GAL4²²¹

(Jinushi-Nakao et al., 2007)

dendrite, with Scer\GAL4^smid-C161

(Jinushi-Nakao et al., 2007)

dendritic arborizing neuron | somatic clone, with Scer\GAL4^21-7

(Ye et al., 2011)

larval multidendritic class III neuron, with Scer\GAL4^smid-C161

(Jinushi-Nakao et al., 2007)

larval multidendritic class II neuron, with Scer\GAL4^smid-C161

(Jinushi-Nakao et al., 2007)

larval multidendritic class I neuron, with Scer\GAL4²²¹

(Jinushi-Nakao et al., 2007)

ommatidium | pupal stage, with Scer\GAL4^GMR.PU

(Na et al., 2015)

wing vein L2, with Scer\GAL4^Bx-MS1096

(Mohler et al., 2000)

wing vein L3, with Scer\GAL4^Bx-MS1096

(Mohler et al., 2000)

wing vein L3 | distal, with Scer\GAL4^dpp.blk1

(Mohler et al., 2000)

wing vein L4 | distal, with Scer\GAL4^Bx-MS1096

(Mohler et al., 2000)

wing vein L4 | proximal, with Scer\GAL4^Bx-MS1096

(Mohler et al., 2000)

wing vein L5 | distal, with Scer\GAL4^Bx-MS1096

(Mohler et al., 2000)

wing vein L5 | proximal, with Scer\GAL4^Bx-MS1096

(Mohler et al., 2000)

Detailed Description

Statement

Reference

Expression of kn^Scer\UAS.cMa under the control of Scer\GAL4^GMR.PU results in severely disrupted ommatidial patterning in the pupal eye.

(Na et al., 2015)

Overexpression of kn^Scer\UAS.cMa in class I da neurons (using MARCM and Scer\GAL4^21-7) results in a significant increase in total dendrite length.

(Ye et al., 2011)

Ectopic expression of kn^Scer\UAS.cMa (under the control of Scer\GAL4²²¹) significantly increases the number of dendrite termini by 19%, total arbor length by 46%, and arbor area by 18%. In class I neurons expressing kn^Scer\UAS.cMa, the extra length of dendrite is preferentially concentrated in the last segments of the dendritic arbor.

Ectopic expression of kn^Scer\UAS.cMa (under the control of Scer\GAL4^smid-C161) in class II neurons results in a large increase in dendritic arbor complexity in third instar larvae. In the same way, ectopic kn^Scer\UAS.cMa expression increases class III neuron (v'pda) total arbor length by 19%. The number of filopodia/spikes, defined as very thin and short lateral projections from the main dendrite trunk, is highly reduced.

Ectopic expression of kn^Scer\UAS.cMa (under the control of Scer\GAL4^109(2)80) in class IV neurons results in a strong reduction in the number of filopodia/spikes in a 75υm radius around the cell body to 23% of wild-type levels.

(Jinushi-Nakao et al., 2007)

Expression of kn^Scer\UAS.cMa under the control of Scer\GAL4^Bx-MS1096 results in wings lacking vein 3, the distal portion of vein 4 and the proximal portion of vein 5. Vein 2, the proximal part of vein 4 and the distal part of vein 5 are diffuse, wide and plexate. Expression of kn^Scer\UAS.cMa under the control of Scer\GAL4^dpp.blk1 often results in the disruption of wing vein 3; the distal portion is disrupted and shifted anteriorly, meeting the wing margin considerably anterior to the posterior-most socketed bristle (in contrast to wild type, where most socketed bristles are anterior to vein 3). The distance from vein 4 to the posterior-most socketed bristle is unchanged from wild type.

(Mohler et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4²²¹, kn^UAS.cMa has abnormal neuroanatomy phenotype, enhanceable by Rac1^UAS.cLa, Scer\GAL4²²¹

(Jinushi-Nakao et al., 2007)

Scer\GAL4²²¹, kn^UAS.cMa has abnormal neuroanatomy phenotype, enhanceable by ct^UAS.cPa/Scer\GAL4²²¹

(Jinushi-Nakao et al., 2007)

Suppressed by

Statement

Reference

Scer\GAL4^21-7, kn^UAS.cMa has abnormal neuroanatomy | somatic clone phenotype, suppressible by dar1³⁰¹⁰

(Ye et al., 2011)

Scer\GAL4²²¹, kn^UAS.cMa has abnormal neuroanatomy phenotype, suppressible by spas^RNAi.UAS/Scer\GAL4²²¹

(Jinushi-Nakao et al., 2007)

Enhancer of

Statement

Reference

kn^UAS.cMa/Scer\GAL4²²¹ is an enhancer of abnormal neuroanatomy phenotype of Scer\GAL4²²¹, ct^UAS.cPa

(Jinushi-Nakao et al., 2007)

NOT Suppressor of

Statement

Reference

kn^UAS.cMa/Scer\GAL4^21-7 is a non-suppressor of abnormal neuroanatomy phenotype of dar1³⁰¹⁰

(Ye et al., 2011)

Other

Statement

Reference

Scer\GAL4²²¹, kn^UAS.cMa, spas^RNAi.UAS has abnormal neuroanatomy phenotype

(Jinushi-Nakao et al., 2007)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4²²¹, kn^UAS.cMa has larval multidendritic class I neuron phenotype, enhanceable by Rac1^UAS.cLa, Scer\GAL4²²¹

(Jinushi-Nakao et al., 2007)

Scer\GAL4²²¹, kn^UAS.cMa has dendrite phenotype, enhanceable by Rac1^UAS.cLa, Scer\GAL4²²¹

(Jinushi-Nakao et al., 2007)

Scer\GAL4²²¹, kn^UAS.cMa has larval multidendritic class I neuron phenotype, enhanceable by ct^UAS.cPa/Scer\GAL4²²¹

(Jinushi-Nakao et al., 2007)

Scer\GAL4²²¹, kn^UAS.cMa has dendrite phenotype, enhanceable by ct^UAS.cPa/Scer\GAL4²²¹

(Jinushi-Nakao et al., 2007)

Suppressed by

Statement

Reference

Scer\GAL4^21-7, kn^UAS.cMa has dendritic arborizing neuron | somatic clone phenotype, suppressible by dar1³⁰¹⁰

(Ye et al., 2011)

Scer\GAL4²²¹, kn^UAS.cMa has larval multidendritic class I neuron phenotype, suppressible by spas^RNAi.UAS/Scer\GAL4²²¹

(Jinushi-Nakao et al., 2007)

Scer\GAL4²²¹, kn^UAS.cMa has dendrite phenotype, suppressible by spas^RNAi.UAS/Scer\GAL4²²¹

(Jinushi-Nakao et al., 2007)

Enhancer of

Statement

Reference

kn^UAS.cMa/Scer\GAL4²²¹ is an enhancer of larval multidendritic class I neuron phenotype of Scer\GAL4²²¹, ct^UAS.cPa

(Jinushi-Nakao et al., 2007)

kn^UAS.cMa/Scer\GAL4²²¹ is an enhancer of dendrite phenotype of Scer\GAL4²²¹, ct^UAS.cPa

(Jinushi-Nakao et al., 2007)

Suppressor of

Statement

Reference

kn^UAS.cMa, Scer\GAL4^bi-md653 is a suppressor of wing cell phenotype of Rbfox1^RNAi.UAS, Scer\GAL4^bi-md653

(Usha and Shashidhara, 2010)

NOT Suppressor of

Statement

Reference

kn^UAS.cMa/Scer\GAL4^21-7 is a non-suppressor of dendritic arborizing neuron phenotype of dar1³⁰¹⁰

(Ye et al., 2011)

Other

Statement

Reference

Rbfox1^RNAi.UAS, Scer\GAL4^bi-md653, kn^UAS.cMa has wing vein phenotype

(Usha and Shashidhara, 2010)

Scer\GAL4²²¹, kn^UAS.cMa, spas^RNAi.UAS has dendrite phenotype

(Jinushi-Nakao et al., 2007)

Scer\GAL4²²¹, kn^UAS.cMa, spas^RNAi.UAS has larval dorsal multidendritic neuron ddaC phenotype

(Jinushi-Nakao et al., 2007)

Scer\GAL4²²¹, kn^UAS.cMa, spas^RNAi.UAS has larval multidendritic class IV neuron phenotype

(Jinushi-Nakao et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

A dar1³⁰¹⁰ mutant background suppresses the increase in dendrite length found upon expression of kn^Scer\UAS.cMa in class I da neurons under the control of Scer\GAL4^21-7 and results in a severe reduction in dendrite length.

(Ye et al., 2011)

Co-expression of kn^Scer\UAS.cMa in a Scer\GAL4^bi-md653, A2bp1^{dsRNA.Scer\UAS} background rescues the loss of intervein tissue phenotype and causes a loss of vein tissue.

(Usha and Shashidhara, 2010)

Ectopic expression of Rac1^Scer\UAS.cLa along with kn^Scer\UAS.cMa in class I neurons under the control of Scer\GAL4²²¹ promotes the outgrowth of bona fide dendrites from the thorn-shaped projections initiated by Rac1^Scer\UAS.cLa expression. In addition, coexpression of kn^Scer\UAS.cMa causes a small, significant reduction in termini number.

Co-expression of kn^Scer\UAS.cMa and ct^Scer\UAS.cPa under the control of Scer\GAL4²²¹ increases the number of dendritic branching points to 409% of wild-type. Similarly, the total length is increased by 130% and the arbor area by 89%.

Ectopic expression of spas^{dsRNA.Scer\UAS} strongly reduces both branching and total dendrite length by 18 and 19% respectively, when expressed in kn^Scer\UAS.cMa-expressing class I neurons (both transgenes under the control of Scer\GAL4²²¹).

(Jinushi-Nakao et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

kn^Scer\UAS.cMa

kn^UAS.cMa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Mohler

(Mohler et al., 2000)

Secondary FlyBase IDs

References (8)

Report Sections

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